Vibrio phage fs2

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Saetivirus; Vibrio virus fs2

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O80262|O80262_9VIRU Coat protein D OS=Vibrio phage fs2 OX=83201 PE=3 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84TVLTLFSTTLLLSISFSSFAYY23 pKa = 9.53YY24 pKa = 10.02QLPFWPDD31 pKa = 2.7KK32 pKa = 11.01TYY34 pKa = 9.5LTPEE38 pKa = 4.01AAAAAYY44 pKa = 10.57LDD46 pKa = 3.57ILGGGSCKK54 pKa = 9.81RR55 pKa = 11.84DD56 pKa = 2.96TRR58 pKa = 11.84NFIKK62 pKa = 10.75ASISSINTPHH72 pKa = 6.43IAYY75 pKa = 10.31RR76 pKa = 11.84IDD78 pKa = 4.32KK79 pKa = 10.47YY80 pKa = 11.39LDD82 pKa = 3.65DD83 pKa = 3.77QCIVLSSTGNASVTLNVVDD102 pKa = 4.11NCPDD106 pKa = 3.18GTSPDD111 pKa = 4.62LSTGMCKK118 pKa = 10.24PKK120 pKa = 10.12PDD122 pKa = 3.45TPQYY126 pKa = 10.28CGTSAMFEE134 pKa = 4.36DD135 pKa = 4.93VANLYY140 pKa = 7.91NACYY144 pKa = 8.22EE145 pKa = 4.14QNGILSYY152 pKa = 10.61TCDD155 pKa = 3.69EE156 pKa = 4.29STQALDD162 pKa = 3.92AKK164 pKa = 10.71CDD166 pKa = 3.69LTTSDD171 pKa = 3.0QCVIGRR177 pKa = 11.84PTWPDD182 pKa = 3.63CLDD185 pKa = 4.38KK186 pKa = 10.83PHH188 pKa = 6.6QPNDD192 pKa = 3.62PTNPLPPVGGFNPSPVNPSVPPSPVEE218 pKa = 3.9KK219 pKa = 10.15PDD221 pKa = 3.28VQEE224 pKa = 4.03PDD226 pKa = 3.1KK227 pKa = 11.5TEE229 pKa = 3.84TSDD232 pKa = 3.31TGVINAIKK240 pKa = 10.52NLNDD244 pKa = 4.59DD245 pKa = 4.33LNKK248 pKa = 10.79SNTDD252 pKa = 2.65IHH254 pKa = 8.08NDD256 pKa = 3.02MNNIFSTMNDD266 pKa = 3.02ALRR269 pKa = 11.84QLNSTNTAIGQSIVEE284 pKa = 4.13QMKK287 pKa = 10.24QDD289 pKa = 3.35AQIYY293 pKa = 9.46DD294 pKa = 3.74NKK296 pKa = 10.73KK297 pKa = 9.53LQQQIAANNINAINSQTKK315 pKa = 10.42SLLEE319 pKa = 4.1GNKK322 pKa = 10.26SITGSITGNTDD333 pKa = 3.13RR334 pKa = 11.84LVAAVNASGDD344 pKa = 4.06GVVSAIDD351 pKa = 3.62GLADD355 pKa = 3.9KK356 pKa = 11.15LKK358 pKa = 11.0LCDD361 pKa = 4.36PNTDD365 pKa = 3.45PFNCEE370 pKa = 3.87GEE372 pKa = 4.26NGLTPSSVEE381 pKa = 4.33SILKK385 pKa = 7.6QTSAVVTTSQVDD397 pKa = 3.44AEE399 pKa = 4.48EE400 pKa = 4.65GLLTTLKK407 pKa = 9.68EE408 pKa = 4.42TIDD411 pKa = 3.49NNLIEE416 pKa = 4.77DD417 pKa = 4.08TQSYY421 pKa = 9.8LEE423 pKa = 4.62DD424 pKa = 3.65MKK426 pKa = 11.49SDD428 pKa = 5.18LIGALPNSSQCDD440 pKa = 3.23VDD442 pKa = 4.21VLKK445 pKa = 10.08TPYY448 pKa = 10.58GDD450 pKa = 4.07FSIGCEE456 pKa = 3.59YY457 pKa = 10.67SARR460 pKa = 11.84LKK462 pKa = 10.96SILAFVFYY470 pKa = 10.25IYY472 pKa = 10.36TLYY475 pKa = 10.4TLAEE479 pKa = 3.96ILFTGVTPVAGTVPYY494 pKa = 10.16FSRR497 pKa = 11.84RR498 pKa = 3.44

Molecular weight: 53.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O80261|O80261_9VIRU Orf 498 OS=Vibrio phage fs2 OX=83201 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.33KK3 pKa = 8.43STKK6 pKa = 10.04AALIVASLAAVAATGANAAIPQEE29 pKa = 4.14AQAALDD35 pKa = 3.65AVGEE39 pKa = 4.33FTSTVVGWMWGVGTAMIVGFVGLKK63 pKa = 9.2VVKK66 pKa = 10.45KK67 pKa = 9.96GANKK71 pKa = 8.84ATT73 pKa = 3.64

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

2445

38

716

271.7

30.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.344 ± 1.502

1.472 ± 0.363

5.644 ± 0.797

5.031 ± 0.853

4.663 ± 0.576

5.808 ± 0.496

1.554 ± 0.616

6.748 ± 0.679

5.89 ± 0.571

9.243 ± 0.688

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.484

4.499 ± 0.712

4.54 ± 0.515

3.681 ± 0.342

4.663 ± 1.152

7.894 ± 0.816

6.83 ± 0.736

7.117 ± 0.904

1.35 ± 0.465

2.822 ± 0.403
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski