Janibacter melonis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Janibacter

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A176QGB6|A0A176QGB6_9MICO MarR family transcriptional regulator OS=Janibacter melonis OX=262209 GN=AWH69_02935 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84APLALLAVPALALALASCGSDD23 pKa = 3.06EE24 pKa = 4.61PEE26 pKa = 4.21NPTGVSQSGAAVEE39 pKa = 4.44CPPAEE44 pKa = 4.15GVEE47 pKa = 4.52TPVQTFEE54 pKa = 4.35QAPPMCIDD62 pKa = 3.31EE63 pKa = 4.76AKK65 pKa = 10.03TYY67 pKa = 8.11TAVMTTDD74 pKa = 3.08VGDD77 pKa = 3.42VTFALDD83 pKa = 3.39AKK85 pKa = 10.36KK86 pKa = 10.75APTTVNNFVVLSRR99 pKa = 11.84YY100 pKa = 9.66GYY102 pKa = 8.33YY103 pKa = 10.64DD104 pKa = 3.33GVPFHH109 pKa = 6.86RR110 pKa = 11.84VIPGFMIQGGDD121 pKa = 3.34PTGQGNGGPGYY132 pKa = 10.04EE133 pKa = 5.08FEE135 pKa = 5.92DD136 pKa = 4.05EE137 pKa = 4.31LPAAGEE143 pKa = 4.17YY144 pKa = 10.48EE145 pKa = 4.17IGSLAMANAGPDD157 pKa = 3.6TNGSQFFVVTGDD169 pKa = 3.48SGVTLPPDD177 pKa = 3.2YY178 pKa = 11.4SLFGKK183 pKa = 7.95VTSGMEE189 pKa = 3.9TVRR192 pKa = 11.84EE193 pKa = 4.0IEE195 pKa = 4.03EE196 pKa = 4.83DD197 pKa = 3.72GSADD201 pKa = 3.47GSPGEE206 pKa = 3.92QHH208 pKa = 6.79TIDD211 pKa = 4.89SITITEE217 pKa = 4.2KK218 pKa = 10.98

Molecular weight: 22.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A176QFS7|A0A176QFS7_9MICO Chromosomal replication initiator protein DnaA OS=Janibacter melonis OX=262209 GN=dnaA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.45GRR40 pKa = 11.84AKK42 pKa = 10.69LSAA45 pKa = 3.92

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2936

0

2936

947235

29

1599

322.6

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.082 ± 0.058

0.671 ± 0.013

6.48 ± 0.04

5.912 ± 0.04

2.404 ± 0.03

9.52 ± 0.04

2.251 ± 0.022

3.084 ± 0.033

1.698 ± 0.033

10.215 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.888 ± 0.017

1.305 ± 0.02

5.668 ± 0.034

2.861 ± 0.023

7.914 ± 0.045

5.432 ± 0.032

6.116 ± 0.039

10.144 ± 0.046

1.495 ± 0.018

1.858 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski