Amino acid dipepetide frequency for Helicobacter phage Pt4472G

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.919AlaAla: 0.919 ± 0.46
0.345AlaCys: 0.345 ± 0.2
2.182AlaAsp: 2.182 ± 0.323
2.986AlaGlu: 2.986 ± 0.737
3.79AlaPhe: 3.79 ± 0.869
2.986AlaGly: 2.986 ± 0.645
0.919AlaHis: 0.919 ± 0.307
6.432AlaIle: 6.432 ± 0.913
8.5AlaLys: 8.5 ± 1.131
10.797AlaLeu: 10.797 ± 1.158
1.149AlaMet: 1.149 ± 0.422
5.973AlaAsn: 5.973 ± 0.701
1.378AlaPro: 1.378 ± 0.472
2.182AlaGln: 2.182 ± 0.505
3.676AlaArg: 3.676 ± 0.703
3.446AlaSer: 3.446 ± 0.703
2.297AlaThr: 2.297 ± 0.456
2.068AlaVal: 2.068 ± 0.627
0.459AlaTrp: 0.459 ± 0.248
1.723AlaTyr: 1.723 ± 0.399
0.0AlaXaa: 0.0 ± 0.0
Cys
0.345CysAla: 0.345 ± 0.193
0.23CysCys: 0.23 ± 0.236
0.459CysAsp: 0.459 ± 0.265
0.919CysGlu: 0.919 ± 0.375
0.689CysPhe: 0.689 ± 0.314
0.345CysGly: 0.345 ± 0.246
0.115CysHis: 0.115 ± 0.126
0.689CysIle: 0.689 ± 0.278
0.459CysLys: 0.459 ± 0.227
0.919CysLeu: 0.919 ± 0.426
0.0CysMet: 0.0 ± 0.0
0.23CysAsn: 0.23 ± 0.18
0.345CysPro: 0.345 ± 0.159
0.23CysGln: 0.23 ± 0.162
0.115CysArg: 0.115 ± 0.103
0.23CysSer: 0.23 ± 0.15
0.804CysThr: 0.804 ± 0.344
0.459CysVal: 0.459 ± 0.297
0.0CysTrp: 0.0 ± 0.0
0.345CysTyr: 0.345 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
3.446AspAla: 3.446 ± 0.52
0.345AspCys: 0.345 ± 0.223
1.493AspAsp: 1.493 ± 0.336
3.446AspGlu: 3.446 ± 0.533
4.25AspPhe: 4.25 ± 0.711
2.182AspGly: 2.182 ± 0.42
0.459AspHis: 0.459 ± 0.21
2.872AspIle: 2.872 ± 0.807
5.628AspLys: 5.628 ± 0.839
6.662AspLeu: 6.662 ± 0.881
1.263AspMet: 1.263 ± 0.355
3.905AspAsn: 3.905 ± 0.584
1.378AspPro: 1.378 ± 0.432
0.919AspGln: 0.919 ± 0.337
1.953AspArg: 1.953 ± 0.515
3.101AspSer: 3.101 ± 0.633
1.723AspThr: 1.723 ± 0.484
1.034AspVal: 1.034 ± 0.439
0.115AspTrp: 0.115 ± 0.135
3.446AspTyr: 3.446 ± 0.687
0.0AspXaa: 0.0 ± 0.0
Glu
6.317GluAla: 6.317 ± 0.814
0.345GluCys: 0.345 ± 0.199
2.642GluAsp: 2.642 ± 0.579
4.939GluGlu: 4.939 ± 0.76
4.365GluPhe: 4.365 ± 0.604
1.953GluGly: 1.953 ± 0.518
1.723GluHis: 1.723 ± 0.484
6.662GluIle: 6.662 ± 0.974
9.304GluLys: 9.304 ± 1.317
10.567GluLeu: 10.567 ± 1.146
1.723GluMet: 1.723 ± 0.419
6.777GluAsn: 6.777 ± 0.973
1.493GluPro: 1.493 ± 0.363
5.743GluGln: 5.743 ± 0.964
4.48GluArg: 4.48 ± 0.493
6.777GluSer: 6.777 ± 0.976
4.939GluThr: 4.939 ± 0.673
3.676GluVal: 3.676 ± 0.85
0.345GluTrp: 0.345 ± 0.198
2.412GluTyr: 2.412 ± 0.463
0.0GluXaa: 0.0 ± 0.0
Phe
1.723PheAla: 1.723 ± 0.473
0.919PheCys: 0.919 ± 0.446
2.986PheAsp: 2.986 ± 0.569
3.79PheGlu: 3.79 ± 0.467
2.986PhePhe: 2.986 ± 0.664
1.493PheGly: 1.493 ± 0.356
0.689PheHis: 0.689 ± 0.198
2.757PheIle: 2.757 ± 0.581
6.662PheLys: 6.662 ± 1.125
5.858PheLeu: 5.858 ± 1.161
0.689PheMet: 0.689 ± 0.299
3.446PheAsn: 3.446 ± 0.622
0.459PhePro: 0.459 ± 0.226
0.919PheGln: 0.919 ± 0.458
1.838PheArg: 1.838 ± 0.419
4.48PheSer: 4.48 ± 0.64
2.642PheThr: 2.642 ± 0.544
2.297PheVal: 2.297 ± 0.458
0.115PheTrp: 0.115 ± 0.1
2.068PheTyr: 2.068 ± 0.543
0.0PheXaa: 0.0 ± 0.0
Gly
4.135GlyAla: 4.135 ± 1.138
0.345GlyCys: 0.345 ± 0.184
1.723GlyAsp: 1.723 ± 0.435
2.757GlyGlu: 2.757 ± 0.679
2.872GlyPhe: 2.872 ± 0.564
3.101GlyGly: 3.101 ± 0.829
0.23GlyHis: 0.23 ± 0.149
3.216GlyIle: 3.216 ± 0.485
2.757GlyLys: 2.757 ± 0.689
5.743GlyLeu: 5.743 ± 0.989
1.493GlyMet: 1.493 ± 0.434
2.986GlyAsn: 2.986 ± 0.505
0.115GlyPro: 0.115 ± 0.135
1.149GlyGln: 1.149 ± 0.47
1.378GlyArg: 1.378 ± 0.352
2.757GlySer: 2.757 ± 0.516
0.919GlyThr: 0.919 ± 0.304
3.676GlyVal: 3.676 ± 1.064
0.0GlyTrp: 0.0 ± 0.0
2.068GlyTyr: 2.068 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
0.689HisAla: 0.689 ± 0.267
0.115HisCys: 0.115 ± 0.118
1.493HisAsp: 1.493 ± 0.566
1.149HisGlu: 1.149 ± 0.407
0.574HisPhe: 0.574 ± 0.235
0.23HisGly: 0.23 ± 0.172
0.115HisHis: 0.115 ± 0.1
1.378HisIle: 1.378 ± 0.478
1.953HisLys: 1.953 ± 0.463
0.574HisLeu: 0.574 ± 0.282
0.115HisMet: 0.115 ± 0.116
1.034HisAsn: 1.034 ± 0.37
0.23HisPro: 0.23 ± 0.129
0.115HisGln: 0.115 ± 0.093
0.459HisArg: 0.459 ± 0.211
1.034HisSer: 1.034 ± 0.315
1.034HisThr: 1.034 ± 0.383
0.574HisVal: 0.574 ± 0.233
0.115HisTrp: 0.115 ± 0.093
0.919HisTyr: 0.919 ± 0.292
0.0HisXaa: 0.0 ± 0.0
Ile
4.595IleAla: 4.595 ± 0.792
0.689IleCys: 0.689 ± 0.264
3.676IleAsp: 3.676 ± 0.669
4.939IleGlu: 4.939 ± 0.673
2.527IlePhe: 2.527 ± 0.536
1.608IleGly: 1.608 ± 0.61
0.804IleHis: 0.804 ± 0.335
3.331IleIle: 3.331 ± 0.667
9.074IleLys: 9.074 ± 1.134
9.189IleLeu: 9.189 ± 0.917
1.034IleMet: 1.034 ± 0.291
5.054IleAsn: 5.054 ± 0.691
1.378IlePro: 1.378 ± 0.487
4.02IleGln: 4.02 ± 0.682
2.986IleArg: 2.986 ± 0.576
3.216IleSer: 3.216 ± 0.544
4.824IleThr: 4.824 ± 0.57
3.676IleVal: 3.676 ± 0.644
0.23IleTrp: 0.23 ± 0.141
2.412IleTyr: 2.412 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
7.581LysAla: 7.581 ± 1.1
0.689LysCys: 0.689 ± 0.357
7.696LysAsp: 7.696 ± 1.39
13.669LysGlu: 13.669 ± 1.312
2.986LysPhe: 2.986 ± 0.633
4.02LysGly: 4.02 ± 0.718
2.872LysHis: 2.872 ± 0.742
9.189LysIle: 9.189 ± 1.28
9.993LysLys: 9.993 ± 1.22
7.926LysLeu: 7.926 ± 1.033
1.953LysMet: 1.953 ± 0.416
9.074LysAsn: 9.074 ± 1.235
3.331LysPro: 3.331 ± 0.612
5.628LysGln: 5.628 ± 0.9
4.135LysArg: 4.135 ± 0.646
5.169LysSer: 5.169 ± 0.728
5.973LysThr: 5.973 ± 0.687
5.284LysVal: 5.284 ± 0.773
0.574LysTrp: 0.574 ± 0.241
2.527LysTyr: 2.527 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
5.743LeuAla: 5.743 ± 0.757
1.608LeuCys: 1.608 ± 0.541
5.054LeuAsp: 5.054 ± 0.598
13.324LeuGlu: 13.324 ± 1.589
4.48LeuPhe: 4.48 ± 0.739
5.858LeuGly: 5.858 ± 0.926
0.345LeuHis: 0.345 ± 0.181
5.858LeuIle: 5.858 ± 0.897
16.196LeuLys: 16.196 ± 1.539
7.236LeuLeu: 7.236 ± 0.745
1.723LeuMet: 1.723 ± 0.406
10.567LeuAsn: 10.567 ± 1.269
1.263LeuPro: 1.263 ± 0.371
5.513LeuGln: 5.513 ± 1.081
4.135LeuArg: 4.135 ± 0.979
7.007LeuSer: 7.007 ± 0.969
3.216LeuThr: 3.216 ± 0.709
4.25LeuVal: 4.25 ± 1.017
0.574LeuTrp: 0.574 ± 0.278
1.493LeuTyr: 1.493 ± 0.445
0.0LeuXaa: 0.0 ± 0.0
Met
0.689MetAla: 0.689 ± 0.253
0.0MetCys: 0.0 ± 0.0
1.493MetAsp: 1.493 ± 0.398
0.804MetGlu: 0.804 ± 0.276
1.263MetPhe: 1.263 ± 0.427
1.378MetGly: 1.378 ± 0.42
0.345MetHis: 0.345 ± 0.18
1.608MetIle: 1.608 ± 0.403
1.493MetLys: 1.493 ± 0.47
1.493MetLeu: 1.493 ± 0.389
0.115MetMet: 0.115 ± 0.106
2.068MetAsn: 2.068 ± 0.494
1.378MetPro: 1.378 ± 0.419
1.608MetGln: 1.608 ± 0.435
0.919MetArg: 0.919 ± 0.302
0.689MetSer: 0.689 ± 0.218
0.345MetThr: 0.345 ± 0.233
0.689MetVal: 0.689 ± 0.251
0.115MetTrp: 0.115 ± 0.1
0.459MetTyr: 0.459 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
8.5AsnAla: 8.5 ± 1.111
0.115AsnCys: 0.115 ± 0.104
3.331AsnAsp: 3.331 ± 0.616
7.811AsnGlu: 7.811 ± 1.014
3.331AsnPhe: 3.331 ± 0.655
2.182AsnGly: 2.182 ± 0.468
1.378AsnHis: 1.378 ± 0.53
4.595AsnIle: 4.595 ± 0.829
8.27AsnLys: 8.27 ± 1.063
7.466AsnLeu: 7.466 ± 1.144
1.149AsnMet: 1.149 ± 0.328
6.203AsnAsn: 6.203 ± 0.8
3.331AsnPro: 3.331 ± 0.748
4.02AsnGln: 4.02 ± 0.716
2.182AsnArg: 2.182 ± 0.409
5.169AsnSer: 5.169 ± 0.723
3.216AsnThr: 3.216 ± 0.724
2.182AsnVal: 2.182 ± 0.51
0.23AsnTrp: 0.23 ± 0.144
4.709AsnTyr: 4.709 ± 0.586
0.0AsnXaa: 0.0 ± 0.0
Pro
0.459ProAla: 0.459 ± 0.29
0.23ProCys: 0.23 ± 0.168
0.804ProAsp: 0.804 ± 0.304
1.263ProGlu: 1.263 ± 0.401
1.838ProPhe: 1.838 ± 0.493
0.345ProGly: 0.345 ± 0.276
0.23ProHis: 0.23 ± 0.156
1.723ProIle: 1.723 ± 0.387
3.216ProLys: 3.216 ± 0.682
2.527ProLeu: 2.527 ± 0.714
0.459ProMet: 0.459 ± 0.276
2.297ProAsn: 2.297 ± 0.468
0.23ProPro: 0.23 ± 0.174
0.345ProGln: 0.345 ± 0.169
0.919ProArg: 0.919 ± 0.284
2.182ProSer: 2.182 ± 0.469
2.412ProThr: 2.412 ± 0.751
1.149ProVal: 1.149 ± 0.42
0.0ProTrp: 0.0 ± 0.0
1.263ProTyr: 1.263 ± 0.49
0.0ProXaa: 0.0 ± 0.0
Gln
5.973GlnAla: 5.973 ± 0.838
0.23GlnCys: 0.23 ± 0.254
1.953GlnAsp: 1.953 ± 0.459
5.054GlnGlu: 5.054 ± 0.804
1.263GlnPhe: 1.263 ± 0.39
2.642GlnGly: 2.642 ± 0.511
0.345GlnHis: 0.345 ± 0.226
2.068GlnIle: 2.068 ± 0.478
4.595GlnLys: 4.595 ± 0.659
3.561GlnLeu: 3.561 ± 0.66
0.919GlnMet: 0.919 ± 0.322
3.561GlnAsn: 3.561 ± 0.513
0.574GlnPro: 0.574 ± 0.219
1.953GlnGln: 1.953 ± 0.473
1.953GlnArg: 1.953 ± 0.482
2.757GlnSer: 2.757 ± 0.504
2.068GlnThr: 2.068 ± 0.393
1.723GlnVal: 1.723 ± 0.313
0.345GlnTrp: 0.345 ± 0.176
0.689GlnTyr: 0.689 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
2.412ArgAla: 2.412 ± 0.496
0.115ArgCys: 0.115 ± 0.103
2.412ArgAsp: 2.412 ± 0.333
4.824ArgGlu: 4.824 ± 0.927
2.412ArgPhe: 2.412 ± 0.479
1.838ArgGly: 1.838 ± 0.513
0.689ArgHis: 0.689 ± 0.297
2.527ArgIle: 2.527 ± 0.509
3.676ArgLys: 3.676 ± 0.558
4.135ArgLeu: 4.135 ± 0.761
0.689ArgMet: 0.689 ± 0.276
1.838ArgAsn: 1.838 ± 0.483
1.034ArgPro: 1.034 ± 0.389
1.953ArgGln: 1.953 ± 0.554
1.378ArgArg: 1.378 ± 0.44
2.068ArgSer: 2.068 ± 0.445
1.953ArgThr: 1.953 ± 0.519
1.953ArgVal: 1.953 ± 0.485
0.115ArgTrp: 0.115 ± 0.093
1.608ArgTyr: 1.608 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
4.48SerAla: 4.48 ± 0.672
0.574SerCys: 0.574 ± 0.364
4.595SerAsp: 4.595 ± 0.536
5.743SerGlu: 5.743 ± 0.973
3.905SerPhe: 3.905 ± 0.722
3.331SerGly: 3.331 ± 0.547
0.574SerHis: 0.574 ± 0.215
3.101SerIle: 3.101 ± 0.567
5.628SerLys: 5.628 ± 0.831
7.351SerLeu: 7.351 ± 1.037
1.608SerMet: 1.608 ± 0.487
4.135SerAsn: 4.135 ± 0.815
1.149SerPro: 1.149 ± 0.283
2.642SerGln: 2.642 ± 0.56
1.953SerArg: 1.953 ± 0.514
2.297SerSer: 2.297 ± 0.433
1.953SerThr: 1.953 ± 0.473
4.824SerVal: 4.824 ± 0.88
0.345SerTrp: 0.345 ± 0.163
2.527SerTyr: 2.527 ± 0.498
0.0SerXaa: 0.0 ± 0.0
Thr
2.182ThrAla: 2.182 ± 0.607
0.345ThrCys: 0.345 ± 0.203
2.527ThrAsp: 2.527 ± 0.597
3.216ThrGlu: 3.216 ± 0.528
0.919ThrPhe: 0.919 ± 0.265
2.297ThrGly: 2.297 ± 0.475
1.149ThrHis: 1.149 ± 0.464
3.561ThrIle: 3.561 ± 0.651
4.939ThrLys: 4.939 ± 0.831
4.25ThrLeu: 4.25 ± 0.781
1.034ThrMet: 1.034 ± 0.244
3.446ThrAsn: 3.446 ± 0.478
2.642ThrPro: 2.642 ± 0.516
2.297ThrGln: 2.297 ± 0.512
1.378ThrArg: 1.378 ± 0.41
3.905ThrSer: 3.905 ± 0.808
3.561ThrThr: 3.561 ± 0.641
0.804ThrVal: 0.804 ± 0.301
0.574ThrTrp: 0.574 ± 0.22
1.378ThrTyr: 1.378 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
2.182ValAla: 2.182 ± 0.644
0.459ValCys: 0.459 ± 0.24
1.953ValAsp: 1.953 ± 0.458
3.101ValGlu: 3.101 ± 0.491
2.182ValPhe: 2.182 ± 0.464
3.561ValGly: 3.561 ± 0.807
0.115ValHis: 0.115 ± 0.128
4.25ValIle: 4.25 ± 0.708
3.676ValLys: 3.676 ± 0.589
5.169ValLeu: 5.169 ± 0.792
0.919ValMet: 0.919 ± 0.397
2.872ValAsn: 2.872 ± 0.905
0.919ValPro: 0.919 ± 0.357
0.919ValGln: 0.919 ± 0.364
1.838ValArg: 1.838 ± 0.442
3.79ValSer: 3.79 ± 0.699
1.378ValThr: 1.378 ± 0.482
2.986ValVal: 2.986 ± 0.683
0.345ValTrp: 0.345 ± 0.21
1.723ValTyr: 1.723 ± 0.876
0.0ValXaa: 0.0 ± 0.0
Trp
0.115TrpAla: 0.115 ± 0.135
0.0TrpCys: 0.0 ± 0.0
0.0TrpAsp: 0.0 ± 0.0
0.574TrpGlu: 0.574 ± 0.37
0.0TrpPhe: 0.0 ± 0.0
0.574TrpGly: 0.574 ± 0.318
0.115TrpHis: 0.115 ± 0.103
0.23TrpIle: 0.23 ± 0.161
0.115TrpLys: 0.115 ± 0.103
0.115TrpLeu: 0.115 ± 0.106
0.23TrpMet: 0.23 ± 0.156
0.804TrpAsn: 0.804 ± 0.342
0.0TrpPro: 0.0 ± 0.0
0.115TrpGln: 0.115 ± 0.093
0.23TrpArg: 0.23 ± 0.144
0.459TrpSer: 0.459 ± 0.259
0.0TrpThr: 0.0 ± 0.0
0.689TrpVal: 0.689 ± 0.248
0.0TrpTrp: 0.0 ± 0.0
0.345TrpTyr: 0.345 ± 0.248
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.723TyrAla: 1.723 ± 0.379
0.23TyrCys: 0.23 ± 0.164
1.149TyrAsp: 1.149 ± 0.285
2.872TyrGlu: 2.872 ± 0.667
2.182TyrPhe: 2.182 ± 0.607
1.493TyrGly: 1.493 ± 0.38
0.689TyrHis: 0.689 ± 0.252
3.216TyrIle: 3.216 ± 0.643
4.135TyrLys: 4.135 ± 0.53
3.905TyrLeu: 3.905 ± 0.884
0.574TyrMet: 0.574 ± 0.231
3.101TyrAsn: 3.101 ± 0.643
1.378TyrPro: 1.378 ± 0.284
1.953TyrGln: 1.953 ± 0.411
1.723TyrArg: 1.723 ± 0.396
2.182TyrSer: 2.182 ± 0.568
1.149TyrThr: 1.149 ± 0.331
0.345TyrVal: 0.345 ± 0.196
0.115TyrTrp: 0.115 ± 0.12
1.608TyrTyr: 1.608 ± 0.462
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 32 proteins (8707 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski