Streptomyces phage Keanu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hiyaavirus; unclassified Hiyaavirus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LKD9|A0A7G8LKD9_9CAUD Uncharacterized protein OS=Streptomyces phage Keanu OX=2762406 GN=106 PE=4 SV=1
MM1 pKa = 7.53TEE3 pKa = 3.75QPKK6 pKa = 10.77VKK8 pKa = 10.2VALEE12 pKa = 4.12LVVEE16 pKa = 4.66IDD18 pKa = 3.46PEE20 pKa = 4.15AWEE23 pKa = 4.37HH24 pKa = 4.49VHH26 pKa = 6.93NGEE29 pKa = 4.04WKK31 pKa = 10.48LNQATALAYY40 pKa = 9.93VMQMFEE46 pKa = 4.68ADD48 pKa = 3.38AKK50 pKa = 10.09GTGITQVVHH59 pKa = 6.83LEE61 pKa = 4.01ATDD64 pKa = 3.95PEE66 pKa = 4.44TGEE69 pKa = 4.67NVDD72 pKa = 4.68EE73 pKa = 6.38DD74 pKa = 4.77GTCPDD79 pKa = 5.09CGDD82 pKa = 4.66SMDD85 pKa = 5.16EE86 pKa = 4.19DD87 pKa = 5.13DD88 pKa = 5.87EE89 pKa = 6.05GNTSCPNGCEE99 pKa = 4.04NVV101 pKa = 3.31

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LK72|A0A7G8LK72_9CAUD Uncharacterized protein OS=Streptomyces phage Keanu OX=2762406 GN=38 PE=4 SV=1
MM1 pKa = 7.7NDD3 pKa = 3.44RR4 pKa = 11.84EE5 pKa = 4.14QVAWGRR11 pKa = 11.84LSEE14 pKa = 4.09VAPRR18 pKa = 11.84GRR20 pKa = 11.84YY21 pKa = 5.89EE22 pKa = 3.98HH23 pKa = 6.95PRR25 pKa = 11.84TRR27 pKa = 11.84TKK29 pKa = 10.42RR30 pKa = 11.84VKK32 pKa = 9.67QRR34 pKa = 11.84QNFVSRR40 pKa = 11.84IDD42 pKa = 3.45RR43 pKa = 11.84RR44 pKa = 11.84DD45 pKa = 3.35GHH47 pKa = 6.4WIWTGQTTTRR57 pKa = 11.84DD58 pKa = 3.36GKK60 pKa = 9.95RR61 pKa = 11.84YY62 pKa = 9.71PMFSYY67 pKa = 10.65RR68 pKa = 11.84ILGGGRR74 pKa = 11.84DD75 pKa = 3.44SRR77 pKa = 11.84RR78 pKa = 11.84QVMAFRR84 pKa = 11.84HH85 pKa = 5.4LMSEE89 pKa = 4.59CFPEE93 pKa = 4.65LVAKK97 pKa = 10.42IPQRR101 pKa = 11.84TAPACGEE108 pKa = 4.09PMCVYY113 pKa = 9.55PWHH116 pKa = 7.2RR117 pKa = 11.84ANMMNTRR124 pKa = 11.84QTITGEE130 pKa = 3.83QALAIYY136 pKa = 8.04QAKK139 pKa = 7.98GTRR142 pKa = 11.84SAADD146 pKa = 3.44LASEE150 pKa = 4.29YY151 pKa = 10.61GISSEE156 pKa = 4.07QVNAIWRR163 pKa = 11.84GRR165 pKa = 11.84SWRR168 pKa = 11.84SVTGAPVPIPKK179 pKa = 10.06RR180 pKa = 11.84KK181 pKa = 10.26VYY183 pKa = 10.74DD184 pKa = 3.62DD185 pKa = 4.01DD186 pKa = 3.56MVRR189 pKa = 11.84RR190 pKa = 11.84VLDD193 pKa = 3.78LKK195 pKa = 10.67GTGSSRR201 pKa = 11.84AVAAEE206 pKa = 3.77LGIGYY211 pKa = 9.86KK212 pKa = 10.15FVLDD216 pKa = 3.07VWAGVRR222 pKa = 11.84QPKK225 pKa = 10.33GSLSVAQDD233 pKa = 3.04AA234 pKa = 4.56

Molecular weight: 26.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

114

0

114

24315

29

1106

213.3

23.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.014 ± 0.398

1.003 ± 0.114

6.514 ± 0.252

6.062 ± 0.301

2.702 ± 0.137

8.702 ± 0.262

1.995 ± 0.116

4.125 ± 0.171

4.351 ± 0.22

7.267 ± 0.213

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.295 ± 0.104

3.438 ± 0.155

5.359 ± 0.263

3.759 ± 0.163

6.967 ± 0.313

5.47 ± 0.238

6.876 ± 0.275

7.514 ± 0.204

2.126 ± 0.109

2.459 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski