Amino acid dipepetide frequency for Mycoplasma putrefaciens Mput9231

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.856AlaAla: 2.856 ± 0.154
0.512AlaCys: 0.512 ± 0.047
2.831AlaAsp: 2.831 ± 0.105
2.928AlaGlu: 2.928 ± 0.113
2.649AlaPhe: 2.649 ± 0.114
2.822AlaGly: 2.822 ± 0.141
0.817AlaHis: 0.817 ± 0.061
5.691AlaIle: 5.691 ± 0.196
6.266AlaLys: 6.266 ± 0.192
4.925AlaLeu: 4.925 ± 0.162
1.151AlaMet: 1.151 ± 0.08
3.757AlaAsn: 3.757 ± 0.118
1.134AlaPro: 1.134 ± 0.076
1.709AlaGln: 1.709 ± 0.097
1.976AlaArg: 1.976 ± 0.105
3.474AlaSer: 3.474 ± 0.124
2.915AlaThr: 2.915 ± 0.112
3.427AlaVal: 3.427 ± 0.126
0.554AlaTrp: 0.554 ± 0.058
1.917AlaTyr: 1.917 ± 0.099
0.0AlaXaa: 0.0 ± 0.0
Cys
0.271CysAla: 0.271 ± 0.035
0.055CysCys: 0.055 ± 0.014
0.364CysAsp: 0.364 ± 0.044
0.317CysGlu: 0.317 ± 0.047
0.385CysPhe: 0.385 ± 0.042
0.393CysGly: 0.393 ± 0.051
0.089CysHis: 0.089 ± 0.021
0.406CysIle: 0.406 ± 0.042
0.406CysLys: 0.406 ± 0.044
0.69CysLeu: 0.69 ± 0.056
0.106CysMet: 0.106 ± 0.019
0.33CysAsn: 0.33 ± 0.034
0.123CysPro: 0.123 ± 0.024
0.305CysGln: 0.305 ± 0.034
0.118CysArg: 0.118 ± 0.021
0.427CysSer: 0.427 ± 0.045
0.334CysThr: 0.334 ± 0.04
0.267CysVal: 0.267 ± 0.033
0.093CysTrp: 0.093 ± 0.02
0.216CysTyr: 0.216 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
2.915AspAla: 2.915 ± 0.112
0.258AspCys: 0.258 ± 0.036
3.199AspAsp: 3.199 ± 0.113
3.393AspGlu: 3.393 ± 0.145
3.677AspPhe: 3.677 ± 0.166
2.352AspGly: 2.352 ± 0.092
1.109AspHis: 1.109 ± 0.071
4.498AspIle: 4.498 ± 0.163
5.737AspLys: 5.737 ± 0.183
6.63AspLeu: 6.63 ± 0.195
0.956AspMet: 0.956 ± 0.075
3.571AspAsn: 3.571 ± 0.142
1.794AspPro: 1.794 ± 0.095
4.189AspGln: 4.189 ± 0.156
1.426AspArg: 1.426 ± 0.063
3.571AspSer: 3.571 ± 0.144
2.154AspThr: 2.154 ± 0.112
3.199AspVal: 3.199 ± 0.113
0.69AspTrp: 0.69 ± 0.052
2.784AspTyr: 2.784 ± 0.113
0.0AspXaa: 0.0 ± 0.0
Glu
2.797GluAla: 2.797 ± 0.122
0.254GluCys: 0.254 ± 0.03
2.297GluAsp: 2.297 ± 0.113
3.452GluGlu: 3.452 ± 0.16
3.368GluPhe: 3.368 ± 0.117
1.836GluGly: 1.836 ± 0.095
0.77GluHis: 0.77 ± 0.062
7.286GluIle: 7.286 ± 0.204
5.877GluLys: 5.877 ± 0.185
6.858GluLeu: 6.858 ± 0.211
1.396GluMet: 1.396 ± 0.084
4.151GluAsn: 4.151 ± 0.158
1.519GluPro: 1.519 ± 0.107
3.355GluGln: 3.355 ± 0.136
1.718GluArg: 1.718 ± 0.101
3.0GluSer: 3.0 ± 0.113
2.847GluThr: 2.847 ± 0.114
3.491GluVal: 3.491 ± 0.149
0.508GluTrp: 0.508 ± 0.049
2.204GluTyr: 2.204 ± 0.102
0.004GluXaa: 0.004 ± 0.004
Phe
2.957PheAla: 2.957 ± 0.137
0.389PheCys: 0.389 ± 0.044
3.821PheAsp: 3.821 ± 0.141
3.249PheGlu: 3.249 ± 0.118
2.361PhePhe: 2.361 ± 0.124
2.53PheGly: 2.53 ± 0.116
0.478PheHis: 0.478 ± 0.046
4.73PheIle: 4.73 ± 0.175
5.445PheLys: 5.445 ± 0.178
4.811PheLeu: 4.811 ± 0.162
0.812PheMet: 0.812 ± 0.058
4.328PheAsn: 4.328 ± 0.171
0.99PhePro: 0.99 ± 0.07
1.502PheGln: 1.502 ± 0.081
1.333PheArg: 1.333 ± 0.067
3.867PheSer: 3.867 ± 0.141
2.585PheThr: 2.585 ± 0.099
3.173PheVal: 3.173 ± 0.121
0.52PheTrp: 0.52 ± 0.05
1.87PheTyr: 1.87 ± 0.091
0.0PheXaa: 0.0 ± 0.0
Gly
2.869GlyAla: 2.869 ± 0.134
0.317GlyCys: 0.317 ± 0.041
2.154GlyAsp: 2.154 ± 0.107
2.297GlyGlu: 2.297 ± 0.113
2.699GlyPhe: 2.699 ± 0.119
2.53GlyGly: 2.53 ± 0.139
0.715GlyHis: 0.715 ± 0.063
4.544GlyIle: 4.544 ± 0.161
3.507GlyLys: 3.507 ± 0.137
4.713GlyLeu: 4.713 ± 0.15
1.142GlyMet: 1.142 ± 0.071
2.056GlyAsn: 2.056 ± 0.102
1.058GlyPro: 1.058 ± 0.065
1.743GlyGln: 1.743 ± 0.09
1.536GlyArg: 1.536 ± 0.088
2.953GlySer: 2.953 ± 0.108
2.407GlyThr: 2.407 ± 0.104
3.203GlyVal: 3.203 ± 0.132
0.567GlyTrp: 0.567 ± 0.047
2.128GlyTyr: 2.128 ± 0.104
0.0GlyXaa: 0.0 ± 0.0
His
0.825HisAla: 0.825 ± 0.065
0.118HisCys: 0.118 ± 0.023
0.664HisAsp: 0.664 ± 0.051
0.66HisGlu: 0.66 ± 0.05
0.762HisPhe: 0.762 ± 0.053
0.778HisGly: 0.778 ± 0.06
0.322HisHis: 0.322 ± 0.034
1.235HisIle: 1.235 ± 0.081
1.235HisLys: 1.235 ± 0.075
1.379HisLeu: 1.379 ± 0.075
0.216HisMet: 0.216 ± 0.031
1.041HisAsn: 1.041 ± 0.072
0.533HisPro: 0.533 ± 0.051
0.707HisGln: 0.707 ± 0.058
0.444HisArg: 0.444 ± 0.044
0.88HisSer: 0.88 ± 0.063
0.66HisThr: 0.66 ± 0.056
0.609HisVal: 0.609 ± 0.05
0.123HisTrp: 0.123 ± 0.025
0.453HisTyr: 0.453 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.708IleAla: 5.708 ± 0.181
0.778IleCys: 0.778 ± 0.067
6.131IleAsp: 6.131 ± 0.19
5.5IleGlu: 5.5 ± 0.167
4.671IlePhe: 4.671 ± 0.15
4.688IleGly: 4.688 ± 0.161
0.838IleHis: 0.838 ± 0.061
8.745IleIle: 8.745 ± 0.247
9.68IleLys: 9.68 ± 0.208
8.487IleLeu: 8.487 ± 0.2
1.392IleMet: 1.392 ± 0.078
7.337IleAsn: 7.337 ± 0.191
2.501IlePro: 2.501 ± 0.107
3.093IleGln: 3.093 ± 0.105
2.843IleArg: 2.843 ± 0.128
7.366IleSer: 7.366 ± 0.187
5.636IleThr: 5.636 ± 0.139
5.585IleVal: 5.585 ± 0.186
0.728IleTrp: 0.728 ± 0.054
3.326IleTyr: 3.326 ± 0.117
0.004IleXaa: 0.004 ± 0.005
Lys
5.115LysAla: 5.115 ± 0.135
0.288LysCys: 0.288 ± 0.04
6.105LysAsp: 6.105 ± 0.19
6.672LysGlu: 6.672 ± 0.212
4.261LysPhe: 4.261 ± 0.145
3.457LysGly: 3.457 ± 0.135
1.443LysHis: 1.443 ± 0.078
9.875LysIle: 9.875 ± 0.23
10.252LysLys: 10.252 ± 0.244
9.075LysLeu: 9.075 ± 0.253
2.2LysMet: 2.2 ± 0.103
8.25LysAsn: 8.25 ± 0.203
2.89LysPro: 2.89 ± 0.112
5.894LysGln: 5.894 ± 0.186
2.818LysArg: 2.818 ± 0.118
5.323LysSer: 5.323 ± 0.156
6.342LysThr: 6.342 ± 0.172
5.619LysVal: 5.619 ± 0.169
1.13LysTrp: 1.13 ± 0.071
4.388LysTyr: 4.388 ± 0.161
0.0LysXaa: 0.0 ± 0.0
Leu
5.805LeuAla: 5.805 ± 0.169
0.436LeuCys: 0.436 ± 0.043
6.638LeuAsp: 6.638 ± 0.184
7.163LeuGlu: 7.163 ± 0.214
5.005LeuPhe: 5.005 ± 0.193
4.404LeuGly: 4.404 ± 0.171
0.977LeuHis: 0.977 ± 0.062
9.439LeuIle: 9.439 ± 0.237
10.294LeuLys: 10.294 ± 0.25
8.805LeuLeu: 8.805 ± 0.24
1.654LeuMet: 1.654 ± 0.08
7.04LeuAsn: 7.04 ± 0.193
2.543LeuPro: 2.543 ± 0.107
2.924LeuGln: 2.924 ± 0.125
2.649LeuArg: 2.649 ± 0.12
6.545LeuSer: 6.545 ± 0.185
6.042LeuThr: 6.042 ± 0.173
6.025LeuVal: 6.025 ± 0.171
0.808LeuTrp: 0.808 ± 0.057
2.966LeuTyr: 2.966 ± 0.109
0.0LeuXaa: 0.0 ± 0.0
Met
1.045MetAla: 1.045 ± 0.082
0.097MetCys: 0.097 ± 0.019
0.8MetAsp: 0.8 ± 0.058
0.44MetGlu: 0.44 ± 0.042
1.32MetPhe: 1.32 ± 0.096
0.884MetGly: 0.884 ± 0.061
0.267MetHis: 0.267 ± 0.035
2.035MetIle: 2.035 ± 0.099
1.84MetLys: 1.84 ± 0.089
1.857MetLeu: 1.857 ± 0.093
0.343MetMet: 0.343 ± 0.038
1.299MetAsn: 1.299 ± 0.076
0.63MetPro: 0.63 ± 0.047
0.922MetGln: 0.922 ± 0.061
0.588MetArg: 0.588 ± 0.057
1.316MetSer: 1.316 ± 0.081
0.982MetThr: 0.982 ± 0.077
0.948MetVal: 0.948 ± 0.065
0.182MetTrp: 0.182 ± 0.025
0.567MetTyr: 0.567 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
3.122AsnAla: 3.122 ± 0.132
0.338AsnCys: 0.338 ± 0.037
4.184AsnAsp: 4.184 ± 0.147
3.207AsnGlu: 3.207 ± 0.135
3.905AsnPhe: 3.905 ± 0.164
2.746AsnGly: 2.746 ± 0.11
1.447AsnHis: 1.447 ± 0.087
6.067AsnIle: 6.067 ± 0.173
8.64AsnLys: 8.64 ± 0.226
7.248AsnLeu: 7.248 ± 0.202
1.1AsnMet: 1.1 ± 0.068
6.452AsnAsn: 6.452 ± 0.232
2.238AsnPro: 2.238 ± 0.105
4.675AsnGln: 4.675 ± 0.164
1.959AsnArg: 1.959 ± 0.096
4.828AsnSer: 4.828 ± 0.178
3.326AsnThr: 3.326 ± 0.123
3.351AsnVal: 3.351 ± 0.147
1.028AsnTrp: 1.028 ± 0.091
3.194AsnTyr: 3.194 ± 0.107
0.0AsnXaa: 0.0 ± 0.0
Pro
1.244ProAla: 1.244 ± 0.077
0.123ProCys: 0.123 ± 0.023
1.544ProAsp: 1.544 ± 0.114
1.95ProGlu: 1.95 ± 0.115
1.303ProPhe: 1.303 ± 0.074
1.468ProGly: 1.468 ± 0.082
0.368ProHis: 0.368 ± 0.041
2.56ProIle: 2.56 ± 0.114
2.365ProLys: 2.365 ± 0.117
2.306ProLeu: 2.306 ± 0.09
0.432ProMet: 0.432 ± 0.055
1.849ProAsn: 1.849 ± 0.094
0.381ProPro: 0.381 ± 0.061
0.783ProGln: 0.783 ± 0.077
0.77ProArg: 0.77 ± 0.071
1.862ProSer: 1.862 ± 0.098
1.819ProThr: 1.819 ± 0.116
1.578ProVal: 1.578 ± 0.087
0.228ProTrp: 0.228 ± 0.031
0.88ProTyr: 0.88 ± 0.061
0.0ProXaa: 0.0 ± 0.0
Gln
3.139GlnAla: 3.139 ± 0.12
0.08GlnCys: 0.08 ± 0.02
2.285GlnAsp: 2.285 ± 0.091
2.945GlnGlu: 2.945 ± 0.124
1.853GlnPhe: 1.853 ± 0.09
1.51GlnGly: 1.51 ± 0.09
0.613GlnHis: 0.613 ± 0.056
4.595GlnIle: 4.595 ± 0.16
4.726GlnLys: 4.726 ± 0.138
4.489GlnLeu: 4.489 ± 0.196
0.677GlnMet: 0.677 ± 0.053
3.546GlnAsn: 3.546 ± 0.118
1.104GlnPro: 1.104 ± 0.091
2.67GlnGln: 2.67 ± 0.119
1.324GlnArg: 1.324 ± 0.072
2.01GlnSer: 2.01 ± 0.097
2.911GlnThr: 2.911 ± 0.113
2.568GlnVal: 2.568 ± 0.109
0.41GlnTrp: 0.41 ± 0.04
1.464GlnTyr: 1.464 ± 0.08
0.0GlnXaa: 0.0 ± 0.0
Arg
1.519ArgAla: 1.519 ± 0.08
0.186ArgCys: 0.186 ± 0.033
1.489ArgAsp: 1.489 ± 0.077
1.887ArgGlu: 1.887 ± 0.101
1.667ArgPhe: 1.667 ± 0.085
1.312ArgGly: 1.312 ± 0.082
0.402ArgHis: 0.402 ± 0.043
2.653ArgIle: 2.653 ± 0.12
2.56ArgLys: 2.56 ± 0.11
2.721ArgLeu: 2.721 ± 0.106
0.893ArgMet: 0.893 ± 0.062
1.722ArgAsn: 1.722 ± 0.091
0.944ArgPro: 0.944 ± 0.061
1.345ArgGln: 1.345 ± 0.083
1.062ArgArg: 1.062 ± 0.069
1.895ArgSer: 1.895 ± 0.1
1.62ArgThr: 1.62 ± 0.088
1.735ArgVal: 1.735 ± 0.106
0.377ArgTrp: 0.377 ± 0.044
1.252ArgTyr: 1.252 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
3.321SerAla: 3.321 ± 0.105
0.415SerCys: 0.415 ± 0.042
3.634SerAsp: 3.634 ± 0.153
3.935SerGlu: 3.935 ± 0.135
3.52SerPhe: 3.52 ± 0.127
3.33SerGly: 3.33 ± 0.11
0.787SerHis: 0.787 ± 0.056
5.636SerIle: 5.636 ± 0.161
6.82SerLys: 6.82 ± 0.186
6.96SerLeu: 6.96 ± 0.188
1.214SerMet: 1.214 ± 0.08
4.726SerAsn: 4.726 ± 0.208
1.388SerPro: 1.388 ± 0.092
2.505SerGln: 2.505 ± 0.09
1.912SerArg: 1.912 ± 0.083
4.976SerSer: 4.976 ± 0.185
3.613SerThr: 3.613 ± 0.144
3.558SerVal: 3.558 ± 0.119
0.842SerTrp: 0.842 ± 0.065
2.598SerTyr: 2.598 ± 0.121
0.0SerXaa: 0.0 ± 0.0
Thr
2.691ThrAla: 2.691 ± 0.116
0.224ThrCys: 0.224 ± 0.031
3.287ThrAsp: 3.287 ± 0.125
2.602ThrGlu: 2.602 ± 0.1
2.704ThrPhe: 2.704 ± 0.121
3.084ThrGly: 3.084 ± 0.111
0.762ThrHis: 0.762 ± 0.056
5.289ThrIle: 5.289 ± 0.138
5.585ThrLys: 5.585 ± 0.182
4.781ThrLeu: 4.781 ± 0.149
0.863ThrMet: 0.863 ± 0.064
4.633ThrAsn: 4.633 ± 0.163
1.705ThrPro: 1.705 ± 0.107
1.684ThrGln: 1.684 ± 0.081
1.709ThrArg: 1.709 ± 0.081
4.261ThrSer: 4.261 ± 0.168
3.169ThrThr: 3.169 ± 0.118
3.093ThrVal: 3.093 ± 0.123
0.41ThrTrp: 0.41 ± 0.038
1.722ThrTyr: 1.722 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
3.414ValAla: 3.414 ± 0.142
0.482ValCys: 0.482 ± 0.047
3.436ValAsp: 3.436 ± 0.121
3.96ValGlu: 3.96 ± 0.164
3.114ValPhe: 3.114 ± 0.134
3.017ValGly: 3.017 ± 0.115
0.647ValHis: 0.647 ± 0.057
5.636ValIle: 5.636 ± 0.181
5.479ValLys: 5.479 ± 0.171
5.945ValLeu: 5.945 ± 0.173
1.011ValMet: 1.011 ± 0.075
3.52ValAsn: 3.52 ± 0.109
1.371ValPro: 1.371 ± 0.091
1.739ValGln: 1.739 ± 0.071
1.659ValArg: 1.659 ± 0.09
4.125ValSer: 4.125 ± 0.127
2.636ValThr: 2.636 ± 0.12
4.32ValVal: 4.32 ± 0.148
0.499ValTrp: 0.499 ± 0.049
2.128ValTyr: 2.128 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.465TrpAla: 0.465 ± 0.041
0.042TrpCys: 0.042 ± 0.013
0.783TrpAsp: 0.783 ± 0.103
0.639TrpGlu: 0.639 ± 0.052
0.512TrpPhe: 0.512 ± 0.046
0.368TrpGly: 0.368 ± 0.041
0.114TrpHis: 0.114 ± 0.02
1.092TrpIle: 1.092 ± 0.075
0.834TrpLys: 0.834 ± 0.058
1.011TrpLeu: 1.011 ± 0.066
0.216TrpMet: 0.216 ± 0.027
0.986TrpAsn: 0.986 ± 0.087
0.186TrpPro: 0.186 ± 0.03
0.347TrpGln: 0.347 ± 0.038
0.283TrpArg: 0.283 ± 0.036
0.668TrpSer: 0.668 ± 0.048
0.567TrpThr: 0.567 ± 0.053
0.499TrpVal: 0.499 ± 0.046
0.127TrpTrp: 0.127 ± 0.023
0.453TrpTyr: 0.453 ± 0.05
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.23TyrAla: 2.23 ± 0.101
0.3TyrCys: 0.3 ± 0.035
2.454TyrAsp: 2.454 ± 0.117
1.79TyrGlu: 1.79 ± 0.095
2.018TyrPhe: 2.018 ± 0.098
1.625TyrGly: 1.625 ± 0.084
0.681TyrHis: 0.681 ± 0.054
3.025TyrIle: 3.025 ± 0.11
3.846TyrLys: 3.846 ± 0.134
4.472TyrLeu: 4.472 ± 0.162
0.584TyrMet: 0.584 ± 0.063
2.462TyrAsn: 2.462 ± 0.118
0.783TyrPro: 0.783 ± 0.053
2.831TyrGln: 2.831 ± 0.128
1.113TyrArg: 1.113 ± 0.066
2.348TyrSer: 2.348 ± 0.098
1.692TyrThr: 1.692 ± 0.085
1.891TyrVal: 1.891 ± 0.097
0.368TyrTrp: 0.368 ± 0.037
1.582TyrTyr: 1.582 ± 0.074
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.004XaaGly: 0.004 ± 0.004
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.004XaaTyr: 0.004 ± 0.005
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 666 proteins (236353 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski