Amino acid dipepetide frequency for Ruminococcus sp. AM42-11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.067AlaAla: 7.067 ± 0.097
1.069AlaCys: 1.069 ± 0.033
4.757AlaAsp: 4.757 ± 0.078
5.428AlaGlu: 5.428 ± 0.076
2.88AlaPhe: 2.88 ± 0.063
5.838AlaGly: 5.838 ± 0.084
1.077AlaHis: 1.077 ± 0.033
4.631AlaIle: 4.631 ± 0.068
4.774AlaLys: 4.774 ± 0.081
6.405AlaLeu: 6.405 ± 0.082
2.338AlaMet: 2.338 ± 0.05
2.571AlaAsn: 2.571 ± 0.047
2.007AlaPro: 2.007 ± 0.056
2.322AlaGln: 2.322 ± 0.045
2.882AlaArg: 2.882 ± 0.058
3.968AlaSer: 3.968 ± 0.067
3.281AlaThr: 3.281 ± 0.065
6.111AlaVal: 6.111 ± 0.092
0.614AlaTrp: 0.614 ± 0.024
2.723AlaTyr: 2.723 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.989CysAla: 0.989 ± 0.028
0.323CysCys: 0.323 ± 0.02
0.87CysAsp: 0.87 ± 0.029
0.933CysGlu: 0.933 ± 0.03
0.644CysPhe: 0.644 ± 0.025
1.555CysGly: 1.555 ± 0.049
0.341CysHis: 0.341 ± 0.017
1.144CysIle: 1.144 ± 0.036
0.973CysLys: 0.973 ± 0.03
1.209CysLeu: 1.209 ± 0.033
0.491CysMet: 0.491 ± 0.022
0.643CysAsn: 0.643 ± 0.024
0.65CysPro: 0.65 ± 0.028
0.547CysGln: 0.547 ± 0.023
0.742CysArg: 0.742 ± 0.027
0.938CysSer: 0.938 ± 0.03
0.893CysThr: 0.893 ± 0.032
0.981CysVal: 0.981 ± 0.028
0.142CysTrp: 0.142 ± 0.011
0.636CysTyr: 0.636 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.932AspAla: 3.932 ± 0.072
0.88AspCys: 0.88 ± 0.026
3.082AspAsp: 3.082 ± 0.063
4.794AspGlu: 4.794 ± 0.078
2.734AspPhe: 2.734 ± 0.046
4.751AspGly: 4.751 ± 0.083
0.852AspHis: 0.852 ± 0.03
4.279AspIle: 4.279 ± 0.07
3.644AspLys: 3.644 ± 0.056
4.532AspLeu: 4.532 ± 0.058
1.931AspMet: 1.931 ± 0.039
2.411AspAsn: 2.411 ± 0.05
1.784AspPro: 1.784 ± 0.04
1.641AspGln: 1.641 ± 0.041
2.201AspArg: 2.201 ± 0.045
3.424AspSer: 3.424 ± 0.077
3.258AspThr: 3.258 ± 0.061
3.764AspVal: 3.764 ± 0.058
0.646AspTrp: 0.646 ± 0.025
2.858AspTyr: 2.858 ± 0.055
0.0AspXaa: 0.0 ± 0.0
Glu
5.548GluAla: 5.548 ± 0.082
0.921GluCys: 0.921 ± 0.031
4.504GluAsp: 4.504 ± 0.074
7.248GluGlu: 7.248 ± 0.113
2.717GluPhe: 2.717 ± 0.055
4.242GluGly: 4.242 ± 0.065
1.255GluHis: 1.255 ± 0.037
5.694GluIle: 5.694 ± 0.078
7.344GluLys: 7.344 ± 0.099
6.601GluLeu: 6.601 ± 0.08
2.489GluMet: 2.489 ± 0.041
4.737GluAsn: 4.737 ± 0.063
1.874GluPro: 1.874 ± 0.05
2.84GluGln: 2.84 ± 0.058
3.071GluArg: 3.071 ± 0.066
3.43GluSer: 3.43 ± 0.053
3.952GluThr: 3.952 ± 0.075
4.423GluVal: 4.423 ± 0.072
0.65GluTrp: 0.65 ± 0.025
3.219GluTyr: 3.219 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
2.845PheAla: 2.845 ± 0.056
0.834PheCys: 0.834 ± 0.029
2.449PheAsp: 2.449 ± 0.052
2.604PheGlu: 2.604 ± 0.049
1.828PhePhe: 1.828 ± 0.047
2.995PheGly: 2.995 ± 0.051
0.894PheHis: 0.894 ± 0.028
2.617PheIle: 2.617 ± 0.052
1.994PheLys: 1.994 ± 0.039
3.87PheLeu: 3.87 ± 0.076
1.17PheMet: 1.17 ± 0.031
1.535PheAsn: 1.535 ± 0.037
1.414PhePro: 1.414 ± 0.035
1.453PheGln: 1.453 ± 0.037
1.666PheArg: 1.666 ± 0.041
2.927PheSer: 2.927 ± 0.054
2.501PheThr: 2.501 ± 0.048
2.695PheVal: 2.695 ± 0.058
0.502PheTrp: 0.502 ± 0.023
1.885PheTyr: 1.885 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.764GlyAla: 4.764 ± 0.085
1.247GlyCys: 1.247 ± 0.038
3.529GlyAsp: 3.529 ± 0.058
4.812GlyGlu: 4.812 ± 0.07
3.035GlyPhe: 3.035 ± 0.059
4.422GlyGly: 4.422 ± 0.09
1.244GlyHis: 1.244 ± 0.04
6.004GlyIle: 6.004 ± 0.086
5.948GlyLys: 5.948 ± 0.071
5.452GlyLeu: 5.452 ± 0.072
2.426GlyMet: 2.426 ± 0.045
3.461GlyAsn: 3.461 ± 0.065
1.176GlyPro: 1.176 ± 0.034
2.101GlyGln: 2.101 ± 0.04
2.908GlyArg: 2.908 ± 0.055
4.091GlySer: 4.091 ± 0.068
4.346GlyThr: 4.346 ± 0.065
4.696GlyVal: 4.696 ± 0.079
0.776GlyTrp: 0.776 ± 0.03
3.453GlyTyr: 3.453 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.07HisAla: 1.07 ± 0.028
0.332HisCys: 0.332 ± 0.017
0.857HisAsp: 0.857 ± 0.028
1.072HisGlu: 1.072 ± 0.031
0.845HisPhe: 0.845 ± 0.028
1.222HisGly: 1.222 ± 0.038
0.376HisHis: 0.376 ± 0.023
1.315HisIle: 1.315 ± 0.035
1.038HisLys: 1.038 ± 0.029
1.426HisLeu: 1.426 ± 0.038
0.583HisMet: 0.583 ± 0.021
0.752HisAsn: 0.752 ± 0.025
0.882HisPro: 0.882 ± 0.028
0.589HisGln: 0.589 ± 0.024
0.762HisArg: 0.762 ± 0.026
0.949HisSer: 0.949 ± 0.03
0.996HisThr: 0.996 ± 0.033
1.131HisVal: 1.131 ± 0.029
0.181HisTrp: 0.181 ± 0.011
0.817HisTyr: 0.817 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
5.252IleAla: 5.252 ± 0.077
1.35IleCys: 1.35 ± 0.04
3.712IleAsp: 3.712 ± 0.061
4.323IleGlu: 4.323 ± 0.059
2.945IlePhe: 2.945 ± 0.056
4.789IleGly: 4.789 ± 0.075
1.374IleHis: 1.374 ± 0.036
4.525IleIle: 4.525 ± 0.077
4.043IleLys: 4.043 ± 0.055
7.001IleLeu: 7.001 ± 0.094
1.878IleMet: 1.878 ± 0.044
2.799IleAsn: 2.799 ± 0.047
3.104IlePro: 3.104 ± 0.051
2.538IleGln: 2.538 ± 0.045
3.53IleArg: 3.53 ± 0.062
4.902IleSer: 4.902 ± 0.069
4.259IleThr: 4.259 ± 0.061
4.438IleVal: 4.438 ± 0.062
0.765IleTrp: 0.765 ± 0.023
2.915IleTyr: 2.915 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
5.199LysAla: 5.199 ± 0.069
0.864LysCys: 0.864 ± 0.027
4.101LysAsp: 4.101 ± 0.075
6.935LysGlu: 6.935 ± 0.094
2.177LysPhe: 2.177 ± 0.043
4.291LysGly: 4.291 ± 0.072
1.002LysHis: 1.002 ± 0.033
5.015LysIle: 5.015 ± 0.066
7.045LysLys: 7.045 ± 0.099
5.708LysLeu: 5.708 ± 0.068
2.427LysMet: 2.427 ± 0.045
3.891LysAsn: 3.891 ± 0.067
2.071LysPro: 2.071 ± 0.045
2.437LysGln: 2.437 ± 0.048
3.102LysArg: 3.102 ± 0.055
3.562LysSer: 3.562 ± 0.062
3.845LysThr: 3.845 ± 0.069
4.607LysVal: 4.607 ± 0.075
0.73LysTrp: 0.73 ± 0.028
3.195LysTyr: 3.195 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
6.481LeuAla: 6.481 ± 0.081
1.472LeuCys: 1.472 ± 0.042
5.137LeuAsp: 5.137 ± 0.076
6.45LeuGlu: 6.45 ± 0.082
3.683LeuPhe: 3.683 ± 0.064
5.739LeuGly: 5.739 ± 0.091
1.586LeuHis: 1.586 ± 0.043
5.622LeuIle: 5.622 ± 0.089
6.31LeuLys: 6.31 ± 0.07
8.383LeuLeu: 8.383 ± 0.112
2.545LeuMet: 2.545 ± 0.056
3.898LeuAsn: 3.898 ± 0.056
3.353LeuPro: 3.353 ± 0.051
2.934LeuGln: 2.934 ± 0.054
3.493LeuArg: 3.493 ± 0.063
6.004LeuSer: 6.004 ± 0.09
5.028LeuThr: 5.028 ± 0.064
5.465LeuVal: 5.465 ± 0.075
0.771LeuTrp: 0.771 ± 0.029
3.362LeuTyr: 3.362 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.496MetAla: 2.496 ± 0.049
0.401MetCys: 0.401 ± 0.018
1.92MetAsp: 1.92 ± 0.047
2.744MetGlu: 2.744 ± 0.055
1.009MetPhe: 1.009 ± 0.03
2.032MetGly: 2.032 ± 0.048
0.414MetHis: 0.414 ± 0.019
2.082MetIle: 2.082 ± 0.044
2.737MetLys: 2.737 ± 0.044
2.613MetLeu: 2.613 ± 0.045
0.93MetMet: 0.93 ± 0.026
1.614MetAsn: 1.614 ± 0.038
1.089MetPro: 1.089 ± 0.035
0.992MetGln: 0.992 ± 0.03
1.24MetArg: 1.24 ± 0.033
1.767MetSer: 1.767 ± 0.041
1.756MetThr: 1.756 ± 0.036
1.935MetVal: 1.935 ± 0.038
0.219MetTrp: 0.219 ± 0.014
0.909MetTyr: 0.909 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.143AsnAla: 3.143 ± 0.051
0.768AsnCys: 0.768 ± 0.028
2.218AsnAsp: 2.218 ± 0.042
2.895AsnGlu: 2.895 ± 0.057
1.662AsnPhe: 1.662 ± 0.039
3.988AsnGly: 3.988 ± 0.075
0.868AsnHis: 0.868 ± 0.027
3.338AsnIle: 3.338 ± 0.049
2.795AsnLys: 2.795 ± 0.046
4.033AsnLeu: 4.033 ± 0.066
1.437AsnMet: 1.437 ± 0.032
1.914AsnAsn: 1.914 ± 0.04
1.986AsnPro: 1.986 ± 0.045
1.607AsnGln: 1.607 ± 0.039
2.048AsnArg: 2.048 ± 0.045
2.597AsnSer: 2.597 ± 0.057
2.531AsnThr: 2.531 ± 0.055
2.952AsnVal: 2.952 ± 0.049
0.514AsnTrp: 0.514 ± 0.023
1.946AsnTyr: 1.946 ± 0.044
0.0AsnXaa: 0.0 ± 0.0
Pro
2.45ProAla: 2.45 ± 0.055
0.449ProCys: 0.449 ± 0.022
2.37ProAsp: 2.37 ± 0.051
3.383ProGlu: 3.383 ± 0.061
1.463ProPhe: 1.463 ± 0.042
2.334ProGly: 2.334 ± 0.046
0.499ProHis: 0.499 ± 0.021
1.853ProIle: 1.853 ± 0.042
1.912ProLys: 1.912 ± 0.042
2.612ProLeu: 2.612 ± 0.053
0.86ProMet: 0.86 ± 0.031
1.203ProAsn: 1.203 ± 0.032
0.656ProPro: 0.656 ± 0.024
1.064ProGln: 1.064 ± 0.03
1.01ProArg: 1.01 ± 0.027
1.707ProSer: 1.707 ± 0.04
1.736ProThr: 1.736 ± 0.048
3.032ProVal: 3.032 ± 0.05
0.324ProTrp: 0.324 ± 0.016
1.461ProTyr: 1.461 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
2.529GlnAla: 2.529 ± 0.051
0.37GlnCys: 0.37 ± 0.02
1.654GlnAsp: 1.654 ± 0.04
3.077GlnGlu: 3.077 ± 0.057
1.164GlnPhe: 1.164 ± 0.03
1.965GlnGly: 1.965 ± 0.048
0.48GlnHis: 0.48 ± 0.02
2.694GlnIle: 2.694 ± 0.046
3.067GlnLys: 3.067 ± 0.06
2.936GlnLeu: 2.936 ± 0.054
1.191GlnMet: 1.191 ± 0.035
1.811GlnAsn: 1.811 ± 0.043
0.999GlnPro: 0.999 ± 0.032
1.188GlnGln: 1.188 ± 0.036
1.392GlnArg: 1.392 ± 0.037
1.648GlnSer: 1.648 ± 0.037
1.868GlnThr: 1.868 ± 0.044
2.097GlnVal: 2.097 ± 0.045
0.327GlnTrp: 0.327 ± 0.018
1.394GlnTyr: 1.394 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
2.484ArgAla: 2.484 ± 0.041
0.623ArgCys: 0.623 ± 0.024
2.125ArgAsp: 2.125 ± 0.046
3.507ArgGlu: 3.507 ± 0.06
1.748ArgPhe: 1.748 ± 0.04
2.205ArgGly: 2.205 ± 0.042
0.829ArgHis: 0.829 ± 0.029
3.424ArgIle: 3.424 ± 0.062
3.765ArgLys: 3.765 ± 0.067
3.632ArgLeu: 3.632 ± 0.07
1.504ArgMet: 1.504 ± 0.037
2.135ArgAsn: 2.135 ± 0.041
1.204ArgPro: 1.204 ± 0.032
1.699ArgGln: 1.699 ± 0.044
1.993ArgArg: 1.993 ± 0.049
2.102ArgSer: 2.102 ± 0.048
2.199ArgThr: 2.199 ± 0.044
2.477ArgVal: 2.477 ± 0.054
0.41ArgTrp: 0.41 ± 0.021
1.932ArgTyr: 1.932 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
4.107SerAla: 4.107 ± 0.068
0.897SerCys: 0.897 ± 0.031
3.462SerAsp: 3.462 ± 0.073
4.005SerGlu: 4.005 ± 0.057
2.689SerPhe: 2.689 ± 0.052
4.906SerGly: 4.906 ± 0.078
1.058SerHis: 1.058 ± 0.034
3.915SerIle: 3.915 ± 0.061
3.382SerLys: 3.382 ± 0.058
5.118SerLeu: 5.118 ± 0.068
1.781SerMet: 1.781 ± 0.045
2.286SerAsn: 2.286 ± 0.052
1.694SerPro: 1.694 ± 0.038
2.102SerGln: 2.102 ± 0.051
2.71SerArg: 2.71 ± 0.055
3.616SerSer: 3.616 ± 0.074
2.983SerThr: 2.983 ± 0.061
4.364SerVal: 4.364 ± 0.061
0.693SerTrp: 0.693 ± 0.031
2.651SerTyr: 2.651 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.502ThrAla: 4.502 ± 0.081
0.765ThrCys: 0.765 ± 0.029
3.66ThrAsp: 3.66 ± 0.067
3.96ThrGlu: 3.96 ± 0.078
2.326ThrPhe: 2.326 ± 0.052
4.79ThrGly: 4.79 ± 0.073
0.84ThrHis: 0.84 ± 0.031
3.859ThrIle: 3.859 ± 0.061
3.113ThrLys: 3.113 ± 0.057
4.805ThrLeu: 4.805 ± 0.071
1.41ThrMet: 1.41 ± 0.035
2.061ThrAsn: 2.061 ± 0.045
2.261ThrPro: 2.261 ± 0.062
1.591ThrGln: 1.591 ± 0.035
2.12ThrArg: 2.12 ± 0.045
3.248ThrSer: 3.248 ± 0.061
2.974ThrThr: 2.974 ± 0.063
4.694ThrVal: 4.694 ± 0.092
0.574ThrTrp: 0.574 ± 0.022
2.277ThrTyr: 2.277 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
4.571ValAla: 4.571 ± 0.078
1.17ValCys: 1.17 ± 0.032
3.711ValAsp: 3.711 ± 0.066
4.609ValGlu: 4.609 ± 0.064
2.927ValPhe: 2.927 ± 0.052
4.113ValGly: 4.113 ± 0.061
1.087ValHis: 1.087 ± 0.03
4.951ValIle: 4.951 ± 0.073
4.878ValLys: 4.878 ± 0.072
6.517ValLeu: 6.517 ± 0.081
2.011ValMet: 2.011 ± 0.045
2.977ValAsn: 2.977 ± 0.052
2.501ValPro: 2.501 ± 0.045
2.156ValGln: 2.156 ± 0.043
2.694ValArg: 2.694 ± 0.052
4.488ValSer: 4.488 ± 0.068
4.412ValThr: 4.412 ± 0.084
4.611ValVal: 4.611 ± 0.085
0.647ValTrp: 0.647 ± 0.023
2.751ValTyr: 2.751 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.52TrpAla: 0.52 ± 0.024
0.175TrpCys: 0.175 ± 0.014
0.581TrpAsp: 0.581 ± 0.024
0.768TrpGlu: 0.768 ± 0.026
0.384TrpPhe: 0.384 ± 0.02
0.665TrpGly: 0.665 ± 0.027
0.23TrpHis: 0.23 ± 0.016
0.69TrpIle: 0.69 ± 0.024
0.911TrpLys: 0.911 ± 0.029
0.931TrpLeu: 0.931 ± 0.03
0.359TrpMet: 0.359 ± 0.019
0.668TrpAsn: 0.668 ± 0.026
0.202TrpPro: 0.202 ± 0.013
0.401TrpGln: 0.401 ± 0.018
0.385TrpArg: 0.385 ± 0.017
0.557TrpSer: 0.557 ± 0.024
0.504TrpThr: 0.504 ± 0.023
0.543TrpVal: 0.543 ± 0.023
0.116TrpTrp: 0.116 ± 0.011
0.43TrpTyr: 0.43 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.791TyrAla: 2.791 ± 0.056
0.736TyrCys: 0.736 ± 0.028
2.7TyrAsp: 2.7 ± 0.047
3.208TyrGlu: 3.208 ± 0.053
1.82TyrPhe: 1.82 ± 0.042
3.047TyrGly: 3.047 ± 0.053
0.89TyrHis: 0.89 ± 0.026
2.791TyrIle: 2.791 ± 0.055
2.532TyrLys: 2.532 ± 0.054
3.933TyrLeu: 3.933 ± 0.066
1.136TyrMet: 1.136 ± 0.031
1.875TyrAsn: 1.875 ± 0.041
1.471TyrPro: 1.471 ± 0.037
1.649TyrGln: 1.649 ± 0.04
2.047TyrArg: 2.047 ± 0.044
2.494TyrSer: 2.494 ± 0.051
2.507TyrThr: 2.507 ± 0.055
2.767TyrVal: 2.767 ± 0.059
0.418TyrTrp: 0.418 ± 0.019
2.103TyrTyr: 2.103 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3917 proteins (1165353 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski