Amino acid dipepetide frequency for Paenibacillus sp. BK673

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.379AlaAla: 8.379 ± 0.095
0.738AlaCys: 0.738 ± 0.023
4.371AlaAsp: 4.371 ± 0.044
5.831AlaGlu: 5.831 ± 0.07
3.023AlaPhe: 3.023 ± 0.044
6.571AlaGly: 6.571 ± 0.065
1.483AlaHis: 1.483 ± 0.032
5.103AlaIle: 5.103 ± 0.056
3.736AlaLys: 3.736 ± 0.051
8.113AlaLeu: 8.113 ± 0.06
2.335AlaMet: 2.335 ± 0.034
2.628AlaAsn: 2.628 ± 0.046
2.784AlaPro: 2.784 ± 0.044
3.02AlaGln: 3.02 ± 0.047
3.713AlaArg: 3.713 ± 0.051
5.268AlaSer: 5.268 ± 0.063
4.163AlaThr: 4.163 ± 0.077
6.344AlaVal: 6.344 ± 0.055
0.965AlaTrp: 0.965 ± 0.023
2.766AlaTyr: 2.766 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.557CysAla: 0.557 ± 0.019
0.103CysCys: 0.103 ± 0.008
0.376CysAsp: 0.376 ± 0.015
0.467CysGlu: 0.467 ± 0.018
0.306CysPhe: 0.306 ± 0.013
0.713CysGly: 0.713 ± 0.02
0.207CysHis: 0.207 ± 0.012
0.553CysIle: 0.553 ± 0.018
0.321CysLys: 0.321 ± 0.014
0.74CysLeu: 0.74 ± 0.02
0.252CysMet: 0.252 ± 0.012
0.291CysAsn: 0.291 ± 0.014
0.353CysPro: 0.353 ± 0.016
0.241CysGln: 0.241 ± 0.012
0.427CysArg: 0.427 ± 0.015
0.583CysSer: 0.583 ± 0.019
0.468CysThr: 0.468 ± 0.017
0.495CysVal: 0.495 ± 0.016
0.097CysTrp: 0.097 ± 0.008
0.283CysTyr: 0.283 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.853AspAla: 3.853 ± 0.053
0.401AspCys: 0.401 ± 0.014
2.34AspAsp: 2.34 ± 0.04
3.843AspGlu: 3.843 ± 0.053
2.05AspPhe: 2.05 ± 0.039
3.893AspGly: 3.893 ± 0.056
1.164AspHis: 1.164 ± 0.024
3.606AspIle: 3.606 ± 0.049
2.53AspLys: 2.53 ± 0.039
4.654AspLeu: 4.654 ± 0.049
1.52AspMet: 1.52 ± 0.026
1.732AspAsn: 1.732 ± 0.031
2.342AspPro: 2.342 ± 0.039
2.211AspGln: 2.211 ± 0.036
2.8AspArg: 2.8 ± 0.043
2.99AspSer: 2.99 ± 0.041
2.813AspThr: 2.813 ± 0.042
3.526AspVal: 3.526 ± 0.049
0.825AspTrp: 0.825 ± 0.022
2.153AspTyr: 2.153 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
6.098GluAla: 6.098 ± 0.064
0.425GluCys: 0.425 ± 0.016
3.465GluAsp: 3.465 ± 0.051
5.575GluGlu: 5.575 ± 0.071
2.093GluPhe: 2.093 ± 0.039
4.626GluGly: 4.626 ± 0.051
2.012GluHis: 2.012 ± 0.038
4.12GluIle: 4.12 ± 0.05
3.295GluLys: 3.295 ± 0.047
8.133GluLeu: 8.133 ± 0.082
1.973GluMet: 1.973 ± 0.034
2.218GluAsn: 2.218 ± 0.037
2.369GluPro: 2.369 ± 0.037
4.705GluGln: 4.705 ± 0.07
4.286GluArg: 4.286 ± 0.058
3.524GluSer: 3.524 ± 0.042
2.997GluThr: 2.997 ± 0.048
4.681GluVal: 4.681 ± 0.051
0.92GluTrp: 0.92 ± 0.022
2.19GluTyr: 2.19 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
2.909PheAla: 2.909 ± 0.042
0.368PheCys: 0.368 ± 0.015
2.045PheAsp: 2.045 ± 0.039
2.345PheGlu: 2.345 ± 0.038
1.527PhePhe: 1.527 ± 0.036
2.916PheGly: 2.916 ± 0.045
0.83PheHis: 0.83 ± 0.019
2.629PheIle: 2.629 ± 0.043
1.761PheLys: 1.761 ± 0.033
3.433PheLeu: 3.433 ± 0.051
1.181PheMet: 1.181 ± 0.027
1.56PheAsn: 1.56 ± 0.033
1.457PhePro: 1.457 ± 0.03
1.345PheGln: 1.345 ± 0.025
1.979PheArg: 1.979 ± 0.037
2.538PheSer: 2.538 ± 0.041
2.322PheThr: 2.322 ± 0.039
2.708PheVal: 2.708 ± 0.042
0.488PheTrp: 0.488 ± 0.015
1.369PheTyr: 1.369 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
5.761GlyAla: 5.761 ± 0.079
0.687GlyCys: 0.687 ± 0.019
3.433GlyAsp: 3.433 ± 0.054
4.625GlyGlu: 4.625 ± 0.059
2.896GlyPhe: 2.896 ± 0.042
5.349GlyGly: 5.349 ± 0.072
1.564GlyHis: 1.564 ± 0.032
5.36GlyIle: 5.36 ± 0.068
3.922GlyLys: 3.922 ± 0.056
7.011GlyLeu: 7.011 ± 0.075
2.433GlyMet: 2.433 ± 0.038
2.636GlyAsn: 2.636 ± 0.053
2.005GlyPro: 2.005 ± 0.042
3.136GlyGln: 3.136 ± 0.044
3.642GlyArg: 3.642 ± 0.052
4.832GlySer: 4.832 ± 0.061
4.509GlyThr: 4.509 ± 0.065
5.194GlyVal: 5.194 ± 0.059
1.085GlyTrp: 1.085 ± 0.028
2.97GlyTyr: 2.97 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
1.738HisAla: 1.738 ± 0.034
0.224HisCys: 0.224 ± 0.009
1.091HisAsp: 1.091 ± 0.024
1.54HisGlu: 1.54 ± 0.029
0.982HisPhe: 0.982 ± 0.024
1.567HisGly: 1.567 ± 0.032
0.684HisHis: 0.684 ± 0.028
1.555HisIle: 1.555 ± 0.03
0.916HisLys: 0.916 ± 0.025
2.126HisLeu: 2.126 ± 0.039
0.693HisMet: 0.693 ± 0.023
0.822HisAsn: 0.822 ± 0.023
1.246HisPro: 1.246 ± 0.028
0.949HisGln: 0.949 ± 0.023
1.185HisArg: 1.185 ± 0.024
1.388HisSer: 1.388 ± 0.028
1.338HisThr: 1.338 ± 0.03
1.517HisVal: 1.517 ± 0.03
0.354HisTrp: 0.354 ± 0.013
0.922HisTyr: 0.922 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
5.605IleAla: 5.605 ± 0.063
0.582IleCys: 0.582 ± 0.016
3.286IleAsp: 3.286 ± 0.043
4.179IleGlu: 4.179 ± 0.051
2.247IlePhe: 2.247 ± 0.042
4.932IleGly: 4.932 ± 0.061
1.608IleHis: 1.608 ± 0.027
4.074IleIle: 4.074 ± 0.059
2.682IleLys: 2.682 ± 0.04
5.737IleLeu: 5.737 ± 0.067
1.739IleMet: 1.739 ± 0.032
2.207IleAsn: 2.207 ± 0.041
3.126IlePro: 3.126 ± 0.049
2.959IleGln: 2.959 ± 0.039
3.843IleArg: 3.843 ± 0.05
4.464IleSer: 4.464 ± 0.051
4.032IleThr: 4.032 ± 0.042
4.913IleVal: 4.913 ± 0.054
0.701IleTrp: 0.701 ± 0.017
2.062IleTyr: 2.062 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.861LysAla: 3.861 ± 0.057
0.256LysCys: 0.256 ± 0.011
2.731LysAsp: 2.731 ± 0.037
3.88LysGlu: 3.88 ± 0.054
1.277LysPhe: 1.277 ± 0.028
3.384LysGly: 3.384 ± 0.039
1.092LysHis: 1.092 ± 0.025
2.76LysIle: 2.76 ± 0.04
2.734LysLys: 2.734 ± 0.052
5.033LysLeu: 5.033 ± 0.052
1.348LysMet: 1.348 ± 0.028
1.762LysAsn: 1.762 ± 0.033
1.92LysPro: 1.92 ± 0.033
2.425LysGln: 2.425 ± 0.038
2.674LysArg: 2.674 ± 0.036
2.748LysSer: 2.748 ± 0.042
2.457LysThr: 2.457 ± 0.039
3.338LysVal: 3.338 ± 0.051
0.625LysTrp: 0.625 ± 0.017
1.699LysTyr: 1.699 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
8.094LeuAla: 8.094 ± 0.071
0.861LeuCys: 0.861 ± 0.023
5.238LeuAsp: 5.238 ± 0.051
6.913LeuGlu: 6.913 ± 0.073
3.963LeuPhe: 3.963 ± 0.064
6.796LeuGly: 6.796 ± 0.064
2.351LeuHis: 2.351 ± 0.042
6.268LeuIle: 6.268 ± 0.07
4.88LeuLys: 4.88 ± 0.057
11.29LeuLeu: 11.29 ± 0.122
2.753LeuMet: 2.753 ± 0.041
3.856LeuAsn: 3.856 ± 0.052
4.43LeuPro: 4.43 ± 0.054
4.6LeuGln: 4.6 ± 0.062
5.341LeuArg: 5.341 ± 0.058
7.129LeuSer: 7.129 ± 0.068
5.715LeuThr: 5.715 ± 0.05
6.368LeuVal: 6.368 ± 0.064
1.049LeuTrp: 1.049 ± 0.027
3.293LeuTyr: 3.293 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
2.211MetAla: 2.211 ± 0.035
0.176MetCys: 0.176 ± 0.01
1.622MetAsp: 1.622 ± 0.029
2.018MetGlu: 2.018 ± 0.033
0.934MetPhe: 0.934 ± 0.022
1.814MetGly: 1.814 ± 0.038
0.55MetHis: 0.55 ± 0.016
1.957MetIle: 1.957 ± 0.033
1.984MetLys: 1.984 ± 0.034
3.245MetLeu: 3.245 ± 0.051
0.9MetMet: 0.9 ± 0.025
1.602MetAsn: 1.602 ± 0.032
1.101MetPro: 1.101 ± 0.027
1.149MetGln: 1.149 ± 0.022
1.314MetArg: 1.314 ± 0.025
2.05MetSer: 2.05 ± 0.033
1.764MetThr: 1.764 ± 0.033
1.745MetVal: 1.745 ± 0.035
0.235MetTrp: 0.235 ± 0.012
0.862MetTyr: 0.862 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.718AsnAla: 2.718 ± 0.043
0.245AsnCys: 0.245 ± 0.011
1.808AsnAsp: 1.808 ± 0.036
2.579AsnGlu: 2.579 ± 0.043
1.211AsnPhe: 1.211 ± 0.027
3.056AsnGly: 3.056 ± 0.055
0.825AsnHis: 0.825 ± 0.021
2.332AsnIle: 2.332 ± 0.036
1.854AsnLys: 1.854 ± 0.034
3.196AsnLeu: 3.196 ± 0.04
1.099AsnMet: 1.099 ± 0.027
1.573AsnAsn: 1.573 ± 0.035
1.989AsnPro: 1.989 ± 0.037
1.568AsnGln: 1.568 ± 0.026
2.027AsnArg: 2.027 ± 0.035
2.253AsnSer: 2.253 ± 0.04
2.166AsnThr: 2.166 ± 0.039
2.568AsnVal: 2.568 ± 0.04
0.491AsnTrp: 0.491 ± 0.019
1.336AsnTyr: 1.336 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
3.35ProAla: 3.35 ± 0.047
0.258ProCys: 0.258 ± 0.012
2.541ProAsp: 2.541 ± 0.043
3.459ProGlu: 3.459 ± 0.046
1.71ProPhe: 1.71 ± 0.029
3.007ProGly: 3.007 ± 0.05
0.953ProHis: 0.953 ± 0.022
2.374ProIle: 2.374 ± 0.039
1.612ProLys: 1.612 ± 0.033
3.941ProLeu: 3.941 ± 0.049
0.991ProMet: 0.991 ± 0.023
1.456ProAsn: 1.456 ± 0.032
1.294ProPro: 1.294 ± 0.032
1.539ProGln: 1.539 ± 0.032
1.545ProArg: 1.545 ± 0.031
2.622ProSer: 2.622 ± 0.04
2.097ProThr: 2.097 ± 0.039
3.302ProVal: 3.302 ± 0.049
0.5ProTrp: 0.5 ± 0.017
1.584ProTyr: 1.584 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.981GlnAla: 3.981 ± 0.055
0.274GlnCys: 0.274 ± 0.013
2.096GlnAsp: 2.096 ± 0.039
3.36GlnGlu: 3.36 ± 0.047
1.536GlnPhe: 1.536 ± 0.03
3.094GlnGly: 3.094 ± 0.038
1.14GlnHis: 1.14 ± 0.027
2.601GlnIle: 2.601 ± 0.038
1.87GlnLys: 1.87 ± 0.028
4.916GlnLeu: 4.916 ± 0.065
1.292GlnMet: 1.292 ± 0.026
1.307GlnAsn: 1.307 ± 0.029
1.793GlnPro: 1.793 ± 0.034
2.726GlnGln: 2.726 ± 0.044
2.379GlnArg: 2.379 ± 0.038
2.565GlnSer: 2.565 ± 0.042
2.101GlnThr: 2.101 ± 0.04
3.058GlnVal: 3.058 ± 0.047
0.581GlnTrp: 0.581 ± 0.017
1.536GlnTyr: 1.536 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
3.402ArgAla: 3.402 ± 0.041
0.364ArgCys: 0.364 ± 0.014
2.519ArgAsp: 2.519 ± 0.039
3.866ArgGlu: 3.866 ± 0.046
2.029ArgPhe: 2.029 ± 0.036
3.088ArgGly: 3.088 ± 0.045
1.218ArgHis: 1.218 ± 0.029
3.75ArgIle: 3.75 ± 0.044
2.877ArgLys: 2.877 ± 0.044
5.708ArgLeu: 5.708 ± 0.065
1.834ArgMet: 1.834 ± 0.032
1.938ArgAsn: 1.938 ± 0.032
1.897ArgPro: 1.897 ± 0.035
2.446ArgGln: 2.446 ± 0.041
2.921ArgArg: 2.921 ± 0.048
3.352ArgSer: 3.352 ± 0.049
2.88ArgThr: 2.88 ± 0.045
3.353ArgVal: 3.353 ± 0.042
0.753ArgTrp: 0.753 ± 0.02
2.062ArgTyr: 2.062 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
4.897SerAla: 4.897 ± 0.049
0.454SerCys: 0.454 ± 0.017
3.041SerAsp: 3.041 ± 0.044
3.946SerGlu: 3.946 ± 0.052
2.94SerPhe: 2.94 ± 0.044
5.347SerGly: 5.347 ± 0.063
1.345SerHis: 1.345 ± 0.026
4.365SerIle: 4.365 ± 0.05
3.053SerLys: 3.053 ± 0.043
6.346SerLeu: 6.346 ± 0.067
2.05SerMet: 2.05 ± 0.034
2.418SerAsn: 2.418 ± 0.045
2.458SerPro: 2.458 ± 0.04
2.361SerGln: 2.361 ± 0.037
3.329SerArg: 3.329 ± 0.046
4.997SerSer: 4.997 ± 0.07
3.583SerThr: 3.583 ± 0.056
4.483SerVal: 4.483 ± 0.053
0.9SerTrp: 0.9 ± 0.023
2.48SerTyr: 2.48 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
4.945ThrAla: 4.945 ± 0.064
0.359ThrCys: 0.359 ± 0.016
3.027ThrAsp: 3.027 ± 0.052
3.52ThrGlu: 3.52 ± 0.046
2.258ThrPhe: 2.258 ± 0.037
4.893ThrGly: 4.893 ± 0.13
1.116ThrHis: 1.116 ± 0.024
3.676ThrIle: 3.676 ± 0.05
2.311ThrLys: 2.311 ± 0.042
5.501ThrLeu: 5.501 ± 0.06
1.399ThrMet: 1.399 ± 0.025
2.012ThrAsn: 2.012 ± 0.039
2.678ThrPro: 2.678 ± 0.045
1.863ThrGln: 1.863 ± 0.03
2.486ThrArg: 2.486 ± 0.035
3.644ThrSer: 3.644 ± 0.053
3.104ThrThr: 3.104 ± 0.056
4.483ThrVal: 4.483 ± 0.073
0.671ThrTrp: 0.671 ± 0.019
1.912ThrTyr: 1.912 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
5.412ValAla: 5.412 ± 0.06
0.617ValCys: 0.617 ± 0.021
3.678ValAsp: 3.678 ± 0.053
4.531ValGlu: 4.531 ± 0.056
2.705ValPhe: 2.705 ± 0.046
4.456ValGly: 4.456 ± 0.051
1.631ValHis: 1.631 ± 0.03
4.806ValIle: 4.806 ± 0.056
3.382ValLys: 3.382 ± 0.046
7.331ValLeu: 7.331 ± 0.075
1.991ValMet: 1.991 ± 0.035
2.799ValAsn: 2.799 ± 0.044
2.976ValPro: 2.976 ± 0.044
3.001ValGln: 3.001 ± 0.041
3.592ValArg: 3.592 ± 0.051
4.736ValSer: 4.736 ± 0.053
4.487ValThr: 4.487 ± 0.068
4.901ValVal: 4.901 ± 0.063
0.789ValTrp: 0.789 ± 0.022
2.441ValTyr: 2.441 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.819TrpAla: 0.819 ± 0.023
0.117TrpCys: 0.117 ± 0.008
0.628TrpAsp: 0.628 ± 0.021
0.81TrpGlu: 0.81 ± 0.02
0.525TrpPhe: 0.525 ± 0.018
0.846TrpGly: 0.846 ± 0.024
0.261TrpHis: 0.261 ± 0.011
0.879TrpIle: 0.879 ± 0.023
0.678TrpLys: 0.678 ± 0.019
1.446TrpLeu: 1.446 ± 0.029
0.453TrpMet: 0.453 ± 0.014
0.648TrpAsn: 0.648 ± 0.018
0.371TrpPro: 0.371 ± 0.014
0.526TrpGln: 0.526 ± 0.019
0.656TrpArg: 0.656 ± 0.02
0.86TrpSer: 0.86 ± 0.022
0.741TrpThr: 0.741 ± 0.026
0.8TrpVal: 0.8 ± 0.02
0.183TrpTrp: 0.183 ± 0.011
0.441TrpTyr: 0.441 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.746TyrAla: 2.746 ± 0.036
0.324TyrCys: 0.324 ± 0.011
1.909TyrAsp: 1.909 ± 0.039
2.42TyrGlu: 2.42 ± 0.038
1.499TyrPhe: 1.499 ± 0.026
2.702TyrGly: 2.702 ± 0.038
0.804TyrHis: 0.804 ± 0.021
2.211TyrIle: 2.211 ± 0.038
1.604TyrLys: 1.604 ± 0.029
3.286TyrLeu: 3.286 ± 0.044
0.999TyrMet: 0.999 ± 0.027
1.427TyrAsn: 1.427 ± 0.03
1.548TyrPro: 1.548 ± 0.033
1.435TyrGln: 1.435 ± 0.027
2.117TyrArg: 2.117 ± 0.037
2.251TyrSer: 2.251 ± 0.036
2.121TyrThr: 2.121 ± 0.037
2.486TyrVal: 2.486 ± 0.035
0.472TyrTrp: 0.472 ± 0.016
1.429TyrTyr: 1.429 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6028 proteins (1882351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski