Amino acid dipepetide frequency for Clostridium sp. D5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.051AlaAla: 8.051 ± 0.086	1.192AlaCys: 1.192 ± 0.031	4.618AlaAsp: 4.618 ± 0.062	5.797AlaGlu: 5.797 ± 0.08	3.096AlaPhe: 3.096 ± 0.047	6.87AlaGly: 6.87 ± 0.079	1.11AlaHis: 1.11 ± 0.027	4.807AlaIle: 4.807 ± 0.067	4.461AlaLys: 4.461 ± 0.054	7.004AlaLeu: 7.004 ± 0.08	2.426AlaMet: 2.426 ± 0.042	2.264AlaAsn: 2.264 ± 0.04	2.127AlaPro: 2.127 ± 0.041	2.179AlaGln: 2.179 ± 0.044	2.99AlaArg: 2.99 ± 0.048	3.909AlaSer: 3.909 ± 0.058	2.801AlaThr: 2.801 ± 0.048	6.99AlaVal: 6.99 ± 0.082	0.717AlaTrp: 0.717 ± 0.023	2.634AlaTyr: 2.634 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.078CysAla: 1.078 ± 0.026	0.3CysCys: 0.3 ± 0.014	0.801CysAsp: 0.801 ± 0.025	0.989CysGlu: 0.989 ± 0.025	0.696CysPhe: 0.696 ± 0.02	1.566CysGly: 1.566 ± 0.033	0.291CysHis: 0.291 ± 0.013	1.197CysIle: 1.197 ± 0.031	0.713CysLys: 0.713 ± 0.023	1.226CysLeu: 1.226 ± 0.028	0.528CysMet: 0.528 ± 0.017	0.549CysAsn: 0.549 ± 0.021	0.695CysPro: 0.695 ± 0.027	0.433CysGln: 0.433 ± 0.019	0.914CysArg: 0.914 ± 0.026	0.983CysSer: 0.983 ± 0.029	0.797CysThr: 0.797 ± 0.024	1.021CysVal: 1.021 ± 0.028	0.128CysTrp: 0.128 ± 0.009	0.585CysTyr: 0.585 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.147AspAla: 4.147 ± 0.063	0.789AspCys: 0.789 ± 0.028	2.709AspAsp: 2.709 ± 0.051	4.353AspGlu: 4.353 ± 0.058	2.445AspPhe: 2.445 ± 0.038	4.409AspGly: 4.409 ± 0.062	0.801AspHis: 0.801 ± 0.024	4.579AspIle: 4.579 ± 0.055	3.41AspLys: 3.41 ± 0.046	4.32AspLeu: 4.32 ± 0.056	1.916AspMet: 1.916 ± 0.038	2.095AspAsn: 2.095 ± 0.048	1.783AspPro: 1.783 ± 0.037	1.349AspGln: 1.349 ± 0.029	2.325AspArg: 2.325 ± 0.04	3.118AspSer: 3.118 ± 0.046	3.158AspThr: 3.158 ± 0.049	3.787AspVal: 3.787 ± 0.059	0.582AspTrp: 0.582 ± 0.021	2.663AspTyr: 2.663 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.799GluAla: 5.799 ± 0.069	0.931GluCys: 0.931 ± 0.024	4.263GluAsp: 4.263 ± 0.054	7.495GluGlu: 7.495 ± 0.112	2.687GluPhe: 2.687 ± 0.043	4.769GluGly: 4.769 ± 0.065	1.372GluHis: 1.372 ± 0.028	5.549GluIle: 5.549 ± 0.062	6.351GluLys: 6.351 ± 0.068	6.902GluLeu: 6.902 ± 0.079	2.617GluMet: 2.617 ± 0.041	4.051GluAsn: 4.051 ± 0.061	2.056GluPro: 2.056 ± 0.043	2.892GluGln: 2.892 ± 0.044	3.316GluArg: 3.316 ± 0.055	3.472GluSer: 3.472 ± 0.053	3.696GluThr: 3.696 ± 0.052	4.627GluVal: 4.627 ± 0.057	0.722GluTrp: 0.722 ± 0.025	3.259GluTyr: 3.259 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	2.913PheAla: 2.913 ± 0.049	0.765PheCys: 0.765 ± 0.021	2.335PheAsp: 2.335 ± 0.039	2.643PheGlu: 2.643 ± 0.046	1.808PhePhe: 1.808 ± 0.047	3.187PheGly: 3.187 ± 0.052	0.877PheHis: 0.877 ± 0.022	2.913PheIle: 2.913 ± 0.05	1.966PheLys: 1.966 ± 0.041	3.982PheLeu: 3.982 ± 0.073	1.28PheMet: 1.28 ± 0.033	1.422PheAsn: 1.422 ± 0.034	1.463PhePro: 1.463 ± 0.032	1.431PheGln: 1.431 ± 0.03	1.743PheArg: 1.743 ± 0.034	2.883PheSer: 2.883 ± 0.044	2.401PheThr: 2.401 ± 0.036	2.707PheVal: 2.707 ± 0.044	0.494PheTrp: 0.494 ± 0.018	1.77PheTyr: 1.77 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	5.286GlyAla: 5.286 ± 0.063	1.378GlyCys: 1.378 ± 0.032	3.605GlyAsp: 3.605 ± 0.063	4.876GlyGlu: 4.876 ± 0.058	3.16GlyPhe: 3.16 ± 0.045	5.533GlyGly: 5.533 ± 0.084	1.328GlyHis: 1.328 ± 0.031	6.794GlyIle: 6.794 ± 0.077	5.3GlyLys: 5.3 ± 0.067	6.235GlyLeu: 6.235 ± 0.069	2.77GlyMet: 2.77 ± 0.045	3.15GlyAsn: 3.15 ± 0.058	1.467GlyPro: 1.467 ± 0.036	2.242GlyGln: 2.242 ± 0.043	3.461GlyArg: 3.461 ± 0.051	4.265GlySer: 4.265 ± 0.072	4.392GlyThr: 4.392 ± 0.069	5.092GlyVal: 5.092 ± 0.061	0.765GlyTrp: 0.765 ± 0.026	3.422GlyTyr: 3.422 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.108HisAla: 1.108 ± 0.031	0.309HisCys: 0.309 ± 0.013	0.866HisAsp: 0.866 ± 0.023	1.042HisGlu: 1.042 ± 0.029	0.845HisPhe: 0.845 ± 0.024	1.286HisGly: 1.286 ± 0.033	0.38HisHis: 0.38 ± 0.016	1.471HisIle: 1.471 ± 0.033	0.923HisLys: 0.923 ± 0.026	1.523HisLeu: 1.523 ± 0.033	0.602HisMet: 0.602 ± 0.021	0.708HisAsn: 0.708 ± 0.022	0.909HisPro: 0.909 ± 0.025	0.545HisGln: 0.545 ± 0.02	0.77HisArg: 0.77 ± 0.022	0.993HisSer: 0.993 ± 0.027	0.961HisThr: 0.961 ± 0.022	1.082HisVal: 1.082 ± 0.025	0.194HisTrp: 0.194 ± 0.012	0.759HisTyr: 0.759 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.66IleAla: 5.66 ± 0.062	1.364IleCys: 1.364 ± 0.031	3.799IleAsp: 3.799 ± 0.049	4.856IleGlu: 4.856 ± 0.064	3.023IlePhe: 3.023 ± 0.056	5.221IleGly: 5.221 ± 0.068	1.38IleHis: 1.38 ± 0.031	5.163IleIle: 5.163 ± 0.066	3.744IleLys: 3.744 ± 0.059	7.445IleLeu: 7.445 ± 0.093	2.216IleMet: 2.216 ± 0.037	2.705IleAsn: 2.705 ± 0.05	3.305IlePro: 3.305 ± 0.051	2.525IleGln: 2.525 ± 0.038	3.626IleArg: 3.626 ± 0.052	4.9IleSer: 4.9 ± 0.058	4.254IleThr: 4.254 ± 0.061	4.917IleVal: 4.917 ± 0.059	0.672IleTrp: 0.672 ± 0.022	2.835IleTyr: 2.835 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	4.955LysAla: 4.955 ± 0.064	0.699LysCys: 0.699 ± 0.024	3.645LysAsp: 3.645 ± 0.048	6.455LysGlu: 6.455 ± 0.073	1.779LysPhe: 1.779 ± 0.037	4.102LysGly: 4.102 ± 0.047	0.936LysHis: 0.936 ± 0.024	4.401LysIle: 4.401 ± 0.06	5.652LysLys: 5.652 ± 0.069	4.921LysLeu: 4.921 ± 0.056	2.192LysMet: 2.192 ± 0.039	3.287LysAsn: 3.287 ± 0.055	1.994LysPro: 1.994 ± 0.04	2.243LysGln: 2.243 ± 0.048	2.946LysArg: 2.946 ± 0.048	3.245LysSer: 3.245 ± 0.044	3.541LysThr: 3.541 ± 0.051	4.138LysVal: 4.138 ± 0.058	0.646LysTrp: 0.646 ± 0.022	2.758LysTyr: 2.758 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.792LeuAla: 6.792 ± 0.069	1.521LeuCys: 1.521 ± 0.034	5.084LeuAsp: 5.084 ± 0.059	6.713LeuGlu: 6.713 ± 0.074	3.824LeuPhe: 3.824 ± 0.065	6.321LeuGly: 6.321 ± 0.07	1.556LeuHis: 1.556 ± 0.033	6.131LeuIle: 6.131 ± 0.083	6.026LeuLys: 6.026 ± 0.061	8.704LeuLeu: 8.704 ± 0.116	2.746LeuMet: 2.746 ± 0.042	3.885LeuAsn: 3.885 ± 0.049	3.613LeuPro: 3.613 ± 0.051	2.917LeuGln: 2.917 ± 0.045	3.761LeuArg: 3.761 ± 0.055	6.058LeuSer: 6.058 ± 0.073	5.034LeuThr: 5.034 ± 0.061	5.405LeuVal: 5.405 ± 0.067	0.854LeuTrp: 0.854 ± 0.022	3.389LeuTyr: 3.389 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.511MetAla: 2.511 ± 0.046	0.398MetCys: 0.398 ± 0.015	1.969MetAsp: 1.969 ± 0.035	2.707MetGlu: 2.707 ± 0.049	1.117MetPhe: 1.117 ± 0.026	2.25MetGly: 2.25 ± 0.037	0.522MetHis: 0.522 ± 0.015	2.296MetIle: 2.296 ± 0.042	2.681MetLys: 2.681 ± 0.042	3.006MetLeu: 3.006 ± 0.051	1.065MetMet: 1.065 ± 0.029	1.592MetAsn: 1.592 ± 0.034	1.236MetPro: 1.236 ± 0.032	1.177MetGln: 1.177 ± 0.029	1.3MetArg: 1.3 ± 0.032	1.893MetSer: 1.893 ± 0.039	1.789MetThr: 1.789 ± 0.036	1.985MetVal: 1.985 ± 0.041	0.269MetTrp: 0.269 ± 0.013	1.012MetTyr: 1.012 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.926AsnAla: 2.926 ± 0.045	0.623AsnCys: 0.623 ± 0.02	1.914AsnAsp: 1.914 ± 0.042	2.68AsnGlu: 2.68 ± 0.049	1.507AsnPhe: 1.507 ± 0.029	3.46AsnGly: 3.46 ± 0.062	0.767AsnHis: 0.767 ± 0.024	3.273AsnIle: 3.273 ± 0.049	2.284AsnLys: 2.284 ± 0.043	3.651AsnLeu: 3.651 ± 0.048	1.436AsnMet: 1.436 ± 0.028	1.649AsnAsn: 1.649 ± 0.039	2.013AsnPro: 2.013 ± 0.047	1.485AsnGln: 1.485 ± 0.037	1.896AsnArg: 1.896 ± 0.031	2.271AsnSer: 2.271 ± 0.043	2.317AsnThr: 2.317 ± 0.042	2.763AsnVal: 2.763 ± 0.045	0.447AsnTrp: 0.447 ± 0.019	1.816AsnTyr: 1.816 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.703ProAla: 2.703 ± 0.053	0.49ProCys: 0.49 ± 0.022	2.522ProAsp: 2.522 ± 0.043	3.361ProGlu: 3.361 ± 0.049	1.567ProPhe: 1.567 ± 0.038	2.603ProGly: 2.603 ± 0.042	0.618ProHis: 0.618 ± 0.019	2.006ProIle: 2.006 ± 0.037	1.817ProLys: 1.817 ± 0.04	2.964ProLeu: 2.964 ± 0.046	0.936ProMet: 0.936 ± 0.027	1.248ProAsn: 1.248 ± 0.029	0.862ProPro: 0.862 ± 0.036	1.099ProGln: 1.099 ± 0.03	1.085ProArg: 1.085 ± 0.03	1.887ProSer: 1.887 ± 0.043	1.499ProThr: 1.499 ± 0.034	3.198ProVal: 3.198 ± 0.047	0.376ProTrp: 0.376 ± 0.014	1.522ProTyr: 1.522 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.49GlnAla: 2.49 ± 0.041	0.427GlnCys: 0.427 ± 0.015	1.665GlnAsp: 1.665 ± 0.035	2.771GlnGlu: 2.771 ± 0.048	1.234GlnPhe: 1.234 ± 0.034	2.157GlnGly: 2.157 ± 0.034	0.467GlnHis: 0.467 ± 0.019	2.578GlnIle: 2.578 ± 0.042	2.644GlnLys: 2.644 ± 0.046	2.706GlnLeu: 2.706 ± 0.043	1.245GlnMet: 1.245 ± 0.028	1.685GlnAsn: 1.685 ± 0.038	1.015GlnPro: 1.015 ± 0.028	1.158GlnGln: 1.158 ± 0.031	1.377GlnArg: 1.377 ± 0.036	1.688GlnSer: 1.688 ± 0.034	1.672GlnThr: 1.672 ± 0.036	2.09GlnVal: 2.09 ± 0.034	0.326GlnTrp: 0.326 ± 0.016	1.401GlnTyr: 1.401 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.818ArgAla: 2.818 ± 0.046	0.57ArgCys: 0.57 ± 0.019	2.168ArgAsp: 2.168 ± 0.039	3.996ArgGlu: 3.996 ± 0.053	1.918ArgPhe: 1.918 ± 0.035	2.599ArgGly: 2.599 ± 0.046	0.833ArgHis: 0.833 ± 0.026	3.516ArgIle: 3.516 ± 0.046	3.309ArgLys: 3.309 ± 0.05	4.074ArgLeu: 4.074 ± 0.061	1.609ArgMet: 1.609 ± 0.034	1.91ArgAsn: 1.91 ± 0.033	1.357ArgPro: 1.357 ± 0.038	1.784ArgGln: 1.784 ± 0.039	2.352ArgArg: 2.352 ± 0.044	2.087ArgSer: 2.087 ± 0.042	2.326ArgThr: 2.326 ± 0.044	2.638ArgVal: 2.638 ± 0.041	0.428ArgTrp: 0.428 ± 0.017	1.863ArgTyr: 1.863 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	4.309SerAla: 4.309 ± 0.057	0.886SerCys: 0.886 ± 0.026	3.082SerAsp: 3.082 ± 0.055	3.855SerGlu: 3.855 ± 0.057	2.638SerPhe: 2.638 ± 0.05	5.175SerGly: 5.175 ± 0.075	1.019SerHis: 1.019 ± 0.027	4.225SerIle: 4.225 ± 0.05	2.978SerLys: 2.978 ± 0.046	5.166SerLeu: 5.166 ± 0.062	1.888SerMet: 1.888 ± 0.039	1.997SerAsn: 1.997 ± 0.042	1.861SerPro: 1.861 ± 0.033	1.799SerGln: 1.799 ± 0.034	2.874SerArg: 2.874 ± 0.044	3.608SerSer: 3.608 ± 0.063	2.779SerThr: 2.779 ± 0.043	4.409SerVal: 4.409 ± 0.051	0.649SerTrp: 0.649 ± 0.028	2.371SerTyr: 2.371 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	4.704ThrAla: 4.704 ± 0.073	0.667ThrCys: 0.667 ± 0.02	3.133ThrAsp: 3.133 ± 0.055	3.892ThrGlu: 3.892 ± 0.051	2.179ThrPhe: 2.179 ± 0.039	4.791ThrGly: 4.791 ± 0.058	0.822ThrHis: 0.822 ± 0.023	3.744ThrIle: 3.744 ± 0.056	2.86ThrLys: 2.86 ± 0.05	4.772ThrLeu: 4.772 ± 0.054	1.474ThrMet: 1.474 ± 0.036	1.867ThrAsn: 1.867 ± 0.035	2.19ThrPro: 2.19 ± 0.044	1.46ThrGln: 1.46 ± 0.033	1.992ThrArg: 1.992 ± 0.037	2.848ThrSer: 2.848 ± 0.047	2.568ThrThr: 2.568 ± 0.044	4.45ThrVal: 4.45 ± 0.083	0.498ThrTrp: 0.498 ± 0.019	2.014ThrTyr: 2.014 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	4.365ValAla: 4.365 ± 0.061	1.309ValCys: 1.309 ± 0.034	3.56ValAsp: 3.56 ± 0.047	4.599ValGlu: 4.599 ± 0.06	3.209ValPhe: 3.209 ± 0.05	4.339ValGly: 4.339 ± 0.063	1.136ValHis: 1.136 ± 0.028	5.357ValIle: 5.357 ± 0.065	4.503ValLys: 4.503 ± 0.054	7.039ValLeu: 7.039 ± 0.071	2.21ValMet: 2.21 ± 0.041	2.855ValAsn: 2.855 ± 0.039	2.636ValPro: 2.636 ± 0.046	2.092ValGln: 2.092 ± 0.035	2.971ValArg: 2.971 ± 0.043	4.604ValSer: 4.604 ± 0.055	4.068ValThr: 4.068 ± 0.084	4.519ValVal: 4.519 ± 0.069	0.682ValTrp: 0.682 ± 0.023	2.754ValTyr: 2.754 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.636TrpAla: 0.636 ± 0.02	0.201TrpCys: 0.201 ± 0.013	0.64TrpAsp: 0.64 ± 0.02	0.715TrpGlu: 0.715 ± 0.024	0.414TrpPhe: 0.414 ± 0.018	0.816TrpGly: 0.816 ± 0.026	0.189TrpHis: 0.189 ± 0.013	0.666TrpIle: 0.666 ± 0.022	0.754TrpLys: 0.754 ± 0.021	0.952TrpLeu: 0.952 ± 0.028	0.376TrpMet: 0.376 ± 0.016	0.548TrpAsn: 0.548 ± 0.018	0.274TrpPro: 0.274 ± 0.014	0.382TrpGln: 0.382 ± 0.014	0.38TrpArg: 0.38 ± 0.019	0.517TrpSer: 0.517 ± 0.017	0.478TrpThr: 0.478 ± 0.021	0.532TrpVal: 0.532 ± 0.02	0.112TrpTrp: 0.112 ± 0.008	0.403TrpTyr: 0.403 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.79TyrAla: 2.79 ± 0.041	0.67TyrCys: 0.67 ± 0.022	2.362TyrAsp: 2.362 ± 0.044	3.033TyrGlu: 3.033 ± 0.049	1.836TyrPhe: 1.836 ± 0.039	3.068TyrGly: 3.068 ± 0.047	0.874TyrHis: 0.874 ± 0.022	2.945TyrIle: 2.945 ± 0.047	2.158TyrLys: 2.158 ± 0.045	3.741TyrLeu: 3.741 ± 0.05	1.241TyrMet: 1.241 ± 0.027	1.711TyrAsn: 1.711 ± 0.038	1.506TyrPro: 1.506 ± 0.033	1.601TyrGln: 1.601 ± 0.035	2.076TyrArg: 2.076 ± 0.04	2.342TyrSer: 2.342 ± 0.04	2.396TyrThr: 2.396 ± 0.041	2.458TyrVal: 2.458 ± 0.038	0.422TyrTrp: 0.422 ± 0.018	1.869TyrTyr: 1.869 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4496 proteins (1527431 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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