Amino acid dipepetide frequency for Actinobacteria bacterium OK074

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.597AlaAla: 21.597 ± 0.137	1.073AlaCys: 1.073 ± 0.02	8.702AlaAsp: 8.702 ± 0.064	8.657AlaGlu: 8.657 ± 0.076	3.5AlaPhe: 3.5 ± 0.038	12.899AlaGly: 12.899 ± 0.085	3.018AlaHis: 3.018 ± 0.034	3.227AlaIle: 3.227 ± 0.04	2.755AlaLys: 2.755 ± 0.044	14.55AlaLeu: 14.55 ± 0.1	2.311AlaMet: 2.311 ± 0.028	2.071AlaAsn: 2.071 ± 0.026	7.304AlaPro: 7.304 ± 0.057	3.809AlaGln: 3.809 ± 0.042	10.215AlaArg: 10.215 ± 0.072	5.893AlaSer: 5.893 ± 0.052	7.699AlaThr: 7.699 ± 0.064	12.981AlaVal: 12.981 ± 0.099	1.814AlaTrp: 1.814 ± 0.025	2.839AlaTyr: 2.839 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.087CysAla: 1.087 ± 0.018	0.08CysCys: 0.08 ± 0.005	0.484CysAsp: 0.484 ± 0.012	0.38CysGlu: 0.38 ± 0.013	0.216CysPhe: 0.216 ± 0.01	0.908CysGly: 0.908 ± 0.018	0.191CysHis: 0.191 ± 0.007	0.151CysIle: 0.151 ± 0.007	0.112CysLys: 0.112 ± 0.007	0.743CysLeu: 0.743 ± 0.016	0.097CysMet: 0.097 ± 0.006	0.109CysAsn: 0.109 ± 0.006	0.471CysPro: 0.471 ± 0.012	0.161CysGln: 0.161 ± 0.006	0.606CysArg: 0.606 ± 0.014	0.415CysSer: 0.415 ± 0.012	0.547CysThr: 0.547 ± 0.014	0.657CysVal: 0.657 ± 0.016	0.117CysTrp: 0.117 ± 0.006	0.155CysTyr: 0.155 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.684AspAla: 7.684 ± 0.064	0.416AspCys: 0.416 ± 0.013	3.746AspAsp: 3.746 ± 0.045	3.622AspGlu: 3.622 ± 0.04	1.603AspPhe: 1.603 ± 0.023	6.618AspGly: 6.618 ± 0.057	1.442AspHis: 1.442 ± 0.024	1.849AspIle: 1.849 ± 0.026	1.098AspLys: 1.098 ± 0.023	6.18AspLeu: 6.18 ± 0.056	0.754AspMet: 0.754 ± 0.016	1.011AspAsn: 1.011 ± 0.021	4.549AspPro: 4.549 ± 0.044	1.515AspGln: 1.515 ± 0.027	4.887AspArg: 4.887 ± 0.045	2.658AspSer: 2.658 ± 0.036	3.561AspThr: 3.561 ± 0.04	4.816AspVal: 4.816 ± 0.039	1.007AspTrp: 1.007 ± 0.018	1.114AspTyr: 1.114 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.943GluAla: 6.943 ± 0.066	0.348GluCys: 0.348 ± 0.01	2.643GluAsp: 2.643 ± 0.036	3.181GluGlu: 3.181 ± 0.041	1.519GluPhe: 1.519 ± 0.026	4.092GluGly: 4.092 ± 0.037	1.545GluHis: 1.545 ± 0.026	2.134GluIle: 2.134 ± 0.028	1.21GluLys: 1.21 ± 0.023	6.854GluLeu: 6.854 ± 0.061	0.767GluMet: 0.767 ± 0.016	0.973GluAsn: 0.973 ± 0.018	3.367GluPro: 3.367 ± 0.038	2.147GluGln: 2.147 ± 0.029	5.264GluArg: 5.264 ± 0.05	2.572GluSer: 2.572 ± 0.029	2.988GluThr: 2.988 ± 0.031	4.228GluVal: 4.228 ± 0.042	0.748GluTrp: 0.748 ± 0.017	1.115GluTyr: 1.115 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.717PheAla: 3.717 ± 0.036	0.24PheCys: 0.24 ± 0.008	1.959PheAsp: 1.959 ± 0.029	1.401PheGlu: 1.401 ± 0.023	0.861PhePhe: 0.861 ± 0.018	3.052PheGly: 3.052 ± 0.036	0.608PheHis: 0.608 ± 0.015	0.634PheIle: 0.634 ± 0.017	0.492PheLys: 0.492 ± 0.016	2.574PheLeu: 2.574 ± 0.033	0.381PheMet: 0.381 ± 0.011	0.538PheAsn: 0.538 ± 0.011	1.41PhePro: 1.41 ± 0.025	0.676PheGln: 0.676 ± 0.017	1.824PheArg: 1.824 ± 0.023	1.419PheSer: 1.419 ± 0.021	2.063PheThr: 2.063 ± 0.028	2.297PheVal: 2.297 ± 0.03	0.411PheTrp: 0.411 ± 0.012	0.574PheTyr: 0.574 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.383GlyAla: 11.383 ± 0.072	0.816GlyCys: 0.816 ± 0.016	5.098GlyAsp: 5.098 ± 0.047	4.945GlyGlu: 4.945 ± 0.049	2.854GlyPhe: 2.854 ± 0.037	9.166GlyGly: 9.166 ± 0.085	2.377GlyHis: 2.377 ± 0.03	3.424GlyIle: 3.424 ± 0.039	2.226GlyLys: 2.226 ± 0.035	9.369GlyLeu: 9.369 ± 0.07	1.949GlyMet: 1.949 ± 0.028	1.762GlyAsn: 1.762 ± 0.029	5.271GlyPro: 5.271 ± 0.049	2.731GlyGln: 2.731 ± 0.032	7.773GlyArg: 7.773 ± 0.058	5.567GlySer: 5.567 ± 0.056	6.9GlyThr: 6.9 ± 0.052	7.733GlyVal: 7.733 ± 0.074	1.677GlyTrp: 1.677 ± 0.027	2.285GlyTyr: 2.285 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	2.776HisAla: 2.776 ± 0.033	0.208HisCys: 0.208 ± 0.009	1.339HisAsp: 1.339 ± 0.023	1.212HisGlu: 1.212 ± 0.021	0.661HisPhe: 0.661 ± 0.014	2.406HisGly: 2.406 ± 0.028	0.716HisHis: 0.716 ± 0.016	0.681HisIle: 0.681 ± 0.017	0.337HisLys: 0.337 ± 0.011	2.438HisLeu: 2.438 ± 0.028	0.296HisMet: 0.296 ± 0.009	0.397HisAsn: 0.397 ± 0.011	1.891HisPro: 1.891 ± 0.028	0.668HisGln: 0.668 ± 0.017	2.136HisArg: 2.136 ± 0.032	1.007HisSer: 1.007 ± 0.02	1.437HisThr: 1.437 ± 0.022	1.752HisVal: 1.752 ± 0.023	0.35HisTrp: 0.35 ± 0.011	0.49HisTyr: 0.49 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.52IleAla: 4.52 ± 0.045	0.268IleCys: 0.268 ± 0.01	2.048IleAsp: 2.048 ± 0.031	1.786IleGlu: 1.786 ± 0.024	0.614IlePhe: 0.614 ± 0.014	3.335IleGly: 3.335 ± 0.04	0.59IleHis: 0.59 ± 0.014	0.753IleIle: 0.753 ± 0.018	0.667IleLys: 0.667 ± 0.017	2.353IleLeu: 2.353 ± 0.034	0.4IleMet: 0.4 ± 0.012	0.641IleAsn: 0.641 ± 0.016	1.766IlePro: 1.766 ± 0.025	0.705IleGln: 0.705 ± 0.017	2.171IleArg: 2.171 ± 0.028	1.611IleSer: 1.611 ± 0.026	2.132IleThr: 2.132 ± 0.029	2.605IleVal: 2.605 ± 0.033	0.341IleTrp: 0.341 ± 0.011	0.502IleTyr: 0.502 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.762LysAla: 2.762 ± 0.042	0.101LysCys: 0.101 ± 0.006	1.223LysAsp: 1.223 ± 0.025	1.076LysGlu: 1.076 ± 0.023	0.432LysPhe: 0.432 ± 0.011	1.739LysGly: 1.739 ± 0.033	0.381LysHis: 0.381 ± 0.013	0.778LysIle: 0.778 ± 0.019	0.811LysLys: 0.811 ± 0.027	1.886LysLeu: 1.886 ± 0.028	0.356LysMet: 0.356 ± 0.01	0.516LysAsn: 0.516 ± 0.016	1.25LysPro: 1.25 ± 0.024	0.638LysGln: 0.638 ± 0.016	1.319LysArg: 1.319 ± 0.023	1.173LysSer: 1.173 ± 0.023	1.292LysThr: 1.292 ± 0.027	1.831LysVal: 1.831 ± 0.031	0.256LysTrp: 0.256 ± 0.01	0.462LysTyr: 0.462 ± 0.014	0.0LysXaa: 0.0 ± 0.0
Leu	15.281LeuAla: 15.281 ± 0.11	0.835LeuCys: 0.835 ± 0.019	6.77LeuAsp: 6.77 ± 0.055	4.482LeuGlu: 4.482 ± 0.048	2.647LeuPhe: 2.647 ± 0.036	9.347LeuGly: 9.347 ± 0.053	2.349LeuHis: 2.349 ± 0.031	2.962LeuIle: 2.962 ± 0.032	1.981LeuLys: 1.981 ± 0.028	11.277LeuLeu: 11.277 ± 0.087	1.532LeuMet: 1.532 ± 0.021	1.775LeuAsn: 1.775 ± 0.026	6.731LeuPro: 6.731 ± 0.053	2.07LeuGln: 2.07 ± 0.03	8.586LeuArg: 8.586 ± 0.065	5.284LeuSer: 5.284 ± 0.045	7.153LeuThr: 7.153 ± 0.057	8.847LeuVal: 8.847 ± 0.068	1.347LeuTrp: 1.347 ± 0.024	1.898LeuTyr: 1.898 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.121MetAla: 2.121 ± 0.029	0.127MetCys: 0.127 ± 0.007	0.803MetAsp: 0.803 ± 0.016	0.673MetGlu: 0.673 ± 0.016	0.426MetPhe: 0.426 ± 0.011	1.231MetGly: 1.231 ± 0.021	0.331MetHis: 0.331 ± 0.01	0.586MetIle: 0.586 ± 0.013	0.379MetLys: 0.379 ± 0.012	1.532MetLeu: 1.532 ± 0.023	0.274MetMet: 0.274 ± 0.011	0.411MetAsn: 0.411 ± 0.012	1.046MetPro: 1.046 ± 0.018	0.4MetGln: 0.4 ± 0.013	1.322MetArg: 1.322 ± 0.021	1.32MetSer: 1.32 ± 0.02	1.49MetThr: 1.49 ± 0.024	1.209MetVal: 1.209 ± 0.023	0.194MetTrp: 0.194 ± 0.008	0.309MetTyr: 0.309 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.232AsnAla: 2.232 ± 0.028	0.152AsnCys: 0.152 ± 0.007	0.984AsnAsp: 0.984 ± 0.019	0.786AsnGlu: 0.786 ± 0.018	0.469AsnPhe: 0.469 ± 0.012	1.959AsnGly: 1.959 ± 0.028	0.391AsnHis: 0.391 ± 0.011	0.645AsnIle: 0.645 ± 0.016	0.378AsnLys: 0.378 ± 0.012	1.635AsnLeu: 1.635 ± 0.024	0.289AsnMet: 0.289 ± 0.01	0.432AsnAsn: 0.432 ± 0.012	1.386AsnPro: 1.386 ± 0.024	0.514AsnGln: 0.514 ± 0.013	1.274AsnArg: 1.274 ± 0.021	1.091AsnSer: 1.091 ± 0.02	1.244AsnThr: 1.244 ± 0.023	1.373AsnVal: 1.373 ± 0.026	0.286AsnTrp: 0.286 ± 0.011	0.435AsnTyr: 0.435 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.781ProAla: 8.781 ± 0.067	0.325ProCys: 0.325 ± 0.011	4.622ProAsp: 4.622 ± 0.048	4.304ProGlu: 4.304 ± 0.043	1.543ProPhe: 1.543 ± 0.025	6.777ProGly: 6.777 ± 0.06	1.449ProHis: 1.449 ± 0.023	1.223ProIle: 1.223 ± 0.019	1.169ProLys: 1.169 ± 0.021	5.503ProLeu: 5.503 ± 0.05	0.959ProMet: 0.959 ± 0.02	0.978ProAsn: 0.978 ± 0.017	3.883ProPro: 3.883 ± 0.057	1.721ProGln: 1.721 ± 0.029	4.103ProArg: 4.103 ± 0.056	3.257ProSer: 3.257 ± 0.042	3.54ProThr: 3.54 ± 0.045	5.741ProVal: 5.741 ± 0.048	0.874ProTrp: 0.874 ± 0.016	1.47ProTyr: 1.47 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.553GlnAla: 3.553 ± 0.038	0.175GlnCys: 0.175 ± 0.007	1.437GlnAsp: 1.437 ± 0.023	1.395GlnGlu: 1.395 ± 0.022	0.679GlnPhe: 0.679 ± 0.016	2.26GlnGly: 2.26 ± 0.032	0.662GlnHis: 0.662 ± 0.013	0.995GlnIle: 0.995 ± 0.02	0.568GlnLys: 0.568 ± 0.015	3.275GlnLeu: 3.275 ± 0.036	0.425GlnMet: 0.425 ± 0.012	0.491GlnAsn: 0.491 ± 0.015	1.718GlnPro: 1.718 ± 0.027	1.226GlnGln: 1.226 ± 0.026	2.327GlnArg: 2.327 ± 0.033	1.318GlnSer: 1.318 ± 0.021	1.394GlnThr: 1.394 ± 0.025	2.326GlnVal: 2.326 ± 0.032	0.459GlnTrp: 0.459 ± 0.015	0.63GlnTyr: 0.63 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	9.972ArgAla: 9.972 ± 0.078	0.591ArgCys: 0.591 ± 0.015	4.135ArgAsp: 4.135 ± 0.037	4.62ArgGlu: 4.62 ± 0.047	2.303ArgPhe: 2.303 ± 0.029	5.858ArgGly: 5.858 ± 0.045	2.157ArgHis: 2.157 ± 0.03	3.098ArgIle: 3.098 ± 0.037	1.483ArgLys: 1.483 ± 0.024	8.766ArgLeu: 8.766 ± 0.06	1.611ArgMet: 1.611 ± 0.023	1.265ArgAsn: 1.265 ± 0.021	5.114ArgPro: 5.114 ± 0.06	2.362ArgGln: 2.362 ± 0.027	7.573ArgArg: 7.573 ± 0.071	3.826ArgSer: 3.826 ± 0.034	5.649ArgThr: 5.649 ± 0.045	5.91ArgVal: 5.91 ± 0.051	1.331ArgTrp: 1.331 ± 0.022	1.728ArgTyr: 1.728 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.982SerAla: 6.982 ± 0.06	0.416SerCys: 0.416 ± 0.012	2.761SerAsp: 2.761 ± 0.034	2.208SerGlu: 2.208 ± 0.028	1.501SerPhe: 1.501 ± 0.025	6.25SerGly: 6.25 ± 0.058	0.981SerHis: 0.981 ± 0.018	1.357SerIle: 1.357 ± 0.02	0.992SerLys: 0.992 ± 0.021	4.884SerLeu: 4.884 ± 0.043	1.006SerMet: 1.006 ± 0.017	0.928SerAsn: 0.928 ± 0.018	3.242SerPro: 3.242 ± 0.039	1.219SerGln: 1.219 ± 0.023	3.527SerArg: 3.527 ± 0.033	3.154SerSer: 3.154 ± 0.047	3.377SerThr: 3.377 ± 0.045	4.224SerVal: 4.224 ± 0.039	0.921SerTrp: 0.921 ± 0.018	1.32SerTyr: 1.32 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	10.123ThrAla: 10.123 ± 0.08	0.435ThrCys: 0.435 ± 0.013	3.85ThrAsp: 3.85 ± 0.041	3.284ThrGlu: 3.284 ± 0.034	1.653ThrPhe: 1.653 ± 0.024	7.157ThrGly: 7.157 ± 0.059	1.235ThrHis: 1.235 ± 0.022	1.717ThrIle: 1.717 ± 0.031	1.24ThrLys: 1.24 ± 0.03	5.918ThrLeu: 5.918 ± 0.048	0.905ThrMet: 0.905 ± 0.017	1.067ThrAsn: 1.067 ± 0.022	4.524ThrPro: 4.524 ± 0.047	1.385ThrGln: 1.385 ± 0.021	3.991ThrArg: 3.991 ± 0.038	3.43ThrSer: 3.43 ± 0.044	4.601ThrThr: 4.601 ± 0.077	6.401ThrVal: 6.401 ± 0.052	0.927ThrTrp: 0.927 ± 0.019	1.473ThrTyr: 1.473 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.908ValAla: 10.908 ± 0.084	0.75ValCys: 0.75 ± 0.017	5.093ValAsp: 5.093 ± 0.045	4.68ValGlu: 4.68 ± 0.043	2.518ValPhe: 2.518 ± 0.028	6.766ValGly: 6.766 ± 0.054	1.995ValHis: 1.995 ± 0.026	2.618ValIle: 2.618 ± 0.034	1.657ValLys: 1.657 ± 0.029	9.585ValLeu: 9.585 ± 0.07	1.37ValMet: 1.37 ± 0.02	1.762ValAsn: 1.762 ± 0.027	5.416ValPro: 5.416 ± 0.054	2.163ValGln: 2.163 ± 0.026	7.405ValArg: 7.405 ± 0.061	4.346ValSer: 4.346 ± 0.043	5.889ValThr: 5.889 ± 0.049	8.415ValVal: 8.415 ± 0.071	1.148ValTrp: 1.148 ± 0.02	1.554ValTyr: 1.554 ± 0.023	0.0ValXaa: 0.0 ± 0.0
Trp	1.617TrpAla: 1.617 ± 0.024	0.15TrpCys: 0.15 ± 0.007	0.813TrpAsp: 0.813 ± 0.017	0.71TrpGlu: 0.71 ± 0.015	0.486TrpPhe: 0.486 ± 0.013	1.088TrpGly: 1.088 ± 0.019	0.39TrpHis: 0.39 ± 0.012	0.522TrpIle: 0.522 ± 0.016	0.34TrpLys: 0.34 ± 0.012	1.747TrpLeu: 1.747 ± 0.027	0.273TrpMet: 0.273 ± 0.01	0.397TrpAsn: 0.397 ± 0.013	0.788TrpPro: 0.788 ± 0.017	0.619TrpGln: 0.619 ± 0.015	1.279TrpArg: 1.279 ± 0.021	0.951TrpSer: 0.951 ± 0.019	1.111TrpThr: 1.111 ± 0.02	0.938TrpVal: 0.938 ± 0.019	0.322TrpTrp: 0.322 ± 0.011	0.37TrpTyr: 0.37 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.876TyrAla: 2.876 ± 0.034	0.18TyrCys: 0.18 ± 0.007	1.622TyrAsp: 1.622 ± 0.032	1.234TyrGlu: 1.234 ± 0.02	0.646TyrPhe: 0.646 ± 0.016	2.4TyrGly: 2.4 ± 0.03	0.39TyrHis: 0.39 ± 0.012	0.453TyrIle: 0.453 ± 0.013	0.388TyrLys: 0.388 ± 0.014	2.084TyrLeu: 2.084 ± 0.032	0.23TyrMet: 0.23 ± 0.008	0.429TyrAsn: 0.429 ± 0.013	1.093TyrPro: 1.093 ± 0.019	0.586TyrGln: 0.586 ± 0.016	1.771TyrArg: 1.771 ± 0.03	0.959TyrSer: 0.959 ± 0.019	1.25TyrThr: 1.25 ± 0.022	1.757TyrVal: 1.757 ± 0.024	0.378TyrTrp: 0.378 ± 0.012	0.474TyrTyr: 0.474 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9137 proteins (3121415 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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