Mycoplasma gallinarum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162MHB5|A0A162MHB5_9MOLU Alanine--tRNA ligase OS=Mycoplasma gallinarum OX=29557 GN=alaS PE=3 SV=1
MM1 pKa = 6.96TFSVFSTVEE10 pKa = 3.83LFSDD14 pKa = 3.77EE15 pKa = 4.33EE16 pKa = 4.13LVEE19 pKa = 4.57FSVSSFPLFFGLSFTISSSEE39 pKa = 3.69WSFSSLLFSISLDD52 pKa = 3.94SEE54 pKa = 4.22WLSVSSLDD62 pKa = 3.95FGVKK66 pKa = 9.73SSEE69 pKa = 4.51TIFSLLWASSSIEE82 pKa = 4.04WSWLEE87 pKa = 4.0SFASLFKK94 pKa = 10.48WFSEE98 pKa = 4.25LFWDD102 pKa = 5.34DD103 pKa = 3.58VSDD106 pKa = 3.95SVNLFEE112 pKa = 5.9FSSFLLIEE120 pKa = 4.25SDD122 pKa = 3.32ALSFFSEE129 pKa = 4.59TPVTIFGSSEE139 pKa = 3.69LDD141 pKa = 3.29KK142 pKa = 11.38EE143 pKa = 4.55FTLSSFVSLLFIFLISPFWLKK164 pKa = 10.49QLGANKK170 pKa = 9.26TGQSKK175 pKa = 9.7PVNHH179 pKa = 6.84HH180 pKa = 5.96LCFFIFILMVV190 pKa = 3.56

Molecular weight: 21.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168RAD1|A0A168RAD1_9MOLU Lipoate--protein ligase OS=Mycoplasma gallinarum OX=29557 GN=lplA PE=4 SV=1
MM1 pKa = 7.67SLRR4 pKa = 11.84TYY6 pKa = 10.41QPNKK10 pKa = 8.14RR11 pKa = 11.84KK12 pKa = 9.28HH13 pKa = 5.69VKK15 pKa = 9.24VHH17 pKa = 5.39GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTANGRR29 pKa = 11.84KK30 pKa = 8.89VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.17GRR40 pKa = 11.84ARR42 pKa = 11.84LTVSDD47 pKa = 3.94KK48 pKa = 11.19RR49 pKa = 3.83

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

601

0

601

232620

37

6200

387.1

44.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.542 ± 0.104

0.39 ± 0.025

5.279 ± 0.103

7.04 ± 0.093

5.388 ± 0.134

4.398 ± 0.086

1.266 ± 0.041

9.407 ± 0.146

9.801 ± 0.092

10.058 ± 0.108

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.056

9.05 ± 0.186

2.649 ± 0.059

3.596 ± 0.079

2.757 ± 0.058

6.198 ± 0.071

5.179 ± 0.134

5.108 ± 0.067

0.99 ± 0.031

4.218 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski