Clostridium sp. C105KSO13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2977 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5NSD2|A0A0U5NSD2_9CLOT Daunorubicin/doxorubicin resistance ATP-binding protein DrrA OS=Clostridium sp. C105KSO13 OX=1776045 GN=drrA_1 PE=4 SV=1
MM1 pKa = 7.56LLALGEE7 pKa = 4.16GDD9 pKa = 3.99KK10 pKa = 10.95IAGRR14 pKa = 11.84EE15 pKa = 4.16TKK17 pKa = 10.38DD18 pKa = 3.16LNAAADD24 pKa = 3.82LTGTGWADD32 pKa = 2.84ISYY35 pKa = 9.33EE36 pKa = 3.8QLIAYY41 pKa = 8.77NPDD44 pKa = 3.69MIVLISDD51 pKa = 3.7ADD53 pKa = 3.8YY54 pKa = 11.36SVEE57 pKa = 4.62DD58 pKa = 3.99VLNDD62 pKa = 3.65GQLAGIKK69 pKa = 9.62AVQNGAVYY77 pKa = 10.04QMPKK81 pKa = 10.39GYY83 pKa = 9.35EE84 pKa = 3.87AWDD87 pKa = 3.81SPVPATILGSLWLAAVIVPDD107 pKa = 5.34AYY109 pKa = 11.31SQDD112 pKa = 4.03DD113 pKa = 3.85FVKK116 pKa = 10.48EE117 pKa = 3.72AEE119 pKa = 4.16TFYY122 pKa = 11.33KK123 pKa = 10.04EE124 pKa = 4.18FYY126 pKa = 10.63GIAIDD131 pKa = 4.05TTQLTQQ137 pKa = 3.1

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U5NU22|A0A0U5NU22_9CLOT Putative endopeptidase p60 OS=Clostridium sp. C105KSO13 OX=1776045 GN=iap_2 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2977

0

2977

938323

29

2714

315.2

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.373 ± 0.039

1.4 ± 0.017

5.541 ± 0.037

7.595 ± 0.053

3.957 ± 0.033

7.223 ± 0.045

1.759 ± 0.02

7.503 ± 0.042

7.148 ± 0.039

8.932 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.023

4.38 ± 0.029

3.353 ± 0.024

3.294 ± 0.025

4.329 ± 0.034

5.804 ± 0.034

5.478 ± 0.035

6.925 ± 0.039

0.865 ± 0.016

3.998 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski