Bos indicus x Bos taurus (Hybrid cattle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria;

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4W2DTD4|A0A4W2DTD4_BOBOX Isoform of A0A4W2BYD9 Glutathione transferase OS=Bos indicus x Bos taurus OX=30522 GN=LOC113882304 PE=3 SV=1
MM1 pKa = 7.45GGDD4 pKa = 3.22AADD7 pKa = 4.69RR8 pKa = 11.84EE9 pKa = 4.39LGAAGGLGWGQTAFAPADD27 pKa = 3.35HH28 pKa = 6.86SSQEE32 pKa = 4.14KK33 pKa = 8.84ATEE36 pKa = 3.89NATDD40 pKa = 3.6RR41 pKa = 11.84LANGAQSIPHH51 pKa = 7.22DD52 pKa = 4.34SPAHH56 pKa = 5.9GEE58 pKa = 4.19GTHH61 pKa = 6.63CEE63 pKa = 4.09EE64 pKa = 4.67EE65 pKa = 4.83GFAEE69 pKa = 5.34DD70 pKa = 6.15DD71 pKa = 3.82EE72 pKa = 7.08DD73 pKa = 5.82SDD75 pKa = 5.27GEE77 pKa = 4.03PSPWEE82 pKa = 3.86LSEE85 pKa = 4.68GMSGCLPKK93 pKa = 10.57EE94 pKa = 3.68QAGDD98 pKa = 3.86LFHH101 pKa = 7.39EE102 pKa = 4.81DD103 pKa = 2.66WDD105 pKa = 4.61LEE107 pKa = 4.33LKK109 pKa = 10.49ADD111 pKa = 3.94QGNPYY116 pKa = 10.37DD117 pKa = 4.73ADD119 pKa = 5.11DD120 pKa = 3.93IQGCLSQEE128 pKa = 3.69VRR130 pKa = 11.84PWVCCAPQGDD140 pKa = 4.66MIYY143 pKa = 10.59DD144 pKa = 4.29PSWHH148 pKa = 6.9HH149 pKa = 6.75PPPLIPHH156 pKa = 5.6YY157 pKa = 10.76SKK159 pKa = 10.49MVFEE163 pKa = 5.05TGQFDD168 pKa = 4.21DD169 pKa = 6.01AEE171 pKa = 4.35DD172 pKa = 3.31

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4W2FF90|A0A4W2FF90_BOBOX Serine/threonine kinase 17b OS=Bos indicus x Bos taurus OX=30522 GN=STK17B PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21408

20743

42151

25731042

12

8798

610.4

67.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.067 ± 0.013

2.197 ± 0.011

4.835 ± 0.007

7.113 ± 0.015

3.584 ± 0.01

6.602 ± 0.016

2.54 ± 0.006

4.378 ± 0.012

5.773 ± 0.014

9.902 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.005

3.553 ± 0.009

6.333 ± 0.019

4.749 ± 0.012

5.801 ± 0.012

8.339 ± 0.015

5.294 ± 0.009

5.99 ± 0.009

1.239 ± 0.005

2.591 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski