Metallosphaera yellowstonensis MK1

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Metallosphaera; Metallosphaera yellowstonensis

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2C5M8|H2C5M8_9CREN Rieske Fe-S protein OS=Metallosphaera yellowstonensis MK1 OX=671065 GN=MetMK1DRAFT_00018510 PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 10.55QNLLVGLLVLTLAVNVTLGYY22 pKa = 10.4EE23 pKa = 4.52LYY25 pKa = 10.45SLNHH29 pKa = 6.75ASSAHH34 pKa = 5.26AVAPVVFEE42 pKa = 4.12NVTVYY47 pKa = 10.51PGQNTTAEE55 pKa = 4.62GNLTLHH61 pKa = 6.74PFYY64 pKa = 10.99YY65 pKa = 10.36KK66 pKa = 10.79YY67 pKa = 10.97GVLLKK72 pKa = 10.21FPQKK76 pKa = 10.85GEE78 pKa = 3.98YY79 pKa = 10.13LVGINGTVFPGEE91 pKa = 4.42AILVQLEE98 pKa = 4.4DD99 pKa = 4.0GQSVLLNQNVTQGKK113 pKa = 10.16VIVNDD118 pKa = 3.67NVIHH122 pKa = 5.42ATIIISGLYY131 pKa = 9.73SGSAPSPQLVLQDD144 pKa = 3.27VGLFFQFLRR153 pKa = 11.84PLQVVNSSSEE163 pKa = 3.97GGQQEE168 pKa = 4.5IQNVTHH174 pKa = 7.24DD175 pKa = 4.07FDD177 pKa = 5.28HH178 pKa = 7.31GSVSDD183 pKa = 3.74NDD185 pKa = 4.27LNSSATNSTINSNTTINQDD204 pKa = 3.22NITNQNSTSQPHH216 pKa = 7.26DD217 pKa = 4.32DD218 pKa = 4.23NVTLQPHH225 pKa = 6.1NEE227 pKa = 3.91NDD229 pKa = 3.46TGQGTSQGSDD239 pKa = 2.51SSQNSNSSSNSSQNAHH255 pKa = 6.44EE256 pKa = 4.58SSSGDD261 pKa = 3.71SNSSDD266 pKa = 3.23SSGSGSSSSGSGSSSGSGSSSSGSGSSSSGQGDD299 pKa = 3.58SGDD302 pKa = 3.98SGSGSDD308 pKa = 3.57SSSGSGSQDD317 pKa = 2.74NN318 pKa = 3.75

Molecular weight: 32.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2C860|H2C860_9CREN 3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Metallosphaera yellowstonensis MK1 OX=671065 GN=MetMK1DRAFT_00027640 PE=3 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84NRR5 pKa = 11.84PSGLKK10 pKa = 9.86KK11 pKa = 10.31RR12 pKa = 11.84LSRR15 pKa = 11.84ALKK18 pKa = 10.54SNSAVPAWVILKK30 pKa = 9.31TNGKK34 pKa = 9.27FRR36 pKa = 11.84LNPRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84NDD48 pKa = 3.43LKK50 pKa = 11.39VV51 pKa = 2.95

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3340

0

3340

771653

21

1434

231.0

25.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.984 ± 0.034

0.666 ± 0.017

4.558 ± 0.035

7.15 ± 0.058

4.161 ± 0.038

7.453 ± 0.042

1.486 ± 0.018

6.466 ± 0.045

6.115 ± 0.055

10.959 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.019

3.576 ± 0.034

4.274 ± 0.035

2.265 ± 0.026

6.341 ± 0.06

6.982 ± 0.041

4.919 ± 0.038

9.013 ± 0.045

1.173 ± 0.017

3.946 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski