Amino acid dipepetide frequency for Lactococcus phage LW81

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.083AlaAla: 0.083 ± 0.044
0.165AlaCys: 0.165 ± 0.061
2.916AlaAsp: 2.916 ± 0.295
3.218AlaGlu: 3.218 ± 0.361
1.678AlaPhe: 1.678 ± 0.218
3.466AlaGly: 3.466 ± 0.667
0.688AlaHis: 0.688 ± 0.11
3.741AlaIle: 3.741 ± 0.395
5.034AlaLys: 5.034 ± 0.474
4.593AlaLeu: 4.593 ± 0.498
1.54AlaMet: 1.54 ± 0.276
3.026AlaAsn: 3.026 ± 0.255
1.045AlaPro: 1.045 ± 0.192
2.531AlaGln: 2.531 ± 0.302
1.87AlaArg: 1.87 ± 0.254
3.411AlaSer: 3.411 ± 0.599
3.246AlaThr: 3.246 ± 0.422
3.521AlaVal: 3.521 ± 0.415
0.495AlaTrp: 0.495 ± 0.098
2.696AlaTyr: 2.696 ± 0.257
0.0AlaXaa: 0.0 ± 0.0
Cys
0.11CysAla: 0.11 ± 0.056
0.138CysCys: 0.138 ± 0.064
0.523CysAsp: 0.523 ± 0.157
0.55CysGlu: 0.55 ± 0.159
0.358CysPhe: 0.358 ± 0.104
0.413CysGly: 0.413 ± 0.138
0.0CysHis: 0.0 ± 0.0
0.55CysIle: 0.55 ± 0.127
0.523CysLys: 0.523 ± 0.147
0.44CysLeu: 0.44 ± 0.113
0.055CysMet: 0.055 ± 0.042
0.468CysAsn: 0.468 ± 0.126
0.11CysPro: 0.11 ± 0.061
0.193CysGln: 0.193 ± 0.071
0.358CysArg: 0.358 ± 0.097
0.468CysSer: 0.468 ± 0.132
0.193CysThr: 0.193 ± 0.067
0.33CysVal: 0.33 ± 0.1
0.11CysTrp: 0.11 ± 0.046
0.358CysTyr: 0.358 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
2.613AspAla: 2.613 ± 0.534
0.385AspCys: 0.385 ± 0.129
4.593AspAsp: 4.593 ± 0.404
6.244AspGlu: 6.244 ± 0.473
4.153AspPhe: 4.153 ± 0.24
4.401AspGly: 4.401 ± 0.398
0.468AspHis: 0.468 ± 0.135
6.134AspIle: 6.134 ± 0.433
6.959AspLys: 6.959 ± 0.423
5.611AspLeu: 5.611 ± 0.504
1.87AspMet: 1.87 ± 0.205
4.951AspAsn: 4.951 ± 0.342
0.88AspPro: 0.88 ± 0.176
0.908AspGln: 0.908 ± 0.181
2.283AspArg: 2.283 ± 0.258
4.703AspSer: 4.703 ± 0.404
3.658AspThr: 3.658 ± 0.371
4.263AspVal: 4.263 ± 0.375
0.88AspTrp: 0.88 ± 0.171
3.136AspTyr: 3.136 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
4.043GluAla: 4.043 ± 0.443
0.495GluCys: 0.495 ± 0.149
5.529GluAsp: 5.529 ± 0.547
6.766GluGlu: 6.766 ± 0.627
3.438GluPhe: 3.438 ± 0.431
3.301GluGly: 3.301 ± 0.28
1.375GluHis: 1.375 ± 0.246
6.904GluIle: 6.904 ± 0.465
7.509GluLys: 7.509 ± 0.614
7.784GluLeu: 7.784 ± 0.429
2.173GluMet: 2.173 ± 0.216
5.144GluAsn: 5.144 ± 0.418
1.045GluPro: 1.045 ± 0.183
2.806GluGln: 2.806 ± 0.257
2.503GluArg: 2.503 ± 0.302
5.226GluSer: 5.226 ± 0.362
4.373GluThr: 4.373 ± 0.36
5.006GluVal: 5.006 ± 0.405
0.99GluTrp: 0.99 ± 0.186
4.263GluTyr: 4.263 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
2.035PheAla: 2.035 ± 0.307
0.468PheCys: 0.468 ± 0.149
3.631PheAsp: 3.631 ± 0.346
3.961PheGlu: 3.961 ± 0.378
1.155PhePhe: 1.155 ± 0.222
2.806PheGly: 2.806 ± 0.273
0.495PheHis: 0.495 ± 0.119
2.998PheIle: 2.998 ± 0.329
4.511PheLys: 4.511 ± 0.346
3.438PheLeu: 3.438 ± 0.339
1.403PheMet: 1.403 ± 0.202
3.466PheAsn: 3.466 ± 0.344
0.798PhePro: 0.798 ± 0.148
1.21PheGln: 1.21 ± 0.171
1.65PheArg: 1.65 ± 0.287
2.971PheSer: 2.971 ± 0.322
2.696PheThr: 2.696 ± 0.269
2.641PheVal: 2.641 ± 0.277
0.385PheTrp: 0.385 ± 0.084
2.09PheTyr: 2.09 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
2.338GlyAla: 2.338 ± 0.482
0.248GlyCys: 0.248 ± 0.07
3.218GlyAsp: 3.218 ± 0.3
4.126GlyGlu: 4.126 ± 0.318
3.383GlyPhe: 3.383 ± 0.261
3.631GlyGly: 3.631 ± 0.592
0.825GlyHis: 0.825 ± 0.162
4.979GlyIle: 4.979 ± 0.477
4.758GlyLys: 4.758 ± 0.352
4.924GlyLeu: 4.924 ± 0.377
1.623GlyMet: 1.623 ± 0.189
3.741GlyAsn: 3.741 ± 0.296
0.0GlyPro: 0.0 ± 0.0
1.293GlyGln: 1.293 ± 0.17
2.173GlyArg: 2.173 ± 0.248
3.383GlySer: 3.383 ± 0.324
3.631GlyThr: 3.631 ± 0.432
3.933GlyVal: 3.933 ± 0.298
0.66GlyTrp: 0.66 ± 0.139
2.971GlyTyr: 2.971 ± 0.281
0.0GlyXaa: 0.0 ± 0.0
His
0.578HisAla: 0.578 ± 0.132
0.11HisCys: 0.11 ± 0.058
1.183HisAsp: 1.183 ± 0.19
0.935HisGlu: 0.935 ± 0.215
0.688HisPhe: 0.688 ± 0.161
1.128HisGly: 1.128 ± 0.203
0.413HisHis: 0.413 ± 0.157
1.183HisIle: 1.183 ± 0.202
1.32HisLys: 1.32 ± 0.178
1.1HisLeu: 1.1 ± 0.212
0.275HisMet: 0.275 ± 0.085
0.963HisAsn: 0.963 ± 0.225
0.358HisPro: 0.358 ± 0.091
0.468HisGln: 0.468 ± 0.112
0.55HisArg: 0.55 ± 0.131
0.908HisSer: 0.908 ± 0.145
0.743HisThr: 0.743 ± 0.134
0.55HisVal: 0.55 ± 0.124
0.193HisTrp: 0.193 ± 0.072
0.715HisTyr: 0.715 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
4.318IleAla: 4.318 ± 0.392
0.495IleCys: 0.495 ± 0.131
5.941IleAsp: 5.941 ± 0.351
6.354IleGlu: 6.354 ± 0.446
3.163IlePhe: 3.163 ± 0.367
3.301IleGly: 3.301 ± 0.272
1.073IleHis: 1.073 ± 0.167
5.556IleIle: 5.556 ± 0.538
8.362IleLys: 8.362 ± 0.386
5.831IleLeu: 5.831 ± 0.478
1.293IleMet: 1.293 ± 0.183
5.556IleAsn: 5.556 ± 0.372
2.613IlePro: 2.613 ± 0.327
2.421IleGln: 2.421 ± 0.396
2.393IleArg: 2.393 ± 0.274
5.529IleSer: 5.529 ± 0.363
4.538IleThr: 4.538 ± 0.387
5.199IleVal: 5.199 ± 0.401
0.715IleTrp: 0.715 ± 0.142
2.833IleTyr: 2.833 ± 0.332
0.0IleXaa: 0.0 ± 0.0
Lys
5.144LysAla: 5.144 ± 0.502
0.468LysCys: 0.468 ± 0.139
7.427LysAsp: 7.427 ± 0.437
10.095LysGlu: 10.095 ± 0.657
3.878LysPhe: 3.878 ± 0.348
4.621LysGly: 4.621 ± 0.299
1.458LysHis: 1.458 ± 0.225
7.207LysIle: 7.207 ± 0.367
7.592LysLys: 7.592 ± 0.504
8.224LysLeu: 8.224 ± 0.47
2.668LysMet: 2.668 ± 0.307
6.051LysAsn: 6.051 ± 0.41
1.953LysPro: 1.953 ± 0.222
3.493LysGln: 3.493 ± 0.315
3.411LysArg: 3.411 ± 0.265
4.758LysSer: 4.758 ± 0.461
5.639LysThr: 5.639 ± 0.383
5.116LysVal: 5.116 ± 0.301
1.045LysTrp: 1.045 ± 0.152
3.796LysTyr: 3.796 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
4.758LeuAla: 4.758 ± 0.367
0.523LeuCys: 0.523 ± 0.134
6.161LeuAsp: 6.161 ± 0.432
6.381LeuGlu: 6.381 ± 0.475
3.796LeuPhe: 3.796 ± 0.514
4.896LeuGly: 4.896 ± 0.481
1.1LeuHis: 1.1 ± 0.222
5.941LeuIle: 5.941 ± 0.441
8.307LeuLys: 8.307 ± 0.537
6.216LeuLeu: 6.216 ± 0.465
2.09LeuMet: 2.09 ± 0.274
5.501LeuAsn: 5.501 ± 0.373
2.09LeuPro: 2.09 ± 0.231
2.531LeuGln: 2.531 ± 0.295
2.723LeuArg: 2.723 ± 0.248
6.491LeuSer: 6.491 ± 0.368
4.869LeuThr: 4.869 ± 0.394
4.373LeuVal: 4.373 ± 0.378
0.66LeuTrp: 0.66 ± 0.124
3.301LeuTyr: 3.301 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
1.76MetAla: 1.76 ± 0.222
0.055MetCys: 0.055 ± 0.04
1.65MetAsp: 1.65 ± 0.234
1.98MetGlu: 1.98 ± 0.229
1.155MetPhe: 1.155 ± 0.178
1.293MetGly: 1.293 ± 0.187
0.193MetHis: 0.193 ± 0.077
1.925MetIle: 1.925 ± 0.201
2.421MetLys: 2.421 ± 0.212
1.678MetLeu: 1.678 ± 0.188
0.715MetMet: 0.715 ± 0.114
2.035MetAsn: 2.035 ± 0.24
0.715MetPro: 0.715 ± 0.126
0.935MetGln: 0.935 ± 0.236
0.853MetArg: 0.853 ± 0.166
1.843MetSer: 1.843 ± 0.325
1.925MetThr: 1.925 ± 0.191
1.623MetVal: 1.623 ± 0.185
0.248MetTrp: 0.248 ± 0.084
1.155MetTyr: 1.155 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
3.383AsnAla: 3.383 ± 0.345
0.44AsnCys: 0.44 ± 0.131
3.411AsnAsp: 3.411 ± 0.325
4.814AsnGlu: 4.814 ± 0.372
2.888AsnPhe: 2.888 ± 0.295
4.621AsnGly: 4.621 ± 0.336
0.963AsnHis: 0.963 ± 0.178
5.639AsnIle: 5.639 ± 0.418
6.106AsnLys: 6.106 ± 0.419
5.556AsnLeu: 5.556 ± 0.452
1.678AsnMet: 1.678 ± 0.194
4.043AsnAsn: 4.043 ± 0.335
1.953AsnPro: 1.953 ± 0.209
3.163AsnGln: 3.163 ± 0.345
2.255AsnArg: 2.255 ± 0.285
4.318AsnSer: 4.318 ± 0.456
3.191AsnThr: 3.191 ± 0.257
3.906AsnVal: 3.906 ± 0.247
0.633AsnTrp: 0.633 ± 0.124
2.861AsnTyr: 2.861 ± 0.321
0.0AsnXaa: 0.0 ± 0.0
Pro
1.073ProAla: 1.073 ± 0.16
0.083ProCys: 0.083 ± 0.047
1.705ProAsp: 1.705 ± 0.225
1.513ProGlu: 1.513 ± 0.181
1.073ProPhe: 1.073 ± 0.199
0.11ProGly: 0.11 ± 0.059
0.358ProHis: 0.358 ± 0.087
1.623ProIle: 1.623 ± 0.181
2.035ProLys: 2.035 ± 0.311
1.87ProLeu: 1.87 ± 0.237
0.633ProMet: 0.633 ± 0.15
1.568ProAsn: 1.568 ± 0.189
0.413ProPro: 0.413 ± 0.093
0.715ProGln: 0.715 ± 0.124
0.688ProArg: 0.688 ± 0.134
1.705ProSer: 1.705 ± 0.267
1.623ProThr: 1.623 ± 0.235
1.705ProVal: 1.705 ± 0.255
0.083ProTrp: 0.083 ± 0.041
1.238ProTyr: 1.238 ± 0.206
0.0ProXaa: 0.0 ± 0.0
Gln
2.228GlnAla: 2.228 ± 0.419
0.193GlnCys: 0.193 ± 0.081
1.65GlnAsp: 1.65 ± 0.21
2.365GlnGlu: 2.365 ± 0.235
1.348GlnPhe: 1.348 ± 0.192
1.678GlnGly: 1.678 ± 0.201
0.66GlnHis: 0.66 ± 0.148
2.118GlnIle: 2.118 ± 0.226
3.108GlnLys: 3.108 ± 0.455
3.521GlnLeu: 3.521 ± 0.41
1.32GlnMet: 1.32 ± 0.256
1.98GlnAsn: 1.98 ± 0.254
0.743GlnPro: 0.743 ± 0.144
1.375GlnGln: 1.375 ± 0.417
1.76GlnArg: 1.76 ± 0.204
1.98GlnSer: 1.98 ± 0.283
1.953GlnThr: 1.953 ± 0.343
1.513GlnVal: 1.513 ± 0.203
0.413GlnTrp: 0.413 ± 0.097
1.403GlnTyr: 1.403 ± 0.172
0.0GlnXaa: 0.0 ± 0.0
Arg
1.32ArgAla: 1.32 ± 0.18
0.138ArgCys: 0.138 ± 0.061
2.641ArgAsp: 2.641 ± 0.214
3.246ArgGlu: 3.246 ± 0.318
1.485ArgPhe: 1.485 ± 0.223
2.035ArgGly: 2.035 ± 0.236
0.578ArgHis: 0.578 ± 0.116
2.613ArgIle: 2.613 ± 0.315
3.246ArgLys: 3.246 ± 0.345
2.668ArgLeu: 2.668 ± 0.301
0.825ArgMet: 0.825 ± 0.17
2.531ArgAsn: 2.531 ± 0.257
0.88ArgPro: 0.88 ± 0.172
1.128ArgGln: 1.128 ± 0.173
1.128ArgArg: 1.128 ± 0.173
1.87ArgSer: 1.87 ± 0.26
2.228ArgThr: 2.228 ± 0.24
2.173ArgVal: 2.173 ± 0.23
0.633ArgTrp: 0.633 ± 0.154
2.063ArgTyr: 2.063 ± 0.212
0.0ArgXaa: 0.0 ± 0.0
Ser
3.548SerAla: 3.548 ± 0.523
0.385SerCys: 0.385 ± 0.107
4.483SerAsp: 4.483 ± 0.392
5.226SerGlu: 5.226 ± 0.379
3.576SerPhe: 3.576 ± 0.349
4.016SerGly: 4.016 ± 0.412
0.77SerHis: 0.77 ± 0.159
5.199SerIle: 5.199 ± 0.404
6.244SerLys: 6.244 ± 0.501
5.226SerLeu: 5.226 ± 0.477
1.513SerMet: 1.513 ± 0.174
3.823SerAsn: 3.823 ± 0.405
1.485SerPro: 1.485 ± 0.194
1.898SerGln: 1.898 ± 0.249
2.696SerArg: 2.696 ± 0.243
4.731SerSer: 4.731 ± 0.744
3.466SerThr: 3.466 ± 0.287
3.988SerVal: 3.988 ± 0.353
0.688SerTrp: 0.688 ± 0.141
2.806SerTyr: 2.806 ± 0.308
0.0SerXaa: 0.0 ± 0.0
Thr
3.218ThrAla: 3.218 ± 0.465
0.44ThrCys: 0.44 ± 0.141
3.906ThrAsp: 3.906 ± 0.302
3.631ThrGlu: 3.631 ± 0.351
2.586ThrPhe: 2.586 ± 0.288
3.548ThrGly: 3.548 ± 0.43
1.155ThrHis: 1.155 ± 0.205
4.511ThrIle: 4.511 ± 0.413
5.419ThrLys: 5.419 ± 0.34
5.254ThrLeu: 5.254 ± 0.339
1.458ThrMet: 1.458 ± 0.203
3.466ThrAsn: 3.466 ± 0.321
1.76ThrPro: 1.76 ± 0.184
1.87ThrGln: 1.87 ± 0.302
2.008ThrArg: 2.008 ± 0.225
3.851ThrSer: 3.851 ± 0.315
3.411ThrThr: 3.411 ± 0.378
4.566ThrVal: 4.566 ± 0.466
0.578ThrTrp: 0.578 ± 0.153
2.393ThrTyr: 2.393 ± 0.228
0.0ThrXaa: 0.0 ± 0.0
Val
3.576ValAla: 3.576 ± 0.388
0.44ValCys: 0.44 ± 0.154
4.786ValAsp: 4.786 ± 0.314
4.896ValGlu: 4.896 ± 0.506
2.916ValPhe: 2.916 ± 0.251
3.273ValGly: 3.273 ± 0.26
0.853ValHis: 0.853 ± 0.154
4.483ValIle: 4.483 ± 0.436
5.749ValLys: 5.749 ± 0.348
4.153ValLeu: 4.153 ± 0.375
1.458ValMet: 1.458 ± 0.235
3.658ValAsn: 3.658 ± 0.395
1.513ValPro: 1.513 ± 0.24
2.365ValGln: 2.365 ± 0.225
1.87ValArg: 1.87 ± 0.221
4.016ValSer: 4.016 ± 0.375
4.181ValThr: 4.181 ± 0.368
4.263ValVal: 4.263 ± 0.369
0.908ValTrp: 0.908 ± 0.193
2.916ValTyr: 2.916 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.468TrpAla: 0.468 ± 0.111
0.138TrpCys: 0.138 ± 0.06
0.798TrpAsp: 0.798 ± 0.126
0.798TrpGlu: 0.798 ± 0.166
0.385TrpPhe: 0.385 ± 0.12
0.523TrpGly: 0.523 ± 0.133
0.165TrpHis: 0.165 ± 0.065
0.633TrpIle: 0.633 ± 0.168
0.935TrpLys: 0.935 ± 0.153
0.935TrpLeu: 0.935 ± 0.144
0.55TrpMet: 0.55 ± 0.117
1.045TrpAsn: 1.045 ± 0.166
0.0TrpPro: 0.0 ± 0.0
0.33TrpGln: 0.33 ± 0.09
0.303TrpArg: 0.303 ± 0.107
0.523TrpSer: 0.523 ± 0.118
0.88TrpThr: 0.88 ± 0.172
0.77TrpVal: 0.77 ± 0.169
0.083TrpTrp: 0.083 ± 0.05
0.468TrpTyr: 0.468 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.365TyrAla: 2.365 ± 0.246
0.44TyrCys: 0.44 ± 0.115
2.943TyrAsp: 2.943 ± 0.24
3.576TyrGlu: 3.576 ± 0.383
1.705TyrPhe: 1.705 ± 0.252
2.778TyrGly: 2.778 ± 0.222
0.77TyrHis: 0.77 ± 0.154
3.548TyrIle: 3.548 ± 0.388
3.933TyrLys: 3.933 ± 0.328
3.686TyrLeu: 3.686 ± 0.351
0.99TyrMet: 0.99 ± 0.165
2.806TyrAsn: 2.806 ± 0.3
1.458TyrPro: 1.458 ± 0.262
1.678TyrGln: 1.678 ± 0.188
1.953TyrArg: 1.953 ± 0.263
3.081TyrSer: 3.081 ± 0.312
2.586TyrThr: 2.586 ± 0.24
2.833TyrVal: 2.833 ± 0.276
0.33TyrTrp: 0.33 ± 0.102
2.008TyrTyr: 2.008 ± 0.267
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 178 proteins (36357 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski