Actinobacteria bacterium OK074

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; unclassified Actinomycetia

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1FUN8|A0A0N1FUN8_9ACTN Peptidase M20 OS=Actinobacteria bacterium OK074 OX=1592327 GN=OK074_4172 PE=4 SV=1
MM1 pKa = 7.66PAHH4 pKa = 5.64PTGRR8 pKa = 11.84RR9 pKa = 11.84AALLMSALTLTLGTVLAAPIALAGTAVAATPEE41 pKa = 3.82ATAALGNGGSQLFYY55 pKa = 10.39TAAAGQANKK64 pKa = 8.45VTVTVSPGGDD74 pKa = 3.14TDD76 pKa = 4.17LSYY79 pKa = 11.43VIDD82 pKa = 4.34DD83 pKa = 4.2VVPIAAGDD91 pKa = 4.07GCTHH95 pKa = 7.52PDD97 pKa = 3.12SADD100 pKa = 3.37ATKK103 pKa = 10.55VACTVPEE110 pKa = 5.0LDD112 pKa = 3.69SQDD115 pKa = 3.48PYY117 pKa = 12.08AVFEE121 pKa = 4.75ADD123 pKa = 5.17LGDD126 pKa = 4.2GNDD129 pKa = 4.67GITLVDD135 pKa = 3.83SSDD138 pKa = 3.06QVYY141 pKa = 10.27YY142 pKa = 10.84YY143 pKa = 11.33NEE145 pKa = 4.19VNLGTGNDD153 pKa = 3.6SYY155 pKa = 12.02VNTGRR160 pKa = 11.84LDD162 pKa = 3.43GSLVRR167 pKa = 11.84GQAGADD173 pKa = 3.54TITAGEE179 pKa = 4.04AAIVLAGDD187 pKa = 4.57DD188 pKa = 4.13NDD190 pKa = 4.43SVTANGNYY198 pKa = 10.11VIVDD202 pKa = 3.83GGKK205 pKa = 10.81GNDD208 pKa = 3.78VIHH211 pKa = 6.83GGDD214 pKa = 3.44KK215 pKa = 10.82GQSLSGGDD223 pKa = 4.32GNDD226 pKa = 3.12TLYY229 pKa = 11.07GGKK232 pKa = 10.68GNDD235 pKa = 3.13SLYY238 pKa = 10.84GGKK241 pKa = 10.74GNDD244 pKa = 3.57VLWGNSGNDD253 pKa = 3.62VLWGNSGNDD262 pKa = 3.45KK263 pKa = 10.91LYY265 pKa = 10.94GGPGTDD271 pKa = 3.66RR272 pKa = 11.84LSGGPGTDD280 pKa = 3.06VTQGG284 pKa = 3.04

Molecular weight: 28.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1G0Z6|A0A0N1G0Z6_9ACTN Alcohol dehydrogenase GroES domain protein OS=Actinobacteria bacterium OK074 OX=1592327 GN=OK074_5661 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.88GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9137

0

9137

3121415

30

7934

341.6

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.691 ± 0.041

0.768 ± 0.007

6.013 ± 0.023

5.464 ± 0.028

2.713 ± 0.014

9.531 ± 0.026

2.32 ± 0.013

2.981 ± 0.02

2.029 ± 0.019

10.315 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.619 ± 0.011

1.796 ± 0.013

6.222 ± 0.025

2.732 ± 0.015

7.935 ± 0.03

5.125 ± 0.025

6.532 ± 0.033

8.604 ± 0.025

1.491 ± 0.01

2.12 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski