Amino acid dipepetide frequency for Methylobacterium symbioticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.612AlaAla: 22.612 ± 0.177	1.428AlaCys: 1.428 ± 0.03	7.212AlaAsp: 7.212 ± 0.062	9.368AlaGlu: 9.368 ± 0.086	4.736AlaPhe: 4.736 ± 0.053	13.088AlaGly: 13.088 ± 0.12	2.476AlaHis: 2.476 ± 0.044	5.881AlaIle: 5.881 ± 0.071	3.513AlaLys: 3.513 ± 0.06	16.492AlaLeu: 16.492 ± 0.122	3.48AlaMet: 3.48 ± 0.043	2.489AlaAsn: 2.489 ± 0.038	7.116AlaPro: 7.116 ± 0.078	4.397AlaGln: 4.397 ± 0.065	11.87AlaArg: 11.87 ± 0.108	6.533AlaSer: 6.533 ± 0.066	6.485AlaThr: 6.485 ± 0.078	10.638AlaVal: 10.638 ± 0.093	1.804AlaTrp: 1.804 ± 0.039	2.853AlaTyr: 2.853 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.131CysAla: 1.131 ± 0.027	0.112CysCys: 0.112 ± 0.009	0.483CysAsp: 0.483 ± 0.016	0.422CysGlu: 0.422 ± 0.017	0.289CysPhe: 0.289 ± 0.014	0.963CysGly: 0.963 ± 0.024	0.211CysHis: 0.211 ± 0.013	0.323CysIle: 0.323 ± 0.014	0.14CysLys: 0.14 ± 0.009	0.974CysLeu: 0.974 ± 0.025	0.146CysMet: 0.146 ± 0.01	0.166CysAsn: 0.166 ± 0.01	0.489CysPro: 0.489 ± 0.02	0.205CysGln: 0.205 ± 0.01	0.763CysArg: 0.763 ± 0.023	0.36CysSer: 0.36 ± 0.015	0.44CysThr: 0.44 ± 0.017	0.564CysVal: 0.564 ± 0.018	0.105CysTrp: 0.105 ± 0.008	0.163CysTyr: 0.163 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.602AspAla: 7.602 ± 0.078	0.416AspCys: 0.416 ± 0.016	2.621AspAsp: 2.621 ± 0.046	2.953AspGlu: 2.953 ± 0.046	1.807AspPhe: 1.807 ± 0.033	5.465AspGly: 5.465 ± 0.072	1.151AspHis: 1.151 ± 0.029	2.205AspIle: 2.205 ± 0.033	1.131AspLys: 1.131 ± 0.03	6.705AspLeu: 6.705 ± 0.071	0.924AspMet: 0.924 ± 0.023	0.835AspAsn: 0.835 ± 0.027	4.147AspPro: 4.147 ± 0.055	1.439AspGln: 1.439 ± 0.035	4.8AspArg: 4.8 ± 0.05	1.764AspSer: 1.764 ± 0.037	2.317AspThr: 2.317 ± 0.043	3.702AspVal: 3.702 ± 0.048	0.821AspTrp: 0.821 ± 0.024	1.222AspTyr: 1.222 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	9.696GluAla: 9.696 ± 0.095	0.296GluCys: 0.296 ± 0.014	2.466GluAsp: 2.466 ± 0.04	2.685GluGlu: 2.685 ± 0.049	1.323GluPhe: 1.323 ± 0.028	4.613GluGly: 4.613 ± 0.049	1.093GluHis: 1.093 ± 0.031	3.137GluIle: 3.137 ± 0.044	1.45GluLys: 1.45 ± 0.033	4.251GluLeu: 4.251 ± 0.059	1.238GluMet: 1.238 ± 0.03	1.106GluAsn: 1.106 ± 0.024	3.391GluPro: 3.391 ± 0.05	1.717GluGln: 1.717 ± 0.033	5.866GluArg: 5.866 ± 0.071	2.269GluSer: 2.269 ± 0.039	3.58GluThr: 3.58 ± 0.049	3.732GluVal: 3.732 ± 0.058	0.505GluTrp: 0.505 ± 0.017	0.743GluTyr: 0.743 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	4.609PheAla: 4.609 ± 0.054	0.359PheCys: 0.359 ± 0.015	2.161PheAsp: 2.161 ± 0.038	1.88PheGlu: 1.88 ± 0.04	1.16PhePhe: 1.16 ± 0.032	3.603PheGly: 3.603 ± 0.05	0.663PheHis: 0.663 ± 0.02	1.151PheIle: 1.151 ± 0.031	0.759PheLys: 0.759 ± 0.022	3.266PheLeu: 3.266 ± 0.049	0.596PheMet: 0.596 ± 0.02	0.773PheAsn: 0.773 ± 0.023	1.51PhePro: 1.51 ± 0.026	0.851PheGln: 0.851 ± 0.024	2.365PheArg: 2.365 ± 0.042	1.799PheSer: 1.799 ± 0.037	1.877PheThr: 1.877 ± 0.042	2.694PheVal: 2.694 ± 0.042	0.447PheTrp: 0.447 ± 0.02	0.773PheTyr: 0.773 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	11.254GlyAla: 11.254 ± 0.111	0.878GlyCys: 0.878 ± 0.023	4.216GlyAsp: 4.216 ± 0.059	4.677GlyGlu: 4.677 ± 0.059	3.601GlyPhe: 3.601 ± 0.055	8.11GlyGly: 8.11 ± 0.127	1.971GlyHis: 1.971 ± 0.034	4.357GlyIle: 4.357 ± 0.056	2.314GlyLys: 2.314 ± 0.045	11.111GlyLeu: 11.111 ± 0.104	1.973GlyMet: 1.973 ± 0.036	1.789GlyAsn: 1.789 ± 0.039	4.71GlyPro: 4.71 ± 0.064	2.991GlyGln: 2.991 ± 0.05	8.173GlyArg: 8.173 ± 0.089	4.942GlySer: 4.942 ± 0.063	5.217GlyThr: 5.217 ± 0.092	6.108GlyVal: 6.108 ± 0.059	1.383GlyTrp: 1.383 ± 0.028	2.315GlyTyr: 2.315 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.63HisAla: 2.63 ± 0.045	0.201HisCys: 0.201 ± 0.01	1.164HisAsp: 1.164 ± 0.028	0.93HisGlu: 0.93 ± 0.024	0.684HisPhe: 0.684 ± 0.02	2.085HisGly: 2.085 ± 0.042	0.579HisHis: 0.579 ± 0.024	0.68HisIle: 0.68 ± 0.02	0.346HisLys: 0.346 ± 0.017	2.219HisLeu: 2.219 ± 0.036	0.367HisMet: 0.367 ± 0.015	0.349HisAsn: 0.349 ± 0.013	1.416HisPro: 1.416 ± 0.031	0.508HisGln: 0.508 ± 0.019	1.664HisArg: 1.664 ± 0.038	0.68HisSer: 0.68 ± 0.023	0.731HisThr: 0.731 ± 0.021	1.442HisVal: 1.442 ± 0.031	0.315HisTrp: 0.315 ± 0.014	0.447HisTyr: 0.447 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.707IleAla: 6.707 ± 0.073	0.424IleCys: 0.424 ± 0.016	2.891IleAsp: 2.891 ± 0.04	3.012IleGlu: 3.012 ± 0.044	1.187IlePhe: 1.187 ± 0.028	4.612IleGly: 4.612 ± 0.06	0.769IleHis: 0.769 ± 0.024	1.303IleIle: 1.303 ± 0.031	0.976IleLys: 0.976 ± 0.028	4.258IleLeu: 4.258 ± 0.056	0.603IleMet: 0.603 ± 0.019	0.981IleAsn: 0.981 ± 0.025	2.177IlePro: 2.177 ± 0.038	1.111IleGln: 1.111 ± 0.027	3.393IleArg: 3.393 ± 0.048	1.964IleSer: 1.964 ± 0.04	2.125IleThr: 2.125 ± 0.041	3.685IleVal: 3.685 ± 0.054	0.457IleTrp: 0.457 ± 0.016	0.854IleTyr: 0.854 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	3.666LysAla: 3.666 ± 0.054	0.109LysCys: 0.109 ± 0.009	1.289LysAsp: 1.289 ± 0.031	1.102LysGlu: 1.102 ± 0.028	0.588LysPhe: 0.588 ± 0.019	2.18LysGly: 2.18 ± 0.043	0.392LysHis: 0.392 ± 0.015	1.131LysIle: 1.131 ± 0.031	0.728LysLys: 0.728 ± 0.029	2.35LysLeu: 2.35 ± 0.044	0.453LysMet: 0.453 ± 0.019	0.552LysAsn: 0.552 ± 0.02	1.873LysPro: 1.873 ± 0.039	0.69LysGln: 0.69 ± 0.022	1.941LysArg: 1.941 ± 0.039	1.178LysSer: 1.178 ± 0.027	1.394LysThr: 1.394 ± 0.03	1.923LysVal: 1.923 ± 0.035	0.218LysTrp: 0.218 ± 0.013	0.395LysTyr: 0.395 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	16.888LeuAla: 16.888 ± 0.13	0.981LeuCys: 0.981 ± 0.024	6.592LeuAsp: 6.592 ± 0.072	4.774LeuGlu: 4.774 ± 0.053	3.395LeuPhe: 3.395 ± 0.051	9.621LeuGly: 9.621 ± 0.098	1.917LeuHis: 1.917 ± 0.034	4.532LeuIle: 4.532 ± 0.059	2.91LeuLys: 2.91 ± 0.043	9.74LeuLeu: 9.74 ± 0.097	2.072LeuMet: 2.072 ± 0.035	2.22LeuAsn: 2.22 ± 0.041	6.195LeuPro: 6.195 ± 0.069	2.589LeuGln: 2.589 ± 0.043	8.338LeuArg: 8.338 ± 0.078	5.902LeuSer: 5.902 ± 0.063	5.87LeuThr: 5.87 ± 0.062	8.52LeuVal: 8.52 ± 0.081	1.125LeuTrp: 1.125 ± 0.028	2.033LeuTyr: 2.033 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.76MetAla: 2.76 ± 0.045	0.109MetCys: 0.109 ± 0.008	0.86MetAsp: 0.86 ± 0.024	0.874MetGlu: 0.874 ± 0.025	0.474MetPhe: 0.474 ± 0.017	1.498MetGly: 1.498 ± 0.03	0.367MetHis: 0.367 ± 0.014	1.034MetIle: 1.034 ± 0.029	0.612MetLys: 0.612 ± 0.019	2.117MetLeu: 2.117 ± 0.032	0.491MetMet: 0.491 ± 0.019	0.554MetAsn: 0.554 ± 0.019	1.58MetPro: 1.58 ± 0.034	0.682MetGln: 0.682 ± 0.02	1.899MetArg: 1.899 ± 0.037	1.4MetSer: 1.4 ± 0.029	1.463MetThr: 1.463 ± 0.028	1.316MetVal: 1.316 ± 0.029	0.136MetTrp: 0.136 ± 0.009	0.215MetTyr: 0.215 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.805AsnAla: 2.805 ± 0.041	0.172AsnCys: 0.172 ± 0.012	1.006AsnAsp: 1.006 ± 0.027	0.885AsnGlu: 0.885 ± 0.022	0.666AsnPhe: 0.666 ± 0.02	1.943AsnGly: 1.943 ± 0.04	0.387AsnHis: 0.387 ± 0.016	0.874AsnIle: 0.874 ± 0.023	0.469AsnLys: 0.469 ± 0.02	2.3AsnLeu: 2.3 ± 0.037	0.396AsnMet: 0.396 ± 0.017	0.512AsnAsn: 0.512 ± 0.022	1.69AsnPro: 1.69 ± 0.043	0.575AsnGln: 0.575 ± 0.02	1.686AsnArg: 1.686 ± 0.035	0.8AsnSer: 0.8 ± 0.024	1.014AsnThr: 1.014 ± 0.028	1.598AsnVal: 1.598 ± 0.036	0.272AsnTrp: 0.272 ± 0.013	0.464AsnTyr: 0.464 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.379ProAla: 8.379 ± 0.084	0.411ProCys: 0.411 ± 0.017	4.14ProAsp: 4.14 ± 0.06	4.4ProGlu: 4.4 ± 0.067	2.058ProPhe: 2.058 ± 0.035	5.735ProGly: 5.735 ± 0.07	1.111ProHis: 1.111 ± 0.028	2.11ProIle: 2.11 ± 0.038	1.48ProLys: 1.48 ± 0.032	5.224ProLeu: 5.224 ± 0.061	1.162ProMet: 1.162 ± 0.026	1.251ProAsn: 1.251 ± 0.03	3.32ProPro: 3.32 ± 0.061	1.59ProGln: 1.59 ± 0.031	3.934ProArg: 3.934 ± 0.055	2.794ProSer: 2.794 ± 0.042	2.64ProThr: 2.64 ± 0.053	4.884ProVal: 4.884 ± 0.063	0.718ProTrp: 0.718 ± 0.023	1.126ProTyr: 1.126 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.712GlnAla: 4.712 ± 0.068	0.185GlnCys: 0.185 ± 0.01	1.461GlnAsp: 1.461 ± 0.03	1.263GlnGlu: 1.263 ± 0.026	0.844GlnPhe: 0.844 ± 0.023	2.589GlnGly: 2.589 ± 0.046	0.519GlnHis: 0.519 ± 0.019	1.597GlnIle: 1.597 ± 0.035	0.749GlnLys: 0.749 ± 0.022	2.264GlnLeu: 2.264 ± 0.04	0.592GlnMet: 0.592 ± 0.019	0.657GlnAsn: 0.657 ± 0.026	1.711GlnPro: 1.711 ± 0.041	0.946GlnGln: 0.946 ± 0.031	2.302GlnArg: 2.302 ± 0.041	1.455GlnSer: 1.455 ± 0.038	1.561GlnThr: 1.561 ± 0.035	2.35GlnVal: 2.35 ± 0.04	0.277GlnTrp: 0.277 ± 0.014	0.483GlnTyr: 0.483 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	11.066ArgAla: 11.066 ± 0.089	0.569ArgCys: 0.569 ± 0.02	4.642ArgAsp: 4.642 ± 0.062	4.478ArgGlu: 4.478 ± 0.057	3.103ArgPhe: 3.103 ± 0.043	5.996ArgGly: 5.996 ± 0.074	1.957ArgHis: 1.957 ± 0.034	4.296ArgIle: 4.296 ± 0.046	1.798ArgLys: 1.798 ± 0.04	9.643ArgLeu: 9.643 ± 0.082	1.881ArgMet: 1.881 ± 0.035	1.728ArgAsn: 1.728 ± 0.034	5.0ArgPro: 5.0 ± 0.066	2.493ArgGln: 2.493 ± 0.04	7.567ArgArg: 7.567 ± 0.092	4.067ArgSer: 4.067 ± 0.056	4.13ArgThr: 4.13 ± 0.052	5.677ArgVal: 5.677 ± 0.062	1.029ArgTrp: 1.029 ± 0.024	1.73ArgTyr: 1.73 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.365SerAla: 6.365 ± 0.064	0.38SerCys: 0.38 ± 0.017	2.576SerAsp: 2.576 ± 0.041	2.579SerGlu: 2.579 ± 0.039	1.912SerPhe: 1.912 ± 0.036	5.371SerGly: 5.371 ± 0.062	0.925SerHis: 0.925 ± 0.022	1.99SerIle: 1.99 ± 0.041	1.074SerLys: 1.074 ± 0.028	5.37SerLeu: 5.37 ± 0.058	0.942SerMet: 0.942 ± 0.026	0.981SerAsn: 0.981 ± 0.025	2.734SerPro: 2.734 ± 0.043	1.317SerGln: 1.317 ± 0.032	3.579SerArg: 3.579 ± 0.052	2.125SerSer: 2.125 ± 0.041	2.371SerThr: 2.371 ± 0.045	3.747SerVal: 3.747 ± 0.057	0.61SerTrp: 0.61 ± 0.021	1.147SerTyr: 1.147 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	6.786ThrAla: 6.786 ± 0.112	0.442ThrCys: 0.442 ± 0.017	2.601ThrAsp: 2.601 ± 0.042	2.629ThrGlu: 2.629 ± 0.042	1.833ThrPhe: 1.833 ± 0.033	5.478ThrGly: 5.478 ± 0.071	0.906ThrHis: 0.906 ± 0.025	2.392ThrIle: 2.392 ± 0.056	1.166ThrLys: 1.166 ± 0.027	6.055ThrLeu: 6.055 ± 0.06	0.947ThrMet: 0.947 ± 0.026	1.086ThrAsn: 1.086 ± 0.03	3.262ThrPro: 3.262 ± 0.059	1.358ThrGln: 1.358 ± 0.033	3.692ThrArg: 3.692 ± 0.045	2.33ThrSer: 2.33 ± 0.041	2.578ThrThr: 2.578 ± 0.055	4.749ThrVal: 4.749 ± 0.073	0.673ThrTrp: 0.673 ± 0.018	1.163ThrTyr: 1.163 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.746ValAla: 10.746 ± 0.097	0.642ValCys: 0.642 ± 0.021	3.803ValAsp: 3.803 ± 0.051	4.719ValGlu: 4.719 ± 0.055	2.436ValPhe: 2.436 ± 0.04	6.11ValGly: 6.11 ± 0.073	1.371ValHis: 1.371 ± 0.028	3.305ValIle: 3.305 ± 0.044	1.785ValLys: 1.785 ± 0.035	8.086ValLeu: 8.086 ± 0.086	1.587ValMet: 1.587 ± 0.029	1.653ValAsn: 1.653 ± 0.032	4.458ValPro: 4.458 ± 0.048	2.107ValGln: 2.107 ± 0.037	5.842ValArg: 5.842 ± 0.072	4.126ValSer: 4.126 ± 0.056	4.588ValThr: 4.588 ± 0.062	6.343ValVal: 6.343 ± 0.082	0.819ValTrp: 0.819 ± 0.021	1.319ValTyr: 1.319 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.26TrpAla: 1.26 ± 0.025	0.141TrpCys: 0.141 ± 0.009	0.577TrpAsp: 0.577 ± 0.017	0.454TrpGlu: 0.454 ± 0.017	0.454TrpPhe: 0.454 ± 0.016	0.825TrpGly: 0.825 ± 0.023	0.288TrpHis: 0.288 ± 0.013	0.597TrpIle: 0.597 ± 0.02	0.319TrpLys: 0.319 ± 0.014	1.598TrpLeu: 1.598 ± 0.033	0.266TrpMet: 0.266 ± 0.012	0.333TrpAsn: 0.333 ± 0.015	0.677TrpPro: 0.677 ± 0.021	0.425TrpGln: 0.425 ± 0.018	1.274TrpArg: 1.274 ± 0.027	0.791TrpSer: 0.791 ± 0.022	0.772TrpThr: 0.772 ± 0.022	0.673TrpVal: 0.673 ± 0.023	0.201TrpTrp: 0.201 ± 0.011	0.264TrpTyr: 0.264 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.799TyrAla: 2.799 ± 0.038	0.192TyrCys: 0.192 ± 0.011	1.267TyrAsp: 1.267 ± 0.031	1.074TyrGlu: 1.074 ± 0.027	0.745TyrPhe: 0.745 ± 0.019	2.207TyrGly: 2.207 ± 0.045	0.385TyrHis: 0.385 ± 0.016	0.596TyrIle: 0.596 ± 0.023	0.432TyrLys: 0.432 ± 0.018	2.191TyrLeu: 2.191 ± 0.041	0.325TyrMet: 0.325 ± 0.014	0.499TyrAsn: 0.499 ± 0.02	1.01TyrPro: 1.01 ± 0.022	0.525TyrGln: 0.525 ± 0.018	1.903TyrArg: 1.903 ± 0.037	0.816TyrSer: 0.816 ± 0.024	0.974TyrThr: 0.974 ± 0.026	1.498TyrVal: 1.498 ± 0.033	0.273TyrTrp: 0.273 ± 0.013	0.468TyrTyr: 0.468 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5664 proteins (1690294 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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