Halovirus VNH-1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 4.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068IVG8|A0A068IVG8_9VIRU NAD dependent epimerase OS=Halovirus VNH-1 OX=1500510 PE=4 SV=1
MM1 pKa = 7.6SNEE4 pKa = 3.83DD5 pKa = 4.62CEE7 pKa = 4.67TKK9 pKa = 10.8DD10 pKa = 3.55SIEE13 pKa = 4.16TRR15 pKa = 11.84TEE17 pKa = 3.79RR18 pKa = 11.84ALTEE22 pKa = 4.24CMTVLPDD29 pKa = 3.39HH30 pKa = 6.69GRR32 pKa = 11.84AEE34 pKa = 4.26DD35 pKa = 3.82APGLFVVVGEE45 pKa = 4.36NCNGEE50 pKa = 4.31YY51 pKa = 10.67LVDD54 pKa = 3.85TRR56 pKa = 11.84TEE58 pKa = 3.85SCEE61 pKa = 4.24CKK63 pKa = 9.81DD64 pKa = 3.3AKK66 pKa = 10.95YY67 pKa = 10.34RR68 pKa = 11.84DD69 pKa = 3.79PEE71 pKa = 4.64GGCKK75 pKa = 10.06HH76 pKa = 6.08IRR78 pKa = 11.84RR79 pKa = 11.84CRR81 pKa = 11.84IAQGEE86 pKa = 4.59TPVPAGALGEE96 pKa = 4.08ITIDD100 pKa = 3.57STFGAQLEE108 pKa = 4.63TSAKK112 pKa = 9.95FATADD117 pKa = 3.34GGIIDD122 pKa = 4.58AEE124 pKa = 4.32SGEE127 pKa = 4.44KK128 pKa = 9.95ISDD131 pKa = 3.64EE132 pKa = 4.51TEE134 pKa = 4.21STTSWSDD141 pKa = 3.12PMAEE145 pKa = 3.79TDD147 pKa = 4.45KK148 pKa = 11.26YY149 pKa = 11.08GKK151 pKa = 8.14PTGDD155 pKa = 3.65HH156 pKa = 6.51YY157 pKa = 10.2VTCQEE162 pKa = 4.62CGIEE166 pKa = 4.3VLTALADD173 pKa = 3.92CATHH177 pKa = 7.66RR178 pKa = 11.84EE179 pKa = 4.57GCSEE183 pKa = 3.94

Molecular weight: 19.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068IVW4|A0A068IVW4_9VIRU Hypothetical conserved protein OS=Halovirus VNH-1 OX=1500510 PE=4 SV=1
MM1 pKa = 7.48SIKK4 pKa = 10.69EE5 pKa = 4.08NTLTGAPEE13 pKa = 4.02TVDD16 pKa = 3.12EE17 pKa = 4.74HH18 pKa = 8.83EE19 pKa = 4.66EE20 pKa = 3.92MIQILDD26 pKa = 3.7EE27 pKa = 5.12LISEE31 pKa = 4.84GLRR34 pKa = 11.84KK35 pKa = 8.64FTGDD39 pKa = 3.64GRR41 pKa = 11.84IRR43 pKa = 11.84DD44 pKa = 3.78NKK46 pKa = 9.46KK47 pKa = 8.95EE48 pKa = 4.01KK49 pKa = 10.67VRR51 pKa = 11.84IQYY54 pKa = 7.35MKK56 pKa = 9.78RR57 pKa = 11.84TEE59 pKa = 3.68QAIRR63 pKa = 11.84AKK65 pKa = 10.35RR66 pKa = 11.84QVVKK70 pKa = 10.68DD71 pKa = 3.61KK72 pKa = 10.86QLQQMGRR79 pKa = 11.84ALEE82 pKa = 4.1RR83 pKa = 11.84LQEE86 pKa = 4.23EE87 pKa = 4.23NDD89 pKa = 3.4LDD91 pKa = 4.97LNLSNN96 pKa = 5.17

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1191

75

489

170.1

18.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.137 ± 0.682

1.679 ± 0.961

8.984 ± 1.025

10.243 ± 1.502

1.847 ± 0.52

8.48 ± 1.0

1.511 ± 0.257

5.122 ± 0.727

4.282 ± 0.586

6.969 ± 0.806

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.931 ± 0.594

3.442 ± 0.661

3.023 ± 0.436

4.03 ± 0.864

6.801 ± 0.487

6.969 ± 0.894

7.473 ± 1.553

5.793 ± 0.591

1.259 ± 0.407

3.023 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski