Naematelia encephala

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Naemateliaceae; Naematelia

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2BGU1|A0A1Y2BGU1_9TREE Chromo domain-containing protein OS=Naematelia encephala OX=71784 GN=BCR39DRAFT_586415 PE=4 SV=1
MM1 pKa = 8.05ASTNPDD7 pKa = 3.23NDD9 pKa = 3.24HH10 pKa = 6.84HH11 pKa = 7.05SRR13 pKa = 11.84SSTHH17 pKa = 6.38QFWCDD22 pKa = 2.99LGALVDD28 pKa = 4.78SICSSGHH35 pKa = 6.73DD36 pKa = 4.12DD37 pKa = 4.55SMHH40 pKa = 5.95SADD43 pKa = 5.69GIGEE47 pKa = 4.28TARR50 pKa = 11.84TANLEE55 pKa = 3.99AEE57 pKa = 4.54GNGDD61 pKa = 3.43NDD63 pKa = 4.99GYY65 pKa = 10.91ATDD68 pKa = 3.8ADD70 pKa = 4.54EE71 pKa = 5.87RR72 pKa = 11.84PEE74 pKa = 5.7DD75 pKa = 5.11DD76 pKa = 4.74DD77 pKa = 5.9DD78 pKa = 5.63GYY80 pKa = 11.64YY81 pKa = 10.22AAKK84 pKa = 10.05ALHH87 pKa = 6.49SMDD90 pKa = 3.66TATHH94 pKa = 6.16NSADD98 pKa = 3.7GVTDD102 pKa = 3.54MANGDD107 pKa = 3.84AGMEE111 pKa = 4.0TAAVDD116 pKa = 4.14GADD119 pKa = 3.74NDD121 pKa = 4.37ASVQTVTEE129 pKa = 4.15TWDD132 pKa = 3.34TGPMEE137 pKa = 4.49TTTVTMVSGVPLNFCVTVALL157 pKa = 4.5

Molecular weight: 16.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2AKF3|A0A1Y2AKF3_9TREE PIPK domain-containing protein OS=Naematelia encephala OX=71784 GN=BCR39DRAFT_550308 PE=4 SV=1
MM1 pKa = 7.42QGIKK5 pKa = 10.39DD6 pKa = 3.72IGLQAQQARR15 pKa = 11.84WRR17 pKa = 11.84RR18 pKa = 11.84WFKK21 pKa = 9.9AQAWRR26 pKa = 11.84MANGVCRR33 pKa = 11.84QHH35 pKa = 6.89WISAICVVTVEE46 pKa = 4.84GYY48 pKa = 9.83VAALVDD54 pKa = 3.52WRR56 pKa = 11.84SQLNRR61 pKa = 11.84GEE63 pKa = 4.02NAYY66 pKa = 10.37ASSRR70 pKa = 11.84SSLMEE75 pKa = 4.35INHH78 pKa = 6.27KK79 pKa = 10.46CPSKK83 pKa = 10.78AYY85 pKa = 10.12GGAQVTMLTGEE96 pKa = 4.43HH97 pKa = 6.82PGEE100 pKa = 4.49SAQSHH105 pKa = 5.77WPPDD109 pKa = 3.18VFRR112 pKa = 11.84FLRR115 pKa = 11.84SDD117 pKa = 3.38NQRR120 pKa = 11.84KK121 pKa = 9.11LKK123 pKa = 10.87LSDD126 pKa = 3.77LSSTASSSEE135 pKa = 3.93GPTLCPVIVLALHH148 pKa = 6.5RR149 pKa = 11.84AKK151 pKa = 10.62ASHH154 pKa = 6.05EE155 pKa = 4.05KK156 pKa = 10.13RR157 pKa = 11.84RR158 pKa = 11.84PACVPSIGPSAVPFCSALEE177 pKa = 3.91WRR179 pKa = 11.84DD180 pKa = 3.33FFPRR184 pKa = 11.84AVKK187 pKa = 10.05GAPQNPAEE195 pKa = 4.24VLPGAGTLRR204 pKa = 11.84LMHH207 pKa = 6.93AEE209 pKa = 3.91EE210 pKa = 4.2LVVRR214 pKa = 11.84FLVPNEE220 pKa = 3.77RR221 pKa = 11.84RR222 pKa = 11.84PCGVGFPGKK231 pKa = 8.78STASAYY237 pKa = 8.9STGPGRR243 pKa = 11.84PSLAAPAPVAIPLAPRR259 pKa = 11.84SLPRR263 pKa = 11.84RR264 pKa = 11.84RR265 pKa = 11.84IARR268 pKa = 11.84EE269 pKa = 3.69PKK271 pKa = 9.93RR272 pKa = 11.84PPGSNPEE279 pKa = 4.03RR280 pKa = 11.84GADD283 pKa = 3.47RR284 pKa = 11.84QSPTNHH290 pKa = 6.77LASS293 pKa = 3.86

Molecular weight: 31.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7963

0

7963

3808585

51

4971

478.3

52.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.544 ± 0.026

1.056 ± 0.009

5.603 ± 0.019

6.137 ± 0.028

3.418 ± 0.017

7.198 ± 0.024

2.348 ± 0.012

4.806 ± 0.019

4.506 ± 0.024

9.179 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.009

3.14 ± 0.013

6.644 ± 0.036

3.738 ± 0.02

6.291 ± 0.026

8.976 ± 0.034

6.114 ± 0.017

6.236 ± 0.019

1.448 ± 0.011

2.501 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski