Amino acid dipepetide frequency for Enterococcus phage vB_EfaP_IME199

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.521AlaAla: 2.521 ± 1.215
0.504AlaCys: 0.504 ± 0.374
4.202AlaAsp: 4.202 ± 1.009
5.043AlaGlu: 5.043 ± 0.981
3.026AlaPhe: 3.026 ± 0.815
3.866AlaGly: 3.866 ± 1.243
0.672AlaHis: 0.672 ± 0.27
3.362AlaIle: 3.362 ± 0.708
4.707AlaLys: 4.707 ± 0.585
5.715AlaLeu: 5.715 ± 0.895
1.513AlaMet: 1.513 ± 0.405
5.379AlaAsn: 5.379 ± 0.906
2.017AlaPro: 2.017 ± 0.566
2.017AlaGln: 2.017 ± 0.666
2.353AlaArg: 2.353 ± 0.595
3.026AlaSer: 3.026 ± 0.574
3.53AlaThr: 3.53 ± 0.78
2.858AlaVal: 2.858 ± 0.693
1.345AlaTrp: 1.345 ± 0.565
4.539AlaTyr: 4.539 ± 1.153
0.0AlaXaa: 0.0 ± 0.0
Cys
0.504CysAla: 0.504 ± 0.478
0.168CysCys: 0.168 ± 0.159
0.168CysAsp: 0.168 ± 0.156
0.168CysGlu: 0.168 ± 0.156
0.504CysPhe: 0.504 ± 0.264
0.336CysGly: 0.336 ± 0.313
0.0CysHis: 0.0 ± 0.0
0.672CysIle: 0.672 ± 0.395
0.336CysLys: 0.336 ± 0.222
0.168CysLeu: 0.168 ± 0.159
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.168CysPro: 0.168 ± 0.178
0.504CysGln: 0.504 ± 0.307
0.168CysArg: 0.168 ± 0.173
0.84CysSer: 0.84 ± 0.269
0.336CysThr: 0.336 ± 0.196
1.177CysVal: 1.177 ± 0.441
0.0CysTrp: 0.0 ± 0.0
0.336CysTyr: 0.336 ± 0.319
0.0CysXaa: 0.0 ± 0.0
Asp
3.026AspAla: 3.026 ± 0.843
0.168AspCys: 0.168 ± 0.173
2.69AspAsp: 2.69 ± 0.661
5.043AspGlu: 5.043 ± 0.893
3.866AspPhe: 3.866 ± 0.658
3.362AspGly: 3.362 ± 0.654
0.336AspHis: 0.336 ± 0.214
4.37AspIle: 4.37 ± 0.852
4.875AspLys: 4.875 ± 0.83
4.707AspLeu: 4.707 ± 1.286
1.177AspMet: 1.177 ± 0.462
4.875AspAsn: 4.875 ± 0.989
2.017AspPro: 2.017 ± 0.784
1.849AspGln: 1.849 ± 0.381
1.345AspArg: 1.345 ± 0.599
2.353AspSer: 2.353 ± 0.686
4.202AspThr: 4.202 ± 0.653
5.379AspVal: 5.379 ± 1.117
0.336AspTrp: 0.336 ± 0.203
3.194AspTyr: 3.194 ± 0.6
0.0AspXaa: 0.0 ± 0.0
Glu
3.026GluAla: 3.026 ± 0.684
0.168GluCys: 0.168 ± 0.156
3.698GluAsp: 3.698 ± 1.045
5.883GluGlu: 5.883 ± 1.304
3.866GluPhe: 3.866 ± 1.162
3.698GluGly: 3.698 ± 0.59
1.681GluHis: 1.681 ± 0.611
4.875GluIle: 4.875 ± 0.805
4.539GluLys: 4.539 ± 0.789
6.388GluLeu: 6.388 ± 1.357
1.849GluMet: 1.849 ± 0.391
5.043GluAsn: 5.043 ± 1.374
2.353GluPro: 2.353 ± 1.131
3.698GluGln: 3.698 ± 0.662
2.353GluArg: 2.353 ± 0.918
3.026GluSer: 3.026 ± 0.787
5.043GluThr: 5.043 ± 1.035
4.875GluVal: 4.875 ± 0.988
1.009GluTrp: 1.009 ± 0.408
3.362GluTyr: 3.362 ± 0.574
0.0GluXaa: 0.0 ± 0.0
Phe
2.858PheAla: 2.858 ± 0.663
0.672PheCys: 0.672 ± 0.387
3.698PheAsp: 3.698 ± 0.958
2.858PheGlu: 2.858 ± 0.703
1.513PhePhe: 1.513 ± 0.444
1.849PheGly: 1.849 ± 0.491
1.009PheHis: 1.009 ± 0.568
3.362PheIle: 3.362 ± 0.681
3.194PheLys: 3.194 ± 0.749
3.362PheLeu: 3.362 ± 1.115
1.345PheMet: 1.345 ± 0.444
3.362PheAsn: 3.362 ± 0.555
1.849PhePro: 1.849 ± 0.456
0.84PheGln: 0.84 ± 0.344
1.345PheArg: 1.345 ± 0.501
3.026PheSer: 3.026 ± 0.682
4.539PheThr: 4.539 ± 0.771
2.521PheVal: 2.521 ± 0.547
0.84PheTrp: 0.84 ± 0.392
2.353PheTyr: 2.353 ± 0.822
0.0PheXaa: 0.0 ± 0.0
Gly
3.194GlyAla: 3.194 ± 0.798
0.84GlyCys: 0.84 ± 0.375
2.858GlyAsp: 2.858 ± 0.68
3.362GlyGlu: 3.362 ± 0.892
3.53GlyPhe: 3.53 ± 0.872
4.539GlyGly: 4.539 ± 0.722
1.177GlyHis: 1.177 ± 0.587
3.362GlyIle: 3.362 ± 0.923
4.707GlyLys: 4.707 ± 1.378
4.707GlyLeu: 4.707 ± 0.769
2.353GlyMet: 2.353 ± 0.618
4.37GlyAsn: 4.37 ± 1.05
0.504GlyPro: 0.504 ± 0.277
2.353GlyGln: 2.353 ± 0.695
1.849GlyArg: 1.849 ± 0.544
5.043GlySer: 5.043 ± 1.612
3.698GlyThr: 3.698 ± 1.018
4.539GlyVal: 4.539 ± 0.772
0.336GlyTrp: 0.336 ± 0.245
2.858GlyTyr: 2.858 ± 0.519
0.0GlyXaa: 0.0 ± 0.0
His
1.009HisAla: 1.009 ± 0.444
0.168HisCys: 0.168 ± 0.156
0.672HisAsp: 0.672 ± 0.256
0.84HisGlu: 0.84 ± 0.336
1.681HisPhe: 1.681 ± 0.684
1.009HisGly: 1.009 ± 0.581
0.336HisHis: 0.336 ± 0.199
0.84HisIle: 0.84 ± 0.306
1.345HisLys: 1.345 ± 0.359
1.177HisLeu: 1.177 ± 0.528
0.504HisMet: 0.504 ± 0.241
1.345HisAsn: 1.345 ± 0.383
1.009HisPro: 1.009 ± 0.34
0.672HisGln: 0.672 ± 0.301
1.345HisArg: 1.345 ± 0.495
1.009HisSer: 1.009 ± 0.54
0.672HisThr: 0.672 ± 0.344
0.84HisVal: 0.84 ± 0.326
0.336HisTrp: 0.336 ± 0.223
1.513HisTyr: 1.513 ± 0.354
0.0HisXaa: 0.0 ± 0.0
Ile
4.707IleAla: 4.707 ± 0.817
0.336IleCys: 0.336 ± 0.319
5.883IleAsp: 5.883 ± 0.827
3.698IleGlu: 3.698 ± 0.965
1.513IlePhe: 1.513 ± 0.54
3.53IleGly: 3.53 ± 0.774
1.681IleHis: 1.681 ± 0.371
3.53IleIle: 3.53 ± 0.732
5.379IleLys: 5.379 ± 0.754
4.707IleLeu: 4.707 ± 0.655
1.345IleMet: 1.345 ± 0.37
4.034IleAsn: 4.034 ± 0.944
3.53IlePro: 3.53 ± 0.705
2.858IleGln: 2.858 ± 0.662
2.521IleArg: 2.521 ± 0.552
4.707IleSer: 4.707 ± 0.842
4.37IleThr: 4.37 ± 0.699
2.353IleVal: 2.353 ± 0.473
0.504IleTrp: 0.504 ± 0.371
3.194IleTyr: 3.194 ± 0.937
0.0IleXaa: 0.0 ± 0.0
Lys
4.539LysAla: 4.539 ± 0.985
0.504LysCys: 0.504 ± 0.276
3.866LysAsp: 3.866 ± 0.554
7.732LysGlu: 7.732 ± 0.879
3.698LysPhe: 3.698 ± 0.79
5.211LysGly: 5.211 ± 0.731
1.849LysHis: 1.849 ± 0.98
5.043LysIle: 5.043 ± 1.132
4.37LysLys: 4.37 ± 1.173
6.22LysLeu: 6.22 ± 1.032
1.513LysMet: 1.513 ± 0.541
3.53LysAsn: 3.53 ± 0.739
3.026LysPro: 3.026 ± 0.523
2.353LysGln: 2.353 ± 0.656
3.53LysArg: 3.53 ± 0.809
3.698LysSer: 3.698 ± 0.809
5.043LysThr: 5.043 ± 0.873
4.202LysVal: 4.202 ± 0.703
0.0LysTrp: 0.0 ± 0.0
4.202LysTyr: 4.202 ± 0.639
0.0LysXaa: 0.0 ± 0.0
Leu
5.211LeuAla: 5.211 ± 0.754
0.504LeuCys: 0.504 ± 0.248
4.034LeuAsp: 4.034 ± 0.986
5.547LeuGlu: 5.547 ± 1.057
2.69LeuPhe: 2.69 ± 0.542
6.051LeuGly: 6.051 ± 0.68
0.672LeuHis: 0.672 ± 0.246
4.202LeuIle: 4.202 ± 0.915
6.22LeuLys: 6.22 ± 1.166
4.539LeuLeu: 4.539 ± 0.795
1.849LeuMet: 1.849 ± 0.468
7.396LeuAsn: 7.396 ± 0.954
3.026LeuPro: 3.026 ± 0.82
4.034LeuGln: 4.034 ± 0.664
4.034LeuArg: 4.034 ± 0.838
5.043LeuSer: 5.043 ± 0.9
6.388LeuThr: 6.388 ± 0.927
3.362LeuVal: 3.362 ± 1.055
1.009LeuTrp: 1.009 ± 0.498
2.521LeuTyr: 2.521 ± 0.751
0.0LeuXaa: 0.0 ± 0.0
Met
2.353MetAla: 2.353 ± 0.485
0.168MetCys: 0.168 ± 0.16
1.009MetAsp: 1.009 ± 0.428
1.009MetGlu: 1.009 ± 0.443
1.177MetPhe: 1.177 ± 0.359
1.681MetGly: 1.681 ± 0.653
0.336MetHis: 0.336 ± 0.236
2.017MetIle: 2.017 ± 0.64
2.858MetLys: 2.858 ± 0.752
2.017MetLeu: 2.017 ± 0.686
0.336MetMet: 0.336 ± 0.227
3.698MetAsn: 3.698 ± 0.761
1.177MetPro: 1.177 ± 0.323
1.513MetGln: 1.513 ± 0.515
0.504MetArg: 0.504 ± 0.277
1.345MetSer: 1.345 ± 0.479
2.017MetThr: 2.017 ± 0.583
0.336MetVal: 0.336 ± 0.32
0.168MetTrp: 0.168 ± 0.141
0.672MetTyr: 0.672 ± 0.242
0.0MetXaa: 0.0 ± 0.0
Asn
5.379AsnAla: 5.379 ± 1.162
0.168AsnCys: 0.168 ± 0.159
3.362AsnAsp: 3.362 ± 0.695
4.875AsnGlu: 4.875 ± 0.803
2.858AsnPhe: 2.858 ± 0.743
5.379AsnGly: 5.379 ± 0.975
1.849AsnHis: 1.849 ± 0.458
5.379AsnIle: 5.379 ± 1.05
5.211AsnLys: 5.211 ± 0.872
6.724AsnLeu: 6.724 ± 0.995
1.849AsnMet: 1.849 ± 0.446
5.211AsnAsn: 5.211 ± 0.83
3.194AsnPro: 3.194 ± 0.624
2.353AsnGln: 2.353 ± 0.59
2.858AsnArg: 2.858 ± 0.652
4.37AsnSer: 4.37 ± 0.824
6.388AsnThr: 6.388 ± 0.925
2.353AsnVal: 2.353 ± 0.521
1.345AsnTrp: 1.345 ± 0.616
3.866AsnTyr: 3.866 ± 0.815
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.69
0.336ProCys: 0.336 ± 0.218
1.345ProAsp: 1.345 ± 0.559
2.69ProGlu: 2.69 ± 0.484
1.345ProPhe: 1.345 ± 0.508
1.513ProGly: 1.513 ± 0.568
0.672ProHis: 0.672 ± 0.315
2.185ProIle: 2.185 ± 0.598
2.858ProLys: 2.858 ± 0.504
2.185ProLeu: 2.185 ± 0.672
1.009ProMet: 1.009 ± 0.452
3.194ProAsn: 3.194 ± 0.721
1.345ProPro: 1.345 ± 0.763
2.185ProGln: 2.185 ± 1.194
0.504ProArg: 0.504 ± 0.261
3.194ProSer: 3.194 ± 0.643
2.521ProThr: 2.521 ± 0.531
1.849ProVal: 1.849 ± 0.593
0.336ProTrp: 0.336 ± 0.19
1.513ProTyr: 1.513 ± 0.409
0.0ProXaa: 0.0 ± 0.0
Gln
2.69GlnAla: 2.69 ± 0.55
0.672GlnCys: 0.672 ± 0.458
1.849GlnAsp: 1.849 ± 0.387
4.034GlnGlu: 4.034 ± 1.37
1.513GlnPhe: 1.513 ± 0.474
3.362GlnGly: 3.362 ± 0.689
1.345GlnHis: 1.345 ± 0.553
3.026GlnIle: 3.026 ± 0.665
2.017GlnLys: 2.017 ± 0.509
2.69GlnLeu: 2.69 ± 0.739
0.84GlnMet: 0.84 ± 0.434
2.858GlnAsn: 2.858 ± 0.62
0.672GlnPro: 0.672 ± 0.27
1.513GlnGln: 1.513 ± 0.555
1.681GlnArg: 1.681 ± 0.418
2.521GlnSer: 2.521 ± 0.714
2.521GlnThr: 2.521 ± 0.714
2.521GlnVal: 2.521 ± 0.557
0.84GlnTrp: 0.84 ± 0.355
1.513GlnTyr: 1.513 ± 0.394
0.0GlnXaa: 0.0 ± 0.0
Arg
2.521ArgAla: 2.521 ± 0.651
0.0ArgCys: 0.0 ± 0.0
1.849ArgAsp: 1.849 ± 0.448
2.017ArgGlu: 2.017 ± 0.422
2.185ArgPhe: 2.185 ± 0.779
1.681ArgGly: 1.681 ± 0.491
0.672ArgHis: 0.672 ± 0.394
1.849ArgIle: 1.849 ± 0.518
1.681ArgLys: 1.681 ± 0.473
3.194ArgLeu: 3.194 ± 0.84
1.177ArgMet: 1.177 ± 0.479
2.521ArgAsn: 2.521 ± 0.837
1.177ArgPro: 1.177 ± 0.388
2.017ArgGln: 2.017 ± 0.506
2.017ArgArg: 2.017 ± 0.627
2.185ArgSer: 2.185 ± 0.461
1.513ArgThr: 1.513 ± 0.416
2.185ArgVal: 2.185 ± 0.654
0.0ArgTrp: 0.0 ± 0.0
2.017ArgTyr: 2.017 ± 0.453
0.0ArgXaa: 0.0 ± 0.0
Ser
4.37SerAla: 4.37 ± 0.771
0.0SerCys: 0.0 ± 0.0
4.034SerAsp: 4.034 ± 0.686
3.362SerGlu: 3.362 ± 0.824
3.194SerPhe: 3.194 ± 0.496
4.034SerGly: 4.034 ± 1.23
1.177SerHis: 1.177 ± 0.307
4.707SerIle: 4.707 ± 0.612
5.379SerLys: 5.379 ± 0.926
4.202SerLeu: 4.202 ± 0.842
1.345SerMet: 1.345 ± 0.51
3.362SerAsn: 3.362 ± 0.822
1.681SerPro: 1.681 ± 0.402
2.185SerGln: 2.185 ± 0.596
1.345SerArg: 1.345 ± 0.43
4.034SerSer: 4.034 ± 0.691
3.866SerThr: 3.866 ± 0.773
2.017SerVal: 2.017 ± 0.586
1.177SerTrp: 1.177 ± 0.391
2.521SerTyr: 2.521 ± 0.564
0.0SerXaa: 0.0 ± 0.0
Thr
4.539ThrAla: 4.539 ± 0.867
0.504ThrCys: 0.504 ± 0.339
5.043ThrAsp: 5.043 ± 0.718
5.379ThrGlu: 5.379 ± 0.996
3.362ThrPhe: 3.362 ± 0.904
4.034ThrGly: 4.034 ± 0.958
0.672ThrHis: 0.672 ± 0.262
4.539ThrIle: 4.539 ± 0.96
4.875ThrLys: 4.875 ± 0.772
5.715ThrLeu: 5.715 ± 0.933
1.849ThrMet: 1.849 ± 0.733
4.539ThrAsn: 4.539 ± 0.967
3.194ThrPro: 3.194 ± 0.761
3.698ThrGln: 3.698 ± 0.89
1.009ThrArg: 1.009 ± 0.374
2.858ThrSer: 2.858 ± 0.536
6.724ThrThr: 6.724 ± 1.856
6.22ThrVal: 6.22 ± 0.801
0.84ThrTrp: 0.84 ± 0.272
2.858ThrTyr: 2.858 ± 0.75
0.0ThrXaa: 0.0 ± 0.0
Val
3.026ValAla: 3.026 ± 0.803
0.336ValCys: 0.336 ± 0.197
4.875ValAsp: 4.875 ± 0.894
3.362ValGlu: 3.362 ± 0.631
2.185ValPhe: 2.185 ± 0.444
2.185ValGly: 2.185 ± 0.413
0.84ValHis: 0.84 ± 0.415
4.034ValIle: 4.034 ± 0.858
5.547ValLys: 5.547 ± 0.99
4.034ValLeu: 4.034 ± 0.527
1.849ValMet: 1.849 ± 0.548
4.875ValAsn: 4.875 ± 0.763
1.681ValPro: 1.681 ± 0.641
2.185ValGln: 2.185 ± 0.544
1.849ValArg: 1.849 ± 0.625
3.362ValSer: 3.362 ± 0.662
4.539ValThr: 4.539 ± 0.675
2.858ValVal: 2.858 ± 0.458
0.672ValTrp: 0.672 ± 0.322
2.69ValTyr: 2.69 ± 0.587
0.0ValXaa: 0.0 ± 0.0
Trp
0.672TrpAla: 0.672 ± 0.285
0.168TrpCys: 0.168 ± 0.159
0.168TrpAsp: 0.168 ± 0.156
1.177TrpGlu: 1.177 ± 0.443
0.504TrpPhe: 0.504 ± 0.323
0.672TrpGly: 0.672 ± 0.519
0.336TrpHis: 0.336 ± 0.219
0.84TrpIle: 0.84 ± 0.256
1.009TrpLys: 1.009 ± 0.65
1.177TrpLeu: 1.177 ± 0.25
0.504TrpMet: 0.504 ± 0.273
0.672TrpAsn: 0.672 ± 0.298
0.0TrpPro: 0.0 ± 0.0
0.336TrpGln: 0.336 ± 0.345
0.336TrpArg: 0.336 ± 0.226
0.336TrpSer: 0.336 ± 0.319
1.681TrpThr: 1.681 ± 0.732
0.504TrpVal: 0.504 ± 0.318
0.0TrpTrp: 0.0 ± 0.0
0.672TrpTyr: 0.672 ± 0.309
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.866TyrAla: 3.866 ± 1.006
0.168TyrCys: 0.168 ± 0.156
3.866TyrAsp: 3.866 ± 0.811
2.521TyrGlu: 2.521 ± 1.197
2.185TyrPhe: 2.185 ± 0.518
2.185TyrGly: 2.185 ± 0.803
1.009TyrHis: 1.009 ± 0.401
2.185TyrIle: 2.185 ± 0.753
3.026TyrLys: 3.026 ± 0.573
4.707TyrLeu: 4.707 ± 0.506
2.017TyrMet: 2.017 ± 0.622
4.707TyrAsn: 4.707 ± 0.833
1.345TyrPro: 1.345 ± 0.57
1.513TyrGln: 1.513 ± 0.497
1.513TyrArg: 1.513 ± 0.432
2.017TyrSer: 2.017 ± 0.49
2.858TyrThr: 2.858 ± 0.634
4.034TyrVal: 4.034 ± 0.652
0.672TyrTrp: 0.672 ± 0.607
2.858TyrTyr: 2.858 ± 0.747
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 22 proteins (5950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski