Amino acid dipepetide frequency for Streptomyces sp. Ru73

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.151AlaAla: 24.151 ± 0.16	1.097AlaCys: 1.097 ± 0.024	8.657AlaAsp: 8.657 ± 0.075	9.224AlaGlu: 9.224 ± 0.08	3.555AlaPhe: 3.555 ± 0.041	14.447AlaGly: 14.447 ± 0.087	3.119AlaHis: 3.119 ± 0.037	3.24AlaIle: 3.24 ± 0.043	2.953AlaLys: 2.953 ± 0.051	14.821AlaLeu: 14.821 ± 0.101	2.545AlaMet: 2.545 ± 0.034	1.832AlaAsn: 1.832 ± 0.03	7.878AlaPro: 7.878 ± 0.082	3.744AlaGln: 3.744 ± 0.047	10.904AlaArg: 10.904 ± 0.089	5.42AlaSer: 5.42 ± 0.05	7.014AlaThr: 7.014 ± 0.068	12.582AlaVal: 12.582 ± 0.092	1.8AlaTrp: 1.8 ± 0.029	2.868AlaTyr: 2.868 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.206CysAla: 1.206 ± 0.026	0.096CysCys: 0.096 ± 0.007	0.458CysAsp: 0.458 ± 0.015	0.379CysGlu: 0.379 ± 0.014	0.254CysPhe: 0.254 ± 0.011	1.025CysGly: 1.025 ± 0.022	0.203CysHis: 0.203 ± 0.011	0.198CysIle: 0.198 ± 0.01	0.122CysLys: 0.122 ± 0.007	0.758CysLeu: 0.758 ± 0.019	0.129CysMet: 0.129 ± 0.007	0.122CysAsn: 0.122 ± 0.007	0.502CysPro: 0.502 ± 0.013	0.156CysGln: 0.156 ± 0.007	0.65CysArg: 0.65 ± 0.019	0.408CysSer: 0.408 ± 0.015	0.535CysThr: 0.535 ± 0.015	0.664CysVal: 0.664 ± 0.017	0.122CysTrp: 0.122 ± 0.007	0.17CysTyr: 0.17 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.93AspAla: 7.93 ± 0.068	0.414AspCys: 0.414 ± 0.013	3.347AspAsp: 3.347 ± 0.042	3.697AspGlu: 3.697 ± 0.041	1.633AspPhe: 1.633 ± 0.028	6.556AspGly: 6.556 ± 0.06	1.454AspHis: 1.454 ± 0.027	1.812AspIle: 1.812 ± 0.03	1.153AspLys: 1.153 ± 0.026	5.992AspLeu: 5.992 ± 0.05	0.845AspMet: 0.845 ± 0.018	0.873AspAsn: 0.873 ± 0.021	4.397AspPro: 4.397 ± 0.053	1.426AspGln: 1.426 ± 0.024	5.123AspArg: 5.123 ± 0.055	2.233AspSer: 2.233 ± 0.035	3.148AspThr: 3.148 ± 0.035	4.557AspVal: 4.557 ± 0.051	0.97AspTrp: 0.97 ± 0.021	1.044AspTyr: 1.044 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.666GluAla: 7.666 ± 0.081	0.386GluCys: 0.386 ± 0.015	2.914GluAsp: 2.914 ± 0.04	3.733GluGlu: 3.733 ± 0.05	1.37GluPhe: 1.37 ± 0.024	4.297GluGly: 4.297 ± 0.048	1.536GluHis: 1.536 ± 0.025	2.127GluIle: 2.127 ± 0.036	1.523GluLys: 1.523 ± 0.034	6.718GluLeu: 6.718 ± 0.062	0.899GluMet: 0.899 ± 0.022	0.964GluAsn: 0.964 ± 0.021	3.393GluPro: 3.393 ± 0.047	2.163GluGln: 2.163 ± 0.031	5.573GluArg: 5.573 ± 0.06	2.321GluSer: 2.321 ± 0.031	2.878GluThr: 2.878 ± 0.035	4.393GluVal: 4.393 ± 0.046	0.82GluTrp: 0.82 ± 0.018	1.127GluTyr: 1.127 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.833PheAla: 3.833 ± 0.048	0.278PheCys: 0.278 ± 0.012	1.833PheAsp: 1.833 ± 0.03	1.353PheGlu: 1.353 ± 0.021	0.944PhePhe: 0.944 ± 0.022	3.001PheGly: 3.001 ± 0.036	0.636PheHis: 0.636 ± 0.017	0.726PheIle: 0.726 ± 0.018	0.502PheLys: 0.502 ± 0.017	2.696PheLeu: 2.696 ± 0.042	0.41PheMet: 0.41 ± 0.013	0.53PheAsn: 0.53 ± 0.018	1.43PhePro: 1.43 ± 0.025	0.654PheGln: 0.654 ± 0.016	1.862PheArg: 1.862 ± 0.029	1.369PheSer: 1.369 ± 0.029	1.981PheThr: 1.981 ± 0.031	2.138PheVal: 2.138 ± 0.03	0.449PheTrp: 0.449 ± 0.014	0.572PheTyr: 0.572 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.915GlyAla: 11.915 ± 0.084	0.875GlyCys: 0.875 ± 0.02	5.082GlyAsp: 5.082 ± 0.051	5.186GlyGlu: 5.186 ± 0.049	2.909GlyPhe: 2.909 ± 0.038	9.316GlyGly: 9.316 ± 0.099	2.421GlyHis: 2.421 ± 0.033	3.555GlyIle: 3.555 ± 0.042	2.547GlyLys: 2.547 ± 0.049	9.732GlyLeu: 9.732 ± 0.071	2.073GlyMet: 2.073 ± 0.032	1.667GlyAsn: 1.667 ± 0.026	5.713GlyPro: 5.713 ± 0.061	2.639GlyGln: 2.639 ± 0.04	8.352GlyArg: 8.352 ± 0.057	5.165GlySer: 5.165 ± 0.052	6.651GlyThr: 6.651 ± 0.058	7.367GlyVal: 7.367 ± 0.059	1.66GlyTrp: 1.66 ± 0.028	2.33GlyTyr: 2.33 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	2.831HisAla: 2.831 ± 0.036	0.233HisCys: 0.233 ± 0.011	1.396HisAsp: 1.396 ± 0.025	1.264HisGlu: 1.264 ± 0.025	0.694HisPhe: 0.694 ± 0.019	2.666HisGly: 2.666 ± 0.037	0.748HisHis: 0.748 ± 0.021	0.681HisIle: 0.681 ± 0.019	0.38HisLys: 0.38 ± 0.013	2.489HisLeu: 2.489 ± 0.029	0.336HisMet: 0.336 ± 0.012	0.388HisAsn: 0.388 ± 0.014	1.888HisPro: 1.888 ± 0.032	0.636HisGln: 0.636 ± 0.017	2.217HisArg: 2.217 ± 0.033	0.981HisSer: 0.981 ± 0.025	1.408HisThr: 1.408 ± 0.026	1.693HisVal: 1.693 ± 0.028	0.395HisTrp: 0.395 ± 0.014	0.529HisTyr: 0.529 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.684IleAla: 4.684 ± 0.057	0.3IleCys: 0.3 ± 0.012	2.111IleAsp: 2.111 ± 0.029	1.998IleGlu: 1.998 ± 0.036	0.697IlePhe: 0.697 ± 0.02	3.704IleGly: 3.704 ± 0.047	0.584IleHis: 0.584 ± 0.016	0.859IleIle: 0.859 ± 0.021	0.747IleLys: 0.747 ± 0.018	2.203IleLeu: 2.203 ± 0.031	0.439IleMet: 0.439 ± 0.014	0.661IleAsn: 0.661 ± 0.019	1.705IlePro: 1.705 ± 0.027	0.687IleGln: 0.687 ± 0.019	2.094IleArg: 2.094 ± 0.035	1.562IleSer: 1.562 ± 0.027	2.096IleThr: 2.096 ± 0.034	2.61IleVal: 2.61 ± 0.042	0.356IleTrp: 0.356 ± 0.013	0.502IleTyr: 0.502 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	2.871LysAla: 2.871 ± 0.055	0.109LysCys: 0.109 ± 0.007	1.451LysAsp: 1.451 ± 0.035	1.286LysGlu: 1.286 ± 0.027	0.458LysPhe: 0.458 ± 0.014	1.906LysGly: 1.906 ± 0.038	0.466LysHis: 0.466 ± 0.013	0.855LysIle: 0.855 ± 0.022	1.021LysLys: 1.021 ± 0.031	2.064LysLeu: 2.064 ± 0.036	0.405LysMet: 0.405 ± 0.015	0.527LysAsn: 0.527 ± 0.018	1.365LysPro: 1.365 ± 0.036	0.722LysGln: 0.722 ± 0.019	1.492LysArg: 1.492 ± 0.027	1.064LysSer: 1.064 ± 0.023	1.29LysThr: 1.29 ± 0.027	1.847LysVal: 1.847 ± 0.03	0.269LysTrp: 0.269 ± 0.01	0.476LysTyr: 0.476 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.774LeuAla: 15.774 ± 0.103	0.881LeuCys: 0.881 ± 0.021	6.492LeuAsp: 6.492 ± 0.056	4.482LeuGlu: 4.482 ± 0.049	2.654LeuPhe: 2.654 ± 0.038	9.429LeuGly: 9.429 ± 0.069	2.404LeuHis: 2.404 ± 0.035	3.27LeuIle: 3.27 ± 0.041	2.003LeuLys: 2.003 ± 0.029	11.398LeuLeu: 11.398 ± 0.097	1.602LeuMet: 1.602 ± 0.031	1.624LeuAsn: 1.624 ± 0.029	6.681LeuPro: 6.681 ± 0.057	2.216LeuGln: 2.216 ± 0.026	8.813LeuArg: 8.813 ± 0.072	5.211LeuSer: 5.211 ± 0.047	6.919LeuThr: 6.919 ± 0.064	8.527LeuVal: 8.527 ± 0.07	1.255LeuTrp: 1.255 ± 0.025	1.872LeuTyr: 1.872 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.383MetAla: 2.383 ± 0.028	0.148MetCys: 0.148 ± 0.008	0.892MetAsp: 0.892 ± 0.018	0.708MetGlu: 0.708 ± 0.016	0.437MetPhe: 0.437 ± 0.015	1.373MetGly: 1.373 ± 0.027	0.372MetHis: 0.372 ± 0.012	0.651MetIle: 0.651 ± 0.015	0.424MetLys: 0.424 ± 0.015	1.704MetLeu: 1.704 ± 0.029	0.308MetMet: 0.308 ± 0.012	0.416MetAsn: 0.416 ± 0.012	1.133MetPro: 1.133 ± 0.023	0.449MetGln: 0.449 ± 0.014	1.418MetArg: 1.418 ± 0.023	1.242MetSer: 1.242 ± 0.023	1.541MetThr: 1.541 ± 0.029	1.271MetVal: 1.271 ± 0.025	0.216MetTrp: 0.216 ± 0.011	0.31MetTyr: 0.31 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.128AsnAla: 2.128 ± 0.033	0.156AsnCys: 0.156 ± 0.009	0.928AsnAsp: 0.928 ± 0.022	0.81AsnGlu: 0.81 ± 0.021	0.478AsnPhe: 0.478 ± 0.015	1.785AsnGly: 1.785 ± 0.031	0.384AsnHis: 0.384 ± 0.012	0.602AsnIle: 0.602 ± 0.019	0.408AsnLys: 0.408 ± 0.016	1.55AsnLeu: 1.55 ± 0.026	0.287AsnMet: 0.287 ± 0.011	0.396AsnAsn: 0.396 ± 0.014	1.255AsnPro: 1.255 ± 0.027	0.433AsnGln: 0.433 ± 0.013	1.135AsnArg: 1.135 ± 0.021	0.841AsnSer: 0.841 ± 0.021	1.039AsnThr: 1.039 ± 0.024	1.352AsnVal: 1.352 ± 0.029	0.258AsnTrp: 0.258 ± 0.011	0.384AsnTyr: 0.384 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	9.518ProAla: 9.518 ± 0.084	0.368ProCys: 0.368 ± 0.012	4.584ProAsp: 4.584 ± 0.051	4.654ProGlu: 4.654 ± 0.049	1.539ProPhe: 1.539 ± 0.032	7.0ProGly: 7.0 ± 0.075	1.569ProHis: 1.569 ± 0.028	1.104ProIle: 1.104 ± 0.023	1.267ProLys: 1.267 ± 0.033	5.517ProLeu: 5.517 ± 0.056	1.041ProMet: 1.041 ± 0.023	0.839ProAsn: 0.839 ± 0.022	3.569ProPro: 3.569 ± 0.054	1.845ProGln: 1.845 ± 0.038	4.084ProArg: 4.084 ± 0.047	2.888ProSer: 2.888 ± 0.043	2.915ProThr: 2.915 ± 0.038	5.627ProVal: 5.627 ± 0.05	0.878ProTrp: 0.878 ± 0.023	1.536ProTyr: 1.536 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	3.582GlnAla: 3.582 ± 0.046	0.18GlnCys: 0.18 ± 0.009	1.381GlnAsp: 1.381 ± 0.025	1.497GlnGlu: 1.497 ± 0.025	0.677GlnPhe: 0.677 ± 0.018	2.273GlnGly: 2.273 ± 0.037	0.744GlnHis: 0.744 ± 0.02	1.0GlnIle: 1.0 ± 0.023	0.62GlnLys: 0.62 ± 0.014	3.019GlnLeu: 3.019 ± 0.037	0.485GlnMet: 0.485 ± 0.015	0.47GlnAsn: 0.47 ± 0.015	1.724GlnPro: 1.724 ± 0.041	1.353GlnGln: 1.353 ± 0.038	2.425GlnArg: 2.425 ± 0.03	1.122GlnSer: 1.122 ± 0.021	1.227GlnThr: 1.227 ± 0.025	2.193GlnVal: 2.193 ± 0.037	0.456GlnTrp: 0.456 ± 0.014	0.636GlnTyr: 0.636 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.518ArgAla: 10.518 ± 0.08	0.621ArgCys: 0.621 ± 0.017	4.321ArgAsp: 4.321 ± 0.047	4.759ArgGlu: 4.759 ± 0.05	2.247ArgPhe: 2.247 ± 0.034	6.078ArgGly: 6.078 ± 0.058	2.145ArgHis: 2.145 ± 0.034	3.263ArgIle: 3.263 ± 0.038	1.756ArgLys: 1.756 ± 0.032	8.938ArgLeu: 8.938 ± 0.068	1.731ArgMet: 1.731 ± 0.028	1.356ArgAsn: 1.356 ± 0.025	5.298ArgPro: 5.298 ± 0.054	2.312ArgGln: 2.312 ± 0.034	7.944ArgArg: 7.944 ± 0.081	3.887ArgSer: 3.887 ± 0.045	5.824ArgThr: 5.824 ± 0.052	5.553ArgVal: 5.553 ± 0.054	1.312ArgTrp: 1.312 ± 0.026	1.884ArgTyr: 1.884 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.444SerAla: 6.444 ± 0.052	0.416SerCys: 0.416 ± 0.012	2.39SerAsp: 2.39 ± 0.037	2.225SerGlu: 2.225 ± 0.031	1.492SerPhe: 1.492 ± 0.028	5.695SerGly: 5.695 ± 0.05	0.997SerHis: 0.997 ± 0.023	1.269SerIle: 1.269 ± 0.025	0.984SerLys: 0.984 ± 0.024	4.476SerLeu: 4.476 ± 0.043	0.988SerMet: 0.988 ± 0.022	0.75SerAsn: 0.75 ± 0.019	3.017SerPro: 3.017 ± 0.037	1.159SerGln: 1.159 ± 0.027	3.431SerArg: 3.431 ± 0.041	2.503SerSer: 2.503 ± 0.041	2.644SerThr: 2.644 ± 0.033	3.92SerVal: 3.92 ± 0.042	0.821SerTrp: 0.821 ± 0.017	1.153SerTyr: 1.153 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.315ThrAla: 9.315 ± 0.077	0.431ThrCys: 0.431 ± 0.014	3.574ThrAsp: 3.574 ± 0.038	3.315ThrGlu: 3.315 ± 0.038	1.586ThrPhe: 1.586 ± 0.027	6.928ThrGly: 6.928 ± 0.07	1.24ThrHis: 1.24 ± 0.024	1.589ThrIle: 1.589 ± 0.028	1.177ThrLys: 1.177 ± 0.028	5.622ThrLeu: 5.622 ± 0.054	0.958ThrMet: 0.958 ± 0.021	0.865ThrAsn: 0.865 ± 0.023	4.072ThrPro: 4.072 ± 0.048	1.239ThrGln: 1.239 ± 0.026	3.78ThrArg: 3.78 ± 0.041	2.761ThrSer: 2.761 ± 0.039	3.62ThrThr: 3.62 ± 0.052	6.002ThrVal: 6.002 ± 0.054	0.843ThrTrp: 0.843 ± 0.022	1.337ThrTyr: 1.337 ± 0.023	0.0ThrXaa: 0.0 ± 0.0
Val	10.6ValAla: 10.6 ± 0.07	0.833ValCys: 0.833 ± 0.018	4.488ValAsp: 4.488 ± 0.052	4.26ValGlu: 4.26 ± 0.05	2.396ValPhe: 2.396 ± 0.034	6.207ValGly: 6.207 ± 0.055	2.013ValHis: 2.013 ± 0.03	2.828ValIle: 2.828 ± 0.039	1.617ValLys: 1.617 ± 0.035	9.84ValLeu: 9.84 ± 0.069	1.369ValMet: 1.369 ± 0.023	1.604ValAsn: 1.604 ± 0.032	5.478ValPro: 5.478 ± 0.045	2.039ValGln: 2.039 ± 0.03	7.274ValArg: 7.274 ± 0.055	4.046ValSer: 4.046 ± 0.042	5.435ValThr: 5.435 ± 0.054	7.547ValVal: 7.547 ± 0.07	1.077ValTrp: 1.077 ± 0.022	1.508ValTyr: 1.508 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.59TrpAla: 1.59 ± 0.027	0.149TrpCys: 0.149 ± 0.008	0.794TrpAsp: 0.794 ± 0.019	0.7TrpGlu: 0.7 ± 0.02	0.512TrpPhe: 0.512 ± 0.016	1.057TrpGly: 1.057 ± 0.021	0.407TrpHis: 0.407 ± 0.012	0.5TrpIle: 0.5 ± 0.016	0.337TrpLys: 0.337 ± 0.013	1.779TrpLeu: 1.779 ± 0.034	0.288TrpMet: 0.288 ± 0.011	0.351TrpAsn: 0.351 ± 0.013	0.773TrpPro: 0.773 ± 0.021	0.627TrpGln: 0.627 ± 0.018	1.331TrpArg: 1.331 ± 0.025	0.84TrpSer: 0.84 ± 0.019	0.978TrpThr: 0.978 ± 0.022	0.929TrpVal: 0.929 ± 0.024	0.325TrpTrp: 0.325 ± 0.013	0.358TrpTyr: 0.358 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.912TyrAla: 2.912 ± 0.036	0.19TyrCys: 0.19 ± 0.009	1.509TyrAsp: 1.509 ± 0.032	1.271TyrGlu: 1.271 ± 0.023	0.666TyrPhe: 0.666 ± 0.016	2.41TyrGly: 2.41 ± 0.035	0.411TyrHis: 0.411 ± 0.013	0.468TyrIle: 0.468 ± 0.014	0.403TyrLys: 0.403 ± 0.015	2.19TyrLeu: 2.19 ± 0.029	0.258TyrMet: 0.258 ± 0.013	0.372TyrAsn: 0.372 ± 0.013	1.075TyrPro: 1.075 ± 0.024	0.567TyrGln: 0.567 ± 0.015	1.845TyrArg: 1.845 ± 0.03	0.911TyrSer: 0.911 ± 0.019	1.111TyrThr: 1.111 ± 0.024	1.685TyrVal: 1.685 ± 0.029	0.342TyrTrp: 0.342 ± 0.013	0.471TyrTyr: 0.471 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7006 proteins (2295001 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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