Amino acid dipepetide frequency for Microbacterium phage Percival

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.85AlaAla: 22.85 ± 2.151
0.342AlaCys: 0.342 ± 0.146
8.346AlaAsp: 8.346 ± 0.78
10.536AlaGlu: 10.536 ± 1.095
4.31AlaPhe: 4.31 ± 0.701
8.962AlaGly: 8.962 ± 0.964
2.394AlaHis: 2.394 ± 0.331
6.294AlaIle: 6.294 ± 0.81
5.268AlaLys: 5.268 ± 0.574
14.162AlaLeu: 14.162 ± 1.146
3.489AlaMet: 3.489 ± 0.399
3.147AlaAsn: 3.147 ± 0.396
6.773AlaPro: 6.773 ± 0.73
3.558AlaGln: 3.558 ± 0.586
10.741AlaArg: 10.741 ± 1.178
7.115AlaSer: 7.115 ± 0.829
7.868AlaThr: 7.868 ± 0.798
8.894AlaVal: 8.894 ± 0.788
2.189AlaTrp: 2.189 ± 0.364
3.489AlaTyr: 3.489 ± 0.559
0.0AlaXaa: 0.0 ± 0.0
Cys
0.547CysAla: 0.547 ± 0.196
0.0CysCys: 0.0 ± 0.0
0.274CysAsp: 0.274 ± 0.12
0.274CysGlu: 0.274 ± 0.159
0.342CysPhe: 0.342 ± 0.157
0.547CysGly: 0.547 ± 0.205
0.342CysHis: 0.342 ± 0.144
0.41CysIle: 0.41 ± 0.142
0.205CysLys: 0.205 ± 0.126
0.068CysLeu: 0.068 ± 0.075
0.068CysMet: 0.068 ± 0.075
0.137CysAsn: 0.137 ± 0.107
0.479CysPro: 0.479 ± 0.231
0.342CysGln: 0.342 ± 0.148
0.205CysArg: 0.205 ± 0.124
0.274CysSer: 0.274 ± 0.136
0.068CysThr: 0.068 ± 0.059
0.137CysVal: 0.137 ± 0.079
0.068CysTrp: 0.068 ± 0.079
0.137CysTyr: 0.137 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
9.509AspAla: 9.509 ± 0.987
0.205AspCys: 0.205 ± 0.132
4.105AspAsp: 4.105 ± 0.604
4.31AspGlu: 4.31 ± 0.594
1.574AspPhe: 1.574 ± 0.268
5.541AspGly: 5.541 ± 0.652
1.026AspHis: 1.026 ± 0.251
2.873AspIle: 2.873 ± 0.344
1.437AspLys: 1.437 ± 0.32
6.978AspLeu: 6.978 ± 0.758
0.958AspMet: 0.958 ± 0.236
1.026AspAsn: 1.026 ± 0.24
4.652AspPro: 4.652 ± 0.549
1.574AspGln: 1.574 ± 0.262
5.063AspArg: 5.063 ± 0.834
2.463AspSer: 2.463 ± 0.42
4.31AspThr: 4.31 ± 0.521
3.352AspVal: 3.352 ± 0.432
1.095AspTrp: 1.095 ± 0.278
1.437AspTyr: 1.437 ± 0.299
0.0AspXaa: 0.0 ± 0.0
Glu
10.399GluAla: 10.399 ± 1.024
0.41GluCys: 0.41 ± 0.242
3.558GluAsp: 3.558 ± 0.485
4.378GluGlu: 4.378 ± 0.663
1.231GluPhe: 1.231 ± 0.325
5.063GluGly: 5.063 ± 0.574
1.779GluHis: 1.779 ± 0.307
1.437GluIle: 1.437 ± 0.255
1.505GluLys: 1.505 ± 0.443
5.815GluLeu: 5.815 ± 0.595
1.163GluMet: 1.163 ± 0.297
1.505GluAsn: 1.505 ± 0.279
1.574GluPro: 1.574 ± 0.406
1.71GluGln: 1.71 ± 0.305
5.473GluArg: 5.473 ± 0.684
1.642GluSer: 1.642 ± 0.355
4.173GluThr: 4.173 ± 0.54
4.994GluVal: 4.994 ± 0.5
1.095GluTrp: 1.095 ± 0.276
0.958GluTyr: 0.958 ± 0.25
0.0GluXaa: 0.0 ± 0.0
Phe
3.694PheAla: 3.694 ± 0.471
0.137PheCys: 0.137 ± 0.088
2.189PheAsp: 2.189 ± 0.305
1.984PheGlu: 1.984 ± 0.344
1.095PhePhe: 1.095 ± 0.375
2.668PheGly: 2.668 ± 0.364
0.547PheHis: 0.547 ± 0.195
1.163PheIle: 1.163 ± 0.288
0.616PheLys: 0.616 ± 0.176
2.805PheLeu: 2.805 ± 0.544
0.684PheMet: 0.684 ± 0.218
0.616PheAsn: 0.616 ± 0.199
1.71PhePro: 1.71 ± 0.292
0.958PheGln: 0.958 ± 0.222
2.121PheArg: 2.121 ± 0.394
1.505PheSer: 1.505 ± 0.36
2.531PheThr: 2.531 ± 0.389
1.437PheVal: 1.437 ± 0.295
0.137PheTrp: 0.137 ± 0.109
0.958PheTyr: 0.958 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
7.525GlyAla: 7.525 ± 0.736
0.41GlyCys: 0.41 ± 0.168
4.515GlyAsp: 4.515 ± 0.63
4.173GlyGlu: 4.173 ± 0.477
3.558GlyPhe: 3.558 ± 0.479
6.02GlyGly: 6.02 ± 0.785
1.368GlyHis: 1.368 ± 0.378
5.063GlyIle: 5.063 ± 1.199
3.352GlyLys: 3.352 ± 0.526
7.047GlyLeu: 7.047 ± 0.532
1.026GlyMet: 1.026 ± 0.231
2.052GlyAsn: 2.052 ± 0.377
3.9GlyPro: 3.9 ± 0.526
2.942GlyGln: 2.942 ± 0.442
4.036GlyArg: 4.036 ± 0.534
4.994GlySer: 4.994 ± 0.689
5.952GlyThr: 5.952 ± 0.749
6.568GlyVal: 6.568 ± 0.672
2.668GlyTrp: 2.668 ± 0.37
2.668GlyTyr: 2.668 ± 0.459
0.0GlyXaa: 0.0 ± 0.0
His
2.121HisAla: 2.121 ± 0.45
0.0HisCys: 0.0 ± 0.0
1.779HisAsp: 1.779 ± 0.349
1.574HisGlu: 1.574 ± 0.371
0.821HisPhe: 0.821 ± 0.315
2.258HisGly: 2.258 ± 0.37
0.753HisHis: 0.753 ± 0.235
0.479HisIle: 0.479 ± 0.145
0.479HisLys: 0.479 ± 0.18
2.942HisLeu: 2.942 ± 0.399
0.547HisMet: 0.547 ± 0.194
0.616HisAsn: 0.616 ± 0.185
1.71HisPro: 1.71 ± 0.357
0.547HisGln: 0.547 ± 0.194
0.958HisArg: 0.958 ± 0.343
1.026HisSer: 1.026 ± 0.239
0.821HisThr: 0.821 ± 0.223
1.574HisVal: 1.574 ± 0.275
0.479HisTrp: 0.479 ± 0.164
0.479HisTyr: 0.479 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
5.405IleAla: 5.405 ± 0.521
0.274IleCys: 0.274 ± 0.165
2.668IleAsp: 2.668 ± 0.405
2.121IleGlu: 2.121 ± 0.368
1.026IlePhe: 1.026 ± 0.283
3.694IleGly: 3.694 ± 0.561
1.163IleHis: 1.163 ± 0.253
1.368IleIle: 1.368 ± 0.387
1.642IleLys: 1.642 ± 0.397
2.873IleLeu: 2.873 ± 0.458
0.41IleMet: 0.41 ± 0.15
0.889IleAsn: 0.889 ± 0.247
2.873IlePro: 2.873 ± 0.31
1.505IleGln: 1.505 ± 0.273
3.01IleArg: 3.01 ± 0.444
2.394IleSer: 2.394 ± 0.442
3.284IleThr: 3.284 ± 0.566
3.831IleVal: 3.831 ± 0.62
1.163IleTrp: 1.163 ± 0.491
0.821IleTyr: 0.821 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
4.721LysAla: 4.721 ± 0.876
0.137LysCys: 0.137 ± 0.09
1.984LysAsp: 1.984 ± 0.275
1.847LysGlu: 1.847 ± 0.321
0.41LysPhe: 0.41 ± 0.154
2.668LysGly: 2.668 ± 0.386
0.753LysHis: 0.753 ± 0.199
1.095LysIle: 1.095 ± 0.253
1.574LysLys: 1.574 ± 0.325
2.531LysLeu: 2.531 ± 0.467
0.684LysMet: 0.684 ± 0.186
0.684LysAsn: 0.684 ± 0.206
1.779LysPro: 1.779 ± 0.33
0.41LysGln: 0.41 ± 0.17
2.668LysArg: 2.668 ± 0.411
1.847LysSer: 1.847 ± 0.317
2.668LysThr: 2.668 ± 0.421
3.01LysVal: 3.01 ± 0.559
0.41LysTrp: 0.41 ± 0.163
0.684LysTyr: 0.684 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
14.572LeuAla: 14.572 ± 0.961
0.342LeuCys: 0.342 ± 0.155
5.678LeuAsp: 5.678 ± 0.555
4.105LeuGlu: 4.105 ± 0.544
1.847LeuPhe: 1.847 ± 0.355
6.157LeuGly: 6.157 ± 0.734
1.847LeuHis: 1.847 ± 0.45
3.9LeuIle: 3.9 ± 0.522
2.531LeuLys: 2.531 ± 0.409
6.636LeuLeu: 6.636 ± 0.803
1.095LeuMet: 1.095 ± 0.247
2.737LeuAsn: 2.737 ± 0.539
5.473LeuPro: 5.473 ± 0.629
3.626LeuGln: 3.626 ± 0.43
8.483LeuArg: 8.483 ± 1.067
6.294LeuSer: 6.294 ± 0.614
5.952LeuThr: 5.952 ± 0.558
4.994LeuVal: 4.994 ± 0.458
1.231LeuTrp: 1.231 ± 0.325
1.916LeuTyr: 1.916 ± 0.374
0.0LeuXaa: 0.0 ± 0.0
Met
3.352MetAla: 3.352 ± 0.444
0.205MetCys: 0.205 ± 0.106
1.163MetAsp: 1.163 ± 0.289
0.958MetGlu: 0.958 ± 0.285
0.274MetPhe: 0.274 ± 0.157
0.821MetGly: 0.821 ± 0.233
0.342MetHis: 0.342 ± 0.133
0.342MetIle: 0.342 ± 0.141
0.205MetLys: 0.205 ± 0.114
1.231MetLeu: 1.231 ± 0.232
0.205MetMet: 0.205 ± 0.104
0.547MetAsn: 0.547 ± 0.21
1.437MetPro: 1.437 ± 0.253
0.547MetGln: 0.547 ± 0.162
1.163MetArg: 1.163 ± 0.273
1.847MetSer: 1.847 ± 0.397
2.805MetThr: 2.805 ± 0.492
0.821MetVal: 0.821 ± 0.269
0.137MetTrp: 0.137 ± 0.104
0.068MetTyr: 0.068 ± 0.059
0.0MetXaa: 0.0 ± 0.0
Asn
3.694AsnAla: 3.694 ± 0.636
0.068AsnCys: 0.068 ± 0.059
1.231AsnAsp: 1.231 ± 0.248
0.958AsnGlu: 0.958 ± 0.26
0.684AsnPhe: 0.684 ± 0.236
3.694AsnGly: 3.694 ± 0.568
0.547AsnHis: 0.547 ± 0.182
0.889AsnIle: 0.889 ± 0.295
0.41AsnLys: 0.41 ± 0.201
2.6AsnLeu: 2.6 ± 0.446
0.342AsnMet: 0.342 ± 0.135
0.274AsnAsn: 0.274 ± 0.149
1.574AsnPro: 1.574 ± 0.277
0.684AsnGln: 0.684 ± 0.199
1.163AsnArg: 1.163 ± 0.328
1.437AsnSer: 1.437 ± 0.363
1.3AsnThr: 1.3 ± 0.265
1.505AsnVal: 1.505 ± 0.414
0.547AsnTrp: 0.547 ± 0.232
0.479AsnTyr: 0.479 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
6.978ProAla: 6.978 ± 0.656
0.274ProCys: 0.274 ± 0.122
3.352ProAsp: 3.352 ± 0.395
3.626ProGlu: 3.626 ± 0.51
1.71ProPhe: 1.71 ± 0.387
5.199ProGly: 5.199 ± 0.542
1.437ProHis: 1.437 ± 0.316
2.805ProIle: 2.805 ± 0.465
1.437ProLys: 1.437 ± 0.298
4.105ProLeu: 4.105 ± 0.698
1.3ProMet: 1.3 ± 0.243
1.3ProAsn: 1.3 ± 0.23
2.052ProPro: 2.052 ± 0.413
1.984ProGln: 1.984 ± 0.314
3.489ProArg: 3.489 ± 0.538
3.147ProSer: 3.147 ± 0.51
4.242ProThr: 4.242 ± 0.489
4.173ProVal: 4.173 ± 0.606
0.479ProTrp: 0.479 ± 0.193
1.437ProTyr: 1.437 ± 0.294
0.0ProXaa: 0.0 ± 0.0
Gln
4.036GlnAla: 4.036 ± 0.498
0.342GlnCys: 0.342 ± 0.171
1.437GlnAsp: 1.437 ± 0.367
1.437GlnGlu: 1.437 ± 0.263
0.137GlnPhe: 0.137 ± 0.079
2.6GlnGly: 2.6 ± 0.587
1.026GlnHis: 1.026 ± 0.236
0.753GlnIle: 0.753 ± 0.181
1.3GlnLys: 1.3 ± 0.421
3.352GlnLeu: 3.352 ± 0.551
0.547GlnMet: 0.547 ± 0.173
0.547GlnAsn: 0.547 ± 0.164
1.847GlnPro: 1.847 ± 0.398
0.753GlnGln: 0.753 ± 0.231
3.01GlnArg: 3.01 ± 0.577
0.889GlnSer: 0.889 ± 0.213
1.984GlnThr: 1.984 ± 0.279
2.258GlnVal: 2.258 ± 0.354
0.41GlnTrp: 0.41 ± 0.183
0.616GlnTyr: 0.616 ± 0.202
0.0GlnXaa: 0.0 ± 0.0
Arg
8.346ArgAla: 8.346 ± 0.877
0.821ArgCys: 0.821 ± 0.252
6.636ArgAsp: 6.636 ± 0.686
4.789ArgGlu: 4.789 ± 0.565
2.805ArgPhe: 2.805 ± 0.476
3.763ArgGly: 3.763 ± 0.447
1.574ArgHis: 1.574 ± 0.353
2.6ArgIle: 2.6 ± 0.478
3.694ArgLys: 3.694 ± 0.498
6.294ArgLeu: 6.294 ± 0.654
1.642ArgMet: 1.642 ± 0.326
1.368ArgAsn: 1.368 ± 0.305
3.558ArgPro: 3.558 ± 0.567
2.326ArgGln: 2.326 ± 0.445
6.705ArgArg: 6.705 ± 0.927
3.626ArgSer: 3.626 ± 0.483
3.968ArgThr: 3.968 ± 0.516
5.199ArgVal: 5.199 ± 0.644
1.437ArgTrp: 1.437 ± 0.347
2.463ArgTyr: 2.463 ± 0.442
0.0ArgXaa: 0.0 ± 0.0
Ser
8.62SerAla: 8.62 ± 0.967
0.137SerCys: 0.137 ± 0.113
3.079SerAsp: 3.079 ± 0.401
2.668SerGlu: 2.668 ± 0.484
1.779SerPhe: 1.779 ± 0.332
5.405SerGly: 5.405 ± 0.751
1.231SerHis: 1.231 ± 0.246
2.463SerIle: 2.463 ± 0.407
1.71SerLys: 1.71 ± 0.289
4.242SerLeu: 4.242 ± 0.562
1.163SerMet: 1.163 ± 0.261
1.231SerAsn: 1.231 ± 0.301
2.942SerPro: 2.942 ± 0.405
1.095SerGln: 1.095 ± 0.242
3.831SerArg: 3.831 ± 0.5
3.147SerSer: 3.147 ± 0.577
3.626SerThr: 3.626 ± 0.51
3.9SerVal: 3.9 ± 0.43
0.958SerTrp: 0.958 ± 0.251
1.642SerTyr: 1.642 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
9.715ThrAla: 9.715 ± 1.058
0.205ThrCys: 0.205 ± 0.118
4.926ThrAsp: 4.926 ± 0.565
2.942ThrGlu: 2.942 ± 0.499
2.737ThrPhe: 2.737 ± 0.432
5.952ThrGly: 5.952 ± 0.734
1.3ThrHis: 1.3 ± 0.288
2.805ThrIle: 2.805 ± 0.458
1.847ThrLys: 1.847 ± 0.316
5.815ThrLeu: 5.815 ± 0.53
1.026ThrMet: 1.026 ± 0.197
1.779ThrAsn: 1.779 ± 0.341
5.131ThrPro: 5.131 ± 0.667
1.368ThrGln: 1.368 ± 0.28
4.652ThrArg: 4.652 ± 0.639
4.105ThrSer: 4.105 ± 0.49
5.952ThrThr: 5.952 ± 0.697
4.926ThrVal: 4.926 ± 0.513
1.095ThrTrp: 1.095 ± 0.3
1.71ThrTyr: 1.71 ± 0.316
0.0ThrXaa: 0.0 ± 0.0
Val
9.373ValAla: 9.373 ± 0.792
0.41ValCys: 0.41 ± 0.209
3.421ValAsp: 3.421 ± 0.413
4.515ValGlu: 4.515 ± 0.625
2.052ValPhe: 2.052 ± 0.364
4.515ValGly: 4.515 ± 0.743
1.3ValHis: 1.3 ± 0.321
3.9ValIle: 3.9 ± 0.4
2.326ValLys: 2.326 ± 0.507
6.089ValLeu: 6.089 ± 0.692
1.3ValMet: 1.3 ± 0.312
1.984ValAsn: 1.984 ± 0.378
3.9ValPro: 3.9 ± 0.449
2.052ValGln: 2.052 ± 0.383
4.378ValArg: 4.378 ± 0.619
4.789ValSer: 4.789 ± 0.565
4.857ValThr: 4.857 ± 0.61
4.378ValVal: 4.378 ± 0.627
1.3ValTrp: 1.3 ± 0.318
1.779ValTyr: 1.779 ± 0.361
0.0ValXaa: 0.0 ± 0.0
Trp
2.258TrpAla: 2.258 ± 0.361
0.0TrpCys: 0.0 ± 0.0
1.368TrpAsp: 1.368 ± 0.287
1.505TrpGlu: 1.505 ± 0.422
0.342TrpPhe: 0.342 ± 0.128
1.505TrpGly: 1.505 ± 0.349
0.616TrpHis: 0.616 ± 0.169
0.958TrpIle: 0.958 ± 0.181
0.479TrpLys: 0.479 ± 0.188
1.505TrpLeu: 1.505 ± 0.354
0.137TrpMet: 0.137 ± 0.088
1.095TrpAsn: 1.095 ± 0.494
0.342TrpPro: 0.342 ± 0.141
0.616TrpGln: 0.616 ± 0.171
0.684TrpArg: 0.684 ± 0.219
0.958TrpSer: 0.958 ± 0.283
1.642TrpThr: 1.642 ± 0.382
0.821TrpVal: 0.821 ± 0.215
0.137TrpTrp: 0.137 ± 0.089
0.274TrpTyr: 0.274 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.01TyrAla: 3.01 ± 0.485
0.205TyrCys: 0.205 ± 0.13
1.779TyrAsp: 1.779 ± 0.368
1.163TyrGlu: 1.163 ± 0.283
0.821TyrPhe: 0.821 ± 0.218
2.805TyrGly: 2.805 ± 0.453
0.616TyrHis: 0.616 ± 0.294
0.821TyrIle: 0.821 ± 0.218
0.547TyrLys: 0.547 ± 0.229
2.326TyrLeu: 2.326 ± 0.332
0.616TyrMet: 0.616 ± 0.224
0.547TyrAsn: 0.547 ± 0.158
0.821TyrPro: 0.821 ± 0.27
0.684TyrGln: 0.684 ± 0.191
1.847TyrArg: 1.847 ± 0.331
1.368TyrSer: 1.368 ± 0.349
1.984TyrThr: 1.984 ± 0.331
1.847TyrVal: 1.847 ± 0.408
0.205TyrTrp: 0.205 ± 0.101
0.547TyrTyr: 0.547 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski