Amino acid dipepetide frequency for Mycobacterium phage Marshawn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.689AlaAla: 22.689 ± 1.727
0.852AlaCys: 0.852 ± 0.248
9.64AlaAsp: 9.64 ± 0.709
9.267AlaGlu: 9.267 ± 0.827
2.983AlaPhe: 2.983 ± 0.573
9.267AlaGly: 9.267 ± 1.02
2.556AlaHis: 2.556 ± 0.378
5.699AlaIle: 5.699 ± 0.765
4.314AlaLys: 4.314 ± 0.557
11.504AlaLeu: 11.504 ± 0.838
3.728AlaMet: 3.728 ± 0.405
2.929AlaAsn: 2.929 ± 0.365
7.084AlaPro: 7.084 ± 0.638
4.207AlaGln: 4.207 ± 0.612
9.107AlaArg: 9.107 ± 0.845
5.965AlaSer: 5.965 ± 0.531
6.391AlaThr: 6.391 ± 0.667
10.758AlaVal: 10.758 ± 0.757
2.556AlaTrp: 2.556 ± 0.341
2.876AlaTyr: 2.876 ± 0.481
0.0AlaXaa: 0.0 ± 0.0
Cys
1.438CysAla: 1.438 ± 0.318
0.053CysCys: 0.053 ± 0.054
0.639CysAsp: 0.639 ± 0.213
0.586CysGlu: 0.586 ± 0.215
0.213CysPhe: 0.213 ± 0.105
1.278CysGly: 1.278 ± 0.305
0.32CysHis: 0.32 ± 0.146
0.213CysIle: 0.213 ± 0.091
0.426CysLys: 0.426 ± 0.182
0.746CysLeu: 0.746 ± 0.216
0.213CysMet: 0.213 ± 0.118
0.373CysAsn: 0.373 ± 0.15
0.799CysPro: 0.799 ± 0.233
0.32CysGln: 0.32 ± 0.133
0.692CysArg: 0.692 ± 0.18
0.905CysSer: 0.905 ± 0.215
0.799CysThr: 0.799 ± 0.239
0.479CysVal: 0.479 ± 0.144
0.426CysTrp: 0.426 ± 0.17
0.053CysTyr: 0.053 ± 0.054
0.0CysXaa: 0.0 ± 0.0
Asp
7.936AspAla: 7.936 ± 0.667
0.586AspCys: 0.586 ± 0.179
4.953AspAsp: 4.953 ± 0.624
6.178AspGlu: 6.178 ± 0.689
1.172AspPhe: 1.172 ± 0.206
7.03AspGly: 7.03 ± 0.649
0.959AspHis: 0.959 ± 0.215
1.491AspIle: 1.491 ± 0.265
1.545AspLys: 1.545 ± 0.262
5.592AspLeu: 5.592 ± 0.665
2.077AspMet: 2.077 ± 0.376
1.811AspAsn: 1.811 ± 0.339
4.048AspPro: 4.048 ± 0.458
1.811AspGln: 1.811 ± 0.266
5.219AspArg: 5.219 ± 0.574
2.769AspSer: 2.769 ± 0.365
3.409AspThr: 3.409 ± 0.366
5.326AspVal: 5.326 ± 0.557
0.746AspTrp: 0.746 ± 0.206
1.758AspTyr: 1.758 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
8.149GluAla: 8.149 ± 0.849
1.012GluCys: 1.012 ± 0.262
2.769GluAsp: 2.769 ± 0.417
1.012GluGlu: 1.012 ± 0.267
2.184GluPhe: 2.184 ± 0.283
4.74GluGly: 4.74 ± 0.643
2.024GluHis: 2.024 ± 0.394
2.343GluIle: 2.343 ± 0.354
1.491GluLys: 1.491 ± 0.311
6.018GluLeu: 6.018 ± 0.468
1.065GluMet: 1.065 ± 0.243
0.905GluAsn: 0.905 ± 0.226
2.61GluPro: 2.61 ± 0.52
2.503GluGln: 2.503 ± 0.376
5.006GluArg: 5.006 ± 0.498
1.758GluSer: 1.758 ± 0.283
2.503GluThr: 2.503 ± 0.328
5.273GluVal: 5.273 ± 0.706
1.172GluTrp: 1.172 ± 0.339
1.811GluTyr: 1.811 ± 0.337
0.0GluXaa: 0.0 ± 0.0
Phe
2.929PheAla: 2.929 ± 0.447
0.16PheCys: 0.16 ± 0.089
3.036PheAsp: 3.036 ± 0.439
1.065PheGlu: 1.065 ± 0.223
0.746PhePhe: 0.746 ± 0.222
2.929PheGly: 2.929 ± 0.352
0.426PheHis: 0.426 ± 0.137
0.852PheIle: 0.852 ± 0.2
1.172PheLys: 1.172 ± 0.318
2.29PheLeu: 2.29 ± 0.352
0.799PheMet: 0.799 ± 0.206
0.959PheAsn: 0.959 ± 0.217
0.959PhePro: 0.959 ± 0.244
0.692PheGln: 0.692 ± 0.189
1.438PheArg: 1.438 ± 0.261
1.172PheSer: 1.172 ± 0.281
2.184PheThr: 2.184 ± 0.357
2.716PheVal: 2.716 ± 0.436
0.373PheTrp: 0.373 ± 0.135
0.426PheTyr: 0.426 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
10.119GlyAla: 10.119 ± 1.13
0.959GlyCys: 0.959 ± 0.244
4.9GlyAsp: 4.9 ± 0.529
4.847GlyGlu: 4.847 ± 0.563
2.983GlyPhe: 2.983 ± 0.454
9.853GlyGly: 9.853 ± 1.571
1.864GlyHis: 1.864 ± 0.357
2.769GlyIle: 2.769 ± 0.44
3.994GlyLys: 3.994 ± 0.564
7.829GlyLeu: 7.829 ± 0.898
1.545GlyMet: 1.545 ± 0.248
2.716GlyAsn: 2.716 ± 0.385
3.249GlyPro: 3.249 ± 0.45
2.556GlyGln: 2.556 ± 0.394
6.231GlyArg: 6.231 ± 0.546
5.379GlySer: 5.379 ± 0.563
6.018GlyThr: 6.018 ± 0.635
7.137GlyVal: 7.137 ± 0.563
2.397GlyTrp: 2.397 ± 0.335
2.077GlyTyr: 2.077 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
2.663HisAla: 2.663 ± 0.396
0.32HisCys: 0.32 ± 0.129
1.704HisAsp: 1.704 ± 0.374
1.598HisGlu: 1.598 ± 0.275
0.266HisPhe: 0.266 ± 0.116
2.024HisGly: 2.024 ± 0.359
0.426HisHis: 0.426 ± 0.14
0.692HisIle: 0.692 ± 0.239
0.373HisLys: 0.373 ± 0.139
2.45HisLeu: 2.45 ± 0.386
0.16HisMet: 0.16 ± 0.088
0.852HisAsn: 0.852 ± 0.22
1.491HisPro: 1.491 ± 0.287
0.213HisGln: 0.213 ± 0.097
1.917HisArg: 1.917 ± 0.397
1.012HisSer: 1.012 ± 0.264
1.438HisThr: 1.438 ± 0.222
1.598HisVal: 1.598 ± 0.267
0.586HisTrp: 0.586 ± 0.232
0.479HisTyr: 0.479 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
4.953IleAla: 4.953 ± 0.511
0.266IleCys: 0.266 ± 0.105
2.983IleAsp: 2.983 ± 0.378
3.196IleGlu: 3.196 ± 0.371
0.479IlePhe: 0.479 ± 0.166
4.207IleGly: 4.207 ± 0.822
0.639IleHis: 0.639 ± 0.184
1.172IleIle: 1.172 ± 0.214
1.598IleLys: 1.598 ± 0.355
1.971IleLeu: 1.971 ± 0.322
0.426IleMet: 0.426 ± 0.143
1.278IleAsn: 1.278 ± 0.239
2.29IlePro: 2.29 ± 0.429
0.692IleGln: 0.692 ± 0.149
2.077IleArg: 2.077 ± 0.315
1.491IleSer: 1.491 ± 0.233
2.716IleThr: 2.716 ± 0.316
3.462IleVal: 3.462 ± 0.414
0.692IleTrp: 0.692 ± 0.233
0.266IleTyr: 0.266 ± 0.146
0.0IleXaa: 0.0 ± 0.0
Lys
3.089LysAla: 3.089 ± 0.4
0.32LysCys: 0.32 ± 0.123
1.225LysAsp: 1.225 ± 0.247
0.799LysGlu: 0.799 ± 0.159
1.065LysPhe: 1.065 ± 0.211
3.142LysGly: 3.142 ± 0.463
0.692LysHis: 0.692 ± 0.186
1.491LysIle: 1.491 ± 0.312
0.639LysLys: 0.639 ± 0.164
2.716LysLeu: 2.716 ± 0.318
0.746LysMet: 0.746 ± 0.209
0.533LysAsn: 0.533 ± 0.163
3.142LysPro: 3.142 ± 0.485
0.852LysGln: 0.852 ± 0.205
2.769LysArg: 2.769 ± 0.498
0.852LysSer: 0.852 ± 0.199
2.397LysThr: 2.397 ± 0.431
2.503LysVal: 2.503 ± 0.42
0.586LysTrp: 0.586 ± 0.121
0.852LysTyr: 0.852 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
13.528LeuAla: 13.528 ± 0.919
0.852LeuCys: 0.852 ± 0.218
7.723LeuAsp: 7.723 ± 0.533
1.971LeuGlu: 1.971 ± 0.44
2.61LeuPhe: 2.61 ± 0.437
7.297LeuGly: 7.297 ± 0.599
1.651LeuHis: 1.651 ± 0.334
3.409LeuIle: 3.409 ± 0.333
2.077LeuLys: 2.077 ± 0.322
6.498LeuLeu: 6.498 ± 0.589
2.184LeuMet: 2.184 ± 0.301
2.45LeuAsn: 2.45 ± 0.384
4.314LeuPro: 4.314 ± 0.468
2.61LeuGln: 2.61 ± 0.366
6.551LeuArg: 6.551 ± 0.6
5.113LeuSer: 5.113 ± 0.458
4.687LeuThr: 4.687 ± 0.525
5.646LeuVal: 5.646 ± 0.54
1.491LeuTrp: 1.491 ± 0.268
1.385LeuTyr: 1.385 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
3.568MetAla: 3.568 ± 0.374
0.213MetCys: 0.213 ± 0.111
0.959MetAsp: 0.959 ± 0.222
0.639MetGlu: 0.639 ± 0.249
0.905MetPhe: 0.905 ± 0.19
1.758MetGly: 1.758 ± 0.284
0.533MetHis: 0.533 ± 0.152
1.118MetIle: 1.118 ± 0.222
0.373MetLys: 0.373 ± 0.137
1.438MetLeu: 1.438 ± 0.328
0.266MetMet: 0.266 ± 0.112
0.533MetAsn: 0.533 ± 0.151
1.598MetPro: 1.598 ± 0.281
0.905MetGln: 0.905 ± 0.216
1.491MetArg: 1.491 ± 0.3
2.024MetSer: 2.024 ± 0.282
2.077MetThr: 2.077 ± 0.335
1.651MetVal: 1.651 ± 0.332
0.533MetTrp: 0.533 ± 0.157
0.479MetTyr: 0.479 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
3.462AsnAla: 3.462 ± 0.396
0.266AsnCys: 0.266 ± 0.117
1.545AsnAsp: 1.545 ± 0.304
0.852AsnGlu: 0.852 ± 0.17
0.746AsnPhe: 0.746 ± 0.184
3.622AsnGly: 3.622 ± 0.386
0.479AsnHis: 0.479 ± 0.144
0.799AsnIle: 0.799 ± 0.21
0.852AsnLys: 0.852 ± 0.214
1.971AsnLeu: 1.971 ± 0.335
0.533AsnMet: 0.533 ± 0.161
0.533AsnAsn: 0.533 ± 0.171
2.077AsnPro: 2.077 ± 0.333
0.426AsnGln: 0.426 ± 0.17
1.917AsnArg: 1.917 ± 0.278
0.533AsnSer: 0.533 ± 0.16
1.278AsnThr: 1.278 ± 0.205
2.663AsnVal: 2.663 ± 0.382
0.32AsnTrp: 0.32 ± 0.132
0.586AsnTyr: 0.586 ± 0.147
0.0AsnXaa: 0.0 ± 0.0
Pro
8.468ProAla: 8.468 ± 0.714
0.426ProCys: 0.426 ± 0.147
3.888ProAsp: 3.888 ± 0.392
5.219ProGlu: 5.219 ± 0.54
1.545ProPhe: 1.545 ± 0.265
5.113ProGly: 5.113 ± 0.562
1.118ProHis: 1.118 ± 0.318
1.704ProIle: 1.704 ± 0.321
1.704ProLys: 1.704 ± 0.321
4.314ProLeu: 4.314 ± 0.555
1.172ProMet: 1.172 ± 0.246
1.118ProAsn: 1.118 ± 0.384
2.983ProPro: 2.983 ± 0.402
1.385ProGln: 1.385 ± 0.284
3.142ProArg: 3.142 ± 0.503
2.61ProSer: 2.61 ± 0.334
3.249ProThr: 3.249 ± 0.417
5.06ProVal: 5.06 ± 0.508
0.959ProTrp: 0.959 ± 0.198
1.331ProTyr: 1.331 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
5.006GlnAla: 5.006 ± 0.559
0.479GlnCys: 0.479 ± 0.169
1.118GlnAsp: 1.118 ± 0.222
1.012GlnGlu: 1.012 ± 0.252
0.746GlnPhe: 0.746 ± 0.21
2.556GlnGly: 2.556 ± 0.369
0.852GlnHis: 0.852 ± 0.172
1.758GlnIle: 1.758 ± 0.317
0.692GlnLys: 0.692 ± 0.206
2.769GlnLeu: 2.769 ± 0.467
1.172GlnMet: 1.172 ± 0.268
0.799GlnAsn: 0.799 ± 0.186
1.864GlnPro: 1.864 ± 0.238
1.172GlnGln: 1.172 ± 0.304
2.503GlnArg: 2.503 ± 0.321
1.225GlnSer: 1.225 ± 0.253
1.651GlnThr: 1.651 ± 0.293
2.184GlnVal: 2.184 ± 0.3
0.959GlnTrp: 0.959 ± 0.313
0.852GlnTyr: 0.852 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
7.403ArgAla: 7.403 ± 0.678
1.385ArgCys: 1.385 ± 0.237
4.367ArgAsp: 4.367 ± 0.525
4.527ArgGlu: 4.527 ± 0.475
1.811ArgPhe: 1.811 ± 0.325
5.219ArgGly: 5.219 ± 0.603
2.184ArgHis: 2.184 ± 0.443
2.823ArgIle: 2.823 ± 0.508
2.929ArgLys: 2.929 ± 0.377
6.338ArgLeu: 6.338 ± 0.679
2.237ArgMet: 2.237 ± 0.321
2.29ArgAsn: 2.29 ± 0.337
3.355ArgPro: 3.355 ± 0.578
3.142ArgGln: 3.142 ± 0.329
6.125ArgArg: 6.125 ± 0.73
3.196ArgSer: 3.196 ± 0.401
3.781ArgThr: 3.781 ± 0.533
4.687ArgVal: 4.687 ± 0.504
1.864ArgTrp: 1.864 ± 0.303
2.397ArgTyr: 2.397 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
6.285SerAla: 6.285 ± 0.78
0.16SerCys: 0.16 ± 0.09
2.45SerAsp: 2.45 ± 0.437
1.864SerGlu: 1.864 ± 0.28
1.811SerPhe: 1.811 ± 0.249
4.474SerGly: 4.474 ± 0.682
1.225SerHis: 1.225 ± 0.283
2.13SerIle: 2.13 ± 0.342
1.331SerLys: 1.331 ± 0.205
3.409SerLeu: 3.409 ± 0.329
1.545SerMet: 1.545 ± 0.323
0.905SerAsn: 0.905 ± 0.219
3.409SerPro: 3.409 ± 0.402
1.864SerGln: 1.864 ± 0.283
2.876SerArg: 2.876 ± 0.322
2.876SerSer: 2.876 ± 0.386
3.622SerThr: 3.622 ± 0.583
3.835SerVal: 3.835 ± 0.404
1.331SerTrp: 1.331 ± 0.242
1.385SerTyr: 1.385 ± 0.249
0.0SerXaa: 0.0 ± 0.0
Thr
7.03ThrAla: 7.03 ± 0.662
1.065ThrCys: 1.065 ± 0.273
3.781ThrAsp: 3.781 ± 0.379
3.462ThrGlu: 3.462 ± 0.421
1.864ThrPhe: 1.864 ± 0.316
5.539ThrGly: 5.539 ± 0.534
1.278ThrHis: 1.278 ± 0.31
2.876ThrIle: 2.876 ± 0.388
1.704ThrLys: 1.704 ± 0.335
5.379ThrLeu: 5.379 ± 0.448
0.639ThrMet: 0.639 ± 0.202
1.651ThrAsn: 1.651 ± 0.256
4.101ThrPro: 4.101 ± 0.4
1.864ThrGln: 1.864 ± 0.351
2.929ThrArg: 2.929 ± 0.502
3.249ThrSer: 3.249 ± 0.452
4.314ThrThr: 4.314 ± 0.542
4.9ThrVal: 4.9 ± 0.481
0.905ThrTrp: 0.905 ± 0.226
1.491ThrTyr: 1.491 ± 0.278
0.0ThrXaa: 0.0 ± 0.0
Val
10.758ValAla: 10.758 ± 1.007
0.959ValCys: 0.959 ± 0.209
5.752ValAsp: 5.752 ± 0.51
5.646ValGlu: 5.646 ± 0.607
1.971ValPhe: 1.971 ± 0.367
5.912ValGly: 5.912 ± 0.67
2.237ValHis: 2.237 ± 0.399
2.343ValIle: 2.343 ± 0.371
2.503ValLys: 2.503 ± 0.307
6.231ValLeu: 6.231 ± 0.569
1.651ValMet: 1.651 ± 0.256
1.651ValAsn: 1.651 ± 0.243
5.486ValPro: 5.486 ± 0.661
2.503ValGln: 2.503 ± 0.31
5.326ValArg: 5.326 ± 0.593
3.781ValSer: 3.781 ± 0.435
5.273ValThr: 5.273 ± 0.445
6.391ValVal: 6.391 ± 0.766
2.13ValTrp: 2.13 ± 0.326
1.758ValTyr: 1.758 ± 0.227
0.0ValXaa: 0.0 ± 0.0
Trp
2.45TrpAla: 2.45 ± 0.352
0.266TrpCys: 0.266 ± 0.121
0.959TrpAsp: 0.959 ± 0.233
0.799TrpGlu: 0.799 ± 0.234
0.692TrpPhe: 0.692 ± 0.195
1.438TrpGly: 1.438 ± 0.256
0.426TrpHis: 0.426 ± 0.164
0.533TrpIle: 0.533 ± 0.159
0.266TrpLys: 0.266 ± 0.108
2.61TrpLeu: 2.61 ± 0.432
0.426TrpMet: 0.426 ± 0.136
0.639TrpAsn: 0.639 ± 0.187
0.639TrpPro: 0.639 ± 0.151
1.065TrpGln: 1.065 ± 0.231
2.716TrpArg: 2.716 ± 0.51
1.331TrpSer: 1.331 ± 0.317
0.746TrpThr: 0.746 ± 0.187
1.971TrpVal: 1.971 ± 0.344
0.32TrpTrp: 0.32 ± 0.101
0.479TrpTyr: 0.479 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.45TyrAla: 2.45 ± 0.368
0.373TyrCys: 0.373 ± 0.131
2.024TyrAsp: 2.024 ± 0.364
1.811TyrGlu: 1.811 ± 0.285
0.533TyrPhe: 0.533 ± 0.167
2.024TyrGly: 2.024 ± 0.323
0.373TyrHis: 0.373 ± 0.111
0.639TyrIle: 0.639 ± 0.229
0.479TyrLys: 0.479 ± 0.191
1.971TyrLeu: 1.971 ± 0.39
0.426TyrMet: 0.426 ± 0.119
0.533TyrAsn: 0.533 ± 0.159
1.012TyrPro: 1.012 ± 0.214
0.586TyrGln: 0.586 ± 0.214
2.024TyrArg: 2.024 ± 0.387
1.545TyrSer: 1.545 ± 0.254
1.491TyrThr: 1.491 ± 0.264
2.024TyrVal: 2.024 ± 0.368
0.426TyrTrp: 0.426 ± 0.178
0.533TyrTyr: 0.533 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (18777 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski