Setaria italica (Foxtail millet) (Panicum italicum)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; PACMAD clade; Panicoideae; Panicodae; Paniceae;

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39467 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K3XFV8|K3XFV8_SETIT Aspartate--tRNA ligase OS=Setaria italica OX=4555 PE=3 SV=1
MM1 pKa = 7.81PDD3 pKa = 2.6WRR5 pKa = 11.84VGEE8 pKa = 4.22FEE10 pKa = 5.44GKK12 pKa = 8.49FTDD15 pKa = 5.31EE16 pKa = 4.27FAQSNRR22 pKa = 11.84SEE24 pKa = 4.16HH25 pKa = 6.04EE26 pKa = 4.26SGIEE30 pKa = 3.92APDD33 pKa = 3.44VTSSKK38 pKa = 10.47KK39 pKa = 10.18LKK41 pKa = 10.12HH42 pKa = 5.74AAASEE47 pKa = 4.49KK48 pKa = 9.84IHH50 pKa = 6.27QGVISGTNDD59 pKa = 3.28SDD61 pKa = 3.97SQKK64 pKa = 10.72CNSEE68 pKa = 4.68HH69 pKa = 5.78IQCANGNINSNGDD82 pKa = 3.71CKK84 pKa = 10.95DD85 pKa = 3.06GSNAFTSRR93 pKa = 11.84EE94 pKa = 3.84EE95 pKa = 3.87NTIVEE100 pKa = 4.71TRR102 pKa = 11.84CPTDD106 pKa = 2.59NWNSCQFALSNGSSILNNHH125 pKa = 6.3SAPQDD130 pKa = 3.42SLAYY134 pKa = 10.25GDD136 pKa = 4.7NDD138 pKa = 4.25LNYY141 pKa = 9.66IDD143 pKa = 5.09WPGIDD148 pKa = 3.64NFEE151 pKa = 4.89DD152 pKa = 3.42VDD154 pKa = 4.03TLFRR158 pKa = 11.84RR159 pKa = 11.84SDD161 pKa = 3.22STYY164 pKa = 10.89GQQQLEE170 pKa = 4.4NTDD173 pKa = 3.54GLSWIPSSSDD183 pKa = 2.81AVYY186 pKa = 10.86SSDD189 pKa = 3.78VALQQGFGSSYY200 pKa = 10.8SDD202 pKa = 3.52YY203 pKa = 11.45GILDD207 pKa = 4.22DD208 pKa = 6.54LSAFQCAEE216 pKa = 4.21DD217 pKa = 3.98KK218 pKa = 10.95SLPSVDD224 pKa = 4.39PSAALCDD231 pKa = 3.64NQFDD235 pKa = 3.98DD236 pKa = 4.4TYY238 pKa = 10.95MFSEE242 pKa = 4.15QKK244 pKa = 10.46NVNGYY249 pKa = 10.31GDD251 pKa = 3.77QIYY254 pKa = 10.02QEE256 pKa = 5.07DD257 pKa = 4.42VMEE260 pKa = 5.44LLPTDD265 pKa = 4.08QICNGNGNIDD275 pKa = 3.42MM276 pKa = 5.44

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K3Y448|K3Y448_SETIT Uncharacterized protein OS=Setaria italica OX=4555 PE=4 SV=1
MM1 pKa = 7.33RR2 pKa = 11.84CRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84GKK13 pKa = 10.15CSGGRR18 pKa = 11.84RR19 pKa = 11.84GTRR22 pKa = 11.84ARR24 pKa = 11.84GQRR27 pKa = 11.84GTPPTGARR35 pKa = 11.84SRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GPRR43 pKa = 11.84LWDD46 pKa = 3.09ARR48 pKa = 11.84MGDD51 pKa = 3.43GTPRR55 pKa = 11.84TRR57 pKa = 11.84RR58 pKa = 11.84LPRR61 pKa = 11.84RR62 pKa = 11.84ARR64 pKa = 11.84RR65 pKa = 11.84GRR67 pKa = 11.84WRR69 pKa = 11.84NGGTSGRR76 pKa = 11.84TRR78 pKa = 11.84SPPPQSRR85 pKa = 11.84NRR87 pKa = 11.84HH88 pKa = 3.73GRR90 pKa = 11.84TRR92 pKa = 11.84RR93 pKa = 11.84GKK95 pKa = 10.57ASGPLL100 pKa = 3.39

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35701

3766

39467

14674120

8

5181

371.8

40.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.269 ± 0.021

1.948 ± 0.006

5.367 ± 0.011

5.979 ± 0.014

3.718 ± 0.008

7.295 ± 0.013

2.494 ± 0.006

4.41 ± 0.01

4.918 ± 0.013

9.598 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.006

3.509 ± 0.009

5.65 ± 0.015

3.452 ± 0.008

6.323 ± 0.012

8.153 ± 0.015

4.762 ± 0.008

6.769 ± 0.01

1.331 ± 0.004

2.63 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski