Amino acid dipepetide frequency for Prochlorococcus phage P-SSM2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.411AlaAla: 4.411 ± 0.305
0.536AlaCys: 0.536 ± 0.085
3.825AlaAsp: 3.825 ± 0.258
3.165AlaGlu: 3.165 ± 0.235
2.106AlaPhe: 2.106 ± 0.164
5.881AlaGly: 5.881 ± 0.446
1.059AlaHis: 1.059 ± 0.132
3.825AlaIle: 3.825 ± 0.208
3.888AlaLys: 3.888 ± 0.275
4.124AlaLeu: 4.124 ± 0.292
1.109AlaMet: 1.109 ± 0.119
3.427AlaAsn: 3.427 ± 0.271
2.181AlaPro: 2.181 ± 0.174
2.044AlaGln: 2.044 ± 0.175
2.143AlaArg: 2.143 ± 0.165
4.324AlaSer: 4.324 ± 0.298
4.598AlaThr: 4.598 ± 0.375
4.099AlaVal: 4.099 ± 0.302
0.548AlaTrp: 0.548 ± 0.081
2.205AlaTyr: 2.205 ± 0.175
0.0AlaXaa: 0.0 ± 0.0
Cys
0.86CysAla: 0.86 ± 0.137
0.287CysCys: 0.287 ± 0.117
0.797CysAsp: 0.797 ± 0.099
0.835CysGlu: 0.835 ± 0.125
0.374CysPhe: 0.374 ± 0.075
0.685CysGly: 0.685 ± 0.127
0.274CysHis: 0.274 ± 0.059
0.598CysIle: 0.598 ± 0.103
0.561CysLys: 0.561 ± 0.096
0.735CysLeu: 0.735 ± 0.105
0.249CysMet: 0.249 ± 0.069
0.598CysAsn: 0.598 ± 0.1
0.399CysPro: 0.399 ± 0.074
0.349CysGln: 0.349 ± 0.072
0.349CysArg: 0.349 ± 0.079
0.797CysSer: 0.797 ± 0.118
0.66CysThr: 0.66 ± 0.083
0.76CysVal: 0.76 ± 0.106
0.15CysTrp: 0.15 ± 0.041
0.449CysTyr: 0.449 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
3.95AspAla: 3.95 ± 0.237
0.785AspCys: 0.785 ± 0.122
4.598AspAsp: 4.598 ± 0.291
4.025AspGlu: 4.025 ± 0.255
3.314AspPhe: 3.314 ± 0.24
5.856AspGly: 5.856 ± 0.363
1.059AspHis: 1.059 ± 0.133
4.448AspIle: 4.448 ± 0.231
4.012AspLys: 4.012 ± 0.242
5.009AspLeu: 5.009 ± 0.253
1.383AspMet: 1.383 ± 0.164
3.439AspAsn: 3.439 ± 0.26
3.439AspPro: 3.439 ± 0.194
1.882AspGln: 1.882 ± 0.162
2.218AspArg: 2.218 ± 0.153
4.286AspSer: 4.286 ± 0.257
4.199AspThr: 4.199 ± 0.216
4.423AspVal: 4.423 ± 0.262
1.034AspTrp: 1.034 ± 0.12
3.19AspTyr: 3.19 ± 0.207
0.0AspXaa: 0.0 ± 0.0
Glu
2.866GluAla: 2.866 ± 0.211
0.598GluCys: 0.598 ± 0.109
4.249GluAsp: 4.249 ± 0.27
4.959GluGlu: 4.959 ± 0.537
2.766GluPhe: 2.766 ± 0.187
3.888GluGly: 3.888 ± 0.215
1.171GluHis: 1.171 ± 0.155
4.498GluIle: 4.498 ± 0.257
4.311GluLys: 4.311 ± 0.357
4.76GluLeu: 4.76 ± 0.327
1.533GluMet: 1.533 ± 0.181
3.452GluAsn: 3.452 ± 0.237
1.906GluPro: 1.906 ± 0.172
2.093GluGln: 2.093 ± 0.192
2.268GluArg: 2.268 ± 0.174
3.9GluSer: 3.9 ± 0.244
3.439GluThr: 3.439 ± 0.206
4.573GluVal: 4.573 ± 0.288
0.872GluTrp: 0.872 ± 0.127
2.991GluTyr: 2.991 ± 0.206
0.0GluXaa: 0.0 ± 0.0
Phe
2.268PheAla: 2.268 ± 0.146
0.486PheCys: 0.486 ± 0.077
3.352PheAsp: 3.352 ± 0.22
2.554PheGlu: 2.554 ± 0.175
1.47PhePhe: 1.47 ± 0.154
2.729PheGly: 2.729 ± 0.224
0.735PheHis: 0.735 ± 0.114
2.367PheIle: 2.367 ± 0.187
2.741PheLys: 2.741 ± 0.197
3.053PheLeu: 3.053 ± 0.248
0.81PheMet: 0.81 ± 0.097
2.816PheAsn: 2.816 ± 0.17
1.62PhePro: 1.62 ± 0.14
1.234PheGln: 1.234 ± 0.155
1.657PheArg: 1.657 ± 0.148
3.065PheSer: 3.065 ± 0.188
3.09PheThr: 3.09 ± 0.283
2.878PheVal: 2.878 ± 0.22
0.449PheTrp: 0.449 ± 0.077
1.632PheTyr: 1.632 ± 0.149
0.0PheXaa: 0.0 ± 0.0
Gly
5.545GlyAla: 5.545 ± 0.409
0.847GlyCys: 0.847 ± 0.148
4.71GlyAsp: 4.71 ± 0.287
4.05GlyGlu: 4.05 ± 0.188
2.928GlyPhe: 2.928 ± 0.182
9.158GlyGly: 9.158 ± 1.118
1.321GlyHis: 1.321 ± 0.139
5.769GlyIle: 5.769 ± 0.464
4.747GlyLys: 4.747 ± 0.304
5.358GlyLeu: 5.358 ± 0.351
1.72GlyMet: 1.72 ± 0.192
4.909GlyAsn: 4.909 ± 0.366
4.249GlyPro: 4.249 ± 1.113
2.442GlyGln: 2.442 ± 0.199
2.617GlyArg: 2.617 ± 0.194
7.115GlySer: 7.115 ± 0.535
6.965GlyThr: 6.965 ± 0.563
5.769GlyVal: 5.769 ± 0.414
1.059GlyTrp: 1.059 ± 0.135
3.19GlyTyr: 3.19 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
0.86HisAla: 0.86 ± 0.097
0.212HisCys: 0.212 ± 0.062
1.022HisAsp: 1.022 ± 0.142
1.034HisGlu: 1.034 ± 0.132
0.822HisPhe: 0.822 ± 0.128
1.321HisGly: 1.321 ± 0.142
0.523HisHis: 0.523 ± 0.103
1.246HisIle: 1.246 ± 0.15
1.246HisLys: 1.246 ± 0.13
1.234HisLeu: 1.234 ± 0.14
0.312HisMet: 0.312 ± 0.067
1.084HisAsn: 1.084 ± 0.152
1.009HisPro: 1.009 ± 0.109
0.698HisGln: 0.698 ± 0.097
0.735HisArg: 0.735 ± 0.104
1.296HisSer: 1.296 ± 0.174
1.458HisThr: 1.458 ± 0.164
0.81HisVal: 0.81 ± 0.103
0.237HisTrp: 0.237 ± 0.059
1.146HisTyr: 1.146 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
3.913IleAla: 3.913 ± 0.221
0.635IleCys: 0.635 ± 0.094
4.847IleAsp: 4.847 ± 0.277
4.261IleGlu: 4.261 ± 0.247
2.156IlePhe: 2.156 ± 0.154
5.358IleGly: 5.358 ± 0.408
1.209IleHis: 1.209 ± 0.152
4.274IleIle: 4.274 ± 0.247
4.66IleLys: 4.66 ± 0.262
4.673IleLeu: 4.673 ± 0.267
1.084IleMet: 1.084 ± 0.127
4.237IleAsn: 4.237 ± 0.229
2.617IlePro: 2.617 ± 0.195
2.455IleGln: 2.455 ± 0.184
2.741IleArg: 2.741 ± 0.195
5.62IleSer: 5.62 ± 0.346
4.673IleThr: 4.673 ± 0.343
3.738IleVal: 3.738 ± 0.256
0.71IleTrp: 0.71 ± 0.107
2.243IleTyr: 2.243 ± 0.176
0.0IleXaa: 0.0 ± 0.0
Lys
3.452LysAla: 3.452 ± 0.241
0.623LysCys: 0.623 ± 0.097
4.361LysAsp: 4.361 ± 0.287
4.785LysGlu: 4.785 ± 0.408
2.866LysPhe: 2.866 ± 0.205
4.486LysGly: 4.486 ± 0.335
1.097LysHis: 1.097 ± 0.126
4.909LysIle: 4.909 ± 0.277
5.794LysLys: 5.794 ± 0.468
5.034LysLeu: 5.034 ± 0.344
1.657LysMet: 1.657 ± 0.196
3.8LysAsn: 3.8 ± 0.27
2.729LysPro: 2.729 ± 0.261
2.031LysGln: 2.031 ± 0.189
2.467LysArg: 2.467 ± 0.215
3.813LysSer: 3.813 ± 0.277
3.738LysThr: 3.738 ± 0.247
4.536LysVal: 4.536 ± 0.239
0.698LysTrp: 0.698 ± 0.091
2.966LysTyr: 2.966 ± 0.202
0.0LysXaa: 0.0 ± 0.0
Leu
3.888LeuAla: 3.888 ± 0.264
0.723LeuCys: 0.723 ± 0.102
5.271LeuAsp: 5.271 ± 0.255
4.436LeuGlu: 4.436 ± 0.263
2.716LeuPhe: 2.716 ± 0.2
4.934LeuGly: 4.934 ± 0.29
1.358LeuHis: 1.358 ± 0.162
4.199LeuIle: 4.199 ± 0.261
5.246LeuLys: 5.246 ± 0.321
4.212LeuLeu: 4.212 ± 0.289
1.408LeuMet: 1.408 ± 0.142
4.635LeuAsn: 4.635 ± 0.258
3.265LeuPro: 3.265 ± 0.243
2.529LeuGln: 2.529 ± 0.195
2.978LeuArg: 2.978 ± 0.216
5.009LeuSer: 5.009 ± 0.255
5.171LeuThr: 5.171 ± 0.325
4.249LeuVal: 4.249 ± 0.214
0.561LeuTrp: 0.561 ± 0.097
2.754LeuTyr: 2.754 ± 0.205
0.0LeuXaa: 0.0 ± 0.0
Met
1.57MetAla: 1.57 ± 0.17
0.237MetCys: 0.237 ± 0.061
1.221MetAsp: 1.221 ± 0.153
1.221MetGlu: 1.221 ± 0.152
0.86MetPhe: 0.86 ± 0.112
1.383MetGly: 1.383 ± 0.163
0.436MetHis: 0.436 ± 0.09
1.047MetIle: 1.047 ± 0.146
1.657MetLys: 1.657 ± 0.19
1.159MetLeu: 1.159 ± 0.145
0.548MetMet: 0.548 ± 0.093
1.234MetAsn: 1.234 ± 0.163
0.822MetPro: 0.822 ± 0.12
0.897MetGln: 0.897 ± 0.109
0.872MetArg: 0.872 ± 0.121
1.483MetSer: 1.483 ± 0.146
1.396MetThr: 1.396 ± 0.124
1.059MetVal: 1.059 ± 0.129
0.336MetTrp: 0.336 ± 0.068
0.673MetTyr: 0.673 ± 0.111
0.0MetXaa: 0.0 ± 0.0
Asn
3.501AsnAla: 3.501 ± 0.233
0.648AsnCys: 0.648 ± 0.086
3.701AsnAsp: 3.701 ± 0.261
3.09AsnGlu: 3.09 ± 0.188
2.741AsnPhe: 2.741 ± 0.209
4.486AsnGly: 4.486 ± 0.316
1.059AsnHis: 1.059 ± 0.136
4.448AsnIle: 4.448 ± 0.23
3.676AsnLys: 3.676 ± 0.272
4.723AsnLeu: 4.723 ± 0.283
0.897AsnMet: 0.897 ± 0.118
3.551AsnAsn: 3.551 ± 0.268
3.265AsnPro: 3.265 ± 0.201
1.956AsnGln: 1.956 ± 0.189
2.343AsnArg: 2.343 ± 0.174
4.286AsnSer: 4.286 ± 0.292
4.174AsnThr: 4.174 ± 0.307
4.635AsnVal: 4.635 ± 0.3
0.835AsnTrp: 0.835 ± 0.124
2.392AsnTyr: 2.392 ± 0.2
0.0AsnXaa: 0.0 ± 0.0
Pro
2.467ProAla: 2.467 ± 0.307
0.324ProCys: 0.324 ± 0.065
2.704ProAsp: 2.704 ± 0.212
2.642ProGlu: 2.642 ± 0.225
1.844ProPhe: 1.844 ± 0.165
3.813ProGly: 3.813 ± 0.464
0.997ProHis: 0.997 ± 0.113
2.293ProIle: 2.293 ± 0.172
2.654ProLys: 2.654 ± 0.24
2.43ProLeu: 2.43 ± 0.166
0.611ProMet: 0.611 ± 0.088
2.38ProAsn: 2.38 ± 0.157
2.168ProPro: 2.168 ± 0.342
1.308ProGln: 1.308 ± 0.118
1.67ProArg: 1.67 ± 0.15
3.713ProSer: 3.713 ± 0.305
3.626ProThr: 3.626 ± 0.367
2.691ProVal: 2.691 ± 0.219
0.461ProTrp: 0.461 ± 0.072
1.62ProTyr: 1.62 ± 0.155
0.0ProXaa: 0.0 ± 0.0
Gln
1.807GlnAla: 1.807 ± 0.14
0.386GlnCys: 0.386 ± 0.071
2.056GlnAsp: 2.056 ± 0.143
2.343GlnGlu: 2.343 ± 0.207
1.57GlnPhe: 1.57 ± 0.143
2.517GlnGly: 2.517 ± 0.193
0.598GlnHis: 0.598 ± 0.102
2.268GlnIle: 2.268 ± 0.153
2.181GlnLys: 2.181 ± 0.196
2.579GlnLeu: 2.579 ± 0.165
0.86GlnMet: 0.86 ± 0.118
1.794GlnAsn: 1.794 ± 0.156
1.022GlnPro: 1.022 ± 0.111
1.234GlnGln: 1.234 ± 0.118
1.121GlnArg: 1.121 ± 0.116
2.093GlnSer: 2.093 ± 0.137
2.218GlnThr: 2.218 ± 0.192
2.38GlnVal: 2.38 ± 0.172
0.399GlnTrp: 0.399 ± 0.074
1.956GlnTyr: 1.956 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
1.882ArgAla: 1.882 ± 0.148
0.449ArgCys: 0.449 ± 0.08
2.442ArgAsp: 2.442 ± 0.205
2.617ArgGlu: 2.617 ± 0.21
1.67ArgPhe: 1.67 ± 0.168
2.442ArgGly: 2.442 ± 0.172
0.586ArgHis: 0.586 ± 0.076
2.841ArgIle: 2.841 ± 0.2
2.729ArgLys: 2.729 ± 0.284
2.766ArgLeu: 2.766 ± 0.141
0.947ArgMet: 0.947 ± 0.124
2.006ArgAsn: 2.006 ± 0.153
0.972ArgPro: 0.972 ± 0.104
1.259ArgGln: 1.259 ± 0.142
1.707ArgArg: 1.707 ± 0.176
2.704ArgSer: 2.704 ± 0.183
2.068ArgThr: 2.068 ± 0.165
2.38ArgVal: 2.38 ± 0.205
0.399ArgTrp: 0.399 ± 0.072
1.869ArgTyr: 1.869 ± 0.173
0.0ArgXaa: 0.0 ± 0.0
Ser
4.436SerAla: 4.436 ± 0.262
0.623SerCys: 0.623 ± 0.098
4.324SerAsp: 4.324 ± 0.263
3.763SerGlu: 3.763 ± 0.261
3.202SerPhe: 3.202 ± 0.196
8.012SerGly: 8.012 ± 0.633
1.296SerHis: 1.296 ± 0.141
4.984SerIle: 4.984 ± 0.358
3.937SerLys: 3.937 ± 0.262
5.034SerLeu: 5.034 ± 0.301
1.396SerMet: 1.396 ± 0.137
4.909SerAsn: 4.909 ± 0.294
2.766SerPro: 2.766 ± 0.185
2.118SerGln: 2.118 ± 0.13
2.205SerArg: 2.205 ± 0.155
5.545SerSer: 5.545 ± 0.337
5.993SerThr: 5.993 ± 0.492
4.486SerVal: 4.486 ± 0.256
0.997SerTrp: 0.997 ± 0.117
3.04SerTyr: 3.04 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
4.735ThrAla: 4.735 ± 0.409
0.773ThrCys: 0.773 ± 0.12
4.124ThrAsp: 4.124 ± 0.287
3.888ThrGlu: 3.888 ± 0.23
3.227ThrPhe: 3.227 ± 0.326
7.539ThrGly: 7.539 ± 0.731
1.171ThrHis: 1.171 ± 0.128
4.947ThrIle: 4.947 ± 0.363
4.099ThrLys: 4.099 ± 0.26
5.022ThrLeu: 5.022 ± 0.266
1.022ThrMet: 1.022 ± 0.103
4.374ThrAsn: 4.374 ± 0.379
3.352ThrPro: 3.352 ± 0.219
2.492ThrGln: 2.492 ± 0.197
1.894ThrArg: 1.894 ± 0.153
5.408ThrSer: 5.408 ± 0.46
5.669ThrThr: 5.669 ± 0.699
4.747ThrVal: 4.747 ± 0.341
0.561ThrTrp: 0.561 ± 0.075
2.467ThrTyr: 2.467 ± 0.24
0.0ThrXaa: 0.0 ± 0.0
Val
4.261ValAla: 4.261 ± 0.319
0.748ValCys: 0.748 ± 0.114
4.81ValAsp: 4.81 ± 0.247
4.075ValGlu: 4.075 ± 0.236
2.143ValPhe: 2.143 ± 0.169
5.969ValGly: 5.969 ± 0.638
1.121ValHis: 1.121 ± 0.14
4.075ValIle: 4.075 ± 0.239
4.336ValLys: 4.336 ± 0.232
4.025ValLeu: 4.025 ± 0.212
1.333ValMet: 1.333 ± 0.135
4.099ValAsn: 4.099 ± 0.257
2.455ValPro: 2.455 ± 0.149
2.417ValGln: 2.417 ± 0.163
2.654ValArg: 2.654 ± 0.201
4.797ValSer: 4.797 ± 0.381
5.221ValThr: 5.221 ± 0.452
3.987ValVal: 3.987 ± 0.235
0.748ValTrp: 0.748 ± 0.105
2.48ValTyr: 2.48 ± 0.159
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.075
0.174TrpCys: 0.174 ± 0.053
0.897TrpAsp: 0.897 ± 0.12
0.785TrpGlu: 0.785 ± 0.111
0.498TrpPhe: 0.498 ± 0.084
0.959TrpGly: 0.959 ± 0.111
0.374TrpHis: 0.374 ± 0.073
0.748TrpIle: 0.748 ± 0.102
0.922TrpLys: 0.922 ± 0.122
0.635TrpLeu: 0.635 ± 0.106
0.411TrpMet: 0.411 ± 0.064
0.698TrpAsn: 0.698 ± 0.087
0.361TrpPro: 0.361 ± 0.07
0.399TrpGln: 0.399 ± 0.065
0.473TrpArg: 0.473 ± 0.1
0.822TrpSer: 0.822 ± 0.112
0.623TrpThr: 0.623 ± 0.088
0.76TrpVal: 0.76 ± 0.099
0.174TrpTrp: 0.174 ± 0.054
0.411TrpTyr: 0.411 ± 0.077
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.33TyrAla: 2.33 ± 0.178
0.673TyrCys: 0.673 ± 0.117
3.24TyrAsp: 3.24 ± 0.23
2.567TyrGlu: 2.567 ± 0.206
1.732TyrPhe: 1.732 ± 0.146
3.053TyrGly: 3.053 ± 0.217
0.86TyrHis: 0.86 ± 0.126
2.33TyrIle: 2.33 ± 0.214
2.442TyrLys: 2.442 ± 0.208
2.978TyrLeu: 2.978 ± 0.236
0.81TyrMet: 0.81 ± 0.113
2.966TyrAsn: 2.966 ± 0.227
1.819TyrPro: 1.819 ± 0.186
1.545TyrGln: 1.545 ± 0.147
1.62TyrArg: 1.62 ± 0.141
2.991TyrSer: 2.991 ± 0.197
2.529TyrThr: 2.529 ± 0.173
2.791TyrVal: 2.791 ± 0.225
0.399TyrTrp: 0.399 ± 0.082
2.143TyrTyr: 2.143 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 334 proteins (80255 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski