Amino acid dipepetide frequency for Synechococcus sp. (strain CC9311)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.56AlaAla: 10.56 ± 0.165	1.387AlaCys: 1.387 ± 0.049	5.139AlaAsp: 5.139 ± 0.089	5.886AlaGlu: 5.886 ± 0.09	3.193AlaPhe: 3.193 ± 0.083	7.563AlaGly: 7.563 ± 0.113	1.577AlaHis: 1.577 ± 0.048	5.277AlaIle: 5.277 ± 0.079	2.968AlaLys: 2.968 ± 0.075	12.446AlaLeu: 12.446 ± 0.166	2.655AlaMet: 2.655 ± 0.062	2.695AlaAsn: 2.695 ± 0.067	4.248AlaPro: 4.248 ± 0.091	3.678AlaGln: 3.678 ± 0.081	5.293AlaArg: 5.293 ± 0.089	6.518AlaSer: 6.518 ± 0.088	4.49AlaThr: 4.49 ± 0.079	7.058AlaVal: 7.058 ± 0.105	1.484AlaTrp: 1.484 ± 0.044	1.859AlaTyr: 1.859 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.923CysAla: 0.923 ± 0.033	0.328CysCys: 0.328 ± 0.025	0.735CysAsp: 0.735 ± 0.029	0.613CysGlu: 0.613 ± 0.031	0.578CysPhe: 0.578 ± 0.031	1.181CysGly: 1.181 ± 0.044	0.355CysHis: 0.355 ± 0.023	0.614CysIle: 0.614 ± 0.027	0.405CysLys: 0.405 ± 0.025	1.602CysLeu: 1.602 ± 0.043	0.255CysMet: 0.255 ± 0.018	0.355CysAsn: 0.355 ± 0.02	0.71CysPro: 0.71 ± 0.035	0.469CysGln: 0.469 ± 0.022	1.014CysArg: 1.014 ± 0.034	1.075CysSer: 1.075 ± 0.039	0.543CysThr: 0.543 ± 0.029	0.753CysVal: 0.753 ± 0.034	0.296CysTrp: 0.296 ± 0.02	0.233CysTyr: 0.233 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.838AspAla: 4.838 ± 0.09	0.712AspCys: 0.712 ± 0.031	2.72AspAsp: 2.72 ± 0.07	2.889AspGlu: 2.889 ± 0.08	1.755AspPhe: 1.755 ± 0.054	4.443AspGly: 4.443 ± 0.079	1.293AspHis: 1.293 ± 0.048	1.823AspIle: 1.823 ± 0.053	1.252AspLys: 1.252 ± 0.046	7.266AspLeu: 7.266 ± 0.125	0.9AspMet: 0.9 ± 0.037	1.399AspAsn: 1.399 ± 0.05	3.674AspPro: 3.674 ± 0.075	3.205AspGln: 3.205 ± 0.072	3.74AspArg: 3.74 ± 0.073	3.199AspSer: 3.199 ± 0.075	1.994AspThr: 1.994 ± 0.061	3.496AspVal: 3.496 ± 0.067	1.035AspTrp: 1.035 ± 0.037	1.176AspTyr: 1.176 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	6.565GluAla: 6.565 ± 0.11	0.494GluCys: 0.494 ± 0.024	2.535GluAsp: 2.535 ± 0.061	3.501GluGlu: 3.501 ± 0.082	1.473GluPhe: 1.473 ± 0.046	4.408GluGly: 4.408 ± 0.083	1.216GluHis: 1.216 ± 0.043	2.809GluIle: 2.809 ± 0.072	1.879GluLys: 1.879 ± 0.056	7.581GluLeu: 7.581 ± 0.114	1.197GluMet: 1.197 ± 0.042	1.419GluAsn: 1.419 ± 0.05	2.797GluPro: 2.797 ± 0.079	3.534GluGln: 3.534 ± 0.071	4.765GluArg: 4.765 ± 0.082	3.522GluSer: 3.522 ± 0.074	2.829GluThr: 2.829 ± 0.064	3.785GluVal: 3.785 ± 0.079	0.792GluTrp: 0.792 ± 0.032	0.748GluTyr: 0.748 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	2.995PheAla: 2.995 ± 0.066	0.53PheCys: 0.53 ± 0.027	2.113PheAsp: 2.113 ± 0.058	1.944PheGlu: 1.944 ± 0.059	1.285PhePhe: 1.285 ± 0.049	3.044PheGly: 3.044 ± 0.071	0.692PheHis: 0.692 ± 0.029	1.479PheIle: 1.479 ± 0.047	1.058PheLys: 1.058 ± 0.036	3.481PheLeu: 3.481 ± 0.085	0.652PheMet: 0.652 ± 0.029	1.358PheAsn: 1.358 ± 0.05	1.419PhePro: 1.419 ± 0.046	1.469PheGln: 1.469 ± 0.048	1.962PheArg: 1.962 ± 0.058	2.695PheSer: 2.695 ± 0.066	1.743PheThr: 1.743 ± 0.051	2.114PheVal: 2.114 ± 0.062	0.614PheTrp: 0.614 ± 0.03	0.796PheTyr: 0.796 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.514GlyAla: 6.514 ± 0.106	1.334GlyCys: 1.334 ± 0.041	4.196GlyAsp: 4.196 ± 0.082	4.221GlyGlu: 4.221 ± 0.081	3.259GlyPhe: 3.259 ± 0.066	6.618GlyGly: 6.618 ± 0.145	1.743GlyHis: 1.743 ± 0.056	4.222GlyIle: 4.222 ± 0.074	2.582GlyLys: 2.582 ± 0.075	10.217GlyLeu: 10.217 ± 0.136	1.961GlyMet: 1.961 ± 0.055	2.264GlyAsn: 2.264 ± 0.064	3.041GlyPro: 3.041 ± 0.065	3.383GlyGln: 3.383 ± 0.069	5.196GlyArg: 5.196 ± 0.089	5.487GlySer: 5.487 ± 0.092	3.979GlyThr: 3.979 ± 0.074	5.861GlyVal: 5.861 ± 0.091	1.679GlyTrp: 1.679 ± 0.056	1.875GlyTyr: 1.875 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.703HisAla: 1.703 ± 0.051	0.388HisCys: 0.388 ± 0.022	1.047HisAsp: 1.047 ± 0.035	0.923HisGlu: 0.923 ± 0.037	0.775HisPhe: 0.775 ± 0.033	1.82HisGly: 1.82 ± 0.054	0.81HisHis: 0.81 ± 0.039	0.768HisIle: 0.768 ± 0.037	0.474HisLys: 0.474 ± 0.024	2.58HisLeu: 2.58 ± 0.066	0.272HisMet: 0.272 ± 0.02	0.61HisAsn: 0.61 ± 0.026	1.605HisPro: 1.605 ± 0.051	1.168HisGln: 1.168 ± 0.041	1.667HisArg: 1.667 ± 0.049	1.37HisSer: 1.37 ± 0.045	0.82HisThr: 0.82 ± 0.032	1.067HisVal: 1.067 ± 0.039	0.539HisTrp: 0.539 ± 0.026	0.488HisTyr: 0.488 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.263IleAla: 5.263 ± 0.087	0.711IleCys: 0.711 ± 0.033	3.156IleAsp: 3.156 ± 0.058	3.194IleGlu: 3.194 ± 0.073	1.317IlePhe: 1.317 ± 0.044	4.569IleGly: 4.569 ± 0.08	1.181IleHis: 1.181 ± 0.038	1.687IleIle: 1.687 ± 0.06	1.657IleLys: 1.657 ± 0.051	4.011IleLeu: 4.011 ± 0.082	0.516IleMet: 0.516 ± 0.032	1.99IleAsn: 1.99 ± 0.066	2.756IlePro: 2.756 ± 0.065	2.148IleGln: 2.148 ± 0.05	3.11IleArg: 3.11 ± 0.065	3.767IleSer: 3.767 ± 0.085	2.902IleThr: 2.902 ± 0.078	2.911IleVal: 2.911 ± 0.065	0.764IleTrp: 0.764 ± 0.039	0.977IleTyr: 0.977 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.634LysAla: 3.634 ± 0.074	0.276LysCys: 0.276 ± 0.02	1.738LysAsp: 1.738 ± 0.048	2.022LysGlu: 2.022 ± 0.057	0.688LysPhe: 0.688 ± 0.029	2.469LysGly: 2.469 ± 0.059	0.594LysHis: 0.594 ± 0.027	1.406LysIle: 1.406 ± 0.053	1.403LysLys: 1.403 ± 0.047	3.274LysLeu: 3.274 ± 0.079	0.559LysMet: 0.559 ± 0.03	0.953LysAsn: 0.953 ± 0.041	1.915LysPro: 1.915 ± 0.06	1.76LysGln: 1.76 ± 0.053	2.505LysArg: 2.505 ± 0.067	2.067LysSer: 2.067 ± 0.06	1.804LysThr: 1.804 ± 0.054	2.2LysVal: 2.2 ± 0.057	0.26LysTrp: 0.26 ± 0.019	0.547LysTyr: 0.547 ± 0.03	0.0LysXaa: 0.0 ± 0.0
Leu	11.982LeuAla: 11.982 ± 0.129	1.41LeuCys: 1.41 ± 0.043	6.339LeuAsp: 6.339 ± 0.111	7.929LeuGlu: 7.929 ± 0.107	3.618LeuPhe: 3.618 ± 0.083	9.056LeuGly: 9.056 ± 0.123	2.402LeuHis: 2.402 ± 0.073	6.573LeuIle: 6.573 ± 0.109	4.365LeuLys: 4.365 ± 0.085	15.062LeuLeu: 15.062 ± 0.211	2.947LeuMet: 2.947 ± 0.066	3.969LeuAsn: 3.969 ± 0.073	6.739LeuPro: 6.739 ± 0.108	5.828LeuGln: 5.828 ± 0.104	8.05LeuArg: 8.05 ± 0.117	8.739LeuSer: 8.739 ± 0.111	5.663LeuThr: 5.663 ± 0.087	8.663LeuVal: 8.663 ± 0.126	1.699LeuTrp: 1.699 ± 0.056	1.784LeuTyr: 1.784 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.839MetAla: 2.839 ± 0.06	0.153MetCys: 0.153 ± 0.015	1.067MetAsp: 1.067 ± 0.04	1.29MetGlu: 1.29 ± 0.041	0.509MetPhe: 0.509 ± 0.027	1.725MetGly: 1.725 ± 0.054	0.4MetHis: 0.4 ± 0.024	1.016MetIle: 1.016 ± 0.037	0.769MetLys: 0.769 ± 0.033	2.323MetLeu: 2.323 ± 0.061	0.328MetMet: 0.328 ± 0.023	0.775MetAsn: 0.775 ± 0.03	1.311MetPro: 1.311 ± 0.044	0.881MetGln: 0.881 ± 0.03	1.388MetArg: 1.388 ± 0.041	1.577MetSer: 1.577 ± 0.052	1.386MetThr: 1.386 ± 0.044	1.63MetVal: 1.63 ± 0.045	0.146MetTrp: 0.146 ± 0.013	0.239MetTyr: 0.239 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.867AsnAla: 2.867 ± 0.069	0.372AsnCys: 0.372 ± 0.023	1.666AsnAsp: 1.666 ± 0.06	1.492AsnGlu: 1.492 ± 0.055	0.998AsnPhe: 0.998 ± 0.042	2.687AsnGly: 2.687 ± 0.074	0.752AsnHis: 0.752 ± 0.032	1.234AsnIle: 1.234 ± 0.048	0.987AsnLys: 0.987 ± 0.041	3.482AsnLeu: 3.482 ± 0.07	0.486AsnMet: 0.486 ± 0.027	1.234AsnAsn: 1.234 ± 0.058	2.188AsnPro: 2.188 ± 0.056	1.681AsnGln: 1.681 ± 0.057	2.196AsnArg: 2.196 ± 0.055	2.168AsnSer: 2.168 ± 0.069	1.569AsnThr: 1.569 ± 0.055	1.741AsnVal: 1.741 ± 0.046	0.581AsnTrp: 0.581 ± 0.028	0.684AsnTyr: 0.684 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	4.735ProAla: 4.735 ± 0.091	0.582ProCys: 0.582 ± 0.028	3.189ProAsp: 3.189 ± 0.066	3.712ProGlu: 3.712 ± 0.066	1.948ProPhe: 1.948 ± 0.047	4.067ProGly: 4.067 ± 0.081	0.949ProHis: 0.949 ± 0.031	2.598ProIle: 2.598 ± 0.055	1.618ProLys: 1.618 ± 0.054	6.558ProLeu: 6.558 ± 0.111	1.222ProMet: 1.222 ± 0.043	1.59ProAsn: 1.59 ± 0.045	2.412ProPro: 2.412 ± 0.076	2.059ProGln: 2.059 ± 0.056	2.623ProArg: 2.623 ± 0.061	4.04ProSer: 4.04 ± 0.074	2.615ProThr: 2.615 ± 0.069	3.892ProVal: 3.892 ± 0.073	0.978ProTrp: 0.978 ± 0.036	1.011ProTyr: 1.011 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	4.901GlnAla: 4.901 ± 0.096	0.47GlnCys: 0.47 ± 0.025	1.981GlnAsp: 1.981 ± 0.052	2.697GlnGlu: 2.697 ± 0.066	1.286GlnPhe: 1.286 ± 0.047	3.306GlnGly: 3.306 ± 0.061	1.002GlnHis: 1.002 ± 0.04	2.105GlnIle: 2.105 ± 0.049	1.491GlnLys: 1.491 ± 0.05	6.303GlnLeu: 6.303 ± 0.111	0.89GlnMet: 0.89 ± 0.035	1.163GlnAsn: 1.163 ± 0.052	2.64GlnPro: 2.64 ± 0.059	3.001GlnGln: 3.001 ± 0.083	4.03GlnArg: 4.03 ± 0.083	2.989GlnSer: 2.989 ± 0.057	2.065GlnThr: 2.065 ± 0.055	3.142GlnVal: 3.142 ± 0.068	0.816GlnTrp: 0.816 ± 0.037	0.583GlnTyr: 0.583 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	4.888ArgAla: 4.888 ± 0.089	1.059ArgCys: 1.059 ± 0.038	3.452ArgAsp: 3.452 ± 0.068	3.455ArgGlu: 3.455 ± 0.072	2.841ArgPhe: 2.841 ± 0.077	4.304ArgGly: 4.304 ± 0.083	1.578ArgHis: 1.578 ± 0.049	3.292ArgIle: 3.292 ± 0.062	2.014ArgLys: 2.014 ± 0.068	9.0ArgLeu: 9.0 ± 0.139	1.586ArgMet: 1.586 ± 0.051	2.017ArgAsn: 2.017 ± 0.048	3.213ArgPro: 3.213 ± 0.074	3.544ArgGln: 3.544 ± 0.082	5.417ArgArg: 5.417 ± 0.118	4.9ArgSer: 4.9 ± 0.086	2.846ArgThr: 2.846 ± 0.061	4.08ArgVal: 4.08 ± 0.085	1.675ArgTrp: 1.675 ± 0.055	1.62ArgTyr: 1.62 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	6.364SerAla: 6.364 ± 0.112	0.963SerCys: 0.963 ± 0.035	3.827SerAsp: 3.827 ± 0.085	3.892SerGlu: 3.892 ± 0.073	2.538SerPhe: 2.538 ± 0.07	6.096SerGly: 6.096 ± 0.106	1.466SerHis: 1.466 ± 0.049	3.538SerIle: 3.538 ± 0.085	2.299SerLys: 2.299 ± 0.071	8.274SerLeu: 8.274 ± 0.113	1.787SerMet: 1.787 ± 0.046	2.4SerAsn: 2.4 ± 0.067	3.315SerPro: 3.315 ± 0.071	2.761SerGln: 2.761 ± 0.063	4.408SerArg: 4.408 ± 0.098	5.57SerSer: 5.57 ± 0.118	3.749SerThr: 3.749 ± 0.076	4.508SerVal: 4.508 ± 0.088	1.306SerTrp: 1.306 ± 0.045	1.35SerTyr: 1.35 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.923ThrAla: 4.923 ± 0.082	0.549ThrCys: 0.549 ± 0.028	2.373ThrAsp: 2.373 ± 0.066	2.418ThrGlu: 2.418 ± 0.066	1.65ThrPhe: 1.65 ± 0.048	4.153ThrGly: 4.153 ± 0.087	0.897ThrHis: 0.897 ± 0.035	2.583ThrIle: 2.583 ± 0.064	1.479ThrLys: 1.479 ± 0.054	5.762ThrLeu: 5.762 ± 0.09	1.026ThrMet: 1.026 ± 0.036	1.499ThrAsn: 1.499 ± 0.052	3.257ThrPro: 3.257 ± 0.071	1.824ThrGln: 1.824 ± 0.059	2.657ThrArg: 2.657 ± 0.068	3.559ThrSer: 3.559 ± 0.076	2.935ThrThr: 2.935 ± 0.067	3.522ThrVal: 3.522 ± 0.063	0.692ThrTrp: 0.692 ± 0.032	1.088ThrTyr: 1.088 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	6.647ValAla: 6.647 ± 0.108	0.771ValCys: 0.771 ± 0.035	3.795ValAsp: 3.795 ± 0.062	4.106ValGlu: 4.106 ± 0.084	2.419ValPhe: 2.419 ± 0.061	5.042ValGly: 5.042 ± 0.082	1.26ValHis: 1.26 ± 0.046	3.884ValIle: 3.884 ± 0.062	2.219ValLys: 2.219 ± 0.055	8.828ValLeu: 8.828 ± 0.122	1.733ValMet: 1.733 ± 0.049	2.241ValAsn: 2.241 ± 0.063	3.218ValPro: 3.218 ± 0.073	2.672ValGln: 2.672 ± 0.061	3.979ValArg: 3.979 ± 0.076	4.563ValSer: 4.563 ± 0.075	3.373ValThr: 3.373 ± 0.077	6.172ValVal: 6.172 ± 0.114	0.874ValTrp: 0.874 ± 0.032	1.084ValTyr: 1.084 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.194TrpAla: 1.194 ± 0.05	0.243TrpCys: 0.243 ± 0.019	0.737TrpAsp: 0.737 ± 0.03	0.708TrpGlu: 0.708 ± 0.034	0.638TrpPhe: 0.638 ± 0.03	1.135TrpGly: 1.135 ± 0.042	0.38TrpHis: 0.38 ± 0.025	0.958TrpIle: 0.958 ± 0.036	0.577TrpLys: 0.577 ± 0.028	2.523TrpLeu: 2.523 ± 0.066	0.485TrpMet: 0.485 ± 0.028	0.578TrpAsn: 0.578 ± 0.029	0.913TrpPro: 0.913 ± 0.038	0.946TrpGln: 0.946 ± 0.035	1.278TrpArg: 1.278 ± 0.042	1.246TrpSer: 1.246 ± 0.047	0.785TrpThr: 0.785 ± 0.032	1.003TrpVal: 1.003 ± 0.035	0.416TrpTrp: 0.416 ± 0.029	0.27TrpTyr: 0.27 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.636TyrAla: 1.636 ± 0.051	0.304TyrCys: 0.304 ± 0.02	1.004TyrAsp: 1.004 ± 0.039	0.958TyrGlu: 0.958 ± 0.04	0.675TyrPhe: 0.675 ± 0.031	1.949TyrGly: 1.949 ± 0.052	0.349TyrHis: 0.349 ± 0.024	0.72TyrIle: 0.72 ± 0.035	0.581TyrLys: 0.581 ± 0.03	2.243TyrLeu: 2.243 ± 0.056	0.294TyrMet: 0.294 ± 0.02	0.559TyrAsn: 0.559 ± 0.026	1.03TyrPro: 1.03 ± 0.036	0.85TyrGln: 0.85 ± 0.037	1.5TyrArg: 1.5 ± 0.053	1.357TyrSer: 1.357 ± 0.041	0.756TyrThr: 0.756 ± 0.032	1.281TyrVal: 1.281 ± 0.039	0.365TyrTrp: 0.365 ± 0.025	0.46TyrTyr: 0.46 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2882 proteins (752638 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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