Amino acid dipepetide frequency for Synechococcus sp. RS9916

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.49AlaAla: 13.49 ± 0.265
1.453AlaCys: 1.453 ± 0.055
6.052AlaAsp: 6.052 ± 0.111
6.03AlaGlu: 6.03 ± 0.141
3.146AlaPhe: 3.146 ± 0.073
9.15AlaGly: 9.15 ± 0.277
1.775AlaHis: 1.775 ± 0.061
5.041AlaIle: 5.041 ± 0.097
2.936AlaLys: 2.936 ± 0.081
13.505AlaLeu: 13.505 ± 0.22
2.848AlaMet: 2.848 ± 0.078
2.853AlaAsn: 2.853 ± 0.138
4.928AlaPro: 4.928 ± 0.104
4.479AlaGln: 4.479 ± 0.126
5.955AlaArg: 5.955 ± 0.14
7.041AlaSer: 7.041 ± 0.173
5.184AlaThr: 5.184 ± 0.106
7.979AlaVal: 7.979 ± 0.156
1.657AlaTrp: 1.657 ± 0.053
2.058AlaTyr: 2.058 ± 0.145
0.0AlaXaa: 0.0 ± 0.0
Cys
0.906CysAla: 0.906 ± 0.033
0.239CysCys: 0.239 ± 0.018
0.711CysAsp: 0.711 ± 0.034
0.589CysGlu: 0.589 ± 0.027
0.438CysPhe: 0.438 ± 0.025
1.217CysGly: 1.217 ± 0.045
0.302CysHis: 0.302 ± 0.02
0.462CysIle: 0.462 ± 0.025
0.284CysLys: 0.284 ± 0.02
1.345CysLeu: 1.345 ± 0.055
0.237CysMet: 0.237 ± 0.02
0.332CysAsn: 0.332 ± 0.021
0.67CysPro: 0.67 ± 0.032
0.462CysGln: 0.462 ± 0.022
0.989CysArg: 0.989 ± 0.041
0.916CysSer: 0.916 ± 0.043
0.529CysThr: 0.529 ± 0.028
0.718CysVal: 0.718 ± 0.034
0.279CysTrp: 0.279 ± 0.02
0.221CysTyr: 0.221 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
5.73AspAla: 5.73 ± 0.097
0.617AspCys: 0.617 ± 0.029
2.838AspAsp: 2.838 ± 0.082
2.712AspGlu: 2.712 ± 0.076
1.546AspPhe: 1.546 ± 0.045
5.284AspGly: 5.284 ± 0.173
1.326AspHis: 1.326 ± 0.048
1.665AspIle: 1.665 ± 0.057
1.149AspLys: 1.149 ± 0.049
7.12AspLeu: 7.12 ± 0.126
0.823AspMet: 0.823 ± 0.032
1.356AspAsn: 1.356 ± 0.055
3.854AspPro: 3.854 ± 0.11
3.266AspGln: 3.266 ± 0.082
3.74AspArg: 3.74 ± 0.105
2.859AspSer: 2.859 ± 0.065
2.214AspThr: 2.214 ± 0.123
3.41AspVal: 3.41 ± 0.076
0.989AspTrp: 0.989 ± 0.041
1.129AspTyr: 1.129 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
7.141GluAla: 7.141 ± 0.17
0.404GluCys: 0.404 ± 0.022
2.311GluAsp: 2.311 ± 0.071
3.137GluGlu: 3.137 ± 0.096
1.219GluPhe: 1.219 ± 0.044
4.403GluGly: 4.403 ± 0.081
1.185GluHis: 1.185 ± 0.045
2.15GluIle: 2.15 ± 0.064
1.519GluLys: 1.519 ± 0.058
6.999GluLeu: 6.999 ± 0.165
1.005GluMet: 1.005 ± 0.041
1.154GluAsn: 1.154 ± 0.043
2.824GluPro: 2.824 ± 0.086
3.652GluGln: 3.652 ± 0.098
4.921GluArg: 4.921 ± 0.123
2.866GluSer: 2.866 ± 0.077
2.673GluThr: 2.673 ± 0.073
3.666GluVal: 3.666 ± 0.097
0.701GluTrp: 0.701 ± 0.033
0.646GluTyr: 0.646 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
2.97PheAla: 2.97 ± 0.066
0.508PheCys: 0.508 ± 0.028
1.895PheAsp: 1.895 ± 0.061
1.624PheGlu: 1.624 ± 0.054
1.029PhePhe: 1.029 ± 0.042
2.773PheGly: 2.773 ± 0.077
0.614PheHis: 0.614 ± 0.031
1.13PheIle: 1.13 ± 0.041
0.8PheLys: 0.8 ± 0.039
2.996PheLeu: 2.996 ± 0.069
0.559PheMet: 0.559 ± 0.027
1.151PheAsn: 1.151 ± 0.042
1.346PhePro: 1.346 ± 0.042
1.217PheGln: 1.217 ± 0.035
1.812PheArg: 1.812 ± 0.056
2.369PheSer: 2.369 ± 0.057
1.699PheThr: 1.699 ± 0.058
1.837PheVal: 1.837 ± 0.054
0.54PheTrp: 0.54 ± 0.025
0.7PheTyr: 0.7 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
7.928GlyAla: 7.928 ± 0.257
1.264GlyCys: 1.264 ± 0.047
4.927GlyAsp: 4.927 ± 0.181
4.35GlyGlu: 4.35 ± 0.088
3.092GlyPhe: 3.092 ± 0.069
9.821GlyGly: 9.821 ± 1.576
1.794GlyHis: 1.794 ± 0.055
4.045GlyIle: 4.045 ± 0.124
2.516GlyLys: 2.516 ± 0.073
10.433GlyLeu: 10.433 ± 0.139
2.027GlyMet: 2.027 ± 0.058
2.779GlyAsn: 2.779 ± 0.314
3.224GlyPro: 3.224 ± 0.087
3.654GlyGln: 3.654 ± 0.073
5.572GlyArg: 5.572 ± 0.109
6.585GlySer: 6.585 ± 0.45
4.552GlyThr: 4.552 ± 0.206
6.035GlyVal: 6.035 ± 0.108
1.837GlyTrp: 1.837 ± 0.062
1.865GlyTyr: 1.865 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.8HisAla: 1.8 ± 0.059
0.408HisCys: 0.408 ± 0.029
0.995HisAsp: 0.995 ± 0.041
0.798HisGlu: 0.798 ± 0.036
0.682HisPhe: 0.682 ± 0.037
1.927HisGly: 1.927 ± 0.064
0.828HisHis: 0.828 ± 0.042
0.574HisIle: 0.574 ± 0.027
0.387HisLys: 0.387 ± 0.025
2.496HisLeu: 2.496 ± 0.08
0.256HisMet: 0.256 ± 0.018
0.59HisAsn: 0.59 ± 0.029
1.6HisPro: 1.6 ± 0.065
1.217HisGln: 1.217 ± 0.051
1.716HisArg: 1.716 ± 0.053
1.229HisSer: 1.229 ± 0.046
0.907HisThr: 0.907 ± 0.04
1.023HisVal: 1.023 ± 0.039
0.631HisTrp: 0.631 ± 0.03
0.509HisTyr: 0.509 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
4.749IleAla: 4.749 ± 0.095
0.505IleCys: 0.505 ± 0.026
2.92IleAsp: 2.92 ± 0.069
2.535IleGlu: 2.535 ± 0.072
0.948IlePhe: 0.948 ± 0.045
4.406IleGly: 4.406 ± 0.25
0.949IleHis: 0.949 ± 0.037
1.149IleIle: 1.149 ± 0.046
1.32IleLys: 1.32 ± 0.047
3.132IleLeu: 3.132 ± 0.072
0.358IleMet: 0.358 ± 0.023
1.784IleAsn: 1.784 ± 0.096
2.351IlePro: 2.351 ± 0.07
1.852IleGln: 1.852 ± 0.063
2.639IleArg: 2.639 ± 0.075
3.097IleSer: 3.097 ± 0.114
3.195IleThr: 3.195 ± 0.186
2.392IleVal: 2.392 ± 0.063
0.641IleTrp: 0.641 ± 0.033
0.849IleTyr: 0.849 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.49LysAla: 3.49 ± 0.08
0.19LysCys: 0.19 ± 0.014
1.42LysAsp: 1.42 ± 0.048
1.609LysGlu: 1.609 ± 0.059
0.609LysPhe: 0.609 ± 0.032
2.477LysGly: 2.477 ± 0.069
0.453LysHis: 0.453 ± 0.023
0.954LysIle: 0.954 ± 0.037
0.988LysLys: 0.988 ± 0.043
2.754LysLeu: 2.754 ± 0.077
0.41LysMet: 0.41 ± 0.027
0.722LysAsn: 0.722 ± 0.034
1.815LysPro: 1.815 ± 0.058
1.393LysGln: 1.393 ± 0.051
2.094LysArg: 2.094 ± 0.067
1.541LysSer: 1.541 ± 0.052
1.577LysThr: 1.577 ± 0.052
1.941LysVal: 1.941 ± 0.063
0.246LysTrp: 0.246 ± 0.018
0.438LysTyr: 0.438 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
12.942LeuAla: 12.942 ± 0.243
1.244LeuCys: 1.244 ± 0.048
6.38LeuAsp: 6.38 ± 0.135
7.821LeuGlu: 7.821 ± 0.194
3.173LeuPhe: 3.173 ± 0.079
9.373LeuGly: 9.373 ± 0.158
2.349LeuHis: 2.349 ± 0.07
5.452LeuIle: 5.452 ± 0.082
3.665LeuLys: 3.665 ± 0.094
14.66LeuLeu: 14.66 ± 0.289
2.642LeuMet: 2.642 ± 0.072
3.715LeuAsn: 3.715 ± 0.22
6.741LeuPro: 6.741 ± 0.173
6.443LeuGln: 6.443 ± 0.133
8.052LeuArg: 8.052 ± 0.169
7.665LeuSer: 7.665 ± 0.13
6.101LeuThr: 6.101 ± 0.16
8.777LeuVal: 8.777 ± 0.173
1.71LeuTrp: 1.71 ± 0.06
1.726LeuTyr: 1.726 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
3.046MetAla: 3.046 ± 0.085
0.114MetCys: 0.114 ± 0.013
0.927MetAsp: 0.927 ± 0.041
1.174MetGlu: 1.174 ± 0.048
0.402MetPhe: 0.402 ± 0.024
1.889MetGly: 1.889 ± 0.053
0.383MetHis: 0.383 ± 0.025
0.699MetIle: 0.699 ± 0.03
0.591MetLys: 0.591 ± 0.031
2.187MetLeu: 2.187 ± 0.062
0.253MetMet: 0.253 ± 0.02
0.519MetAsn: 0.519 ± 0.026
1.318MetPro: 1.318 ± 0.047
0.918MetGln: 0.918 ± 0.033
1.332MetArg: 1.332 ± 0.048
1.222MetSer: 1.222 ± 0.041
1.372MetThr: 1.372 ± 0.05
1.691MetVal: 1.691 ± 0.054
0.114MetTrp: 0.114 ± 0.014
0.206MetTyr: 0.206 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.337AsnAla: 3.337 ± 0.212
0.291AsnCys: 0.291 ± 0.02
1.597AsnAsp: 1.597 ± 0.056
1.249AsnGlu: 1.249 ± 0.101
0.883AsnPhe: 0.883 ± 0.039
2.679AsnGly: 2.679 ± 0.096
0.584AsnHis: 0.584 ± 0.028
1.022AsnIle: 1.022 ± 0.071
0.742AsnLys: 0.742 ± 0.039
3.253AsnLeu: 3.253 ± 0.104
0.379AsnMet: 0.379 ± 0.025
1.089AsnAsn: 1.089 ± 0.078
1.943AsnPro: 1.943 ± 0.05
1.305AsnGln: 1.305 ± 0.05
2.049AsnArg: 2.049 ± 0.051
2.021AsnSer: 2.021 ± 0.18
2.023AsnThr: 2.023 ± 0.411
1.599AsnVal: 1.599 ± 0.092
0.504AsnTrp: 0.504 ± 0.029
0.58AsnTyr: 0.58 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
5.804ProAla: 5.804 ± 0.113
0.556ProCys: 0.556 ± 0.032
3.274ProAsp: 3.274 ± 0.079
3.737ProGlu: 3.737 ± 0.107
1.723ProPhe: 1.723 ± 0.053
4.517ProGly: 4.517 ± 0.101
1.004ProHis: 1.004 ± 0.045
2.099ProIle: 2.099 ± 0.056
1.421ProLys: 1.421 ± 0.051
6.664ProLeu: 6.664 ± 0.163
1.261ProMet: 1.261 ± 0.045
1.295ProAsn: 1.295 ± 0.043
2.628ProPro: 2.628 ± 0.093
2.346ProGln: 2.346 ± 0.067
2.819ProArg: 2.819 ± 0.082
3.787ProSer: 3.787 ± 0.094
2.644ProThr: 2.644 ± 0.079
4.131ProVal: 4.131 ± 0.102
1.048ProTrp: 1.048 ± 0.046
0.955ProTyr: 0.955 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
5.646GlnAla: 5.646 ± 0.109
0.444GlnCys: 0.444 ± 0.025
1.882GlnAsp: 1.882 ± 0.062
2.497GlnGlu: 2.497 ± 0.076
1.095GlnPhe: 1.095 ± 0.041
3.854GlnGly: 3.854 ± 0.089
1.009GlnHis: 1.009 ± 0.044
1.749GlnIle: 1.749 ± 0.047
1.184GlnLys: 1.184 ± 0.047
6.726GlnLeu: 6.726 ± 0.145
0.856GlnMet: 0.856 ± 0.034
0.942GlnAsn: 0.942 ± 0.034
2.924GlnPro: 2.924 ± 0.086
3.4GlnGln: 3.4 ± 0.116
4.365GlnArg: 4.365 ± 0.124
2.991GlnSer: 2.991 ± 0.232
2.258GlnThr: 2.258 ± 0.07
3.415GlnVal: 3.415 ± 0.085
0.913GlnTrp: 0.913 ± 0.041
0.529GlnTyr: 0.529 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
5.348ArgAla: 5.348 ± 0.103
1.043ArgCys: 1.043 ± 0.042
3.519ArgAsp: 3.519 ± 0.085
3.533ArgGlu: 3.533 ± 0.096
2.78ArgPhe: 2.78 ± 0.08
4.476ArgGly: 4.476 ± 0.102
1.715ArgHis: 1.715 ± 0.069
3.071ArgIle: 3.071 ± 0.067
1.73ArgLys: 1.73 ± 0.064
9.047ArgLeu: 9.047 ± 0.212
1.59ArgMet: 1.59 ± 0.052
1.849ArgAsn: 1.849 ± 0.057
3.452ArgPro: 3.452 ± 0.111
3.968ArgGln: 3.968 ± 0.118
5.672ArgArg: 5.672 ± 0.147
4.595ArgSer: 4.595 ± 0.105
2.879ArgThr: 2.879 ± 0.08
4.139ArgVal: 4.139 ± 0.093
1.76ArgTrp: 1.76 ± 0.061
1.486ArgTyr: 1.486 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
6.545SerAla: 6.545 ± 0.184
0.801SerCys: 0.801 ± 0.033
3.691SerAsp: 3.691 ± 0.153
3.121SerGlu: 3.121 ± 0.066
2.047SerPhe: 2.047 ± 0.058
6.737SerGly: 6.737 ± 0.372
1.37SerHis: 1.37 ± 0.047
3.117SerIle: 3.117 ± 0.25
1.728SerLys: 1.728 ± 0.052
7.456SerLeu: 7.456 ± 0.121
1.415SerMet: 1.415 ± 0.043
2.173SerAsn: 2.173 ± 0.138
3.439SerPro: 3.439 ± 0.079
2.644SerGln: 2.644 ± 0.055
4.111SerArg: 4.111 ± 0.107
5.248SerSer: 5.248 ± 0.216
3.937SerThr: 3.937 ± 0.167
4.524SerVal: 4.524 ± 0.208
1.235SerTrp: 1.235 ± 0.042
1.331SerTyr: 1.331 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
6.066ThrAla: 6.066 ± 0.193
0.5ThrCys: 0.5 ± 0.031
2.515ThrAsp: 2.515 ± 0.073
2.365ThrGlu: 2.365 ± 0.065
1.499ThrPhe: 1.499 ± 0.055
4.85ThrGly: 4.85 ± 0.229
0.929ThrHis: 0.929 ± 0.037
2.48ThrIle: 2.48 ± 0.115
1.271ThrLys: 1.271 ± 0.05
6.525ThrLeu: 6.525 ± 0.451
0.921ThrMet: 0.921 ± 0.038
1.55ThrAsn: 1.55 ± 0.108
3.475ThrPro: 3.475 ± 0.088
1.76ThrGln: 1.76 ± 0.05
2.739ThrArg: 2.739 ± 0.075
3.767ThrSer: 3.767 ± 0.223
3.677ThrThr: 3.677 ± 0.249
4.024ThrVal: 4.024 ± 0.182
0.746ThrTrp: 0.746 ± 0.033
1.139ThrTyr: 1.139 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
7.524ValAla: 7.524 ± 0.116
0.75ValCys: 0.75 ± 0.038
3.901ValAsp: 3.901 ± 0.09
4.136ValGlu: 4.136 ± 0.11
2.122ValPhe: 2.122 ± 0.056
5.309ValGly: 5.309 ± 0.133
1.31ValHis: 1.31 ± 0.051
3.454ValIle: 3.454 ± 0.077
1.926ValLys: 1.926 ± 0.067
8.807ValLeu: 8.807 ± 0.153
1.73ValMet: 1.73 ± 0.056
2.247ValAsn: 2.247 ± 0.105
3.312ValPro: 3.312 ± 0.083
2.886ValGln: 2.886 ± 0.068
4.106ValArg: 4.106 ± 0.107
4.407ValSer: 4.407 ± 0.123
3.738ValThr: 3.738 ± 0.17
6.536ValVal: 6.536 ± 0.126
0.863ValTrp: 0.863 ± 0.035
1.065ValTyr: 1.065 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.3TrpAla: 1.3 ± 0.049
0.254TrpCys: 0.254 ± 0.017
0.831TrpAsp: 0.831 ± 0.039
0.704TrpGlu: 0.704 ± 0.031
0.579TrpPhe: 0.579 ± 0.031
1.22TrpGly: 1.22 ± 0.041
0.392TrpHis: 0.392 ± 0.023
0.879TrpIle: 0.879 ± 0.034
0.442TrpLys: 0.442 ± 0.026
2.566TrpLeu: 2.566 ± 0.075
0.508TrpMet: 0.508 ± 0.026
0.49TrpAsn: 0.49 ± 0.032
0.907TrpPro: 0.907 ± 0.043
1.029TrpGln: 1.029 ± 0.042
1.412TrpArg: 1.412 ± 0.05
1.17TrpSer: 1.17 ± 0.041
0.776TrpThr: 0.776 ± 0.034
1.083TrpVal: 1.083 ± 0.04
0.439TrpTrp: 0.439 ± 0.032
0.235TrpTyr: 0.235 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.8TyrAla: 1.8 ± 0.082
0.261TyrCys: 0.261 ± 0.02
1.042TyrAsp: 1.042 ± 0.043
0.859TyrGlu: 0.859 ± 0.04
0.558TyrPhe: 0.558 ± 0.028
1.938TyrGly: 1.938 ± 0.058
0.304TyrHis: 0.304 ± 0.025
0.58TyrIle: 0.58 ± 0.029
0.523TyrLys: 0.523 ± 0.028
1.96TyrLeu: 1.96 ± 0.063
0.277TyrMet: 0.277 ± 0.02
0.54TyrAsn: 0.54 ± 0.03
0.962TyrPro: 0.962 ± 0.037
0.786TyrGln: 0.786 ± 0.031
1.554TyrArg: 1.554 ± 0.055
1.292TyrSer: 1.292 ± 0.053
0.864TyrThr: 0.864 ± 0.052
1.206TyrVal: 1.206 ± 0.044
0.36TyrTrp: 0.36 ± 0.023
0.414TyrTyr: 0.414 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2955 proteins (801681 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski