Nocardioides sp. GY 10113

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4T0V9S9|A0A4T0V9S9_9ACTN Indole-3-glycerol phosphate synthase OS=Nocardioides sp. GY 10113 OX=2569761 GN=trpC PE=3 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84ARR4 pKa = 11.84NKK6 pKa = 9.61FAAVLGGTVVMASAAIVTTTTAASAVEE33 pKa = 4.07PTGGCWVYY41 pKa = 10.07NTGGNTNLEE50 pKa = 4.3SAPTSSISSALAGWSSSPPDD70 pKa = 3.52YY71 pKa = 11.44DD72 pKa = 3.51LTTSGGTLVGEE83 pKa = 4.29TRR85 pKa = 11.84NFSLVVSAGPSSPVPATTTAYY106 pKa = 10.89YY107 pKa = 9.95YY108 pKa = 10.72FSVNGANLAPVAVADD123 pKa = 4.59IPASTNTPGGTVTGSFDD140 pKa = 3.08IAQGGTNTLVLRR152 pKa = 11.84KK153 pKa = 9.69VIYY156 pKa = 9.62DD157 pKa = 3.29IPSYY161 pKa = 9.49SVRR164 pKa = 11.84VQCNGQSSGSQTGINPATSPVDD186 pKa = 3.73TNVTASFTAFAQGVATITGITNQTVMNAARR216 pKa = 11.84AGDD219 pKa = 4.17TISFAASNFSGADD232 pKa = 3.26ANSTAEE238 pKa = 3.62ICTTDD243 pKa = 3.03GATCVGATSFGVGTNGAGTGTITVPSGVSGSMRR276 pKa = 11.84LRR278 pKa = 11.84LTSGGEE284 pKa = 3.58VGMRR288 pKa = 11.84SITILGQPTLTLSLTGGGAGTTVGVTGTDD317 pKa = 3.09WDD319 pKa = 4.15PSQTVSIGGYY329 pKa = 5.36TAPLGGFPLPSTTDD343 pKa = 3.55PVSTATASATGTLSGSFTVNDD364 pKa = 3.97ANTAYY369 pKa = 9.68ISASQRR375 pKa = 11.84SGPGAIWSDD384 pKa = 2.95AAFAFSADD392 pKa = 2.96TCTAKK397 pKa = 10.65EE398 pKa = 3.95GLEE401 pKa = 4.38DD402 pKa = 4.19NSDD405 pKa = 3.46ACSVLEE411 pKa = 4.33TIEE414 pKa = 4.35LTVLPGDD421 pKa = 3.54LTMAKK426 pKa = 10.24AAGTVEE432 pKa = 3.99MGAVQLNGTDD442 pKa = 3.54QTSTGSLQDD451 pKa = 3.53VTVMDD456 pKa = 4.07YY457 pKa = 11.12RR458 pKa = 11.84GGTLGWSLIGTFAGLTSGPQPIDD481 pKa = 3.28ADD483 pKa = 3.92KK484 pKa = 11.37LSWTPSCTPGANSDD498 pKa = 3.68DD499 pKa = 4.0TVTVGAAGAFPSATEE514 pKa = 4.06ALPLCAVAAAGFGPDD529 pKa = 3.67GVSGGDD535 pKa = 3.27VTADD539 pKa = 3.31ADD541 pKa = 4.22LSLDD545 pKa = 4.39LLINQQAGDD554 pKa = 3.75YY555 pKa = 10.68SGTLTLTLSS564 pKa = 3.51

Molecular weight: 55.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4T0V0P3|A0A4T0V0P3_9ACTN Alpha/beta hydrolase OS=Nocardioides sp. GY 10113 OX=2569761 GN=E8D34_13455 PE=4 SV=1
MM1 pKa = 7.56AGSSPRR7 pKa = 11.84WRR9 pKa = 11.84LSSRR13 pKa = 11.84SEE15 pKa = 4.33RR16 pKa = 11.84AWSWAPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84PSPRR29 pKa = 11.84RR30 pKa = 11.84PRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PTPRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84SSPRR45 pKa = 11.84RR46 pKa = 11.84WPRR49 pKa = 11.84PRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84PARR56 pKa = 11.84RR57 pKa = 11.84PSATPPGRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84SATPPSGRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84GPSSRR82 pKa = 11.84PAGRR86 pKa = 11.84PPAAPPTGG94 pKa = 3.38

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3866

0

3866

1303206

33

3357

337.1

35.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.95 ± 0.064

0.705 ± 0.01

6.413 ± 0.035

5.84 ± 0.041

2.746 ± 0.02

9.541 ± 0.036

2.08 ± 0.02

3.45 ± 0.026

1.789 ± 0.028

10.17 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.015

1.701 ± 0.022

5.766 ± 0.031

2.555 ± 0.02

7.727 ± 0.044

4.995 ± 0.029

6.018 ± 0.038

9.28 ± 0.036

1.516 ± 0.017

1.997 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski