Amino acid dipepetide frequency for Escherichia phage Stx2 II

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.833AlaAla: 7.833 ± 0.7
1.639AlaCys: 1.639 ± 0.339
4.117AlaAsp: 4.117 ± 0.466
6.266AlaGlu: 6.266 ± 0.608
3.716AlaPhe: 3.716 ± 0.417
7.068AlaGly: 7.068 ± 0.913
1.494AlaHis: 1.494 ± 0.213
4.335AlaIle: 4.335 ± 0.417
3.534AlaLys: 3.534 ± 0.436
7.687AlaLeu: 7.687 ± 0.629
2.805AlaMet: 2.805 ± 0.339
2.769AlaAsn: 2.769 ± 0.358
3.716AlaPro: 3.716 ± 0.358
4.19AlaGln: 4.19 ± 0.487
5.246AlaArg: 5.246 ± 0.475
6.412AlaSer: 6.412 ± 0.649
4.918AlaThr: 4.918 ± 0.58
5.975AlaVal: 5.975 ± 0.432
1.603AlaTrp: 1.603 ± 0.25
2.149AlaTyr: 2.149 ± 0.273
0.0AlaXaa: 0.0 ± 0.0
Cys
1.785CysAla: 1.785 ± 0.295
0.874CysCys: 0.874 ± 0.219
0.729CysAsp: 0.729 ± 0.167
0.874CysGlu: 0.874 ± 0.206
0.984CysPhe: 0.984 ± 0.228
1.312CysGly: 1.312 ± 0.272
1.093CysHis: 1.093 ± 0.237
1.057CysIle: 1.057 ± 0.227
0.619CysLys: 0.619 ± 0.2
1.494CysLeu: 1.494 ± 0.308
0.474CysMet: 0.474 ± 0.123
0.583CysAsn: 0.583 ± 0.172
1.129CysPro: 1.129 ± 0.224
0.692CysGln: 0.692 ± 0.169
2.04CysArg: 2.04 ± 0.425
2.259CysSer: 2.259 ± 0.385
1.057CysThr: 1.057 ± 0.243
1.494CysVal: 1.494 ± 0.273
0.401CysTrp: 0.401 ± 0.134
0.255CysTyr: 0.255 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
4.408AspAla: 4.408 ± 0.53
0.947AspCys: 0.947 ± 0.213
3.133AspAsp: 3.133 ± 0.303
3.242AspGlu: 3.242 ± 0.379
1.822AspPhe: 1.822 ± 0.249
3.497AspGly: 3.497 ± 0.39
0.801AspHis: 0.801 ± 0.206
2.987AspIle: 2.987 ± 0.367
3.17AspLys: 3.17 ± 0.297
3.206AspLeu: 3.206 ± 0.404
1.348AspMet: 1.348 ± 0.19
2.149AspAsn: 2.149 ± 0.323
1.822AspPro: 1.822 ± 0.331
1.312AspGln: 1.312 ± 0.281
2.623AspArg: 2.623 ± 0.357
3.024AspSer: 3.024 ± 0.323
2.259AspThr: 2.259 ± 0.314
3.097AspVal: 3.097 ± 0.346
0.911AspTrp: 0.911 ± 0.22
1.202AspTyr: 1.202 ± 0.257
0.0AspXaa: 0.0 ± 0.0
Glu
4.991GluAla: 4.991 ± 0.463
1.166GluCys: 1.166 ± 0.227
1.603GluAsp: 1.603 ± 0.263
2.987GluGlu: 2.987 ± 0.347
2.04GluPhe: 2.04 ± 0.313
2.805GluGly: 2.805 ± 0.472
1.057GluHis: 1.057 ± 0.185
3.024GluIle: 3.024 ± 0.385
3.607GluLys: 3.607 ± 0.419
4.7GluLeu: 4.7 ± 0.489
1.858GluMet: 1.858 ± 0.306
2.04GluAsn: 2.04 ± 0.349
1.931GluPro: 1.931 ± 0.315
3.279GluGln: 3.279 ± 0.397
4.226GluArg: 4.226 ± 0.54
3.279GluSer: 3.279 ± 0.355
2.732GluThr: 2.732 ± 0.564
3.425GluVal: 3.425 ± 0.474
0.729GluTrp: 0.729 ± 0.167
1.749GluTyr: 1.749 ± 0.248
0.0GluXaa: 0.0 ± 0.0
Phe
3.17PheAla: 3.17 ± 0.458
1.129PheCys: 1.129 ± 0.257
1.639PheAsp: 1.639 ± 0.236
1.093PheGlu: 1.093 ± 0.216
2.004PhePhe: 2.004 ± 0.385
2.113PheGly: 2.113 ± 0.25
1.348PheHis: 1.348 ± 0.187
2.477PheIle: 2.477 ± 0.38
1.421PheLys: 1.421 ± 0.231
3.097PheLeu: 3.097 ± 0.363
0.947PheMet: 0.947 ± 0.176
1.166PheAsn: 1.166 ± 0.239
2.295PhePro: 2.295 ± 0.357
1.384PheGln: 1.384 ± 0.201
3.534PheArg: 3.534 ± 0.392
4.226PheSer: 4.226 ± 0.443
2.878PheThr: 2.878 ± 0.325
2.404PheVal: 2.404 ± 0.297
0.838PheTrp: 0.838 ± 0.205
0.947PheTyr: 0.947 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
4.627GlyAla: 4.627 ± 0.744
1.421GlyCys: 1.421 ± 0.259
3.971GlyAsp: 3.971 ± 0.578
4.663GlyGlu: 4.663 ± 0.983
2.805GlyPhe: 2.805 ± 0.336
4.08GlyGly: 4.08 ± 0.595
1.057GlyHis: 1.057 ± 0.208
3.789GlyIle: 3.789 ± 0.456
3.643GlyLys: 3.643 ± 0.64
4.7GlyLeu: 4.7 ± 0.391
2.659GlyMet: 2.659 ± 0.329
2.914GlyAsn: 2.914 ± 0.321
3.388GlyPro: 3.388 ± 1.459
2.477GlyGln: 2.477 ± 0.328
3.752GlyArg: 3.752 ± 0.382
3.862GlySer: 3.862 ± 0.471
3.534GlyThr: 3.534 ± 0.408
4.627GlyVal: 4.627 ± 0.433
1.02GlyTrp: 1.02 ± 0.201
2.222GlyTyr: 2.222 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
1.676HisAla: 1.676 ± 0.218
0.911HisCys: 0.911 ± 0.184
0.947HisAsp: 0.947 ± 0.181
0.801HisGlu: 0.801 ± 0.171
1.129HisPhe: 1.129 ± 0.207
2.004HisGly: 2.004 ± 0.302
1.057HisHis: 1.057 ± 0.258
1.02HisIle: 1.02 ± 0.221
0.729HisLys: 0.729 ± 0.177
2.295HisLeu: 2.295 ± 0.39
0.437HisMet: 0.437 ± 0.129
0.874HisAsn: 0.874 ± 0.152
1.676HisPro: 1.676 ± 0.348
0.765HisGln: 0.765 ± 0.189
2.222HisArg: 2.222 ± 0.334
1.785HisSer: 1.785 ± 0.301
1.384HisThr: 1.384 ± 0.233
0.984HisVal: 0.984 ± 0.185
0.51HisTrp: 0.51 ± 0.152
0.474HisTyr: 0.474 ± 0.127
0.0HisXaa: 0.0 ± 0.0
Ile
4.044IleAla: 4.044 ± 0.358
1.239IleCys: 1.239 ± 0.234
3.06IleAsp: 3.06 ± 0.399
2.332IleGlu: 2.332 ± 0.341
1.931IlePhe: 1.931 ± 0.294
2.368IleGly: 2.368 ± 0.381
1.457IleHis: 1.457 ± 0.215
2.295IleIle: 2.295 ± 0.294
2.477IleLys: 2.477 ± 0.334
3.825IleLeu: 3.825 ± 0.518
1.02IleMet: 1.02 ± 0.198
2.587IleAsn: 2.587 ± 0.301
2.587IlePro: 2.587 ± 0.281
1.749IleGln: 1.749 ± 0.254
5.173IleArg: 5.173 ± 0.468
4.918IleSer: 4.918 ± 0.521
3.789IleThr: 3.789 ± 0.476
2.295IleVal: 2.295 ± 0.311
0.401IleTrp: 0.401 ± 0.126
1.093IleTyr: 1.093 ± 0.235
0.0IleXaa: 0.0 ± 0.0
Lys
4.809LysAla: 4.809 ± 0.398
0.437LysCys: 0.437 ± 0.132
2.404LysAsp: 2.404 ± 0.296
2.842LysGlu: 2.842 ± 0.36
0.765LysPhe: 0.765 ± 0.142
4.08LysGly: 4.08 ± 0.849
1.02LysHis: 1.02 ± 0.204
2.587LysIle: 2.587 ± 0.321
3.206LysLys: 3.206 ± 0.387
4.517LysLeu: 4.517 ± 0.406
1.494LysMet: 1.494 ± 0.223
2.914LysAsn: 2.914 ± 0.39
2.878LysPro: 2.878 ± 0.326
2.514LysGln: 2.514 ± 0.388
3.024LysArg: 3.024 ± 0.339
2.732LysSer: 2.732 ± 0.279
2.878LysThr: 2.878 ± 0.33
2.878LysVal: 2.878 ± 0.353
0.729LysTrp: 0.729 ± 0.186
1.457LysTyr: 1.457 ± 0.223
0.0LysXaa: 0.0 ± 0.0
Leu
7.359LeuAla: 7.359 ± 0.606
2.004LeuCys: 2.004 ± 0.341
2.914LeuAsp: 2.914 ± 0.329
3.133LeuGlu: 3.133 ± 0.38
3.68LeuPhe: 3.68 ± 0.46
3.352LeuGly: 3.352 ± 0.383
2.295LeuHis: 2.295 ± 0.311
4.117LeuIle: 4.117 ± 0.469
4.554LeuLys: 4.554 ± 0.422
8.197LeuLeu: 8.197 ± 0.692
2.477LeuMet: 2.477 ± 0.328
3.971LeuAsn: 3.971 ± 0.401
5.865LeuPro: 5.865 ± 0.686
3.315LeuGln: 3.315 ± 0.493
7.286LeuArg: 7.286 ± 0.708
7.177LeuSer: 7.177 ± 0.686
5.465LeuThr: 5.465 ± 0.475
4.918LeuVal: 4.918 ± 0.404
1.202LeuTrp: 1.202 ± 0.296
2.441LeuTyr: 2.441 ± 0.247
0.0LeuXaa: 0.0 ± 0.0
Met
2.805MetAla: 2.805 ± 0.28
0.255MetCys: 0.255 ± 0.105
1.166MetAsp: 1.166 ± 0.215
1.384MetGlu: 1.384 ± 0.21
0.984MetPhe: 0.984 ± 0.159
1.202MetGly: 1.202 ± 0.249
0.583MetHis: 0.583 ± 0.136
1.166MetIle: 1.166 ± 0.201
1.894MetLys: 1.894 ± 0.268
2.805MetLeu: 2.805 ± 0.342
0.911MetMet: 0.911 ± 0.175
1.312MetAsn: 1.312 ± 0.264
2.149MetPro: 2.149 ± 0.307
1.348MetGln: 1.348 ± 0.207
1.931MetArg: 1.931 ± 0.256
2.259MetSer: 2.259 ± 0.305
2.149MetThr: 2.149 ± 0.26
1.348MetVal: 1.348 ± 0.256
0.291MetTrp: 0.291 ± 0.102
0.692MetTyr: 0.692 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
3.971AsnAla: 3.971 ± 0.477
0.692AsnCys: 0.692 ± 0.167
1.894AsnAsp: 1.894 ± 0.252
2.186AsnGlu: 2.186 ± 0.257
1.348AsnPhe: 1.348 ± 0.206
2.623AsnGly: 2.623 ± 0.343
1.312AsnHis: 1.312 ± 0.246
2.441AsnIle: 2.441 ± 0.329
1.894AsnLys: 1.894 ± 0.262
2.842AsnLeu: 2.842 ± 0.356
0.874AsnMet: 0.874 ± 0.187
1.567AsnAsn: 1.567 ± 0.26
1.858AsnPro: 1.858 ± 0.278
1.603AsnGln: 1.603 ± 0.242
2.842AsnArg: 2.842 ± 0.334
2.842AsnSer: 2.842 ± 0.386
2.623AsnThr: 2.623 ± 0.327
1.858AsnVal: 1.858 ± 0.264
0.546AsnTrp: 0.546 ± 0.157
1.057AsnTyr: 1.057 ± 0.23
0.0AsnXaa: 0.0 ± 0.0
Pro
5.319ProAla: 5.319 ± 0.632
1.421ProCys: 1.421 ± 0.285
3.971ProAsp: 3.971 ± 0.401
4.59ProGlu: 4.59 ± 0.596
1.567ProPhe: 1.567 ± 0.231
3.315ProGly: 3.315 ± 0.423
1.129ProHis: 1.129 ± 0.239
1.384ProIle: 1.384 ± 0.284
2.222ProLys: 2.222 ± 0.507
4.517ProLeu: 4.517 ± 0.463
1.057ProMet: 1.057 ± 0.182
0.583ProAsn: 0.583 ± 0.132
2.404ProPro: 2.404 ± 0.353
2.477ProGln: 2.477 ± 0.441
2.696ProArg: 2.696 ± 0.311
3.825ProSer: 3.825 ± 0.475
2.004ProThr: 2.004 ± 0.27
5.683ProVal: 5.683 ± 0.647
0.619ProTrp: 0.619 ± 0.174
1.53ProTyr: 1.53 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
4.044GlnAla: 4.044 ± 0.543
0.947GlnCys: 0.947 ± 0.234
1.785GlnAsp: 1.785 ± 0.26
2.477GlnGlu: 2.477 ± 0.335
1.53GlnPhe: 1.53 ± 0.21
2.477GlnGly: 2.477 ± 0.526
1.202GlnHis: 1.202 ± 0.226
2.259GlnIle: 2.259 ± 0.349
2.878GlnLys: 2.878 ± 0.375
2.951GlnLeu: 2.951 ± 0.353
1.129GlnMet: 1.129 ± 0.18
1.822GlnAsn: 1.822 ± 0.228
2.623GlnPro: 2.623 ± 0.318
3.097GlnGln: 3.097 ± 0.565
3.752GlnArg: 3.752 ± 0.518
2.441GlnSer: 2.441 ± 0.286
1.931GlnThr: 1.931 ± 0.252
2.113GlnVal: 2.113 ± 0.357
0.765GlnTrp: 0.765 ± 0.186
1.093GlnTyr: 1.093 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
4.445ArgAla: 4.445 ± 0.322
1.348ArgCys: 1.348 ± 0.286
3.097ArgAsp: 3.097 ± 0.451
4.226ArgGlu: 4.226 ± 0.581
3.06ArgPhe: 3.06 ± 0.311
5.283ArgGly: 5.283 ± 0.717
2.368ArgHis: 2.368 ± 0.344
3.862ArgIle: 3.862 ± 0.429
4.627ArgLys: 4.627 ± 0.49
6.849ArgLeu: 6.849 ± 0.504
2.732ArgMet: 2.732 ± 0.333
3.097ArgAsn: 3.097 ± 0.331
3.097ArgPro: 3.097 ± 0.426
4.153ArgGln: 4.153 ± 0.425
6.995ArgArg: 6.995 ± 0.649
5.283ArgSer: 5.283 ± 0.473
3.898ArgThr: 3.898 ± 0.453
4.117ArgVal: 4.117 ± 0.417
1.239ArgTrp: 1.239 ± 0.202
2.186ArgTyr: 2.186 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
7.906SerAla: 7.906 ± 0.738
1.53SerCys: 1.53 ± 0.321
3.388SerAsp: 3.388 ± 0.34
3.097SerGlu: 3.097 ± 0.377
2.987SerPhe: 2.987 ± 0.342
6.849SerGly: 6.849 ± 0.538
1.093SerHis: 1.093 ± 0.183
3.133SerIle: 3.133 ± 0.365
2.222SerLys: 2.222 ± 0.295
7.141SerLeu: 7.141 ± 0.611
1.967SerMet: 1.967 ± 0.318
2.696SerAsn: 2.696 ± 0.363
4.517SerPro: 4.517 ± 0.48
2.878SerGln: 2.878 ± 0.312
6.23SerArg: 6.23 ± 0.542
6.157SerSer: 6.157 ± 1.096
3.789SerThr: 3.789 ± 0.415
5.173SerVal: 5.173 ± 0.53
0.911SerTrp: 0.911 ± 0.204
1.457SerTyr: 1.457 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
5.246ThrAla: 5.246 ± 0.432
0.874ThrCys: 0.874 ± 0.266
2.514ThrAsp: 2.514 ± 0.376
2.805ThrGlu: 2.805 ± 0.32
2.587ThrPhe: 2.587 ± 0.412
5.501ThrGly: 5.501 ± 0.573
1.384ThrHis: 1.384 ± 0.204
3.06ThrIle: 3.06 ± 0.4
2.732ThrLys: 2.732 ± 0.32
5.028ThrLeu: 5.028 ± 0.472
1.129ThrMet: 1.129 ± 0.183
1.384ThrAsn: 1.384 ± 0.2
3.497ThrPro: 3.497 ± 0.352
1.822ThrGln: 1.822 ± 0.267
2.951ThrArg: 2.951 ± 0.347
4.408ThrSer: 4.408 ± 0.521
3.388ThrThr: 3.388 ± 0.342
4.08ThrVal: 4.08 ± 0.419
0.911ThrTrp: 0.911 ± 0.174
1.202ThrTyr: 1.202 ± 0.233
0.0ThrXaa: 0.0 ± 0.0
Val
5.793ValAla: 5.793 ± 0.561
1.457ValCys: 1.457 ± 0.279
2.805ValAsp: 2.805 ± 0.331
2.514ValGlu: 2.514 ± 0.317
2.987ValPhe: 2.987 ± 0.385
2.732ValGly: 2.732 ± 0.332
1.057ValHis: 1.057 ± 0.192
3.68ValIle: 3.68 ± 0.384
2.805ValLys: 2.805 ± 0.326
6.339ValLeu: 6.339 ± 0.574
1.931ValMet: 1.931 ± 0.28
2.951ValAsn: 2.951 ± 0.312
2.914ValPro: 2.914 ± 0.337
2.04ValGln: 2.04 ± 0.314
5.465ValArg: 5.465 ± 0.788
5.355ValSer: 5.355 ± 0.478
4.007ValThr: 4.007 ± 0.429
4.445ValVal: 4.445 ± 0.405
1.166ValTrp: 1.166 ± 0.218
1.239ValTyr: 1.239 ± 0.212
0.0ValXaa: 0.0 ± 0.0
Trp
0.911TrpAla: 0.911 ± 0.218
0.219TrpCys: 0.219 ± 0.085
0.51TrpAsp: 0.51 ± 0.139
0.619TrpGlu: 0.619 ± 0.151
0.838TrpPhe: 0.838 ± 0.168
0.51TrpGly: 0.51 ± 0.123
0.291TrpHis: 0.291 ± 0.117
0.765TrpIle: 0.765 ± 0.178
0.947TrpLys: 0.947 ± 0.208
1.822TrpLeu: 1.822 ± 0.292
0.692TrpMet: 0.692 ± 0.151
0.583TrpAsn: 0.583 ± 0.147
0.546TrpPro: 0.546 ± 0.189
1.239TrpGln: 1.239 ± 0.191
1.457TrpArg: 1.457 ± 0.234
1.166TrpSer: 1.166 ± 0.196
0.583TrpThr: 0.583 ± 0.143
1.202TrpVal: 1.202 ± 0.22
0.364TrpTrp: 0.364 ± 0.13
0.364TrpTyr: 0.364 ± 0.107
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.324
0.692TyrCys: 0.692 ± 0.185
1.312TyrAsp: 1.312 ± 0.217
1.093TyrGlu: 1.093 ± 0.241
1.348TyrPhe: 1.348 ± 0.197
2.04TyrGly: 2.04 ± 0.359
0.583TyrHis: 0.583 ± 0.176
1.202TyrIle: 1.202 ± 0.255
0.984TyrLys: 0.984 ± 0.209
1.712TyrLeu: 1.712 ± 0.27
0.656TyrMet: 0.656 ± 0.137
0.911TyrAsn: 0.911 ± 0.192
1.421TyrPro: 1.421 ± 0.222
0.984TyrGln: 0.984 ± 0.196
2.514TyrArg: 2.514 ± 0.312
1.712TyrSer: 1.712 ± 0.296
1.275TyrThr: 1.275 ± 0.286
1.567TyrVal: 1.567 ± 0.257
0.474TyrTrp: 0.474 ± 0.134
0.765TyrTyr: 0.765 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 170 proteins (27450 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski