Halarchaeum acidiphilum MH1-52-1

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halarchaeum; Halarchaeum acidiphilum

Average proteome isoelectric point is 5.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2706 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3A402|U3A402_9EURY 50S ribosomal protein L12 OS=Halarchaeum acidiphilum MH1-52-1 OX=1261545 GN=rpl12 PE=3 SV=1
MM1 pKa = 7.85SDD3 pKa = 3.83PEE5 pKa = 4.29TPATDD10 pKa = 3.52GGADD14 pKa = 3.63ADD16 pKa = 4.13GGADD20 pKa = 4.79NDD22 pKa = 4.21TDD24 pKa = 5.68DD25 pKa = 4.6YY26 pKa = 11.82GCPKK30 pKa = 10.09CGHH33 pKa = 6.72EE34 pKa = 4.51DD35 pKa = 3.27ATTGSISTTGSGLSKK50 pKa = 10.49MFDD53 pKa = 3.22VQTNNFEE60 pKa = 4.43TVTCEE65 pKa = 3.55NCGYY69 pKa = 9.03TEE71 pKa = 4.91LYY73 pKa = 9.94RR74 pKa = 11.84DD75 pKa = 3.41VRR77 pKa = 11.84NRR79 pKa = 11.84GSDD82 pKa = 3.48VVDD85 pKa = 3.39VFFGG89 pKa = 3.4

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3AER9|U3AER9_9EURY DUF1648 domain-containing protein OS=Halarchaeum acidiphilum MH1-52-1 OX=1261545 GN=MBEHAL_2033 PE=4 SV=1
MM1 pKa = 7.74ANPRR5 pKa = 11.84SRR7 pKa = 11.84PPPHH11 pKa = 6.62SRR13 pKa = 11.84PRR15 pKa = 11.84PPLHH19 pKa = 5.9PRR21 pKa = 11.84PRR23 pKa = 11.84RR24 pKa = 11.84LRR26 pKa = 11.84ARR28 pKa = 11.84DD29 pKa = 3.11RR30 pKa = 11.84ARR32 pKa = 11.84RR33 pKa = 11.84AVRR36 pKa = 11.84GG37 pKa = 3.58

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2706

0

2706

728013

37

1677

269.0

29.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.129 ± 0.082

0.778 ± 0.015

8.586 ± 0.062

8.105 ± 0.072

3.141 ± 0.028

8.579 ± 0.053

2.111 ± 0.024

3.503 ± 0.034

1.726 ± 0.023

8.854 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.68 ± 0.019

2.16 ± 0.028

4.583 ± 0.034

2.026 ± 0.03

7.317 ± 0.07

5.213 ± 0.045

6.278 ± 0.047

9.468 ± 0.058

1.047 ± 0.018

2.717 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski