Amino acid dipepetide frequency for BtRf-AlphaCoV/YN2012

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.684AlaAla: 4.684 ± 0.658
2.627AlaCys: 2.627 ± 0.876
2.856AlaAsp: 2.856 ± 0.55
1.599AlaGlu: 1.599 ± 0.92
4.227AlaPhe: 4.227 ± 1.29
3.655AlaGly: 3.655 ± 0.609
1.714AlaHis: 1.714 ± 0.563
4.227AlaIle: 4.227 ± 1.369
3.77AlaLys: 3.77 ± 0.43
6.626AlaLeu: 6.626 ± 1.167
1.942AlaMet: 1.942 ± 0.733
3.541AlaAsn: 3.541 ± 0.925
2.17AlaPro: 2.17 ± 1.342
1.942AlaGln: 1.942 ± 0.659
2.513AlaArg: 2.513 ± 1.032
3.77AlaSer: 3.77 ± 0.757
3.655AlaThr: 3.655 ± 0.244
6.74AlaVal: 6.74 ± 0.414
1.028AlaTrp: 1.028 ± 0.475
3.084AlaTyr: 3.084 ± 0.961
0.0AlaXaa: 0.0 ± 0.0
Cys
1.371CysAla: 1.371 ± 0.483
1.028CysCys: 1.028 ± 0.475
2.285CysAsp: 2.285 ± 0.737
1.028CysGlu: 1.028 ± 0.339
2.056CysPhe: 2.056 ± 0.341
3.427CysGly: 3.427 ± 0.731
0.457CysHis: 0.457 ± 0.35
1.714CysIle: 1.714 ± 0.348
2.627CysLys: 2.627 ± 0.86
2.513CysLeu: 2.513 ± 0.469
0.685CysMet: 0.685 ± 0.306
1.942CysAsn: 1.942 ± 0.666
1.028CysPro: 1.028 ± 0.392
0.457CysGln: 0.457 ± 0.161
1.142CysArg: 1.142 ± 0.573
1.599CysSer: 1.599 ± 0.521
2.056CysThr: 2.056 ± 0.653
2.513CysVal: 2.513 ± 0.929
0.571CysTrp: 0.571 ± 0.286
2.742CysTyr: 2.742 ± 0.936
0.0CysXaa: 0.0 ± 0.0
Asp
4.798AspAla: 4.798 ± 1.023
1.714AspCys: 1.714 ± 0.276
3.313AspAsp: 3.313 ± 1.213
2.285AspGlu: 2.285 ± 0.5
3.998AspPhe: 3.998 ± 1.258
5.483AspGly: 5.483 ± 1.211
1.142AspHis: 1.142 ± 0.573
3.541AspIle: 3.541 ± 0.861
3.199AspLys: 3.199 ± 0.991
4.227AspLeu: 4.227 ± 1.074
1.257AspMet: 1.257 ± 0.464
3.313AspAsn: 3.313 ± 0.975
1.485AspPro: 1.485 ± 0.574
1.142AspGln: 1.142 ± 0.263
1.485AspArg: 1.485 ± 0.336
3.884AspSer: 3.884 ± 0.258
3.084AspThr: 3.084 ± 1.202
5.369AspVal: 5.369 ± 1.605
0.8AspTrp: 0.8 ± 0.261
2.97AspTyr: 2.97 ± 0.568
0.0AspXaa: 0.0 ± 0.0
Glu
2.285GluAla: 2.285 ± 1.392
1.714GluCys: 1.714 ± 0.633
2.17GluAsp: 2.17 ± 0.425
1.599GluGlu: 1.599 ± 0.491
2.285GluPhe: 2.285 ± 0.634
2.285GluGly: 2.285 ± 0.634
1.257GluHis: 1.257 ± 0.494
2.627GluIle: 2.627 ± 0.897
1.942GluLys: 1.942 ± 0.614
3.313GluLeu: 3.313 ± 0.548
0.457GluMet: 0.457 ± 0.332
1.257GluAsn: 1.257 ± 0.345
1.599GluPro: 1.599 ± 0.508
1.028GluGln: 1.028 ± 1.201
1.828GluArg: 1.828 ± 0.579
1.714GluSer: 1.714 ± 0.56
2.17GluThr: 2.17 ± 1.254
2.742GluVal: 2.742 ± 0.332
1.028GluTrp: 1.028 ± 0.244
1.714GluTyr: 1.714 ± 0.568
0.0GluXaa: 0.0 ± 0.0
Phe
3.313PheAla: 3.313 ± 1.314
1.942PheCys: 1.942 ± 0.487
5.255PheAsp: 5.255 ± 1.621
2.627PheGlu: 2.627 ± 0.876
3.084PhePhe: 3.084 ± 1.043
4.912PheGly: 4.912 ± 0.933
0.457PheHis: 0.457 ± 0.161
2.742PheIle: 2.742 ± 0.575
3.084PheLys: 3.084 ± 0.898
4.341PheLeu: 4.341 ± 0.807
0.914PheMet: 0.914 ± 0.458
3.541PheAsn: 3.541 ± 0.81
0.685PhePro: 0.685 ± 0.637
1.599PheGln: 1.599 ± 1.525
1.942PheArg: 1.942 ± 0.411
5.141PheSer: 5.141 ± 1.024
2.97PheThr: 2.97 ± 0.362
6.397PheVal: 6.397 ± 0.902
1.028PheTrp: 1.028 ± 0.451
2.399PheTyr: 2.399 ± 0.523
0.0PheXaa: 0.0 ± 0.0
Gly
5.483GlyAla: 5.483 ± 0.869
2.399GlyCys: 2.399 ± 0.459
4.798GlyAsp: 4.798 ± 1.107
1.828GlyGlu: 1.828 ± 0.916
4.455GlyPhe: 4.455 ± 1.197
4.684GlyGly: 4.684 ± 0.839
1.371GlyHis: 1.371 ± 0.468
3.884GlyIle: 3.884 ± 1.798
3.998GlyLys: 3.998 ± 0.82
5.597GlyLeu: 5.597 ± 0.852
1.371GlyMet: 1.371 ± 0.435
2.513GlyAsn: 2.513 ± 0.799
2.17GlyPro: 2.17 ± 0.499
1.371GlyGln: 1.371 ± 0.53
2.285GlyArg: 2.285 ± 1.287
4.912GlySer: 4.912 ± 1.517
3.655GlyThr: 3.655 ± 0.557
7.654GlyVal: 7.654 ± 0.731
0.8GlyTrp: 0.8 ± 0.349
2.285GlyTyr: 2.285 ± 0.462
0.0GlyXaa: 0.0 ± 0.0
His
1.371HisAla: 1.371 ± 0.508
0.914HisCys: 0.914 ± 0.458
1.257HisAsp: 1.257 ± 0.464
0.571HisGlu: 0.571 ± 0.286
1.485HisPhe: 1.485 ± 0.61
1.485HisGly: 1.485 ± 0.398
0.343HisHis: 0.343 ± 0.404
0.914HisIle: 0.914 ± 0.322
1.485HisLys: 1.485 ± 0.508
1.714HisLeu: 1.714 ± 1.135
0.0HisMet: 0.0 ± 0.0
2.056HisAsn: 2.056 ± 0.653
0.343HisPro: 0.343 ± 0.172
0.457HisGln: 0.457 ± 0.161
0.8HisArg: 0.8 ± 0.452
1.257HisSer: 1.257 ± 0.398
0.571HisThr: 0.571 ± 0.286
2.285HisVal: 2.285 ± 0.822
0.114HisTrp: 0.114 ± 0.356
0.571HisTyr: 0.571 ± 0.648
0.0HisXaa: 0.0 ± 0.0
Ile
4.798IleAla: 4.798 ± 2.142
1.028IleCys: 1.028 ± 0.359
2.856IleAsp: 2.856 ± 0.828
2.056IleGlu: 2.056 ± 0.949
3.199IlePhe: 3.199 ± 0.91
3.427IleGly: 3.427 ± 1.101
0.343IleHis: 0.343 ± 0.379
3.199IleIle: 3.199 ± 2.659
3.427IleLys: 3.427 ± 1.095
4.569IleLeu: 4.569 ± 1.136
1.371IleMet: 1.371 ± 0.336
4.112IleAsn: 4.112 ± 0.759
2.17IlePro: 2.17 ± 0.496
1.828IleGln: 1.828 ± 0.692
1.942IleArg: 1.942 ± 1.09
4.227IleSer: 4.227 ± 1.273
3.884IleThr: 3.884 ± 1.176
5.369IleVal: 5.369 ± 0.589
0.343IleTrp: 0.343 ± 0.172
2.056IleTyr: 2.056 ± 1.266
0.0IleXaa: 0.0 ± 0.0
Lys
4.341LysAla: 4.341 ± 1.438
2.056LysCys: 2.056 ± 0.617
3.084LysAsp: 3.084 ± 0.788
1.599LysGlu: 1.599 ± 0.495
3.199LysPhe: 3.199 ± 1.258
3.084LysGly: 3.084 ± 0.659
2.056LysHis: 2.056 ± 0.622
2.513LysIle: 2.513 ± 0.712
1.942LysLys: 1.942 ± 0.369
6.283LysLeu: 6.283 ± 1.029
1.257LysMet: 1.257 ± 0.346
2.97LysAsn: 2.97 ± 0.664
3.884LysPro: 3.884 ± 1.018
2.627LysGln: 2.627 ± 1.173
2.17LysArg: 2.17 ± 0.888
2.856LysSer: 2.856 ± 0.585
2.97LysThr: 2.97 ± 0.664
4.341LysVal: 4.341 ± 1.383
0.571LysTrp: 0.571 ± 0.329
3.084LysTyr: 3.084 ± 0.898
0.0LysXaa: 0.0 ± 0.0
Leu
4.798LeuAla: 4.798 ± 1.247
2.513LeuCys: 2.513 ± 0.47
4.455LeuAsp: 4.455 ± 1.721
3.655LeuGlu: 3.655 ± 0.908
4.798LeuPhe: 4.798 ± 1.178
5.597LeuGly: 5.597 ± 0.908
2.513LeuHis: 2.513 ± 0.81
4.227LeuIle: 4.227 ± 0.68
6.169LeuLys: 6.169 ± 1.849
8.339LeuLeu: 8.339 ± 0.8
1.485LeuMet: 1.485 ± 1.104
4.798LeuAsn: 4.798 ± 0.886
3.084LeuPro: 3.084 ± 0.771
3.541LeuGln: 3.541 ± 0.783
3.084LeuArg: 3.084 ± 0.38
7.539LeuSer: 7.539 ± 1.455
4.569LeuThr: 4.569 ± 1.914
6.169LeuVal: 6.169 ± 2.424
1.257LeuTrp: 1.257 ± 0.973
4.455LeuTyr: 4.455 ± 2.508
0.0LeuXaa: 0.0 ± 0.0
Met
1.142MetAla: 1.142 ± 0.418
1.371MetCys: 1.371 ± 0.519
1.371MetAsp: 1.371 ± 0.285
1.028MetGlu: 1.028 ± 0.592
1.257MetPhe: 1.257 ± 1.552
0.914MetGly: 0.914 ± 0.322
0.685MetHis: 0.685 ± 0.218
0.914MetIle: 0.914 ± 0.344
0.914MetLys: 0.914 ± 0.459
2.627MetLeu: 2.627 ± 1.198
0.457MetMet: 0.457 ± 0.161
0.914MetAsn: 0.914 ± 0.308
1.371MetPro: 1.371 ± 0.335
0.571MetGln: 0.571 ± 0.464
0.571MetArg: 0.571 ± 0.286
1.485MetSer: 1.485 ± 0.36
1.714MetThr: 1.714 ± 0.41
1.028MetVal: 1.028 ± 0.244
0.114MetTrp: 0.114 ± 0.475
1.142MetTyr: 1.142 ± 0.332
0.0MetXaa: 0.0 ± 0.0
Asn
3.77AsnAla: 3.77 ± 1.592
2.513AsnCys: 2.513 ± 0.795
2.627AsnAsp: 2.627 ± 0.464
1.828AsnGlu: 1.828 ± 0.392
4.227AsnPhe: 4.227 ± 0.351
6.397AsnGly: 6.397 ± 0.854
0.685AsnHis: 0.685 ± 0.344
3.655AsnIle: 3.655 ± 1.013
2.856AsnLys: 2.856 ± 0.655
4.227AsnLeu: 4.227 ± 0.523
1.371AsnMet: 1.371 ± 0.724
2.513AsnAsn: 2.513 ± 1.078
1.257AsnPro: 1.257 ± 0.945
1.485AsnGln: 1.485 ± 0.826
1.599AsnArg: 1.599 ± 0.491
3.77AsnSer: 3.77 ± 0.76
3.541AsnThr: 3.541 ± 0.961
6.854AsnVal: 6.854 ± 1.486
0.571AsnTrp: 0.571 ± 1.267
2.285AsnTyr: 2.285 ± 0.624
0.0AsnXaa: 0.0 ± 0.0
Pro
2.285ProAla: 2.285 ± 0.807
0.685ProCys: 0.685 ± 0.346
0.8ProAsp: 0.8 ± 0.401
1.714ProGlu: 1.714 ± 0.398
2.056ProPhe: 2.056 ± 0.253
2.399ProGly: 2.399 ± 0.761
0.457ProHis: 0.457 ± 0.289
1.942ProIle: 1.942 ± 0.653
1.485ProLys: 1.485 ± 0.858
3.427ProLeu: 3.427 ± 0.62
0.457ProMet: 0.457 ± 0.403
1.828ProAsn: 1.828 ± 0.392
1.599ProPro: 1.599 ± 0.575
1.028ProGln: 1.028 ± 0.988
1.599ProArg: 1.599 ± 1.583
3.884ProSer: 3.884 ± 2.017
2.17ProThr: 2.17 ± 1.94
2.399ProVal: 2.399 ± 1.025
0.457ProTrp: 0.457 ± 0.229
1.371ProTyr: 1.371 ± 0.435
0.0ProXaa: 0.0 ± 0.0
Gln
1.942GlnAla: 1.942 ± 0.847
0.457GlnCys: 0.457 ± 0.161
1.485GlnAsp: 1.485 ± 0.404
0.571GlnGlu: 0.571 ± 0.361
1.371GlnPhe: 1.371 ± 0.377
2.056GlnGly: 2.056 ± 0.471
0.685GlnHis: 0.685 ± 0.316
1.485GlnIle: 1.485 ± 0.503
1.942GlnLys: 1.942 ± 0.764
3.77GlnLeu: 3.77 ± 1.084
0.8GlnMet: 0.8 ± 0.336
1.599GlnAsn: 1.599 ± 0.759
1.257GlnPro: 1.257 ± 0.731
1.142GlnGln: 1.142 ± 1.727
1.257GlnArg: 1.257 ± 0.292
2.856GlnSer: 2.856 ± 2.5
1.714GlnThr: 1.714 ± 0.722
1.485GlnVal: 1.485 ± 0.381
0.685GlnTrp: 0.685 ± 0.526
0.685GlnTyr: 0.685 ± 0.498
0.0GlnXaa: 0.0 ± 0.0
Arg
2.742ArgAla: 2.742 ± 0.351
1.599ArgCys: 1.599 ± 0.802
0.457ArgAsp: 0.457 ± 0.229
1.599ArgGlu: 1.599 ± 0.345
3.084ArgPhe: 3.084 ± 0.665
2.056ArgGly: 2.056 ± 0.87
0.685ArgHis: 0.685 ± 0.344
1.714ArgIle: 1.714 ± 1.313
1.942ArgLys: 1.942 ± 1.152
3.313ArgLeu: 3.313 ± 0.774
1.028ArgMet: 1.028 ± 0.315
2.856ArgAsn: 2.856 ± 1.78
0.8ArgPro: 0.8 ± 0.314
0.457ArgGln: 0.457 ± 0.229
1.257ArgArg: 1.257 ± 0.423
3.313ArgSer: 3.313 ± 2.476
1.828ArgThr: 1.828 ± 0.668
2.97ArgVal: 2.97 ± 0.74
0.571ArgTrp: 0.571 ± 0.287
1.028ArgTyr: 1.028 ± 0.475
0.0ArgXaa: 0.0 ± 0.0
Ser
4.455SerAla: 4.455 ± 0.563
1.599SerCys: 1.599 ± 0.633
5.826SerAsp: 5.826 ± 1.252
2.399SerGlu: 2.399 ± 1.026
4.112SerPhe: 4.112 ± 0.981
4.341SerGly: 4.341 ± 1.125
1.599SerHis: 1.599 ± 0.521
4.341SerIle: 4.341 ± 1.162
4.227SerLys: 4.227 ± 0.566
5.483SerLeu: 5.483 ± 0.801
1.485SerMet: 1.485 ± 0.317
4.341SerAsn: 4.341 ± 0.944
1.599SerPro: 1.599 ± 1.062
3.313SerGln: 3.313 ± 1.702
2.17SerArg: 2.17 ± 2.497
5.255SerSer: 5.255 ± 1.436
5.255SerThr: 5.255 ± 0.797
5.369SerVal: 5.369 ± 1.296
0.571SerTrp: 0.571 ± 0.403
3.541SerTyr: 3.541 ± 0.557
0.0SerXaa: 0.0 ± 0.0
Thr
3.084ThrAla: 3.084 ± 0.905
1.142ThrCys: 1.142 ± 0.365
2.97ThrAsp: 2.97 ± 0.521
2.285ThrGlu: 2.285 ± 0.65
3.199ThrPhe: 3.199 ± 0.797
3.541ThrGly: 3.541 ± 1.158
1.485ThrHis: 1.485 ± 0.574
4.455ThrIle: 4.455 ± 0.898
2.742ThrLys: 2.742 ± 0.813
5.483ThrLeu: 5.483 ± 1.456
1.828ThrMet: 1.828 ± 0.616
3.199ThrAsn: 3.199 ± 1.116
2.285ThrPro: 2.285 ± 1.904
1.371ThrGln: 1.371 ± 0.731
2.17ThrArg: 2.17 ± 0.917
3.541ThrSer: 3.541 ± 1.462
5.141ThrThr: 5.141 ± 0.321
6.854ThrVal: 6.854 ± 0.982
0.571ThrTrp: 0.571 ± 0.554
2.285ThrTyr: 2.285 ± 0.534
0.0ThrXaa: 0.0 ± 0.0
Val
5.94ValAla: 5.94 ± 0.933
3.541ValCys: 3.541 ± 0.887
6.283ValAsp: 6.283 ± 1.312
4.455ValGlu: 4.455 ± 1.707
4.227ValPhe: 4.227 ± 1.162
4.569ValGly: 4.569 ± 1.378
1.142ValHis: 1.142 ± 0.263
5.369ValIle: 5.369 ± 1.052
6.626ValLys: 6.626 ± 2.262
7.654ValLeu: 7.654 ± 1.337
1.714ValMet: 1.714 ± 0.752
6.169ValAsn: 6.169 ± 0.555
3.427ValPro: 3.427 ± 0.705
2.856ValGln: 2.856 ± 0.251
2.742ValArg: 2.742 ± 0.399
5.94ValSer: 5.94 ± 1.443
5.483ValThr: 5.483 ± 0.665
9.024ValVal: 9.024 ± 2.927
1.028ValTrp: 1.028 ± 0.269
3.427ValTyr: 3.427 ± 1.007
0.0ValXaa: 0.0 ± 0.0
Trp
0.685TrpAla: 0.685 ± 0.957
0.457TrpCys: 0.457 ± 0.161
0.8TrpAsp: 0.8 ± 0.401
0.571TrpGlu: 0.571 ± 0.329
0.571TrpPhe: 0.571 ± 0.542
0.457TrpGly: 0.457 ± 0.332
0.228TrpHis: 0.228 ± 0.325
0.457TrpIle: 0.457 ± 0.161
0.571TrpLys: 0.571 ± 0.287
1.257TrpLeu: 1.257 ± 0.973
0.228TrpMet: 0.228 ± 0.115
1.257TrpAsn: 1.257 ± 0.893
0.457TrpPro: 0.457 ± 0.403
0.457TrpGln: 0.457 ± 0.35
0.8TrpArg: 0.8 ± 0.316
1.257TrpSer: 1.257 ± 0.464
0.343TrpThr: 0.343 ± 0.172
0.8TrpVal: 0.8 ± 0.705
0.571TrpTrp: 0.571 ± 0.542
1.028TrpTyr: 1.028 ± 0.269
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.084TyrAla: 3.084 ± 0.458
1.942TyrCys: 1.942 ± 0.334
3.541TyrAsp: 3.541 ± 1.425
1.714TyrGlu: 1.714 ± 0.633
1.485TyrPhe: 1.485 ± 0.317
2.742TyrGly: 2.742 ± 0.903
0.685TyrHis: 0.685 ± 0.265
2.285TyrIle: 2.285 ± 1.045
1.942TyrLys: 1.942 ± 0.432
2.285TyrLeu: 2.285 ± 0.895
1.371TyrMet: 1.371 ± 0.51
3.313TyrAsn: 3.313 ± 0.738
1.371TyrPro: 1.371 ± 0.435
0.685TyrGln: 0.685 ± 0.344
1.942TyrArg: 1.942 ± 0.491
3.084TyrSer: 3.084 ± 0.962
2.856TyrThr: 2.856 ± 0.822
5.255TyrVal: 5.255 ± 1.609
0.571TyrTrp: 0.571 ± 0.295
2.742TyrTyr: 2.742 ± 0.428
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6 proteins (8755 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski