Amino acid dipepetide frequency for Rhodovarius sp. CCP-6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.745AlaAla: 21.745 ± 0.18
1.206AlaCys: 1.206 ± 0.031
6.422AlaAsp: 6.422 ± 0.067
9.239AlaGlu: 9.239 ± 0.092
4.261AlaPhe: 4.261 ± 0.058
13.012AlaGly: 13.012 ± 0.099
2.516AlaHis: 2.516 ± 0.043
5.699AlaIle: 5.699 ± 0.065
2.954AlaLys: 2.954 ± 0.047
16.166AlaLeu: 16.166 ± 0.121
4.505AlaMet: 4.505 ± 0.055
2.787AlaAsn: 2.787 ± 0.046
7.843AlaPro: 7.843 ± 0.103
4.866AlaGln: 4.866 ± 0.056
11.443AlaArg: 11.443 ± 0.109
6.024AlaSer: 6.024 ± 0.064
6.578AlaThr: 6.578 ± 0.069
9.301AlaVal: 9.301 ± 0.081
2.065AlaTrp: 2.065 ± 0.038
2.277AlaTyr: 2.277 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.045CysAla: 1.045 ± 0.027
0.129CysCys: 0.129 ± 0.01
0.468CysAsp: 0.468 ± 0.015
0.376CysGlu: 0.376 ± 0.016
0.326CysPhe: 0.326 ± 0.013
0.902CysGly: 0.902 ± 0.026
0.232CysHis: 0.232 ± 0.014
0.383CysIle: 0.383 ± 0.016
0.128CysLys: 0.128 ± 0.009
0.824CysLeu: 0.824 ± 0.025
0.186CysMet: 0.186 ± 0.013
0.197CysAsn: 0.197 ± 0.011
0.498CysPro: 0.498 ± 0.017
0.201CysGln: 0.201 ± 0.01
0.681CysArg: 0.681 ± 0.023
0.396CysSer: 0.396 ± 0.015
0.476CysThr: 0.476 ± 0.017
0.641CysVal: 0.641 ± 0.019
0.136CysTrp: 0.136 ± 0.009
0.185CysTyr: 0.185 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.174AspAla: 7.174 ± 0.08
0.412AspCys: 0.412 ± 0.016
2.281AspAsp: 2.281 ± 0.042
2.591AspGlu: 2.591 ± 0.04
1.832AspPhe: 1.832 ± 0.038
4.858AspGly: 4.858 ± 0.065
1.007AspHis: 1.007 ± 0.023
2.498AspIle: 2.498 ± 0.041
0.983AspLys: 0.983 ± 0.027
4.813AspLeu: 4.813 ± 0.051
1.287AspMet: 1.287 ± 0.028
1.045AspAsn: 1.045 ± 0.026
3.469AspPro: 3.469 ± 0.053
1.435AspGln: 1.435 ± 0.037
3.767AspArg: 3.767 ± 0.049
1.85AspSer: 1.85 ± 0.036
2.404AspThr: 2.404 ± 0.049
3.693AspVal: 3.693 ± 0.049
0.969AspTrp: 0.969 ± 0.025
1.157AspTyr: 1.157 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
9.148GluAla: 9.148 ± 0.1
0.368GluCys: 0.368 ± 0.016
2.465GluAsp: 2.465 ± 0.049
3.295GluGlu: 3.295 ± 0.057
1.675GluPhe: 1.675 ± 0.034
4.95GluGly: 4.95 ± 0.053
1.12GluHis: 1.12 ± 0.024
2.631GluIle: 2.631 ± 0.038
1.562GluLys: 1.562 ± 0.036
5.277GluLeu: 5.277 ± 0.067
1.619GluMet: 1.619 ± 0.035
1.31GluAsn: 1.31 ± 0.03
2.847GluPro: 2.847 ± 0.047
2.079GluGln: 2.079 ± 0.039
4.773GluArg: 4.773 ± 0.071
1.976GluSer: 1.976 ± 0.039
2.954GluThr: 2.954 ± 0.042
3.908GluVal: 3.908 ± 0.049
0.804GluTrp: 0.804 ± 0.022
0.896GluTyr: 0.896 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
4.401PheAla: 4.401 ± 0.051
0.384PheCys: 0.384 ± 0.015
2.113PheAsp: 2.113 ± 0.033
1.727PheGlu: 1.727 ± 0.03
1.13PhePhe: 1.13 ± 0.03
3.472PheGly: 3.472 ± 0.05
0.771PheHis: 0.771 ± 0.02
1.358PheIle: 1.358 ± 0.032
0.585PheLys: 0.585 ± 0.019
3.284PheLeu: 3.284 ± 0.047
0.779PheMet: 0.779 ± 0.024
0.937PheAsn: 0.937 ± 0.024
1.72PhePro: 1.72 ± 0.039
1.061PheGln: 1.061 ± 0.022
2.417PheArg: 2.417 ± 0.039
1.929PheSer: 1.929 ± 0.039
2.025PheThr: 2.025 ± 0.036
2.255PheVal: 2.255 ± 0.04
0.546PheTrp: 0.546 ± 0.022
0.687PheTyr: 0.687 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.164GlyAla: 11.164 ± 0.088
0.902GlyCys: 0.902 ± 0.025
3.931GlyAsp: 3.931 ± 0.057
4.646GlyGlu: 4.646 ± 0.05
3.899GlyPhe: 3.899 ± 0.052
8.759GlyGly: 8.759 ± 0.126
2.036GlyHis: 2.036 ± 0.032
4.362GlyIle: 4.362 ± 0.053
2.341GlyLys: 2.341 ± 0.045
10.008GlyLeu: 10.008 ± 0.101
2.956GlyMet: 2.956 ± 0.045
2.192GlyAsn: 2.192 ± 0.046
4.325GlyPro: 4.325 ± 0.049
3.393GlyGln: 3.393 ± 0.046
7.341GlyArg: 7.341 ± 0.066
4.681GlySer: 4.681 ± 0.061
5.205GlyThr: 5.205 ± 0.069
6.574GlyVal: 6.574 ± 0.069
1.779GlyTrp: 1.779 ± 0.041
2.128GlyTyr: 2.128 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.76HisAla: 2.76 ± 0.039
0.219HisCys: 0.219 ± 0.012
1.118HisAsp: 1.118 ± 0.029
1.002HisGlu: 1.002 ± 0.027
0.771HisPhe: 0.771 ± 0.022
2.159HisGly: 2.159 ± 0.043
0.594HisHis: 0.594 ± 0.024
0.897HisIle: 0.897 ± 0.024
0.366HisLys: 0.366 ± 0.016
2.081HisLeu: 2.081 ± 0.037
0.49HisMet: 0.49 ± 0.016
0.428HisAsn: 0.428 ± 0.016
1.544HisPro: 1.544 ± 0.038
0.572HisGln: 0.572 ± 0.017
1.471HisArg: 1.471 ± 0.032
0.874HisSer: 0.874 ± 0.024
0.856HisThr: 0.856 ± 0.025
1.575HisVal: 1.575 ± 0.031
0.391HisTrp: 0.391 ± 0.015
0.471HisTyr: 0.471 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.469IleAla: 6.469 ± 0.068
0.45IleCys: 0.45 ± 0.015
2.3IleAsp: 2.3 ± 0.042
2.825IleGlu: 2.825 ± 0.043
1.255IlePhe: 1.255 ± 0.03
4.35IleGly: 4.35 ± 0.051
0.829IleHis: 0.829 ± 0.022
1.772IleIle: 1.772 ± 0.034
0.861IleLys: 0.861 ± 0.027
3.888IleLeu: 3.888 ± 0.048
0.948IleMet: 0.948 ± 0.025
1.098IleAsn: 1.098 ± 0.029
2.464IlePro: 2.464 ± 0.038
1.236IleGln: 1.236 ± 0.027
3.334IleArg: 3.334 ± 0.043
2.266IleSer: 2.266 ± 0.042
2.6IleThr: 2.6 ± 0.044
3.076IleVal: 3.076 ± 0.047
0.539IleTrp: 0.539 ± 0.018
0.747IleTyr: 0.747 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
3.017LysAla: 3.017 ± 0.053
0.103LysCys: 0.103 ± 0.008
1.052LysAsp: 1.052 ± 0.029
1.117LysGlu: 1.117 ± 0.029
0.572LysPhe: 0.572 ± 0.019
1.967LysGly: 1.967 ± 0.039
0.447LysHis: 0.447 ± 0.015
0.966LysIle: 0.966 ± 0.027
0.697LysLys: 0.697 ± 0.028
2.582LysLeu: 2.582 ± 0.041
0.469LysMet: 0.469 ± 0.016
0.498LysAsn: 0.498 ± 0.018
1.674LysPro: 1.674 ± 0.033
0.675LysGln: 0.675 ± 0.022
1.777LysArg: 1.777 ± 0.04
0.986LysSer: 0.986 ± 0.027
1.0LysThr: 1.0 ± 0.027
1.663LysVal: 1.663 ± 0.037
0.255LysTrp: 0.255 ± 0.012
0.356LysTyr: 0.356 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
16.196LeuAla: 16.196 ± 0.122
0.97LeuCys: 0.97 ± 0.022
5.699LeuAsp: 5.699 ± 0.064
5.407LeuGlu: 5.407 ± 0.068
3.169LeuPhe: 3.169 ± 0.05
9.504LeuGly: 9.504 ± 0.092
2.165LeuHis: 2.165 ± 0.039
3.921LeuIle: 3.921 ± 0.052
2.557LeuLys: 2.557 ± 0.047
11.151LeuLeu: 11.151 ± 0.126
2.578LeuMet: 2.578 ± 0.04
2.524LeuAsn: 2.524 ± 0.043
7.17LeuPro: 7.17 ± 0.079
2.597LeuGln: 2.597 ± 0.037
8.834LeuArg: 8.834 ± 0.093
5.944LeuSer: 5.944 ± 0.065
5.425LeuThr: 5.425 ± 0.062
7.312LeuVal: 7.312 ± 0.077
1.337LeuTrp: 1.337 ± 0.029
1.74LeuTyr: 1.74 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
3.866MetAla: 3.866 ± 0.05
0.188MetCys: 0.188 ± 0.009
1.235MetAsp: 1.235 ± 0.027
1.324MetGlu: 1.324 ± 0.026
0.679MetPhe: 0.679 ± 0.022
2.216MetGly: 2.216 ± 0.034
0.44MetHis: 0.44 ± 0.016
1.103MetIle: 1.103 ± 0.029
0.694MetLys: 0.694 ± 0.018
3.003MetLeu: 3.003 ± 0.045
0.675MetMet: 0.675 ± 0.021
0.741MetAsn: 0.741 ± 0.021
1.945MetPro: 1.945 ± 0.039
1.002MetGln: 1.002 ± 0.025
2.227MetArg: 2.227 ± 0.038
1.544MetSer: 1.544 ± 0.031
1.621MetThr: 1.621 ± 0.033
1.831MetVal: 1.831 ± 0.031
0.243MetTrp: 0.243 ± 0.012
0.301MetTyr: 0.301 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.983AsnAla: 2.983 ± 0.05
0.216AsnCys: 0.216 ± 0.011
1.135AsnAsp: 1.135 ± 0.033
1.026AsnGlu: 1.026 ± 0.027
0.806AsnPhe: 0.806 ± 0.022
2.152AsnGly: 2.152 ± 0.044
0.492AsnHis: 0.492 ± 0.018
1.17AsnIle: 1.17 ± 0.028
0.448AsnLys: 0.448 ± 0.017
2.433AsnLeu: 2.433 ± 0.041
0.551AsnMet: 0.551 ± 0.018
0.655AsnAsn: 0.655 ± 0.02
2.033AsnPro: 2.033 ± 0.033
0.652AsnGln: 0.652 ± 0.021
1.87AsnArg: 1.87 ± 0.036
1.098AsnSer: 1.098 ± 0.026
1.223AsnThr: 1.223 ± 0.029
1.615AsnVal: 1.615 ± 0.037
0.42AsnTrp: 0.42 ± 0.016
0.544AsnTyr: 0.544 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
8.87ProAla: 8.87 ± 0.1
0.385ProCys: 0.385 ± 0.016
3.814ProAsp: 3.814 ± 0.05
4.395ProGlu: 4.395 ± 0.056
2.11ProPhe: 2.11 ± 0.031
6.433ProGly: 6.433 ± 0.063
1.257ProHis: 1.257 ± 0.035
2.32ProIle: 2.32 ± 0.041
1.161ProLys: 1.161 ± 0.027
5.569ProLeu: 5.569 ± 0.063
1.522ProMet: 1.522 ± 0.037
1.385ProAsn: 1.385 ± 0.033
3.976ProPro: 3.976 ± 0.067
1.819ProGln: 1.819 ± 0.034
4.112ProArg: 4.112 ± 0.045
2.72ProSer: 2.72 ± 0.041
2.638ProThr: 2.638 ± 0.042
4.775ProVal: 4.775 ± 0.063
0.966ProTrp: 0.966 ± 0.024
1.245ProTyr: 1.245 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
5.11GlnAla: 5.11 ± 0.053
0.197GlnCys: 0.197 ± 0.011
1.499GlnAsp: 1.499 ± 0.03
1.636GlnGlu: 1.636 ± 0.034
0.932GlnPhe: 0.932 ± 0.023
3.139GlnGly: 3.139 ± 0.047
0.657GlnHis: 0.657 ± 0.021
1.38GlnIle: 1.38 ± 0.031
0.733GlnLys: 0.733 ± 0.023
2.888GlnLeu: 2.888 ± 0.048
0.88GlnMet: 0.88 ± 0.024
0.753GlnAsn: 0.753 ± 0.021
2.131GlnPro: 2.131 ± 0.041
1.294GlnGln: 1.294 ± 0.032
2.735GlnArg: 2.735 ± 0.047
1.467GlnSer: 1.467 ± 0.033
1.387GlnThr: 1.387 ± 0.031
2.459GlnVal: 2.459 ± 0.042
0.397GlnTrp: 0.397 ± 0.015
0.525GlnTyr: 0.525 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
10.392ArgAla: 10.392 ± 0.098
0.625ArgCys: 0.625 ± 0.019
4.182ArgAsp: 4.182 ± 0.052
4.543ArgGlu: 4.543 ± 0.057
3.053ArgPhe: 3.053 ± 0.045
6.121ArgGly: 6.121 ± 0.063
1.972ArgHis: 1.972 ± 0.035
3.849ArgIle: 3.849 ± 0.052
1.565ArgLys: 1.565 ± 0.034
9.347ArgLeu: 9.347 ± 0.096
2.242ArgMet: 2.242 ± 0.04
1.86ArgAsn: 1.86 ± 0.034
4.69ArgPro: 4.69 ± 0.05
2.928ArgGln: 2.928 ± 0.045
7.138ArgArg: 7.138 ± 0.085
3.347ArgSer: 3.347 ± 0.048
3.233ArgThr: 3.233 ± 0.045
5.498ArgVal: 5.498 ± 0.065
1.235ArgTrp: 1.235 ± 0.031
1.557ArgTyr: 1.557 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.145SerAla: 6.145 ± 0.062
0.403SerCys: 0.403 ± 0.017
2.17SerAsp: 2.17 ± 0.04
2.12SerGlu: 2.12 ± 0.039
1.96SerPhe: 1.96 ± 0.031
5.004SerGly: 5.004 ± 0.058
0.984SerHis: 0.984 ± 0.025
2.254SerIle: 2.254 ± 0.038
0.904SerLys: 0.904 ± 0.022
5.083SerLeu: 5.083 ± 0.066
1.251SerMet: 1.251 ± 0.028
1.071SerAsn: 1.071 ± 0.025
2.869SerPro: 2.869 ± 0.042
1.357SerGln: 1.357 ± 0.031
3.56SerArg: 3.56 ± 0.051
2.376SerSer: 2.376 ± 0.046
2.523SerThr: 2.523 ± 0.045
3.471SerVal: 3.471 ± 0.048
0.829SerTrp: 0.829 ± 0.024
0.997SerTyr: 0.997 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
6.457ThrAla: 6.457 ± 0.075
0.355ThrCys: 0.355 ± 0.013
2.633ThrAsp: 2.633 ± 0.039
2.683ThrGlu: 2.683 ± 0.044
1.544ThrPhe: 1.544 ± 0.034
5.166ThrGly: 5.166 ± 0.07
1.071ThrHis: 1.071 ± 0.025
2.396ThrIle: 2.396 ± 0.043
0.91ThrLys: 0.91 ± 0.029
5.957ThrLeu: 5.957 ± 0.069
1.182ThrMet: 1.182 ± 0.03
1.193ThrAsn: 1.193 ± 0.032
3.764ThrPro: 3.764 ± 0.05
1.472ThrGln: 1.472 ± 0.031
3.729ThrArg: 3.729 ± 0.047
2.43ThrSer: 2.43 ± 0.042
2.75ThrThr: 2.75 ± 0.049
3.864ThrVal: 3.864 ± 0.052
0.668ThrTrp: 0.668 ± 0.019
0.968ThrTyr: 0.968 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
9.903ValAla: 9.903 ± 0.084
0.543ValCys: 0.543 ± 0.018
3.235ValAsp: 3.235 ± 0.051
4.13ValGlu: 4.13 ± 0.052
2.406ValPhe: 2.406 ± 0.042
5.379ValGly: 5.379 ± 0.059
1.266ValHis: 1.266 ± 0.031
3.119ValIle: 3.119 ± 0.046
1.693ValLys: 1.693 ± 0.036
8.177ValLeu: 8.177 ± 0.077
1.982ValMet: 1.982 ± 0.036
1.892ValAsn: 1.892 ± 0.034
4.6ValPro: 4.6 ± 0.059
2.33ValGln: 2.33 ± 0.037
4.905ValArg: 4.905 ± 0.058
3.738ValSer: 3.738 ± 0.049
4.579ValThr: 4.579 ± 0.054
5.993ValVal: 5.993 ± 0.07
0.828ValTrp: 0.828 ± 0.022
1.065ValTyr: 1.065 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.604TrpAla: 1.604 ± 0.034
0.153TrpCys: 0.153 ± 0.011
0.641TrpAsp: 0.641 ± 0.023
0.615TrpGlu: 0.615 ± 0.02
0.565TrpPhe: 0.565 ± 0.02
1.193TrpGly: 1.193 ± 0.029
0.398TrpHis: 0.398 ± 0.017
0.539TrpIle: 0.539 ± 0.02
0.381TrpLys: 0.381 ± 0.016
1.865TrpLeu: 1.865 ± 0.037
0.4TrpMet: 0.4 ± 0.016
0.488TrpAsn: 0.488 ± 0.017
0.936TrpPro: 0.936 ± 0.026
0.675TrpGln: 0.675 ± 0.023
1.557TrpArg: 1.557 ± 0.033
0.728TrpSer: 0.728 ± 0.021
0.727TrpThr: 0.727 ± 0.02
0.953TrpVal: 0.953 ± 0.024
0.291TrpTrp: 0.291 ± 0.015
0.293TrpTyr: 0.293 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.46TyrAla: 2.46 ± 0.04
0.202TyrCys: 0.202 ± 0.01
1.13TyrAsp: 1.13 ± 0.028
0.962TyrGlu: 0.962 ± 0.025
0.633TyrPhe: 0.633 ± 0.019
1.889TyrGly: 1.889 ± 0.032
0.386TyrHis: 0.386 ± 0.018
0.687TyrIle: 0.687 ± 0.022
0.351TyrLys: 0.351 ± 0.016
1.804TyrLeu: 1.804 ± 0.033
0.375TyrMet: 0.375 ± 0.014
0.466TyrAsn: 0.466 ± 0.019
0.975TyrPro: 0.975 ± 0.028
0.557TyrGln: 0.557 ± 0.02
1.708TyrArg: 1.708 ± 0.029
0.956TyrSer: 0.956 ± 0.027
0.989TyrThr: 0.989 ± 0.025
1.309TyrVal: 1.309 ± 0.031
0.303TyrTrp: 0.303 ± 0.014
0.397TyrTyr: 0.397 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5028 proteins (1623387 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski