Amino acid dipepetide frequency for Mycobacterium phage Philly

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.156AlaAla: 16.156 ± 1.266
0.87AlaCys: 0.87 ± 0.207
7.689AlaAsp: 7.689 ± 0.529
7.643AlaGlu: 7.643 ± 0.628
2.563AlaPhe: 2.563 ± 0.349
9.428AlaGly: 9.428 ± 1.182
1.785AlaHis: 1.785 ± 0.323
5.263AlaIle: 5.263 ± 0.508
3.295AlaLys: 3.295 ± 0.376
10.298AlaLeu: 10.298 ± 0.913
2.655AlaMet: 2.655 ± 0.335
3.341AlaAsn: 3.341 ± 0.423
6.133AlaPro: 6.133 ± 0.61
2.883AlaGln: 2.883 ± 0.366
7.781AlaArg: 7.781 ± 0.706
5.263AlaSer: 5.263 ± 0.495
6.041AlaThr: 6.041 ± 0.605
9.84AlaVal: 9.84 ± 0.556
2.7AlaTrp: 2.7 ± 0.347
2.472AlaTyr: 2.472 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
1.007CysAla: 1.007 ± 0.275
0.183CysCys: 0.183 ± 0.105
0.87CysAsp: 0.87 ± 0.196
0.824CysGlu: 0.824 ± 0.242
0.183CysPhe: 0.183 ± 0.103
1.648CysGly: 1.648 ± 0.414
0.412CysHis: 0.412 ± 0.145
0.412CysIle: 0.412 ± 0.154
0.183CysLys: 0.183 ± 0.09
0.549CysLeu: 0.549 ± 0.199
0.137CysMet: 0.137 ± 0.097
0.412CysAsn: 0.412 ± 0.146
1.373CysPro: 1.373 ± 0.335
0.32CysGln: 0.32 ± 0.128
0.87CysArg: 0.87 ± 0.234
0.687CysSer: 0.687 ± 0.202
0.458CysThr: 0.458 ± 0.156
0.595CysVal: 0.595 ± 0.205
0.32CysTrp: 0.32 ± 0.132
0.183CysTyr: 0.183 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
7.643AspAla: 7.643 ± 0.516
0.641AspCys: 0.641 ± 0.15
6.133AspAsp: 6.133 ± 0.734
5.218AspGlu: 5.218 ± 0.519
1.739AspPhe: 1.739 ± 0.25
6.453AspGly: 6.453 ± 0.522
1.648AspHis: 1.648 ± 0.365
2.746AspIle: 2.746 ± 0.34
2.151AspLys: 2.151 ± 0.244
5.721AspLeu: 5.721 ± 0.485
1.465AspMet: 1.465 ± 0.22
1.877AspAsn: 1.877 ± 0.288
4.989AspPro: 4.989 ± 0.527
2.426AspGln: 2.426 ± 0.339
4.806AspArg: 4.806 ± 0.566
3.021AspSer: 3.021 ± 0.407
3.753AspThr: 3.753 ± 0.409
4.44AspVal: 4.44 ± 0.489
1.19AspTrp: 1.19 ± 0.202
1.098AspTyr: 1.098 ± 0.205
0.0AspXaa: 0.0 ± 0.0
Glu
7.277GluAla: 7.277 ± 0.682
1.007GluCys: 1.007 ± 0.292
3.799GluAsp: 3.799 ± 0.518
2.426GluGlu: 2.426 ± 0.434
1.785GluPhe: 1.785 ± 0.23
4.44GluGly: 4.44 ± 0.437
1.327GluHis: 1.327 ± 0.27
3.845GluIle: 3.845 ± 0.438
2.151GluLys: 2.151 ± 0.317
4.211GluLeu: 4.211 ± 0.49
1.785GluMet: 1.785 ± 0.266
1.465GluAsn: 1.465 ± 0.237
3.89GluPro: 3.89 ± 0.483
2.334GluGln: 2.334 ± 0.374
4.394GluArg: 4.394 ± 0.454
3.021GluSer: 3.021 ± 0.407
3.295GluThr: 3.295 ± 0.504
4.485GluVal: 4.485 ± 0.47
1.419GluTrp: 1.419 ± 0.271
1.51GluTyr: 1.51 ± 0.246
0.0GluXaa: 0.0 ± 0.0
Phe
2.975PheAla: 2.975 ± 0.267
0.183PheCys: 0.183 ± 0.089
2.7PheAsp: 2.7 ± 0.353
1.465PheGlu: 1.465 ± 0.264
0.503PhePhe: 0.503 ± 0.134
2.7PheGly: 2.7 ± 0.418
0.503PheHis: 0.503 ± 0.183
0.824PheIle: 0.824 ± 0.207
0.778PheLys: 0.778 ± 0.195
1.922PheLeu: 1.922 ± 0.248
0.32PheMet: 0.32 ± 0.095
0.732PheAsn: 0.732 ± 0.148
1.51PhePro: 1.51 ± 0.231
0.687PheGln: 0.687 ± 0.179
1.785PheArg: 1.785 ± 0.296
1.282PheSer: 1.282 ± 0.239
1.831PheThr: 1.831 ± 0.374
2.197PheVal: 2.197 ± 0.329
0.641PheTrp: 0.641 ± 0.187
0.503PheTyr: 0.503 ± 0.163
0.0PheXaa: 0.0 ± 0.0
Gly
8.055GlyAla: 8.055 ± 1.262
0.87GlyCys: 0.87 ± 0.288
4.989GlyAsp: 4.989 ± 0.438
5.218GlyGlu: 5.218 ± 0.526
2.609GlyPhe: 2.609 ± 0.332
13.136GlyGly: 13.136 ± 2.578
1.419GlyHis: 1.419 ± 0.25
4.897GlyIle: 4.897 ± 0.535
4.211GlyLys: 4.211 ± 0.482
7.689GlyLeu: 7.689 ± 0.809
2.426GlyMet: 2.426 ± 0.451
3.204GlyAsn: 3.204 ± 0.416
3.89GlyPro: 3.89 ± 0.467
2.883GlyGln: 2.883 ± 0.468
5.355GlyArg: 5.355 ± 0.531
5.675GlySer: 5.675 ± 0.655
6.545GlyThr: 6.545 ± 0.689
7.643GlyVal: 7.643 ± 0.624
2.014GlyTrp: 2.014 ± 0.337
1.922GlyTyr: 1.922 ± 0.309
0.0GlyXaa: 0.0 ± 0.0
His
1.785HisAla: 1.785 ± 0.314
0.32HisCys: 0.32 ± 0.122
1.373HisAsp: 1.373 ± 0.254
0.732HisGlu: 0.732 ± 0.267
0.641HisPhe: 0.641 ± 0.163
1.648HisGly: 1.648 ± 0.282
0.458HisHis: 0.458 ± 0.155
0.595HisIle: 0.595 ± 0.169
0.458HisLys: 0.458 ± 0.144
1.419HisLeu: 1.419 ± 0.249
0.412HisMet: 0.412 ± 0.159
0.366HisAsn: 0.366 ± 0.15
1.053HisPro: 1.053 ± 0.225
0.503HisGln: 0.503 ± 0.187
1.556HisArg: 1.556 ± 0.263
0.87HisSer: 0.87 ± 0.197
0.961HisThr: 0.961 ± 0.24
1.098HisVal: 1.098 ± 0.246
0.458HisTrp: 0.458 ± 0.147
0.595HisTyr: 0.595 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
5.95IleAla: 5.95 ± 0.461
0.412IleCys: 0.412 ± 0.13
3.936IleAsp: 3.936 ± 0.366
3.753IleGlu: 3.753 ± 0.368
1.098IlePhe: 1.098 ± 0.196
3.433IleGly: 3.433 ± 0.484
0.778IleHis: 0.778 ± 0.156
2.151IleIle: 2.151 ± 0.428
1.602IleLys: 1.602 ± 0.331
3.021IleLeu: 3.021 ± 0.375
0.778IleMet: 0.778 ± 0.16
1.556IleAsn: 1.556 ± 0.268
2.7IlePro: 2.7 ± 0.317
1.739IleGln: 1.739 ± 0.321
3.616IleArg: 3.616 ± 0.349
2.655IleSer: 2.655 ± 0.322
3.295IleThr: 3.295 ± 0.424
4.028IleVal: 4.028 ± 0.436
0.503IleTrp: 0.503 ± 0.128
0.824IleTyr: 0.824 ± 0.182
0.0IleXaa: 0.0 ± 0.0
Lys
4.165LysAla: 4.165 ± 0.448
0.32LysCys: 0.32 ± 0.113
1.831LysAsp: 1.831 ± 0.317
1.282LysGlu: 1.282 ± 0.228
1.19LysPhe: 1.19 ± 0.21
2.563LysGly: 2.563 ± 0.298
0.458LysHis: 0.458 ± 0.146
1.877LysIle: 1.877 ± 0.367
0.87LysLys: 0.87 ± 0.202
3.112LysLeu: 3.112 ± 0.329
0.549LysMet: 0.549 ± 0.203
1.19LysAsn: 1.19 ± 0.258
1.693LysPro: 1.693 ± 0.242
0.87LysGln: 0.87 ± 0.26
2.06LysArg: 2.06 ± 0.349
2.06LysSer: 2.06 ± 0.312
1.327LysThr: 1.327 ± 0.326
3.112LysVal: 3.112 ± 0.394
0.595LysTrp: 0.595 ± 0.132
1.053LysTyr: 1.053 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
9.062LeuAla: 9.062 ± 0.823
0.915LeuCys: 0.915 ± 0.213
5.721LeuAsp: 5.721 ± 0.486
3.204LeuGlu: 3.204 ± 0.405
1.968LeuPhe: 1.968 ± 0.315
8.193LeuGly: 8.193 ± 0.733
1.556LeuHis: 1.556 ± 0.304
4.119LeuIle: 4.119 ± 0.396
2.563LeuLys: 2.563 ± 0.453
5.63LeuLeu: 5.63 ± 0.54
1.693LeuMet: 1.693 ± 0.282
1.968LeuAsn: 1.968 ± 0.304
4.943LeuPro: 4.943 ± 0.838
1.877LeuGln: 1.877 ± 0.303
5.401LeuArg: 5.401 ± 0.556
4.44LeuSer: 4.44 ± 0.644
6.591LeuThr: 6.591 ± 0.736
5.309LeuVal: 5.309 ± 0.558
1.373LeuTrp: 1.373 ± 0.259
2.288LeuTyr: 2.288 ± 0.339
0.0LeuXaa: 0.0 ± 0.0
Met
3.158MetAla: 3.158 ± 0.484
0.183MetCys: 0.183 ± 0.085
1.007MetAsp: 1.007 ± 0.198
0.87MetGlu: 0.87 ± 0.156
0.549MetPhe: 0.549 ± 0.158
1.602MetGly: 1.602 ± 0.29
0.229MetHis: 0.229 ± 0.096
1.236MetIle: 1.236 ± 0.258
0.732MetLys: 0.732 ± 0.182
1.831MetLeu: 1.831 ± 0.321
0.366MetMet: 0.366 ± 0.123
0.595MetAsn: 0.595 ± 0.174
1.831MetPro: 1.831 ± 0.279
0.687MetGln: 0.687 ± 0.169
0.915MetArg: 0.915 ± 0.199
1.602MetSer: 1.602 ± 0.278
2.655MetThr: 2.655 ± 0.42
1.419MetVal: 1.419 ± 0.259
0.275MetTrp: 0.275 ± 0.122
0.366MetTyr: 0.366 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
4.211AsnAla: 4.211 ± 0.516
0.32AsnCys: 0.32 ± 0.116
1.693AsnAsp: 1.693 ± 0.293
1.053AsnGlu: 1.053 ± 0.191
0.549AsnPhe: 0.549 ± 0.166
3.067AsnGly: 3.067 ± 0.362
0.595AsnHis: 0.595 ± 0.194
0.961AsnIle: 0.961 ± 0.226
0.824AsnLys: 0.824 ± 0.161
2.288AsnLeu: 2.288 ± 0.321
0.458AsnMet: 0.458 ± 0.173
0.641AsnAsn: 0.641 ± 0.155
3.112AsnPro: 3.112 ± 0.423
0.412AsnGln: 0.412 ± 0.144
1.693AsnArg: 1.693 ± 0.244
1.098AsnSer: 1.098 ± 0.234
2.06AsnThr: 2.06 ± 0.333
2.243AsnVal: 2.243 ± 0.312
0.824AsnTrp: 0.824 ± 0.211
0.595AsnTyr: 0.595 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
6.27ProAla: 6.27 ± 0.597
0.778ProCys: 0.778 ± 0.294
5.218ProAsp: 5.218 ± 0.678
6.225ProGlu: 6.225 ± 0.616
1.556ProPhe: 1.556 ± 0.281
5.813ProGly: 5.813 ± 0.525
0.641ProHis: 0.641 ± 0.152
2.975ProIle: 2.975 ± 0.352
1.419ProLys: 1.419 ± 0.229
3.341ProLeu: 3.341 ± 0.296
1.465ProMet: 1.465 ± 0.284
1.465ProAsn: 1.465 ± 0.347
4.44ProPro: 4.44 ± 0.593
1.19ProGln: 1.19 ± 0.208
3.204ProArg: 3.204 ± 0.393
3.524ProSer: 3.524 ± 0.473
3.707ProThr: 3.707 ± 0.359
5.355ProVal: 5.355 ± 0.48
1.327ProTrp: 1.327 ± 0.249
0.961ProTyr: 0.961 ± 0.223
0.0ProXaa: 0.0 ± 0.0
Gln
4.119GlnAla: 4.119 ± 0.409
0.549GlnCys: 0.549 ± 0.178
1.144GlnAsp: 1.144 ± 0.24
1.465GlnGlu: 1.465 ± 0.316
1.053GlnPhe: 1.053 ± 0.23
2.426GlnGly: 2.426 ± 0.367
0.549GlnHis: 0.549 ± 0.175
1.831GlnIle: 1.831 ± 0.338
0.824GlnLys: 0.824 ± 0.215
2.426GlnLeu: 2.426 ± 0.334
1.144GlnMet: 1.144 ± 0.217
0.549GlnAsn: 0.549 ± 0.145
1.693GlnPro: 1.693 ± 0.244
1.877GlnGln: 1.877 ± 0.338
2.517GlnArg: 2.517 ± 0.396
1.373GlnSer: 1.373 ± 0.23
1.922GlnThr: 1.922 ± 0.319
1.968GlnVal: 1.968 ± 0.275
0.549GlnTrp: 0.549 ± 0.152
1.144GlnTyr: 1.144 ± 0.198
0.0GlnXaa: 0.0 ± 0.0
Arg
6.041ArgAla: 6.041 ± 0.516
1.007ArgCys: 1.007 ± 0.207
4.668ArgAsp: 4.668 ± 0.403
4.44ArgGlu: 4.44 ± 0.525
1.968ArgPhe: 1.968 ± 0.324
4.668ArgGly: 4.668 ± 0.478
1.373ArgHis: 1.373 ± 0.258
2.975ArgIle: 2.975 ± 0.436
1.831ArgLys: 1.831 ± 0.303
5.584ArgLeu: 5.584 ± 0.564
1.739ArgMet: 1.739 ± 0.31
2.197ArgAsn: 2.197 ± 0.285
3.753ArgPro: 3.753 ± 0.487
2.655ArgGln: 2.655 ± 0.349
6.041ArgArg: 6.041 ± 0.974
3.341ArgSer: 3.341 ± 0.413
3.89ArgThr: 3.89 ± 0.365
4.806ArgVal: 4.806 ± 0.507
1.785ArgTrp: 1.785 ± 0.362
2.105ArgTyr: 2.105 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
6.041SerAla: 6.041 ± 0.51
0.366SerCys: 0.366 ± 0.147
3.478SerAsp: 3.478 ± 0.4
2.883SerGlu: 2.883 ± 0.4
1.373SerPhe: 1.373 ± 0.281
6.408SerGly: 6.408 ± 0.637
0.915SerHis: 0.915 ± 0.171
2.563SerIle: 2.563 ± 0.34
2.105SerLys: 2.105 ± 0.333
4.165SerLeu: 4.165 ± 0.534
1.144SerMet: 1.144 ± 0.224
1.693SerAsn: 1.693 ± 0.31
3.387SerPro: 3.387 ± 0.351
1.922SerGln: 1.922 ± 0.324
3.433SerArg: 3.433 ± 0.479
2.609SerSer: 2.609 ± 0.371
3.57SerThr: 3.57 ± 0.414
3.387SerVal: 3.387 ± 0.389
1.007SerTrp: 1.007 ± 0.268
1.007SerTyr: 1.007 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
7.735ThrAla: 7.735 ± 0.64
0.87ThrCys: 0.87 ± 0.277
3.89ThrAsp: 3.89 ± 0.378
3.57ThrGlu: 3.57 ± 0.452
1.922ThrPhe: 1.922 ± 0.199
6.591ThrGly: 6.591 ± 0.679
0.595ThrHis: 0.595 ± 0.162
3.661ThrIle: 3.661 ± 0.442
2.563ThrLys: 2.563 ± 0.304
5.218ThrLeu: 5.218 ± 0.464
1.236ThrMet: 1.236 ± 0.245
1.419ThrAsn: 1.419 ± 0.27
4.394ThrPro: 4.394 ± 0.427
1.785ThrGln: 1.785 ± 0.345
4.165ThrArg: 4.165 ± 0.51
3.295ThrSer: 3.295 ± 0.408
3.982ThrThr: 3.982 ± 0.555
5.675ThrVal: 5.675 ± 0.468
1.236ThrTrp: 1.236 ± 0.277
1.236ThrTyr: 1.236 ± 0.237
0.0ThrXaa: 0.0 ± 0.0
Val
8.559ValAla: 8.559 ± 0.696
0.824ValCys: 0.824 ± 0.229
5.767ValAsp: 5.767 ± 0.654
5.08ValGlu: 5.08 ± 0.509
1.465ValPhe: 1.465 ± 0.263
7.14ValGly: 7.14 ± 0.498
1.465ValHis: 1.465 ± 0.283
3.25ValIle: 3.25 ± 0.347
2.38ValLys: 2.38 ± 0.33
6.179ValLeu: 6.179 ± 0.52
1.465ValMet: 1.465 ± 0.228
3.067ValAsn: 3.067 ± 0.365
4.073ValPro: 4.073 ± 0.43
2.334ValGln: 2.334 ± 0.272
3.845ValArg: 3.845 ± 0.603
5.126ValSer: 5.126 ± 0.548
5.721ValThr: 5.721 ± 0.632
6.636ValVal: 6.636 ± 0.632
1.236ValTrp: 1.236 ± 0.238
1.968ValTyr: 1.968 ± 0.291
0.0ValXaa: 0.0 ± 0.0
Trp
1.693TrpAla: 1.693 ± 0.304
0.549TrpCys: 0.549 ± 0.172
1.465TrpAsp: 1.465 ± 0.277
1.373TrpGlu: 1.373 ± 0.26
0.595TrpPhe: 0.595 ± 0.16
1.053TrpGly: 1.053 ± 0.187
0.366TrpHis: 0.366 ± 0.129
0.778TrpIle: 0.778 ± 0.201
0.824TrpLys: 0.824 ± 0.177
1.602TrpLeu: 1.602 ± 0.301
0.458TrpMet: 0.458 ± 0.142
0.412TrpAsn: 0.412 ± 0.144
1.19TrpPro: 1.19 ± 0.247
0.641TrpGln: 0.641 ± 0.183
1.51TrpArg: 1.51 ± 0.28
1.602TrpSer: 1.602 ± 0.248
1.785TrpThr: 1.785 ± 0.373
1.19TrpVal: 1.19 ± 0.257
0.229TrpTrp: 0.229 ± 0.109
0.595TrpTyr: 0.595 ± 0.172
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.243TyrAla: 2.243 ± 0.289
0.458TyrCys: 0.458 ± 0.162
1.785TyrAsp: 1.785 ± 0.294
1.144TyrGlu: 1.144 ± 0.19
0.732TyrPhe: 0.732 ± 0.167
2.243TyrGly: 2.243 ± 0.354
0.229TyrHis: 0.229 ± 0.104
0.87TyrIle: 0.87 ± 0.243
0.412TyrLys: 0.412 ± 0.126
2.609TyrLeu: 2.609 ± 0.379
0.366TyrMet: 0.366 ± 0.147
0.549TyrAsn: 0.549 ± 0.153
0.824TyrPro: 0.824 ± 0.187
1.098TyrGln: 1.098 ± 0.217
1.922TyrArg: 1.922 ± 0.357
0.915TyrSer: 0.915 ± 0.182
1.648TyrThr: 1.648 ± 0.288
2.151TyrVal: 2.151 ± 0.341
0.229TyrTrp: 0.229 ± 0.094
0.366TyrTyr: 0.366 ± 0.114
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (21850 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski