Amino acid dipepetide frequency for Pseudomonas phage YMC12/01/R24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.322AlaAla: 17.322 ± 1.788
1.369AlaCys: 1.369 ± 0.356
6.573AlaAsp: 6.573 ± 0.773
8.353AlaGlu: 8.353 ± 0.961
3.903AlaPhe: 3.903 ± 0.466
9.037AlaGly: 9.037 ± 1.031
1.643AlaHis: 1.643 ± 0.329
6.299AlaIle: 6.299 ± 0.633
3.971AlaLys: 3.971 ± 0.61
12.666AlaLeu: 12.666 ± 1.072
4.245AlaMet: 4.245 ± 0.492
3.081AlaAsn: 3.081 ± 0.511
5.203AlaPro: 5.203 ± 0.611
6.23AlaGln: 6.23 ± 0.761
8.695AlaArg: 8.695 ± 0.937
6.915AlaSer: 6.915 ± 0.836
5.751AlaThr: 5.751 ± 0.627
6.915AlaVal: 6.915 ± 0.733
1.849AlaTrp: 1.849 ± 0.251
2.944AlaTyr: 2.944 ± 0.435
0.0AlaXaa: 0.0 ± 0.0
Cys
0.959CysAla: 0.959 ± 0.299
0.068CysCys: 0.068 ± 0.075
0.822CysAsp: 0.822 ± 0.212
0.616CysGlu: 0.616 ± 0.248
0.479CysPhe: 0.479 ± 0.19
0.616CysGly: 0.616 ± 0.251
0.411CysHis: 0.411 ± 0.191
0.137CysIle: 0.137 ± 0.116
0.068CysLys: 0.068 ± 0.062
0.411CysLeu: 0.411 ± 0.23
0.205CysMet: 0.205 ± 0.133
0.205CysAsn: 0.205 ± 0.113
0.685CysPro: 0.685 ± 0.212
0.137CysGln: 0.137 ± 0.109
1.027CysArg: 1.027 ± 0.321
0.685CysSer: 0.685 ± 0.238
0.479CysThr: 0.479 ± 0.17
0.342CysVal: 0.342 ± 0.147
0.205CysTrp: 0.205 ± 0.122
0.274CysTyr: 0.274 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
6.162AspAla: 6.162 ± 0.679
0.342AspCys: 0.342 ± 0.172
3.149AspAsp: 3.149 ± 0.508
4.382AspGlu: 4.382 ± 0.561
1.712AspPhe: 1.712 ± 0.291
6.367AspGly: 6.367 ± 0.779
1.232AspHis: 1.232 ± 0.291
1.917AspIle: 1.917 ± 0.357
1.301AspLys: 1.301 ± 0.339
5.751AspLeu: 5.751 ± 0.652
1.301AspMet: 1.301 ± 0.379
1.438AspAsn: 1.438 ± 0.28
2.807AspPro: 2.807 ± 0.533
3.218AspGln: 3.218 ± 0.664
3.355AspArg: 3.355 ± 0.408
3.355AspSer: 3.355 ± 0.522
2.944AspThr: 2.944 ± 0.519
3.492AspVal: 3.492 ± 0.541
1.095AspTrp: 1.095 ± 0.23
1.506AspTyr: 1.506 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
6.641GluAla: 6.641 ± 0.723
0.548GluCys: 0.548 ± 0.243
2.944GluAsp: 2.944 ± 0.597
3.423GluGlu: 3.423 ± 0.624
2.191GluPhe: 2.191 ± 0.471
3.766GluGly: 3.766 ± 0.567
0.89GluHis: 0.89 ± 0.292
2.67GluIle: 2.67 ± 0.428
2.259GluLys: 2.259 ± 0.415
6.915GluLeu: 6.915 ± 0.729
1.438GluMet: 1.438 ± 0.433
1.164GluAsn: 1.164 ± 0.28
2.944GluPro: 2.944 ± 0.424
4.176GluGln: 4.176 ± 0.56
4.861GluArg: 4.861 ± 0.628
2.739GluSer: 2.739 ± 0.343
3.012GluThr: 3.012 ± 0.45
4.998GluVal: 4.998 ± 0.559
0.822GluTrp: 0.822 ± 0.261
1.438GluTyr: 1.438 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
3.355PheAla: 3.355 ± 0.491
0.274PheCys: 0.274 ± 0.119
1.917PheAsp: 1.917 ± 0.351
1.438PheGlu: 1.438 ± 0.356
0.959PhePhe: 0.959 ± 0.281
2.465PheGly: 2.465 ± 0.378
0.616PheHis: 0.616 ± 0.2
1.027PheIle: 1.027 ± 0.285
1.232PheLys: 1.232 ± 0.329
2.054PheLeu: 2.054 ± 0.396
0.753PheMet: 0.753 ± 0.287
0.959PheAsn: 0.959 ± 0.336
1.575PhePro: 1.575 ± 0.341
1.643PheGln: 1.643 ± 0.303
2.054PheArg: 2.054 ± 0.432
1.712PheSer: 1.712 ± 0.311
1.575PheThr: 1.575 ± 0.284
1.369PheVal: 1.369 ± 0.266
0.274PheTrp: 0.274 ± 0.135
0.822PheTyr: 0.822 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
7.531GlyAla: 7.531 ± 1.043
0.479GlyCys: 0.479 ± 0.177
4.45GlyAsp: 4.45 ± 0.623
4.382GlyGlu: 4.382 ± 0.548
2.807GlyPhe: 2.807 ± 0.345
6.025GlyGly: 6.025 ± 0.754
0.959GlyHis: 0.959 ± 0.252
3.971GlyIle: 3.971 ± 0.497
3.355GlyLys: 3.355 ± 0.518
8.284GlyLeu: 8.284 ± 0.803
1.438GlyMet: 1.438 ± 0.324
2.122GlyAsn: 2.122 ± 0.499
2.944GlyPro: 2.944 ± 0.421
4.724GlyGln: 4.724 ± 0.595
7.189GlyArg: 7.189 ± 0.733
4.998GlySer: 4.998 ± 0.605
4.382GlyThr: 4.382 ± 0.529
4.998GlyVal: 4.998 ± 0.592
1.849GlyTrp: 1.849 ± 0.384
2.328GlyTyr: 2.328 ± 0.573
0.0GlyXaa: 0.0 ± 0.0
His
1.917HisAla: 1.917 ± 0.351
0.205HisCys: 0.205 ± 0.106
0.616HisAsp: 0.616 ± 0.175
0.959HisGlu: 0.959 ± 0.344
0.616HisPhe: 0.616 ± 0.204
1.301HisGly: 1.301 ± 0.297
0.342HisHis: 0.342 ± 0.233
0.479HisIle: 0.479 ± 0.171
0.205HisLys: 0.205 ± 0.117
2.122HisLeu: 2.122 ± 0.432
0.616HisMet: 0.616 ± 0.197
0.753HisAsn: 0.753 ± 0.237
1.232HisPro: 1.232 ± 0.285
0.89HisGln: 0.89 ± 0.332
1.164HisArg: 1.164 ± 0.327
0.685HisSer: 0.685 ± 0.283
0.959HisThr: 0.959 ± 0.316
1.095HisVal: 1.095 ± 0.263
0.411HisTrp: 0.411 ± 0.205
0.822HisTyr: 0.822 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.861IleAla: 4.861 ± 0.641
0.548IleCys: 0.548 ± 0.217
3.286IleAsp: 3.286 ± 0.393
3.56IleGlu: 3.56 ± 0.709
0.89IlePhe: 0.89 ± 0.232
3.697IleGly: 3.697 ± 0.409
0.753IleHis: 0.753 ± 0.193
1.369IleIle: 1.369 ± 0.319
1.232IleLys: 1.232 ± 0.263
2.807IleLeu: 2.807 ± 0.324
0.685IleMet: 0.685 ± 0.264
1.095IleAsn: 1.095 ± 0.308
2.328IlePro: 2.328 ± 0.346
2.122IleGln: 2.122 ± 0.482
3.697IleArg: 3.697 ± 0.497
2.122IleSer: 2.122 ± 0.41
2.396IleThr: 2.396 ± 0.531
2.67IleVal: 2.67 ± 0.339
0.616IleTrp: 0.616 ± 0.297
1.164IleTyr: 1.164 ± 0.318
0.0IleXaa: 0.0 ± 0.0
Lys
4.998LysAla: 4.998 ± 0.75
0.0LysCys: 0.0 ± 0.0
1.575LysAsp: 1.575 ± 0.343
1.643LysGlu: 1.643 ± 0.344
0.548LysPhe: 0.548 ± 0.207
2.465LysGly: 2.465 ± 0.37
0.548LysHis: 0.548 ± 0.206
1.232LysIle: 1.232 ± 0.364
1.78LysLys: 1.78 ± 0.384
3.218LysLeu: 3.218 ± 0.492
0.822LysMet: 0.822 ± 0.339
1.027LysAsn: 1.027 ± 0.236
2.396LysPro: 2.396 ± 0.54
1.438LysGln: 1.438 ± 0.296
2.533LysArg: 2.533 ± 0.434
1.985LysSer: 1.985 ± 0.443
1.917LysThr: 1.917 ± 0.341
2.465LysVal: 2.465 ± 0.398
0.411LysTrp: 0.411 ± 0.139
0.89LysTyr: 0.89 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
13.214LeuAla: 13.214 ± 1.208
0.822LeuCys: 0.822 ± 0.209
6.573LeuAsp: 6.573 ± 0.747
6.367LeuGlu: 6.367 ± 0.741
2.396LeuPhe: 2.396 ± 0.397
8.832LeuGly: 8.832 ± 0.806
2.396LeuHis: 2.396 ± 0.405
3.218LeuIle: 3.218 ± 0.514
3.355LeuLys: 3.355 ± 0.708
9.722LeuLeu: 9.722 ± 0.857
2.328LeuMet: 2.328 ± 0.416
3.081LeuAsn: 3.081 ± 0.498
5.066LeuPro: 5.066 ± 0.673
4.313LeuGln: 4.313 ± 0.66
7.257LeuArg: 7.257 ± 0.907
5.203LeuSer: 5.203 ± 0.568
5.614LeuThr: 5.614 ± 0.715
6.847LeuVal: 6.847 ± 0.678
1.301LeuTrp: 1.301 ± 0.261
2.465LeuTyr: 2.465 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
3.56MetAla: 3.56 ± 0.443
0.068MetCys: 0.068 ± 0.066
1.917MetAsp: 1.917 ± 0.36
1.232MetGlu: 1.232 ± 0.328
0.342MetPhe: 0.342 ± 0.157
1.164MetGly: 1.164 ± 0.219
0.274MetHis: 0.274 ± 0.127
0.548MetIle: 0.548 ± 0.162
0.822MetLys: 0.822 ± 0.251
2.122MetLeu: 2.122 ± 0.355
0.342MetMet: 0.342 ± 0.164
0.822MetAsn: 0.822 ± 0.252
1.643MetPro: 1.643 ± 0.453
1.301MetGln: 1.301 ± 0.297
1.506MetArg: 1.506 ± 0.301
2.054MetSer: 2.054 ± 0.371
1.438MetThr: 1.438 ± 0.293
0.959MetVal: 0.959 ± 0.263
0.274MetTrp: 0.274 ± 0.187
0.274MetTyr: 0.274 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.286AsnAla: 3.286 ± 0.622
0.068AsnCys: 0.068 ± 0.073
1.369AsnAsp: 1.369 ± 0.276
1.095AsnGlu: 1.095 ± 0.316
0.685AsnPhe: 0.685 ± 0.186
2.602AsnGly: 2.602 ± 0.56
0.548AsnHis: 0.548 ± 0.185
1.027AsnIle: 1.027 ± 0.279
1.301AsnLys: 1.301 ± 0.349
2.876AsnLeu: 2.876 ± 0.509
0.685AsnMet: 0.685 ± 0.214
1.232AsnAsn: 1.232 ± 0.33
2.465AsnPro: 2.465 ± 0.461
0.89AsnGln: 0.89 ± 0.259
2.876AsnArg: 2.876 ± 0.43
1.164AsnSer: 1.164 ± 0.333
1.301AsnThr: 1.301 ± 0.301
0.822AsnVal: 0.822 ± 0.23
0.548AsnTrp: 0.548 ± 0.163
0.959AsnTyr: 0.959 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
6.71ProAla: 6.71 ± 0.628
0.685ProCys: 0.685 ± 0.215
3.423ProAsp: 3.423 ± 0.507
2.876ProGlu: 2.876 ± 0.408
1.78ProPhe: 1.78 ± 0.424
4.519ProGly: 4.519 ± 0.497
0.89ProHis: 0.89 ± 0.288
1.849ProIle: 1.849 ± 0.374
1.849ProLys: 1.849 ± 0.374
4.45ProLeu: 4.45 ± 0.501
1.027ProMet: 1.027 ± 0.278
1.575ProAsn: 1.575 ± 0.338
2.807ProPro: 2.807 ± 0.462
2.944ProGln: 2.944 ± 0.49
3.766ProArg: 3.766 ± 0.523
3.56ProSer: 3.56 ± 0.506
2.67ProThr: 2.67 ± 0.464
3.629ProVal: 3.629 ± 0.66
0.479ProTrp: 0.479 ± 0.185
1.369ProTyr: 1.369 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
6.915GlnAla: 6.915 ± 0.845
0.411GlnCys: 0.411 ± 0.221
2.328GlnAsp: 2.328 ± 0.431
2.054GlnGlu: 2.054 ± 0.439
1.095GlnPhe: 1.095 ± 0.353
3.766GlnGly: 3.766 ± 0.534
1.095GlnHis: 1.095 ± 0.303
2.259GlnIle: 2.259 ± 0.368
1.369GlnLys: 1.369 ± 0.324
6.641GlnLeu: 6.641 ± 0.723
1.301GlnMet: 1.301 ± 0.303
1.027GlnAsn: 1.027 ± 0.263
2.328GlnPro: 2.328 ± 0.327
3.903GlnGln: 3.903 ± 0.813
4.793GlnArg: 4.793 ± 0.682
2.533GlnSer: 2.533 ± 0.372
2.465GlnThr: 2.465 ± 0.368
4.519GlnVal: 4.519 ± 0.448
0.685GlnTrp: 0.685 ± 0.246
0.89GlnTyr: 0.89 ± 0.252
0.0GlnXaa: 0.0 ± 0.0
Arg
7.873ArgAla: 7.873 ± 0.75
0.753ArgCys: 0.753 ± 0.202
4.039ArgAsp: 4.039 ± 0.562
4.724ArgGlu: 4.724 ± 0.52
2.122ArgPhe: 2.122 ± 0.414
4.724ArgGly: 4.724 ± 0.598
1.712ArgHis: 1.712 ± 0.363
3.834ArgIle: 3.834 ± 0.492
2.259ArgLys: 2.259 ± 0.405
8.627ArgLeu: 8.627 ± 0.918
1.095ArgMet: 1.095 ± 0.205
1.712ArgAsn: 1.712 ± 0.327
4.45ArgPro: 4.45 ± 0.828
4.176ArgGln: 4.176 ± 0.583
6.162ArgArg: 6.162 ± 0.816
4.313ArgSer: 4.313 ± 0.493
2.944ArgThr: 2.944 ± 0.356
5.34ArgVal: 5.34 ± 0.646
1.438ArgTrp: 1.438 ± 0.344
2.533ArgTyr: 2.533 ± 0.419
0.0ArgXaa: 0.0 ± 0.0
Ser
7.326SerAla: 7.326 ± 0.823
0.479SerCys: 0.479 ± 0.168
3.629SerAsp: 3.629 ± 0.643
2.465SerGlu: 2.465 ± 0.37
1.095SerPhe: 1.095 ± 0.251
5.066SerGly: 5.066 ± 0.609
0.753SerHis: 0.753 ± 0.228
3.149SerIle: 3.149 ± 0.493
1.985SerLys: 1.985 ± 0.399
5.888SerLeu: 5.888 ± 0.599
0.89SerMet: 0.89 ± 0.268
2.054SerAsn: 2.054 ± 0.511
3.492SerPro: 3.492 ± 0.601
3.218SerGln: 3.218 ± 0.528
3.081SerArg: 3.081 ± 0.488
3.971SerSer: 3.971 ± 0.555
2.944SerThr: 2.944 ± 0.586
3.286SerVal: 3.286 ± 0.466
1.369SerTrp: 1.369 ± 0.285
1.78SerTyr: 1.78 ± 0.354
0.0SerXaa: 0.0 ± 0.0
Thr
6.436ThrAla: 6.436 ± 0.842
0.479ThrCys: 0.479 ± 0.237
3.012ThrAsp: 3.012 ± 0.435
3.012ThrGlu: 3.012 ± 0.488
1.438ThrPhe: 1.438 ± 0.34
4.382ThrGly: 4.382 ± 0.613
0.89ThrHis: 0.89 ± 0.342
1.985ThrIle: 1.985 ± 0.394
1.849ThrLys: 1.849 ± 0.432
5.203ThrLeu: 5.203 ± 0.75
0.548ThrMet: 0.548 ± 0.214
1.301ThrAsn: 1.301 ± 0.283
3.081ThrPro: 3.081 ± 0.334
1.575ThrGln: 1.575 ± 0.308
2.807ThrArg: 2.807 ± 0.392
3.423ThrSer: 3.423 ± 0.458
2.876ThrThr: 2.876 ± 0.532
5.34ThrVal: 5.34 ± 0.685
0.822ThrTrp: 0.822 ± 0.218
1.301ThrTyr: 1.301 ± 0.299
0.0ThrXaa: 0.0 ± 0.0
Val
8.9ValAla: 8.9 ± 0.741
0.548ValCys: 0.548 ± 0.189
3.56ValAsp: 3.56 ± 0.49
4.793ValGlu: 4.793 ± 0.728
1.643ValPhe: 1.643 ± 0.322
5.203ValGly: 5.203 ± 0.704
0.616ValHis: 0.616 ± 0.178
2.807ValIle: 2.807 ± 0.382
2.465ValLys: 2.465 ± 0.372
6.23ValLeu: 6.23 ± 0.637
1.78ValMet: 1.78 ± 0.398
1.849ValAsn: 1.849 ± 0.3
3.081ValPro: 3.081 ± 0.483
3.012ValGln: 3.012 ± 0.461
4.724ValArg: 4.724 ± 0.593
3.766ValSer: 3.766 ± 0.453
4.176ValThr: 4.176 ± 0.591
4.656ValVal: 4.656 ± 0.635
0.959ValTrp: 0.959 ± 0.207
2.465ValTyr: 2.465 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.506TrpAla: 1.506 ± 0.239
0.342TrpCys: 0.342 ± 0.152
0.616TrpAsp: 0.616 ± 0.209
0.753TrpGlu: 0.753 ± 0.27
0.548TrpPhe: 0.548 ± 0.165
1.027TrpGly: 1.027 ± 0.332
0.137TrpHis: 0.137 ± 0.116
0.822TrpIle: 0.822 ± 0.246
0.685TrpLys: 0.685 ± 0.188
2.191TrpLeu: 2.191 ± 0.391
0.685TrpMet: 0.685 ± 0.22
0.342TrpAsn: 0.342 ± 0.163
0.959TrpPro: 0.959 ± 0.299
0.959TrpGln: 0.959 ± 0.245
1.232TrpArg: 1.232 ± 0.348
0.822TrpSer: 0.822 ± 0.229
0.753TrpThr: 0.753 ± 0.25
1.438TrpVal: 1.438 ± 0.265
0.479TrpTrp: 0.479 ± 0.139
0.137TrpTyr: 0.137 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.492TyrAla: 3.492 ± 0.475
0.342TyrCys: 0.342 ± 0.146
1.027TyrAsp: 1.027 ± 0.267
1.712TyrGlu: 1.712 ± 0.33
0.822TyrPhe: 0.822 ± 0.229
2.191TyrGly: 2.191 ± 0.367
0.685TyrHis: 0.685 ± 0.3
1.301TyrIle: 1.301 ± 0.286
0.548TyrLys: 0.548 ± 0.216
2.259TyrLeu: 2.259 ± 0.388
0.411TyrMet: 0.411 ± 0.166
0.959TyrAsn: 0.959 ± 0.269
1.369TyrPro: 1.369 ± 0.427
1.506TyrGln: 1.506 ± 0.329
2.054TyrArg: 2.054 ± 0.382
1.917TyrSer: 1.917 ± 0.413
1.164TyrThr: 1.164 ± 0.356
2.054TyrVal: 2.054 ± 0.342
0.548TyrTrp: 0.548 ± 0.205
0.479TyrTyr: 0.479 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (14607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski