Amino acid dipepetide frequency for Clostridium sp. C105KSO13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.303AlaAla: 7.303 ± 0.116
1.107AlaCys: 1.107 ± 0.037
4.441AlaAsp: 4.441 ± 0.078
5.341AlaGlu: 5.341 ± 0.084
2.869AlaPhe: 2.869 ± 0.052
6.314AlaGly: 6.314 ± 0.089
1.109AlaHis: 1.109 ± 0.032
5.088AlaIle: 5.088 ± 0.078
5.014AlaLys: 5.014 ± 0.072
6.865AlaLeu: 6.865 ± 0.094
2.256AlaMet: 2.256 ± 0.049
2.468AlaAsn: 2.468 ± 0.054
2.0AlaPro: 2.0 ± 0.054
2.312AlaGln: 2.312 ± 0.06
2.918AlaArg: 2.918 ± 0.055
3.767AlaSer: 3.767 ± 0.074
3.074AlaThr: 3.074 ± 0.073
6.274AlaVal: 6.274 ± 0.092
0.562AlaTrp: 0.562 ± 0.024
2.641AlaTyr: 2.641 ± 0.052
0.0AlaXaa: 0.0 ± 0.0
Cys
0.983CysAla: 0.983 ± 0.035
0.25CysCys: 0.25 ± 0.02
0.755CysAsp: 0.755 ± 0.028
0.924CysGlu: 0.924 ± 0.034
0.516CysPhe: 0.516 ± 0.023
1.417CysGly: 1.417 ± 0.046
0.295CysHis: 0.295 ± 0.02
1.115CysIle: 1.115 ± 0.039
0.783CysLys: 0.783 ± 0.033
1.028CysLeu: 1.028 ± 0.033
0.444CysMet: 0.444 ± 0.025
0.535CysAsn: 0.535 ± 0.023
0.627CysPro: 0.627 ± 0.027
0.38CysGln: 0.38 ± 0.022
0.75CysArg: 0.75 ± 0.029
0.891CysSer: 0.891 ± 0.034
0.76CysThr: 0.76 ± 0.031
0.94CysVal: 0.94 ± 0.033
0.109CysTrp: 0.109 ± 0.01
0.497CysTyr: 0.497 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.918AspAla: 3.918 ± 0.087
0.746AspCys: 0.746 ± 0.029
2.804AspAsp: 2.804 ± 0.076
4.556AspGlu: 4.556 ± 0.076
2.519AspPhe: 2.519 ± 0.056
4.004AspGly: 4.004 ± 0.074
0.781AspHis: 0.781 ± 0.032
4.793AspIle: 4.793 ± 0.072
3.929AspLys: 3.929 ± 0.072
4.421AspLeu: 4.421 ± 0.084
1.991AspMet: 1.991 ± 0.047
2.149AspAsn: 2.149 ± 0.047
1.62AspPro: 1.62 ± 0.037
1.214AspGln: 1.214 ± 0.041
2.288AspArg: 2.288 ± 0.054
3.189AspSer: 3.189 ± 0.065
3.327AspThr: 3.327 ± 0.06
3.963AspVal: 3.963 ± 0.068
0.556AspTrp: 0.556 ± 0.027
2.643AspTyr: 2.643 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.433GluAla: 5.433 ± 0.074
0.763GluCys: 0.763 ± 0.031
4.206GluAsp: 4.206 ± 0.075
7.275GluGlu: 7.275 ± 0.118
2.503GluPhe: 2.503 ± 0.053
4.628GluGly: 4.628 ± 0.078
1.341GluHis: 1.341 ± 0.042
5.643GluIle: 5.643 ± 0.09
6.896GluLys: 6.896 ± 0.094
6.821GluLeu: 6.821 ± 0.1
2.384GluMet: 2.384 ± 0.043
4.132GluAsn: 4.132 ± 0.073
1.995GluPro: 1.995 ± 0.048
2.996GluGln: 2.996 ± 0.063
3.211GluArg: 3.211 ± 0.072
3.507GluSer: 3.507 ± 0.057
3.752GluThr: 3.752 ± 0.07
4.724GluVal: 4.724 ± 0.071
0.602GluTrp: 0.602 ± 0.03
3.14GluTyr: 3.14 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
2.751PheAla: 2.751 ± 0.065
0.664PheCys: 0.664 ± 0.027
2.369PheAsp: 2.369 ± 0.06
2.671PheGlu: 2.671 ± 0.054
1.716PhePhe: 1.716 ± 0.05
2.905PheGly: 2.905 ± 0.056
0.767PheHis: 0.767 ± 0.027
2.88PheIle: 2.88 ± 0.052
2.162PheLys: 2.162 ± 0.048
3.833PheLeu: 3.833 ± 0.07
1.2PheMet: 1.2 ± 0.035
1.524PheAsn: 1.524 ± 0.039
1.422PhePro: 1.422 ± 0.04
1.353PheGln: 1.353 ± 0.036
1.681PheArg: 1.681 ± 0.049
2.756PheSer: 2.756 ± 0.055
2.319PheThr: 2.319 ± 0.051
2.604PheVal: 2.604 ± 0.057
0.395PheTrp: 0.395 ± 0.021
1.598PheTyr: 1.598 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
5.146GlyAla: 5.146 ± 0.091
1.231GlyCys: 1.231 ± 0.042
3.589GlyAsp: 3.589 ± 0.071
4.798GlyGlu: 4.798 ± 0.082
3.087GlyPhe: 3.087 ± 0.06
5.052GlyGly: 5.052 ± 0.089
1.303GlyHis: 1.303 ± 0.04
6.304GlyIle: 6.304 ± 0.091
5.775GlyLys: 5.775 ± 0.074
5.93GlyLeu: 5.93 ± 0.094
2.569GlyMet: 2.569 ± 0.055
3.306GlyAsn: 3.306 ± 0.069
1.437GlyPro: 1.437 ± 0.052
2.106GlyGln: 2.106 ± 0.053
3.167GlyArg: 3.167 ± 0.066
4.07GlySer: 4.07 ± 0.071
4.475GlyThr: 4.475 ± 0.077
5.081GlyVal: 5.081 ± 0.08
0.629GlyTrp: 0.629 ± 0.029
3.171GlyTyr: 3.171 ± 0.054
0.0GlyXaa: 0.0 ± 0.0
His
1.106HisAla: 1.106 ± 0.035
0.308HisCys: 0.308 ± 0.02
0.849HisAsp: 0.849 ± 0.031
1.1HisGlu: 1.1 ± 0.033
0.848HisPhe: 0.848 ± 0.029
1.277HisGly: 1.277 ± 0.04
0.422HisHis: 0.422 ± 0.024
1.486HisIle: 1.486 ± 0.038
1.0HisLys: 1.0 ± 0.033
1.605HisLeu: 1.605 ± 0.048
0.57HisMet: 0.57 ± 0.025
0.747HisAsn: 0.747 ± 0.028
0.902HisPro: 0.902 ± 0.029
0.575HisGln: 0.575 ± 0.024
0.79HisArg: 0.79 ± 0.027
0.958HisSer: 0.958 ± 0.033
1.01HisThr: 1.01 ± 0.033
1.146HisVal: 1.146 ± 0.036
0.161HisTrp: 0.161 ± 0.014
0.733HisTyr: 0.733 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.76IleAla: 5.76 ± 0.089
1.231IleCys: 1.231 ± 0.038
3.959IleAsp: 3.959 ± 0.075
4.891IleGlu: 4.891 ± 0.077
3.023IlePhe: 3.023 ± 0.062
5.21IleGly: 5.21 ± 0.086
1.456IleHis: 1.456 ± 0.044
5.42IleIle: 5.42 ± 0.098
4.42IleLys: 4.42 ± 0.071
7.339IleLeu: 7.339 ± 0.109
2.186IleMet: 2.186 ± 0.056
2.983IleAsn: 2.983 ± 0.067
3.24IlePro: 3.24 ± 0.065
2.532IleGln: 2.532 ± 0.052
3.537IleArg: 3.537 ± 0.065
4.998IleSer: 4.998 ± 0.079
4.436IleThr: 4.436 ± 0.081
5.087IleVal: 5.087 ± 0.074
0.622IleTrp: 0.622 ± 0.027
2.705IleTyr: 2.705 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.232LysAla: 5.232 ± 0.077
0.71LysCys: 0.71 ± 0.03
4.16LysAsp: 4.16 ± 0.073
7.107LysGlu: 7.107 ± 0.092
2.017LysPhe: 2.017 ± 0.05
4.536LysGly: 4.536 ± 0.072
1.082LysHis: 1.082 ± 0.035
4.981LysIle: 4.981 ± 0.066
6.297LysLys: 6.297 ± 0.088
5.593LysLeu: 5.593 ± 0.073
2.326LysMet: 2.326 ± 0.053
3.602LysAsn: 3.602 ± 0.061
2.089LysPro: 2.089 ± 0.05
2.435LysGln: 2.435 ± 0.059
3.242LysArg: 3.242 ± 0.064
3.729LysSer: 3.729 ± 0.073
3.971LysThr: 3.971 ± 0.064
4.866LysVal: 4.866 ± 0.079
0.639LysTrp: 0.639 ± 0.029
2.869LysTyr: 2.869 ± 0.064
0.0LysXaa: 0.0 ± 0.0
Leu
6.604LeuAla: 6.604 ± 0.097
1.393LeuCys: 1.393 ± 0.044
4.979LeuAsp: 4.979 ± 0.076
6.601LeuGlu: 6.601 ± 0.104
3.562LeuPhe: 3.562 ± 0.075
6.269LeuGly: 6.269 ± 0.103
1.59LeuHis: 1.59 ± 0.042
6.093LeuIle: 6.093 ± 0.087
6.63LeuLys: 6.63 ± 0.088
8.42LeuLeu: 8.42 ± 0.122
2.535LeuMet: 2.535 ± 0.05
4.032LeuAsn: 4.032 ± 0.069
3.459LeuPro: 3.459 ± 0.074
2.95LeuGln: 2.95 ± 0.06
3.599LeuArg: 3.599 ± 0.067
6.173LeuSer: 6.173 ± 0.092
5.074LeuThr: 5.074 ± 0.079
5.43LeuVal: 5.43 ± 0.081
0.743LeuTrp: 0.743 ± 0.031
3.183LeuTyr: 3.183 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.39MetAla: 2.39 ± 0.052
0.378MetCys: 0.378 ± 0.021
1.927MetAsp: 1.927 ± 0.042
2.553MetGlu: 2.553 ± 0.057
1.012MetPhe: 1.012 ± 0.035
2.218MetGly: 2.218 ± 0.05
0.521MetHis: 0.521 ± 0.023
2.187MetIle: 2.187 ± 0.054
2.628MetLys: 2.628 ± 0.053
2.846MetLeu: 2.846 ± 0.059
0.918MetMet: 0.918 ± 0.034
1.656MetAsn: 1.656 ± 0.045
1.099MetPro: 1.099 ± 0.033
1.104MetGln: 1.104 ± 0.042
1.277MetArg: 1.277 ± 0.037
1.869MetSer: 1.869 ± 0.048
1.773MetThr: 1.773 ± 0.049
1.897MetVal: 1.897 ± 0.04
0.208MetTrp: 0.208 ± 0.015
0.957MetTyr: 0.957 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.051AsnAla: 3.051 ± 0.064
0.599AsnCys: 0.599 ± 0.026
2.018AsnAsp: 2.018 ± 0.06
2.825AsnGlu: 2.825 ± 0.054
1.669AsnPhe: 1.669 ± 0.048
3.213AsnGly: 3.213 ± 0.066
0.849AsnHis: 0.849 ± 0.031
3.604AsnIle: 3.604 ± 0.063
2.692AsnLys: 2.692 ± 0.052
3.93AsnLeu: 3.93 ± 0.073
1.497AsnMet: 1.497 ± 0.043
1.877AsnAsn: 1.877 ± 0.044
2.127AsnPro: 2.127 ± 0.051
1.592AsnGln: 1.592 ± 0.048
2.062AsnArg: 2.062 ± 0.048
2.38AsnSer: 2.38 ± 0.049
2.526AsnThr: 2.526 ± 0.055
2.985AsnVal: 2.985 ± 0.062
0.425AsnTrp: 0.425 ± 0.02
1.877AsnTyr: 1.877 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
2.441ProAla: 2.441 ± 0.049
0.451ProCys: 0.451 ± 0.023
2.337ProAsp: 2.337 ± 0.057
3.126ProGlu: 3.126 ± 0.057
1.518ProPhe: 1.518 ± 0.042
2.547ProGly: 2.547 ± 0.057
0.629ProHis: 0.629 ± 0.025
2.198ProIle: 2.198 ± 0.051
2.074ProLys: 2.074 ± 0.047
2.768ProLeu: 2.768 ± 0.054
0.902ProMet: 0.902 ± 0.035
1.269ProAsn: 1.269 ± 0.046
0.824ProPro: 0.824 ± 0.035
1.034ProGln: 1.034 ± 0.038
1.129ProArg: 1.129 ± 0.037
1.892ProSer: 1.892 ± 0.051
1.632ProThr: 1.632 ± 0.04
2.96ProVal: 2.96 ± 0.059
0.308ProTrp: 0.308 ± 0.019
1.494ProTyr: 1.494 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.471GlnAla: 2.471 ± 0.063
0.361GlnCys: 0.361 ± 0.019
1.66GlnAsp: 1.66 ± 0.046
2.665GlnGlu: 2.665 ± 0.062
1.158GlnPhe: 1.158 ± 0.032
2.069GlnGly: 2.069 ± 0.048
0.51GlnHis: 0.51 ± 0.025
2.594GlnIle: 2.594 ± 0.057
2.876GlnLys: 2.876 ± 0.058
2.838GlnLeu: 2.838 ± 0.058
1.232GlnMet: 1.232 ± 0.035
1.688GlnAsn: 1.688 ± 0.043
0.945GlnPro: 0.945 ± 0.039
1.173GlnGln: 1.173 ± 0.036
1.417GlnArg: 1.417 ± 0.041
1.685GlnSer: 1.685 ± 0.042
1.76GlnThr: 1.76 ± 0.042
2.187GlnVal: 2.187 ± 0.05
0.28GlnTrp: 0.28 ± 0.019
1.366GlnTyr: 1.366 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
2.687ArgAla: 2.687 ± 0.05
0.546ArgCys: 0.546 ± 0.029
2.206ArgAsp: 2.206 ± 0.064
3.702ArgGlu: 3.702 ± 0.078
1.783ArgPhe: 1.783 ± 0.043
2.675ArgGly: 2.675 ± 0.055
0.84ArgHis: 0.84 ± 0.031
3.417ArgIle: 3.417 ± 0.065
3.569ArgLys: 3.569 ± 0.07
3.81ArgLeu: 3.81 ± 0.07
1.486ArgMet: 1.486 ± 0.039
2.008ArgAsn: 2.008 ± 0.052
1.319ArgPro: 1.319 ± 0.038
1.802ArgGln: 1.802 ± 0.05
2.255ArgArg: 2.255 ± 0.05
2.098ArgSer: 2.098 ± 0.051
2.239ArgThr: 2.239 ± 0.055
2.674ArgVal: 2.674 ± 0.048
0.356ArgTrp: 0.356 ± 0.019
1.817ArgTyr: 1.817 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
4.162SerAla: 4.162 ± 0.063
0.806SerCys: 0.806 ± 0.031
3.264SerAsp: 3.264 ± 0.069
3.842SerGlu: 3.842 ± 0.069
2.515SerPhe: 2.515 ± 0.051
5.076SerGly: 5.076 ± 0.092
1.027SerHis: 1.027 ± 0.037
4.242SerIle: 4.242 ± 0.059
3.497SerLys: 3.497 ± 0.067
5.117SerLeu: 5.117 ± 0.083
1.898SerMet: 1.898 ± 0.044
2.336SerAsn: 2.336 ± 0.053
1.847SerPro: 1.847 ± 0.052
1.888SerGln: 1.888 ± 0.045
2.731SerArg: 2.731 ± 0.061
3.588SerSer: 3.588 ± 0.079
2.978SerThr: 2.978 ± 0.054
4.219SerVal: 4.219 ± 0.071
0.57SerTrp: 0.57 ± 0.027
2.437SerTyr: 2.437 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
4.54ThrAla: 4.54 ± 0.083
0.615ThrCys: 0.615 ± 0.029
3.177ThrAsp: 3.177 ± 0.067
3.768ThrGlu: 3.768 ± 0.063
2.242ThrPhe: 2.242 ± 0.054
4.981ThrGly: 4.981 ± 0.068
0.86ThrHis: 0.86 ± 0.031
3.976ThrIle: 3.976 ± 0.077
3.373ThrLys: 3.373 ± 0.06
4.932ThrLeu: 4.932 ± 0.08
1.535ThrMet: 1.535 ± 0.042
2.158ThrAsn: 2.158 ± 0.047
2.264ThrPro: 2.264 ± 0.057
1.547ThrGln: 1.547 ± 0.046
2.016ThrArg: 2.016 ± 0.053
2.968ThrSer: 2.968 ± 0.054
2.858ThrThr: 2.858 ± 0.061
4.383ThrVal: 4.383 ± 0.082
0.468ThrTrp: 0.468 ± 0.021
2.117ThrTyr: 2.117 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
4.549ValAla: 4.549 ± 0.075
1.102ValCys: 1.102 ± 0.032
3.83ValAsp: 3.83 ± 0.066
4.6ValGlu: 4.6 ± 0.076
2.875ValPhe: 2.875 ± 0.055
4.363ValGly: 4.363 ± 0.075
1.215ValHis: 1.215 ± 0.034
5.535ValIle: 5.535 ± 0.08
4.751ValLys: 4.751 ± 0.075
6.859ValLeu: 6.859 ± 0.1
2.057ValMet: 2.057 ± 0.051
2.978ValAsn: 2.978 ± 0.056
2.624ValPro: 2.624 ± 0.054
2.145ValGln: 2.145 ± 0.048
2.973ValArg: 2.973 ± 0.058
4.747ValSer: 4.747 ± 0.084
4.08ValThr: 4.08 ± 0.081
4.744ValVal: 4.744 ± 0.09
0.579ValTrp: 0.579 ± 0.028
2.643ValTyr: 2.643 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.552TrpAla: 0.552 ± 0.032
0.135TrpCys: 0.135 ± 0.012
0.553TrpAsp: 0.553 ± 0.024
0.648TrpGlu: 0.648 ± 0.029
0.385TrpPhe: 0.385 ± 0.021
0.62TrpGly: 0.62 ± 0.03
0.153TrpHis: 0.153 ± 0.012
0.593TrpIle: 0.593 ± 0.026
0.655TrpLys: 0.655 ± 0.029
0.802TrpLeu: 0.802 ± 0.031
0.283TrpMet: 0.283 ± 0.019
0.54TrpAsn: 0.54 ± 0.032
0.22TrpPro: 0.22 ± 0.016
0.339TrpGln: 0.339 ± 0.022
0.341TrpArg: 0.341 ± 0.019
0.458TrpSer: 0.458 ± 0.023
0.418TrpThr: 0.418 ± 0.022
0.52TrpVal: 0.52 ± 0.022
0.087TrpTrp: 0.087 ± 0.011
0.351TrpTyr: 0.351 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.057
0.603TyrCys: 0.603 ± 0.027
2.325TyrAsp: 2.325 ± 0.053
2.96TyrGlu: 2.96 ± 0.058
1.75TyrPhe: 1.75 ± 0.053
2.852TyrGly: 2.852 ± 0.058
0.841TyrHis: 0.841 ± 0.03
2.888TyrIle: 2.888 ± 0.065
2.461TyrLys: 2.461 ± 0.055
3.562TyrLeu: 3.562 ± 0.07
1.148TyrMet: 1.148 ± 0.039
1.813TyrAsn: 1.813 ± 0.046
1.471TyrPro: 1.471 ± 0.04
1.46TyrGln: 1.46 ± 0.046
1.903TyrArg: 1.903 ± 0.047
2.317TyrSer: 2.317 ± 0.051
2.319TyrThr: 2.319 ± 0.057
2.564TyrVal: 2.564 ± 0.055
0.355TyrTrp: 0.355 ± 0.021
1.747TyrTyr: 1.747 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2977 proteins (938323 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski