Amino acid dipepetide frequency for Streptomyces phage Keanu

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.531AlaAla: 13.531 ± 1.079
1.11AlaCys: 1.11 ± 0.207
7.444AlaAsp: 7.444 ± 0.575
6.704AlaGlu: 6.704 ± 0.703
2.509AlaPhe: 2.509 ± 0.346
8.472AlaGly: 8.472 ± 0.675
1.563AlaHis: 1.563 ± 0.298
4.401AlaIle: 4.401 ± 0.53
6.581AlaLys: 6.581 ± 0.763
9.254AlaLeu: 9.254 ± 0.651
2.838AlaMet: 2.838 ± 0.322
3.578AlaAsn: 3.578 ± 0.448
5.47AlaPro: 5.47 ± 0.69
4.771AlaGln: 4.771 ± 0.368
6.539AlaArg: 6.539 ± 0.543
6.087AlaSer: 6.087 ± 0.585
7.239AlaThr: 7.239 ± 0.629
7.568AlaVal: 7.568 ± 0.683
2.385AlaTrp: 2.385 ± 0.297
2.098AlaTyr: 2.098 ± 0.292
0.0AlaXaa: 0.0 ± 0.0
Cys
0.987CysAla: 0.987 ± 0.211
0.247CysCys: 0.247 ± 0.118
0.699CysAsp: 0.699 ± 0.184
0.37CysGlu: 0.37 ± 0.124
0.37CysPhe: 0.37 ± 0.126
1.357CysGly: 1.357 ± 0.299
0.247CysHis: 0.247 ± 0.103
0.247CysIle: 0.247 ± 0.111
0.411CysLys: 0.411 ± 0.104
0.452CysLeu: 0.452 ± 0.126
0.082CysMet: 0.082 ± 0.058
0.288CysAsn: 0.288 ± 0.113
0.905CysPro: 0.905 ± 0.312
0.494CysGln: 0.494 ± 0.134
0.864CysArg: 0.864 ± 0.178
0.699CysSer: 0.699 ± 0.152
0.617CysThr: 0.617 ± 0.139
0.329CysVal: 0.329 ± 0.118
0.165CysTrp: 0.165 ± 0.085
0.206CysTyr: 0.206 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
7.979AspAla: 7.979 ± 0.514
0.699AspCys: 0.699 ± 0.198
5.223AspAsp: 5.223 ± 0.432
4.771AspGlu: 4.771 ± 0.554
2.18AspPhe: 2.18 ± 0.255
5.223AspGly: 5.223 ± 0.351
1.275AspHis: 1.275 ± 0.24
2.55AspIle: 2.55 ± 0.358
1.727AspLys: 1.727 ± 0.261
6.046AspLeu: 6.046 ± 0.475
1.316AspMet: 1.316 ± 0.24
2.015AspAsn: 2.015 ± 0.28
4.195AspPro: 4.195 ± 0.395
2.509AspGln: 2.509 ± 0.329
3.784AspArg: 3.784 ± 0.406
3.002AspSer: 3.002 ± 0.423
3.743AspThr: 3.743 ± 0.506
4.318AspVal: 4.318 ± 0.408
1.11AspTrp: 1.11 ± 0.217
1.481AspTyr: 1.481 ± 0.28
0.0AspXaa: 0.0 ± 0.0
Glu
7.444GluAla: 7.444 ± 0.612
0.74GluCys: 0.74 ± 0.159
3.989GluAsp: 3.989 ± 0.433
3.702GluGlu: 3.702 ± 0.441
2.098GluPhe: 2.098 ± 0.275
5.306GluGly: 5.306 ± 0.534
1.193GluHis: 1.193 ± 0.228
2.879GluIle: 2.879 ± 0.321
1.481GluLys: 1.481 ± 0.25
5.018GluLeu: 5.018 ± 0.388
1.152GluMet: 1.152 ± 0.186
1.851GluAsn: 1.851 ± 0.268
2.756GluPro: 2.756 ± 0.424
2.55GluGln: 2.55 ± 0.369
4.442GluArg: 4.442 ± 0.544
2.385GluSer: 2.385 ± 0.287
3.249GluThr: 3.249 ± 0.34
5.141GluVal: 5.141 ± 0.461
1.522GluTrp: 1.522 ± 0.243
1.686GluTyr: 1.686 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.838PheAla: 2.838 ± 0.332
0.247PheCys: 0.247 ± 0.11
2.098PheAsp: 2.098 ± 0.247
1.727PheGlu: 1.727 ± 0.222
0.576PhePhe: 0.576 ± 0.151
2.591PheGly: 2.591 ± 0.376
0.329PheHis: 0.329 ± 0.112
1.028PheIle: 1.028 ± 0.172
1.193PheLys: 1.193 ± 0.236
1.727PheLeu: 1.727 ± 0.254
0.535PheMet: 0.535 ± 0.143
1.193PheAsn: 1.193 ± 0.254
1.069PhePro: 1.069 ± 0.225
0.864PheGln: 0.864 ± 0.166
2.015PheArg: 2.015 ± 0.283
1.522PheSer: 1.522 ± 0.291
1.645PheThr: 1.645 ± 0.289
2.098PheVal: 2.098 ± 0.338
0.617PheTrp: 0.617 ± 0.176
1.11PheTyr: 1.11 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
7.773GlyAla: 7.773 ± 0.719
1.11GlyCys: 1.11 ± 0.237
5.552GlyAsp: 5.552 ± 0.573
4.113GlyGlu: 4.113 ± 0.462
3.044GlyPhe: 3.044 ± 0.318
7.568GlyGly: 7.568 ± 0.831
2.797GlyHis: 2.797 ± 0.424
3.414GlyIle: 3.414 ± 0.456
3.989GlyLys: 3.989 ± 0.381
5.964GlyLeu: 5.964 ± 0.534
1.851GlyMet: 1.851 ± 0.268
3.249GlyAsn: 3.249 ± 0.298
3.126GlyPro: 3.126 ± 0.345
3.414GlyGln: 3.414 ± 0.372
7.033GlyArg: 7.033 ± 0.577
5.635GlySer: 5.635 ± 0.816
6.663GlyThr: 6.663 ± 0.754
6.581GlyVal: 6.581 ± 0.48
2.056GlyTrp: 2.056 ± 0.274
2.098GlyTyr: 2.098 ± 0.314
0.0GlyXaa: 0.0 ± 0.0
His
2.221HisAla: 2.221 ± 0.331
0.123HisCys: 0.123 ± 0.073
1.234HisAsp: 1.234 ± 0.209
1.727HisGlu: 1.727 ± 0.245
0.411HisPhe: 0.411 ± 0.124
1.769HisGly: 1.769 ± 0.273
0.329HisHis: 0.329 ± 0.126
0.864HisIle: 0.864 ± 0.182
0.699HisLys: 0.699 ± 0.185
1.769HisLeu: 1.769 ± 0.335
0.452HisMet: 0.452 ± 0.134
0.535HisAsn: 0.535 ± 0.129
1.522HisPro: 1.522 ± 0.285
0.905HisGln: 0.905 ± 0.225
0.987HisArg: 0.987 ± 0.209
1.193HisSer: 1.193 ± 0.208
0.864HisThr: 0.864 ± 0.178
1.357HisVal: 1.357 ± 0.225
0.329HisTrp: 0.329 ± 0.129
0.658HisTyr: 0.658 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
3.619IleAla: 3.619 ± 0.477
0.452IleCys: 0.452 ± 0.147
3.044IleAsp: 3.044 ± 0.369
3.331IleGlu: 3.331 ± 0.445
0.658IlePhe: 0.658 ± 0.166
3.373IleGly: 3.373 ± 0.324
0.494IleHis: 0.494 ± 0.148
1.933IleIle: 1.933 ± 0.314
2.55IleLys: 2.55 ± 0.556
2.18IleLeu: 2.18 ± 0.283
0.699IleMet: 0.699 ± 0.162
1.892IleAsn: 1.892 ± 0.291
2.344IlePro: 2.344 ± 0.314
1.193IleGln: 1.193 ± 0.253
3.249IleArg: 3.249 ± 0.338
2.344IleSer: 2.344 ± 0.383
3.496IleThr: 3.496 ± 0.47
2.673IleVal: 2.673 ± 0.384
0.781IleTrp: 0.781 ± 0.177
0.946IleTyr: 0.946 ± 0.169
0.0IleXaa: 0.0 ± 0.0
Lys
5.799LysAla: 5.799 ± 0.702
0.206LysCys: 0.206 ± 0.086
2.056LysAsp: 2.056 ± 0.301
1.851LysGlu: 1.851 ± 0.324
1.234LysPhe: 1.234 ± 0.22
3.496LysGly: 3.496 ± 0.501
1.028LysHis: 1.028 ± 0.202
1.892LysIle: 1.892 ± 0.293
2.056LysLys: 2.056 ± 0.307
3.167LysLeu: 3.167 ± 0.397
0.781LysMet: 0.781 ± 0.182
1.028LysAsn: 1.028 ± 0.211
2.303LysPro: 2.303 ± 0.3
1.974LysGln: 1.974 ± 0.358
3.414LysArg: 3.414 ± 0.409
2.632LysSer: 2.632 ± 0.478
2.468LysThr: 2.468 ± 0.311
4.113LysVal: 4.113 ± 0.441
1.275LysTrp: 1.275 ± 0.206
0.74LysTyr: 0.74 ± 0.211
0.0LysXaa: 0.0 ± 0.0
Leu
9.048LeuAla: 9.048 ± 0.681
0.535LeuCys: 0.535 ± 0.139
5.47LeuAsp: 5.47 ± 0.442
3.619LeuGlu: 3.619 ± 0.429
1.81LeuPhe: 1.81 ± 0.268
6.128LeuGly: 6.128 ± 0.54
1.522LeuHis: 1.522 ± 0.24
3.578LeuIle: 3.578 ± 0.35
3.29LeuLys: 3.29 ± 0.368
5.306LeuLeu: 5.306 ± 0.561
2.303LeuMet: 2.303 ± 0.284
2.098LeuAsn: 2.098 ± 0.262
3.866LeuPro: 3.866 ± 0.382
1.892LeuGln: 1.892 ± 0.258
5.059LeuArg: 5.059 ± 0.414
3.578LeuSer: 3.578 ± 0.364
5.1LeuThr: 5.1 ± 0.454
6.005LeuVal: 6.005 ± 0.469
1.028LeuTrp: 1.028 ± 0.218
1.439LeuTyr: 1.439 ± 0.225
0.0LeuXaa: 0.0 ± 0.0
Met
2.385MetAla: 2.385 ± 0.286
0.288MetCys: 0.288 ± 0.09
1.604MetAsp: 1.604 ± 0.267
1.11MetGlu: 1.11 ± 0.257
0.494MetPhe: 0.494 ± 0.132
1.851MetGly: 1.851 ± 0.34
0.535MetHis: 0.535 ± 0.137
0.864MetIle: 0.864 ± 0.198
0.699MetLys: 0.699 ± 0.154
1.316MetLeu: 1.316 ± 0.225
0.576MetMet: 0.576 ± 0.145
0.864MetAsn: 0.864 ± 0.186
1.398MetPro: 1.398 ± 0.276
0.658MetGln: 0.658 ± 0.176
2.139MetArg: 2.139 ± 0.259
1.686MetSer: 1.686 ± 0.273
1.686MetThr: 1.686 ± 0.24
1.604MetVal: 1.604 ± 0.237
0.494MetTrp: 0.494 ± 0.148
0.699MetTyr: 0.699 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.784AsnAla: 3.784 ± 0.558
0.123AsnCys: 0.123 ± 0.069
1.851AsnAsp: 1.851 ± 0.339
1.481AsnGlu: 1.481 ± 0.212
0.658AsnPhe: 0.658 ± 0.179
3.784AsnGly: 3.784 ± 0.429
0.535AsnHis: 0.535 ± 0.164
1.481AsnIle: 1.481 ± 0.23
0.781AsnLys: 0.781 ± 0.211
3.167AsnLeu: 3.167 ± 0.311
0.535AsnMet: 0.535 ± 0.135
1.357AsnAsn: 1.357 ± 0.233
2.303AsnPro: 2.303 ± 0.328
1.316AsnGln: 1.316 ± 0.221
2.632AsnArg: 2.632 ± 0.384
2.262AsnSer: 2.262 ± 0.343
2.262AsnThr: 2.262 ± 0.257
2.714AsnVal: 2.714 ± 0.366
0.658AsnTrp: 0.658 ± 0.141
0.699AsnTyr: 0.699 ± 0.163
0.0AsnXaa: 0.0 ± 0.0
Pro
5.593ProAla: 5.593 ± 0.638
0.494ProCys: 0.494 ± 0.133
3.496ProAsp: 3.496 ± 0.418
4.195ProGlu: 4.195 ± 0.495
1.152ProPhe: 1.152 ± 0.203
5.717ProGly: 5.717 ± 0.539
0.905ProHis: 0.905 ± 0.206
1.769ProIle: 1.769 ± 0.26
2.632ProLys: 2.632 ± 0.36
3.619ProLeu: 3.619 ± 0.425
1.316ProMet: 1.316 ± 0.231
2.262ProAsn: 2.262 ± 0.305
3.126ProPro: 3.126 ± 0.4
1.892ProGln: 1.892 ± 0.384
2.714ProArg: 2.714 ± 0.43
2.961ProSer: 2.961 ± 0.397
3.907ProThr: 3.907 ± 0.492
4.031ProVal: 4.031 ± 0.437
0.905ProTrp: 0.905 ± 0.184
0.905ProTyr: 0.905 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
3.907GlnAla: 3.907 ± 0.373
0.452GlnCys: 0.452 ± 0.139
2.18GlnAsp: 2.18 ± 0.305
2.139GlnGlu: 2.139 ± 0.31
1.028GlnPhe: 1.028 ± 0.199
3.578GlnGly: 3.578 ± 0.395
1.028GlnHis: 1.028 ± 0.292
1.81GlnIle: 1.81 ± 0.32
0.823GlnLys: 0.823 ± 0.165
2.961GlnLeu: 2.961 ± 0.361
1.234GlnMet: 1.234 ± 0.236
1.316GlnAsn: 1.316 ± 0.219
2.18GlnPro: 2.18 ± 0.38
1.563GlnGln: 1.563 ± 0.334
2.509GlnArg: 2.509 ± 0.329
1.686GlnSer: 1.686 ± 0.29
2.221GlnThr: 2.221 ± 0.327
3.496GlnVal: 3.496 ± 0.399
0.699GlnTrp: 0.699 ± 0.15
0.781GlnTyr: 0.781 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
6.91ArgAla: 6.91 ± 0.562
0.74ArgCys: 0.74 ± 0.245
4.154ArgAsp: 4.154 ± 0.471
4.401ArgGlu: 4.401 ± 0.511
1.974ArgPhe: 1.974 ± 0.252
3.989ArgGly: 3.989 ± 0.518
1.727ArgHis: 1.727 ± 0.279
2.879ArgIle: 2.879 ± 0.375
3.537ArgLys: 3.537 ± 0.408
5.059ArgLeu: 5.059 ± 0.453
2.098ArgMet: 2.098 ± 0.28
2.344ArgAsn: 2.344 ± 0.374
3.085ArgPro: 3.085 ± 0.333
2.92ArgGln: 2.92 ± 0.427
5.799ArgArg: 5.799 ± 0.654
3.66ArgSer: 3.66 ± 0.43
4.977ArgThr: 4.977 ± 0.5
5.347ArgVal: 5.347 ± 0.5
2.098ArgTrp: 2.098 ± 0.356
1.974ArgTyr: 1.974 ± 0.296
0.0ArgXaa: 0.0 ± 0.0
Ser
6.087SerAla: 6.087 ± 0.622
0.37SerCys: 0.37 ± 0.127
3.085SerAsp: 3.085 ± 0.332
3.126SerGlu: 3.126 ± 0.426
1.193SerPhe: 1.193 ± 0.253
6.622SerGly: 6.622 ± 0.791
1.234SerHis: 1.234 ± 0.212
2.18SerIle: 2.18 ± 0.319
2.673SerLys: 2.673 ± 0.334
3.126SerLeu: 3.126 ± 0.298
1.439SerMet: 1.439 ± 0.23
2.056SerAsn: 2.056 ± 0.294
2.468SerPro: 2.468 ± 0.338
1.645SerGln: 1.645 ± 0.239
3.331SerArg: 3.331 ± 0.548
3.455SerSer: 3.455 ± 0.447
4.154SerThr: 4.154 ± 0.525
3.784SerVal: 3.784 ± 0.392
0.905SerTrp: 0.905 ± 0.193
1.769SerTyr: 1.769 ± 0.285
0.0SerXaa: 0.0 ± 0.0
Thr
8.349ThrAla: 8.349 ± 0.803
0.781ThrCys: 0.781 ± 0.175
3.989ThrAsp: 3.989 ± 0.418
5.141ThrGlu: 5.141 ± 0.433
2.221ThrPhe: 2.221 ± 0.294
6.252ThrGly: 6.252 ± 0.554
0.781ThrHis: 0.781 ± 0.154
2.221ThrIle: 2.221 ± 0.276
2.961ThrLys: 2.961 ± 0.5
4.565ThrLeu: 4.565 ± 0.392
1.069ThrMet: 1.069 ± 0.195
2.262ThrAsn: 2.262 ± 0.313
5.758ThrPro: 5.758 ± 0.613
2.18ThrGln: 2.18 ± 0.304
3.866ThrArg: 3.866 ± 0.433
3.455ThrSer: 3.455 ± 0.447
5.47ThrThr: 5.47 ± 0.722
4.401ThrVal: 4.401 ± 0.464
1.439ThrTrp: 1.439 ± 0.249
1.604ThrTyr: 1.604 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
7.732ValAla: 7.732 ± 0.654
0.781ValCys: 0.781 ± 0.212
4.442ValAsp: 4.442 ± 0.446
4.195ValGlu: 4.195 ± 0.455
2.221ValPhe: 2.221 ± 0.276
6.334ValGly: 6.334 ± 0.591
1.481ValHis: 1.481 ± 0.292
3.455ValIle: 3.455 ± 0.379
3.578ValLys: 3.578 ± 0.383
4.812ValLeu: 4.812 ± 0.488
1.686ValMet: 1.686 ± 0.295
2.632ValAsn: 2.632 ± 0.309
3.702ValPro: 3.702 ± 0.392
3.331ValGln: 3.331 ± 0.379
5.223ValArg: 5.223 ± 0.475
3.496ValSer: 3.496 ± 0.354
5.881ValThr: 5.881 ± 0.483
6.169ValVal: 6.169 ± 0.612
1.604ValTrp: 1.604 ± 0.318
2.385ValTyr: 2.385 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
1.892TrpAla: 1.892 ± 0.243
0.247TrpCys: 0.247 ± 0.107
1.686TrpAsp: 1.686 ± 0.275
1.316TrpGlu: 1.316 ± 0.287
0.576TrpPhe: 0.576 ± 0.144
1.234TrpGly: 1.234 ± 0.235
0.494TrpHis: 0.494 ± 0.13
0.823TrpIle: 0.823 ± 0.154
0.699TrpLys: 0.699 ± 0.178
1.522TrpLeu: 1.522 ± 0.289
0.535TrpMet: 0.535 ± 0.126
0.658TrpAsn: 0.658 ± 0.248
0.74TrpPro: 0.74 ± 0.181
0.658TrpGln: 0.658 ± 0.16
2.139TrpArg: 2.139 ± 0.316
1.398TrpSer: 1.398 ± 0.241
1.439TrpThr: 1.439 ± 0.192
2.098TrpVal: 2.098 ± 0.335
0.494TrpTrp: 0.494 ± 0.152
0.617TrpTyr: 0.617 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.262TyrAla: 2.262 ± 0.268
0.288TyrCys: 0.288 ± 0.105
1.851TyrAsp: 1.851 ± 0.28
1.604TyrGlu: 1.604 ± 0.277
0.617TyrPhe: 0.617 ± 0.167
2.385TyrGly: 2.385 ± 0.297
0.452TyrHis: 0.452 ± 0.119
0.987TyrIle: 0.987 ± 0.179
1.152TyrLys: 1.152 ± 0.251
1.645TyrLeu: 1.645 ± 0.267
0.411TyrMet: 0.411 ± 0.145
0.905TyrAsn: 0.905 ± 0.216
1.275TyrPro: 1.275 ± 0.267
0.864TyrGln: 0.864 ± 0.212
1.933TyrArg: 1.933 ± 0.262
1.563TyrSer: 1.563 ± 0.285
1.686TyrThr: 1.686 ± 0.277
1.316TyrVal: 1.316 ± 0.239
0.699TyrTrp: 0.699 ± 0.142
0.699TyrTyr: 0.699 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 114 proteins (24315 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski