Amino acid dipepetide frequency for Salmonella phage SEN4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.297AlaAla: 11.297 ± 1.85
0.772AlaCys: 0.772 ± 0.314
7.821AlaAsp: 7.821 ± 1.146
5.697AlaGlu: 5.697 ± 0.762
3.572AlaPhe: 3.572 ± 0.484
7.917AlaGly: 7.917 ± 0.927
1.352AlaHis: 1.352 ± 0.319
3.862AlaIle: 3.862 ± 0.563
5.6AlaLys: 5.6 ± 0.88
7.531AlaLeu: 7.531 ± 0.807
2.317AlaMet: 2.317 ± 0.472
3.862AlaAsn: 3.862 ± 0.57
4.538AlaPro: 4.538 ± 0.681
4.441AlaGln: 4.441 ± 0.525
6.373AlaArg: 6.373 ± 0.9
6.662AlaSer: 6.662 ± 0.827
5.793AlaThr: 5.793 ± 0.725
8.69AlaVal: 8.69 ± 0.749
1.641AlaTrp: 1.641 ± 0.42
3.669AlaTyr: 3.669 ± 0.521
0.0AlaXaa: 0.0 ± 0.0
Cys
1.062CysAla: 1.062 ± 0.427
0.29CysCys: 0.29 ± 0.216
0.772CysAsp: 0.772 ± 0.388
0.772CysGlu: 0.772 ± 0.289
0.193CysPhe: 0.193 ± 0.142
1.738CysGly: 1.738 ± 0.416
0.29CysHis: 0.29 ± 0.173
0.097CysIle: 0.097 ± 0.097
0.483CysLys: 0.483 ± 0.218
0.676CysLeu: 0.676 ± 0.224
0.29CysMet: 0.29 ± 0.153
0.772CysAsn: 0.772 ± 0.264
0.869CysPro: 0.869 ± 0.297
0.483CysGln: 0.483 ± 0.225
0.579CysArg: 0.579 ± 0.242
0.386CysSer: 0.386 ± 0.184
0.869CysThr: 0.869 ± 0.22
0.579CysVal: 0.579 ± 0.196
0.29CysTrp: 0.29 ± 0.178
0.483CysTyr: 0.483 ± 0.294
0.0CysXaa: 0.0 ± 0.0
Asp
7.338AspAla: 7.338 ± 0.907
0.869AspCys: 0.869 ± 0.28
3.283AspAsp: 3.283 ± 0.404
3.669AspGlu: 3.669 ± 0.53
2.221AspPhe: 2.221 ± 0.451
5.6AspGly: 5.6 ± 0.801
1.062AspHis: 1.062 ± 0.289
3.669AspIle: 3.669 ± 0.586
2.414AspLys: 2.414 ± 0.421
5.214AspLeu: 5.214 ± 0.727
0.676AspMet: 0.676 ± 0.281
2.8AspAsn: 2.8 ± 0.533
2.8AspPro: 2.8 ± 0.429
2.028AspGln: 2.028 ± 0.395
3.379AspArg: 3.379 ± 0.527
3.283AspSer: 3.283 ± 0.567
4.828AspThr: 4.828 ± 0.912
2.993AspVal: 2.993 ± 0.53
0.772AspTrp: 0.772 ± 0.307
2.51AspTyr: 2.51 ± 0.445
0.0AspXaa: 0.0 ± 0.0
Glu
4.635GluAla: 4.635 ± 0.631
0.869GluCys: 0.869 ± 0.421
2.703GluAsp: 2.703 ± 0.515
2.993GluGlu: 2.993 ± 0.637
2.028GluPhe: 2.028 ± 0.368
4.055GluGly: 4.055 ± 0.479
0.966GluHis: 0.966 ± 0.282
4.828GluIle: 4.828 ± 0.808
3.379GluLys: 3.379 ± 0.601
5.793GluLeu: 5.793 ± 0.916
1.931GluMet: 1.931 ± 0.429
2.221GluAsn: 2.221 ± 0.339
2.414GluPro: 2.414 ± 0.467
3.186GluGln: 3.186 ± 0.519
3.09GluArg: 3.09 ± 0.683
3.186GluSer: 3.186 ± 0.592
4.441GluThr: 4.441 ± 0.627
2.607GluVal: 2.607 ± 0.489
0.966GluTrp: 0.966 ± 0.271
1.641GluTyr: 1.641 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
3.283PheAla: 3.283 ± 0.47
0.483PheCys: 0.483 ± 0.209
2.51PheAsp: 2.51 ± 0.44
2.028PheGlu: 2.028 ± 0.429
1.641PhePhe: 1.641 ± 0.396
2.124PheGly: 2.124 ± 0.56
0.193PheHis: 0.193 ± 0.15
2.8PheIle: 2.8 ± 0.574
2.124PheLys: 2.124 ± 0.585
1.931PheLeu: 1.931 ± 0.46
1.159PheMet: 1.159 ± 0.326
1.931PheAsn: 1.931 ± 0.462
1.352PhePro: 1.352 ± 0.366
1.062PheGln: 1.062 ± 0.309
1.545PheArg: 1.545 ± 0.425
2.703PheSer: 2.703 ± 0.527
2.414PheThr: 2.414 ± 0.467
1.545PheVal: 1.545 ± 0.426
0.772PheTrp: 0.772 ± 0.313
0.772PheTyr: 0.772 ± 0.271
0.0PheXaa: 0.0 ± 0.0
Gly
6.952GlyAla: 6.952 ± 0.759
1.159GlyCys: 1.159 ± 0.332
5.6GlyAsp: 5.6 ± 0.846
4.248GlyGlu: 4.248 ± 0.86
3.766GlyPhe: 3.766 ± 0.703
5.697GlyGly: 5.697 ± 0.77
1.352GlyHis: 1.352 ± 0.398
3.379GlyIle: 3.379 ± 0.538
4.152GlyLys: 4.152 ± 0.64
5.31GlyLeu: 5.31 ± 0.775
2.414GlyMet: 2.414 ± 0.652
2.8GlyAsn: 2.8 ± 0.492
2.221GlyPro: 2.221 ± 0.438
2.703GlyGln: 2.703 ± 0.503
4.248GlyArg: 4.248 ± 0.755
4.441GlySer: 4.441 ± 0.741
3.283GlyThr: 3.283 ± 0.565
5.214GlyVal: 5.214 ± 0.705
1.738GlyTrp: 1.738 ± 0.42
2.221GlyTyr: 2.221 ± 0.376
0.0GlyXaa: 0.0 ± 0.0
His
2.028HisAla: 2.028 ± 0.408
0.29HisCys: 0.29 ± 0.212
1.159HisAsp: 1.159 ± 0.41
1.255HisGlu: 1.255 ± 0.493
0.772HisPhe: 0.772 ± 0.245
0.869HisGly: 0.869 ± 0.226
0.483HisHis: 0.483 ± 0.204
0.966HisIle: 0.966 ± 0.291
0.579HisLys: 0.579 ± 0.324
1.255HisLeu: 1.255 ± 0.426
0.29HisMet: 0.29 ± 0.159
0.483HisAsn: 0.483 ± 0.173
0.772HisPro: 0.772 ± 0.261
0.676HisGln: 0.676 ± 0.277
1.159HisArg: 1.159 ± 0.371
1.545HisSer: 1.545 ± 0.579
0.869HisThr: 0.869 ± 0.325
1.255HisVal: 1.255 ± 0.368
0.579HisTrp: 0.579 ± 0.304
0.29HisTyr: 0.29 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
5.697IleAla: 5.697 ± 0.809
0.579IleCys: 0.579 ± 0.272
4.152IleAsp: 4.152 ± 0.701
3.572IleGlu: 3.572 ± 0.658
1.545IlePhe: 1.545 ± 0.409
4.731IleGly: 4.731 ± 0.823
0.386IleHis: 0.386 ± 0.156
1.835IleIle: 1.835 ± 0.555
2.897IleLys: 2.897 ± 0.5
2.51IleLeu: 2.51 ± 0.498
1.062IleMet: 1.062 ± 0.33
2.607IleAsn: 2.607 ± 0.556
1.738IlePro: 1.738 ± 0.468
1.835IleGln: 1.835 ± 0.436
3.09IleArg: 3.09 ± 0.498
4.538IleSer: 4.538 ± 0.595
4.731IleThr: 4.731 ± 0.847
2.51IleVal: 2.51 ± 0.429
0.579IleTrp: 0.579 ± 0.29
1.931IleTyr: 1.931 ± 0.402
0.0IleXaa: 0.0 ± 0.0
Lys
5.407LysAla: 5.407 ± 0.9
0.193LysCys: 0.193 ± 0.131
2.703LysAsp: 2.703 ± 0.506
2.993LysGlu: 2.993 ± 0.537
1.448LysPhe: 1.448 ± 0.529
3.572LysGly: 3.572 ± 0.639
0.772LysHis: 0.772 ± 0.283
2.703LysIle: 2.703 ± 0.579
5.021LysLys: 5.021 ± 0.781
4.731LysLeu: 4.731 ± 0.626
0.869LysMet: 0.869 ± 0.246
2.414LysAsn: 2.414 ± 0.474
2.607LysPro: 2.607 ± 0.453
2.028LysGln: 2.028 ± 0.572
3.283LysArg: 3.283 ± 0.672
2.8LysSer: 2.8 ± 0.56
3.476LysThr: 3.476 ± 0.657
2.703LysVal: 2.703 ± 0.538
0.772LysTrp: 0.772 ± 0.3
1.738LysTyr: 1.738 ± 0.39
0.0LysXaa: 0.0 ± 0.0
Leu
9.462LeuAla: 9.462 ± 1.004
0.966LeuCys: 0.966 ± 0.261
4.055LeuAsp: 4.055 ± 0.684
4.924LeuGlu: 4.924 ± 0.633
2.703LeuPhe: 2.703 ± 0.486
5.793LeuGly: 5.793 ± 0.786
1.352LeuHis: 1.352 ± 0.396
4.055LeuIle: 4.055 ± 0.54
4.731LeuLys: 4.731 ± 0.799
6.469LeuLeu: 6.469 ± 0.729
2.221LeuMet: 2.221 ± 0.358
3.379LeuAsn: 3.379 ± 0.505
4.538LeuPro: 4.538 ± 0.708
2.414LeuGln: 2.414 ± 0.51
6.179LeuArg: 6.179 ± 0.871
5.89LeuSer: 5.89 ± 0.755
5.986LeuThr: 5.986 ± 1.083
5.214LeuVal: 5.214 ± 0.801
0.869LeuTrp: 0.869 ± 0.326
2.51LeuTyr: 2.51 ± 0.552
0.0LeuXaa: 0.0 ± 0.0
Met
2.221MetAla: 2.221 ± 0.422
0.193MetCys: 0.193 ± 0.13
1.448MetAsp: 1.448 ± 0.341
1.159MetGlu: 1.159 ± 0.336
0.966MetPhe: 0.966 ± 0.293
0.869MetGly: 0.869 ± 0.277
0.193MetHis: 0.193 ± 0.154
1.931MetIle: 1.931 ± 0.441
1.448MetLys: 1.448 ± 0.456
2.028MetLeu: 2.028 ± 0.34
0.966MetMet: 0.966 ± 0.318
0.772MetAsn: 0.772 ± 0.25
1.255MetPro: 1.255 ± 0.334
0.966MetGln: 0.966 ± 0.312
1.545MetArg: 1.545 ± 0.366
1.931MetSer: 1.931 ± 0.404
1.835MetThr: 1.835 ± 0.388
1.448MetVal: 1.448 ± 0.342
0.097MetTrp: 0.097 ± 0.093
0.386MetTyr: 0.386 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
4.152AsnAla: 4.152 ± 0.732
0.772AsnCys: 0.772 ± 0.285
2.607AsnAsp: 2.607 ± 0.56
1.545AsnGlu: 1.545 ± 0.353
1.159AsnPhe: 1.159 ± 0.294
3.862AsnGly: 3.862 ± 0.566
0.966AsnHis: 0.966 ± 0.34
1.545AsnIle: 1.545 ± 0.311
2.8AsnLys: 2.8 ± 0.553
2.897AsnLeu: 2.897 ± 0.489
0.676AsnMet: 0.676 ± 0.301
1.545AsnAsn: 1.545 ± 0.482
2.414AsnPro: 2.414 ± 0.512
1.641AsnGln: 1.641 ± 0.496
3.476AsnArg: 3.476 ± 0.604
2.124AsnSer: 2.124 ± 0.489
1.352AsnThr: 1.352 ± 0.37
1.835AsnVal: 1.835 ± 0.458
0.483AsnTrp: 0.483 ± 0.212
1.352AsnTyr: 1.352 ± 0.327
0.0AsnXaa: 0.0 ± 0.0
Pro
4.924ProAla: 4.924 ± 0.695
0.579ProCys: 0.579 ± 0.238
3.283ProAsp: 3.283 ± 0.707
3.476ProGlu: 3.476 ± 0.533
2.124ProPhe: 2.124 ± 0.663
2.8ProGly: 2.8 ± 0.46
1.159ProHis: 1.159 ± 0.375
2.317ProIle: 2.317 ± 0.562
1.448ProLys: 1.448 ± 0.365
3.766ProLeu: 3.766 ± 0.631
0.869ProMet: 0.869 ± 0.29
0.869ProAsn: 0.869 ± 0.336
2.8ProPro: 2.8 ± 0.604
1.159ProGln: 1.159 ± 0.284
1.835ProArg: 1.835 ± 0.399
3.476ProSer: 3.476 ± 0.54
1.448ProThr: 1.448 ± 0.368
4.635ProVal: 4.635 ± 0.593
0.869ProTrp: 0.869 ± 0.261
1.352ProTyr: 1.352 ± 0.395
0.0ProXaa: 0.0 ± 0.0
Gln
5.021GlnAla: 5.021 ± 1.0
0.29GlnCys: 0.29 ± 0.142
1.545GlnAsp: 1.545 ± 0.282
1.931GlnGlu: 1.931 ± 0.465
1.159GlnPhe: 1.159 ± 0.352
1.352GlnGly: 1.352 ± 0.325
1.159GlnHis: 1.159 ± 0.394
2.703GlnIle: 2.703 ± 0.606
2.703GlnLys: 2.703 ± 0.622
5.504GlnLeu: 5.504 ± 0.798
0.676GlnMet: 0.676 ± 0.266
1.448GlnAsn: 1.448 ± 0.386
1.835GlnPro: 1.835 ± 0.392
3.186GlnGln: 3.186 ± 0.768
3.572GlnArg: 3.572 ± 0.683
1.931GlnSer: 1.931 ± 0.374
2.607GlnThr: 2.607 ± 0.6
2.897GlnVal: 2.897 ± 0.541
0.579GlnTrp: 0.579 ± 0.212
1.545GlnTyr: 1.545 ± 0.354
0.0GlnXaa: 0.0 ± 0.0
Arg
6.083ArgAla: 6.083 ± 0.902
0.386ArgCys: 0.386 ± 0.194
3.766ArgAsp: 3.766 ± 0.559
4.538ArgGlu: 4.538 ± 0.945
1.835ArgPhe: 1.835 ± 0.506
3.476ArgGly: 3.476 ± 0.711
1.931ArgHis: 1.931 ± 0.404
2.607ArgIle: 2.607 ± 0.626
2.703ArgLys: 2.703 ± 0.648
5.117ArgLeu: 5.117 ± 0.754
1.448ArgMet: 1.448 ± 0.324
2.028ArgAsn: 2.028 ± 0.449
1.738ArgPro: 1.738 ± 0.401
5.021ArgGln: 5.021 ± 0.767
5.6ArgArg: 5.6 ± 0.913
2.8ArgSer: 2.8 ± 0.484
3.186ArgThr: 3.186 ± 0.652
5.31ArgVal: 5.31 ± 0.7
1.448ArgTrp: 1.448 ± 0.317
2.414ArgTyr: 2.414 ± 0.558
0.0ArgXaa: 0.0 ± 0.0
Ser
6.179SerAla: 6.179 ± 0.841
0.869SerCys: 0.869 ± 0.244
3.379SerAsp: 3.379 ± 0.564
3.09SerGlu: 3.09 ± 0.549
2.028SerPhe: 2.028 ± 0.444
6.083SerGly: 6.083 ± 0.803
1.448SerHis: 1.448 ± 0.438
2.897SerIle: 2.897 ± 0.577
2.414SerLys: 2.414 ± 0.437
6.566SerLeu: 6.566 ± 0.761
1.545SerMet: 1.545 ± 0.432
2.221SerAsn: 2.221 ± 0.642
2.51SerPro: 2.51 ± 0.384
2.414SerGln: 2.414 ± 0.379
3.766SerArg: 3.766 ± 0.561
4.055SerSer: 4.055 ± 0.789
3.283SerThr: 3.283 ± 0.608
4.538SerVal: 4.538 ± 0.564
0.676SerTrp: 0.676 ± 0.236
1.835SerTyr: 1.835 ± 0.467
0.0SerXaa: 0.0 ± 0.0
Thr
6.759ThrAla: 6.759 ± 0.887
0.386ThrCys: 0.386 ± 0.207
3.669ThrAsp: 3.669 ± 0.727
3.669ThrGlu: 3.669 ± 0.681
1.835ThrPhe: 1.835 ± 0.437
5.697ThrGly: 5.697 ± 0.69
0.676ThrHis: 0.676 ± 0.256
3.572ThrIle: 3.572 ± 0.715
2.414ThrLys: 2.414 ± 0.575
5.6ThrLeu: 5.6 ± 0.982
1.159ThrMet: 1.159 ± 0.365
1.641ThrAsn: 1.641 ± 0.459
3.379ThrPro: 3.379 ± 0.593
2.897ThrGln: 2.897 ± 0.568
4.152ThrArg: 4.152 ± 0.706
3.476ThrSer: 3.476 ± 0.526
4.538ThrThr: 4.538 ± 0.926
4.441ThrVal: 4.441 ± 0.722
0.869ThrTrp: 0.869 ± 0.319
1.641ThrTyr: 1.641 ± 0.492
0.0ThrXaa: 0.0 ± 0.0
Val
7.048ValAla: 7.048 ± 0.968
1.159ValCys: 1.159 ± 0.411
4.248ValAsp: 4.248 ± 0.622
4.538ValGlu: 4.538 ± 0.91
1.835ValPhe: 1.835 ± 0.434
3.379ValGly: 3.379 ± 0.561
0.966ValHis: 0.966 ± 0.268
3.186ValIle: 3.186 ± 0.721
3.09ValLys: 3.09 ± 0.538
5.31ValLeu: 5.31 ± 0.736
2.317ValMet: 2.317 ± 0.403
3.283ValAsn: 3.283 ± 0.533
3.09ValPro: 3.09 ± 0.654
3.186ValGln: 3.186 ± 0.504
2.897ValArg: 2.897 ± 0.396
3.379ValSer: 3.379 ± 0.689
4.924ValThr: 4.924 ± 0.56
3.862ValVal: 3.862 ± 0.68
0.966ValTrp: 0.966 ± 0.294
2.221ValTyr: 2.221 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
1.448TrpAla: 1.448 ± 0.45
0.29TrpCys: 0.29 ± 0.159
0.966TrpAsp: 0.966 ± 0.235
0.386TrpGlu: 0.386 ± 0.151
0.483TrpPhe: 0.483 ± 0.16
0.869TrpGly: 0.869 ± 0.286
0.29TrpHis: 0.29 ± 0.164
1.159TrpIle: 1.159 ± 0.443
0.483TrpLys: 0.483 ± 0.202
2.124TrpLeu: 2.124 ± 0.498
0.483TrpMet: 0.483 ± 0.239
0.676TrpAsn: 0.676 ± 0.3
0.966TrpPro: 0.966 ± 0.303
0.483TrpGln: 0.483 ± 0.199
1.641TrpArg: 1.641 ± 0.479
0.483TrpSer: 0.483 ± 0.216
0.772TrpThr: 0.772 ± 0.275
0.676TrpVal: 0.676 ± 0.232
0.483TrpTrp: 0.483 ± 0.214
0.579TrpTyr: 0.579 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.414TyrAla: 2.414 ± 0.512
0.579TyrCys: 0.579 ± 0.304
1.835TyrAsp: 1.835 ± 0.505
1.448TyrGlu: 1.448 ± 0.425
1.062TyrPhe: 1.062 ± 0.429
2.414TyrGly: 2.414 ± 0.495
0.579TyrHis: 0.579 ± 0.285
2.414TyrIle: 2.414 ± 0.483
1.352TyrLys: 1.352 ± 0.297
3.379TyrLeu: 3.379 ± 0.56
0.193TyrMet: 0.193 ± 0.135
1.448TyrAsn: 1.448 ± 0.428
1.352TyrPro: 1.352 ± 0.402
1.835TyrGln: 1.835 ± 0.522
2.028TyrArg: 2.028 ± 0.461
2.703TyrSer: 2.703 ± 0.46
1.835TyrThr: 1.835 ± 0.426
1.931TyrVal: 1.931 ± 0.584
0.29TyrTrp: 0.29 ± 0.147
0.869TyrTyr: 0.869 ± 0.299
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (10358 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski