Amino acid dipepetide frequency for Mannheimia varigena USDA-ARS-USMARC-1296

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.764AlaAla: 6.764 ± 0.165
1.12AlaCys: 1.12 ± 0.041
4.418AlaAsp: 4.418 ± 0.092
6.545AlaGlu: 6.545 ± 0.137
3.68AlaPhe: 3.68 ± 0.086
5.701AlaGly: 5.701 ± 0.127
1.432AlaHis: 1.432 ± 0.049
6.486AlaIle: 6.486 ± 0.12
6.419AlaLys: 6.419 ± 0.127
9.216AlaLeu: 9.216 ± 0.135
2.281AlaMet: 2.281 ± 0.071
4.029AlaAsn: 4.029 ± 0.115
2.479AlaPro: 2.479 ± 0.07
3.617AlaGln: 3.617 ± 0.075
3.458AlaArg: 3.458 ± 0.083
4.137AlaSer: 4.137 ± 0.096
4.563AlaThr: 4.563 ± 0.13
6.449AlaVal: 6.449 ± 0.112
0.844AlaTrp: 0.844 ± 0.039
2.38AlaTyr: 2.38 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.682CysAla: 0.682 ± 0.036
0.132CysCys: 0.132 ± 0.014
0.549CysAsp: 0.549 ± 0.028
0.691CysGlu: 0.691 ± 0.037
0.468CysPhe: 0.468 ± 0.028
0.934CysGly: 0.934 ± 0.045
0.265CysHis: 0.265 ± 0.023
0.535CysIle: 0.535 ± 0.03
0.519CysLys: 0.519 ± 0.032
1.018CysLeu: 1.018 ± 0.043
0.153CysMet: 0.153 ± 0.016
0.375CysAsn: 0.375 ± 0.028
0.435CysPro: 0.435 ± 0.028
0.39CysGln: 0.39 ± 0.026
0.414CysArg: 0.414 ± 0.027
0.684CysSer: 0.684 ± 0.036
0.49CysThr: 0.49 ± 0.029
0.645CysVal: 0.645 ± 0.031
0.123CysTrp: 0.123 ± 0.013
0.367CysTyr: 0.367 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
3.452AspAla: 3.452 ± 0.085
0.507AspCys: 0.507 ± 0.026
2.4AspAsp: 2.4 ± 0.074
3.728AspGlu: 3.728 ± 0.085
2.629AspPhe: 2.629 ± 0.067
3.371AspGly: 3.371 ± 0.221
0.862AspHis: 0.862 ± 0.04
3.584AspIle: 3.584 ± 0.083
3.5AspLys: 3.5 ± 0.084
5.142AspLeu: 5.142 ± 0.105
1.145AspMet: 1.145 ± 0.044
2.341AspAsn: 2.341 ± 0.071
1.988AspPro: 1.988 ± 0.064
1.402AspGln: 1.402 ± 0.045
2.043AspArg: 2.043 ± 0.055
2.494AspSer: 2.494 ± 0.063
2.183AspThr: 2.183 ± 0.073
3.502AspVal: 3.502 ± 0.074
0.732AspTrp: 0.732 ± 0.036
2.082AspTyr: 2.082 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
5.166GluAla: 5.166 ± 0.105
0.514GluCys: 0.514 ± 0.025
2.712GluAsp: 2.712 ± 0.069
4.313GluGlu: 4.313 ± 0.097
2.604GluPhe: 2.604 ± 0.07
3.559GluGly: 3.559 ± 0.075
1.405GluHis: 1.405 ± 0.05
4.848GluIle: 4.848 ± 0.102
5.444GluLys: 5.444 ± 0.109
6.942GluLeu: 6.942 ± 0.126
1.989GluMet: 1.989 ± 0.06
3.945GluAsn: 3.945 ± 0.078
2.09GluPro: 2.09 ± 0.054
4.19GluGln: 4.19 ± 0.087
3.407GluArg: 3.407 ± 0.079
3.142GluSer: 3.142 ± 0.064
3.209GluThr: 3.209 ± 0.075
4.254GluVal: 4.254 ± 0.092
0.841GluTrp: 0.841 ± 0.036
1.787GluTyr: 1.787 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
4.094PheAla: 4.094 ± 0.086
0.606PheCys: 0.606 ± 0.028
2.815PheAsp: 2.815 ± 0.072
2.823PheGlu: 2.823 ± 0.072
2.091PhePhe: 2.091 ± 0.066
3.572PheGly: 3.572 ± 0.08
0.823PheHis: 0.823 ± 0.035
3.272PheIle: 3.272 ± 0.085
2.255PheLys: 2.255 ± 0.057
3.996PheLeu: 3.996 ± 0.101
1.039PheMet: 1.039 ± 0.039
2.365PheAsn: 2.365 ± 0.068
1.526PhePro: 1.526 ± 0.049
1.391PheGln: 1.391 ± 0.046
1.492PheArg: 1.492 ± 0.044
3.293PheSer: 3.293 ± 0.078
2.341PheThr: 2.341 ± 0.062
3.065PheVal: 3.065 ± 0.059
0.534PheTrp: 0.534 ± 0.028
1.571PheTyr: 1.571 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
5.079GlyAla: 5.079 ± 0.118
0.836GlyCys: 0.836 ± 0.041
3.215GlyAsp: 3.215 ± 0.101
4.457GlyGlu: 4.457 ± 0.094
3.23GlyPhe: 3.23 ± 0.067
4.897GlyGly: 4.897 ± 0.122
1.207GlyHis: 1.207 ± 0.052
5.304GlyIle: 5.304 ± 0.096
5.284GlyLys: 5.284 ± 0.139
6.973GlyLeu: 6.973 ± 0.11
1.953GlyMet: 1.953 ± 0.06
2.959GlyAsn: 2.959 ± 0.095
1.121GlyPro: 1.121 ± 0.049
2.424GlyGln: 2.424 ± 0.07
2.778GlyArg: 2.778 ± 0.069
4.044GlySer: 4.044 ± 0.101
3.523GlyThr: 3.523 ± 0.105
5.333GlyVal: 5.333 ± 0.092
0.881GlyTrp: 0.881 ± 0.034
2.605GlyTyr: 2.605 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.21HisAla: 1.21 ± 0.045
0.324HisCys: 0.324 ± 0.022
0.803HisAsp: 0.803 ± 0.036
0.818HisGlu: 0.818 ± 0.038
1.201HisPhe: 1.201 ± 0.036
1.273HisGly: 1.273 ± 0.044
0.6HisHis: 0.6 ± 0.035
1.586HisIle: 1.586 ± 0.05
1.135HisLys: 1.135 ± 0.043
2.193HisLeu: 2.193 ± 0.06
0.322HisMet: 0.322 ± 0.021
1.003HisAsn: 1.003 ± 0.037
0.941HisPro: 0.941 ± 0.036
1.051HisGln: 1.051 ± 0.036
0.958HisArg: 0.958 ± 0.041
1.349HisSer: 1.349 ± 0.044
1.015HisThr: 1.015 ± 0.037
0.708HisVal: 0.708 ± 0.034
0.273HisTrp: 0.273 ± 0.02
0.862HisTyr: 0.862 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
7.06IleAla: 7.06 ± 0.128
0.82IleCys: 0.82 ± 0.035
3.778IleAsp: 3.778 ± 0.082
4.816IleGlu: 4.816 ± 0.093
3.056IlePhe: 3.056 ± 0.077
5.402IleGly: 5.402 ± 0.105
1.282IleHis: 1.282 ± 0.041
4.527IleIle: 4.527 ± 0.104
3.876IleLys: 3.876 ± 0.082
6.528IleLeu: 6.528 ± 0.131
1.43IleMet: 1.43 ± 0.047
3.16IleAsn: 3.16 ± 0.076
2.824IlePro: 2.824 ± 0.06
2.619IleGln: 2.619 ± 0.066
2.902IleArg: 2.902 ± 0.067
4.863IleSer: 4.863 ± 0.091
3.822IleThr: 3.822 ± 0.082
4.644IleVal: 4.644 ± 0.102
0.672IleTrp: 0.672 ± 0.036
2.055IleTyr: 2.055 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
6.087LysAla: 6.087 ± 0.144
0.4LysCys: 0.4 ± 0.025
3.074LysAsp: 3.074 ± 0.174
4.196LysGlu: 4.196 ± 0.085
2.341LysPhe: 2.341 ± 0.056
4.074LysGly: 4.074 ± 0.089
1.207LysHis: 1.207 ± 0.046
4.191LysIle: 4.191 ± 0.081
3.833LysLys: 3.833 ± 0.111
6.251LysLeu: 6.251 ± 0.092
1.937LysMet: 1.937 ± 0.058
3.295LysAsn: 3.295 ± 0.076
2.518LysPro: 2.518 ± 0.063
3.103LysGln: 3.103 ± 0.07
2.89LysArg: 2.89 ± 0.076
3.472LysSer: 3.472 ± 0.078
3.463LysThr: 3.463 ± 0.074
4.47LysVal: 4.47 ± 0.099
0.712LysTrp: 0.712 ± 0.033
1.691LysTyr: 1.691 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
10.012LeuAla: 10.012 ± 0.114
0.953LeuCys: 0.953 ± 0.045
5.371LeuAsp: 5.371 ± 0.094
6.284LeuGlu: 6.284 ± 0.116
4.645LeuPhe: 4.645 ± 0.117
7.125LeuGly: 7.125 ± 0.135
1.865LeuHis: 1.865 ± 0.055
6.753LeuIle: 6.753 ± 0.119
5.995LeuLys: 5.995 ± 0.104
10.438LeuLeu: 10.438 ± 0.218
2.425LeuMet: 2.425 ± 0.072
5.242LeuAsn: 5.242 ± 0.101
4.732LeuPro: 4.732 ± 0.109
3.998LeuGln: 3.998 ± 0.093
4.244LeuArg: 4.244 ± 0.101
7.134LeuSer: 7.134 ± 0.14
5.704LeuThr: 5.704 ± 0.112
6.711LeuVal: 6.711 ± 0.123
1.013LeuTrp: 1.013 ± 0.043
2.637LeuTyr: 2.637 ± 0.067
0.0LeuXaa: 0.0 ± 0.0
Met
2.461MetAla: 2.461 ± 0.07
0.195MetCys: 0.195 ± 0.016
1.007MetAsp: 1.007 ± 0.037
1.28MetGlu: 1.28 ± 0.048
0.929MetPhe: 0.929 ± 0.032
1.64MetGly: 1.64 ± 0.053
0.429MetHis: 0.429 ± 0.025
1.578MetIle: 1.578 ± 0.055
1.644MetLys: 1.644 ± 0.046
2.62MetLeu: 2.62 ± 0.068
0.759MetMet: 0.759 ± 0.036
1.213MetAsn: 1.213 ± 0.041
1.127MetPro: 1.127 ± 0.044
1.246MetGln: 1.246 ± 0.047
1.0MetArg: 1.0 ± 0.04
1.572MetSer: 1.572 ± 0.05
1.436MetThr: 1.436 ± 0.051
1.59MetVal: 1.59 ± 0.046
0.217MetTrp: 0.217 ± 0.019
0.501MetTyr: 0.501 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
4.295AsnAla: 4.295 ± 0.139
0.421AsnCys: 0.421 ± 0.029
2.231AsnAsp: 2.231 ± 0.069
3.052AsnGlu: 3.052 ± 0.075
1.965AsnPhe: 1.965 ± 0.053
3.499AsnGly: 3.499 ± 0.12
0.964AsnHis: 0.964 ± 0.04
3.584AsnIle: 3.584 ± 0.08
2.707AsnLys: 2.707 ± 0.069
4.911AsnLeu: 4.911 ± 0.089
1.039AsnMet: 1.039 ± 0.038
2.365AsnAsn: 2.365 ± 0.104
2.542AsnPro: 2.542 ± 0.062
2.359AsnGln: 2.359 ± 0.069
2.009AsnArg: 2.009 ± 0.053
2.752AsnSer: 2.752 ± 0.076
2.247AsnThr: 2.247 ± 0.072
3.329AsnVal: 3.329 ± 0.097
0.646AsnTrp: 0.646 ± 0.033
1.632AsnTyr: 1.632 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.799ProAla: 2.799 ± 0.08
0.273ProCys: 0.273 ± 0.02
1.856ProAsp: 1.856 ± 0.055
3.235ProGlu: 3.235 ± 0.077
1.929ProPhe: 1.929 ± 0.052
1.396ProGly: 1.396 ± 0.053
0.871ProHis: 0.871 ± 0.037
2.713ProIle: 2.713 ± 0.064
2.31ProLys: 2.31 ± 0.068
3.912ProLeu: 3.912 ± 0.093
1.025ProMet: 1.025 ± 0.046
2.35ProAsn: 2.35 ± 0.062
1.07ProPro: 1.07 ± 0.042
1.79ProGln: 1.79 ± 0.057
1.288ProArg: 1.288 ± 0.047
2.079ProSer: 2.079 ± 0.054
2.466ProThr: 2.466 ± 0.07
2.679ProVal: 2.679 ± 0.08
0.331ProTrp: 0.331 ± 0.026
1.325ProTyr: 1.325 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
4.31GlnAla: 4.31 ± 0.089
0.307GlnCys: 0.307 ± 0.021
1.808GlnAsp: 1.808 ± 0.054
2.493GlnGlu: 2.493 ± 0.069
2.072GlnPhe: 2.072 ± 0.055
2.847GlnGly: 2.847 ± 0.07
0.968GlnHis: 0.968 ± 0.042
3.256GlnIle: 3.256 ± 0.078
3.01GlnLys: 3.01 ± 0.073
4.538GlnLeu: 4.538 ± 0.101
1.012GlnMet: 1.012 ± 0.037
2.281GlnAsn: 2.281 ± 0.066
1.799GlnPro: 1.799 ± 0.054
2.871GlnGln: 2.871 ± 0.089
2.03GlnArg: 2.03 ± 0.073
2.365GlnSer: 2.365 ± 0.073
2.184GlnThr: 2.184 ± 0.062
2.694GlnVal: 2.694 ± 0.061
0.562GlnTrp: 0.562 ± 0.029
1.411GlnTyr: 1.411 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
3.021ArgAla: 3.021 ± 0.078
0.349ArgCys: 0.349 ± 0.025
2.12ArgAsp: 2.12 ± 0.061
3.061ArgGlu: 3.061 ± 0.079
2.195ArgPhe: 2.195 ± 0.068
2.556ArgGly: 2.556 ± 0.065
0.91ArgHis: 0.91 ± 0.037
2.847ArgIle: 2.847 ± 0.068
2.613ArgLys: 2.613 ± 0.072
4.75ArgLeu: 4.75 ± 0.104
0.973ArgMet: 0.973 ± 0.041
2.022ArgAsn: 2.022 ± 0.057
1.539ArgPro: 1.539 ± 0.046
2.015ArgGln: 2.015 ± 0.057
1.983ArgArg: 1.983 ± 0.072
2.325ArgSer: 2.325 ± 0.069
1.95ArgThr: 1.95 ± 0.055
2.809ArgVal: 2.809 ± 0.079
0.52ArgTrp: 0.52 ± 0.03
1.685ArgTyr: 1.685 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
4.909SerAla: 4.909 ± 0.092
0.54SerCys: 0.54 ± 0.031
2.908SerAsp: 2.908 ± 0.067
3.855SerGlu: 3.855 ± 0.074
2.713SerPhe: 2.713 ± 0.075
4.65SerGly: 4.65 ± 0.085
1.325SerHis: 1.325 ± 0.051
3.915SerIle: 3.915 ± 0.087
3.175SerLys: 3.175 ± 0.07
6.261SerLeu: 6.261 ± 0.112
1.319SerMet: 1.319 ± 0.042
2.521SerAsn: 2.521 ± 0.075
2.302SerPro: 2.302 ± 0.069
2.764SerGln: 2.764 ± 0.069
2.472SerArg: 2.472 ± 0.068
3.577SerSer: 3.577 ± 0.104
2.97SerThr: 2.97 ± 0.075
3.938SerVal: 3.938 ± 0.082
0.696SerTrp: 0.696 ± 0.031
1.895SerTyr: 1.895 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
4.963ThrAla: 4.963 ± 0.108
0.42ThrCys: 0.42 ± 0.028
2.515ThrAsp: 2.515 ± 0.057
3.449ThrGlu: 3.449 ± 0.073
2.296ThrPhe: 2.296 ± 0.057
3.812ThrGly: 3.812 ± 0.085
1.087ThrHis: 1.087 ± 0.04
3.568ThrIle: 3.568 ± 0.077
2.851ThrLys: 2.851 ± 0.089
5.852ThrLeu: 5.852 ± 0.103
1.033ThrMet: 1.033 ± 0.038
2.19ThrAsn: 2.19 ± 0.094
2.485ThrPro: 2.485 ± 0.07
2.27ThrGln: 2.27 ± 0.057
1.863ThrArg: 1.863 ± 0.049
2.757ThrSer: 2.757 ± 0.065
2.919ThrThr: 2.919 ± 0.098
3.781ThrVal: 3.781 ± 0.125
0.526ThrTrp: 0.526 ± 0.027
1.435ThrTyr: 1.435 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
6.258ValAla: 6.258 ± 0.12
0.661ValCys: 0.661 ± 0.035
3.485ValAsp: 3.485 ± 0.078
5.046ValGlu: 5.046 ± 0.099
2.775ValPhe: 2.775 ± 0.066
4.822ValGly: 4.822 ± 0.108
1.09ValHis: 1.09 ± 0.043
4.956ValIle: 4.956 ± 0.095
4.47ValLys: 4.47 ± 0.096
6.668ValLeu: 6.668 ± 0.109
1.829ValMet: 1.829 ± 0.052
3.01ValAsn: 3.01 ± 0.102
2.61ValPro: 2.61 ± 0.064
2.475ValGln: 2.475 ± 0.076
2.797ValArg: 2.797 ± 0.065
4.221ValSer: 4.221 ± 0.091
3.605ValThr: 3.605 ± 0.109
5.19ValVal: 5.19 ± 0.113
0.682ValTrp: 0.682 ± 0.041
1.758ValTyr: 1.758 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
0.907TrpAla: 0.907 ± 0.042
0.126TrpCys: 0.126 ± 0.014
0.495TrpAsp: 0.495 ± 0.029
0.637TrpGlu: 0.637 ± 0.031
0.541TrpPhe: 0.541 ± 0.03
0.747TrpGly: 0.747 ± 0.036
0.274TrpHis: 0.274 ± 0.023
0.77TrpIle: 0.77 ± 0.04
0.676TrpLys: 0.676 ± 0.036
1.641TrpLeu: 1.641 ± 0.063
0.196TrpMet: 0.196 ± 0.016
0.538TrpAsn: 0.538 ± 0.028
0.136TrpPro: 0.136 ± 0.014
0.92TrpGln: 0.92 ± 0.042
0.525TrpArg: 0.525 ± 0.029
0.526TrpSer: 0.526 ± 0.03
0.445TrpThr: 0.445 ± 0.026
0.767TrpVal: 0.767 ± 0.033
0.156TrpTrp: 0.156 ± 0.014
0.348TrpTyr: 0.348 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.49TyrAla: 2.49 ± 0.063
0.363TyrCys: 0.363 ± 0.025
1.527TyrAsp: 1.527 ± 0.044
1.581TyrGlu: 1.581 ± 0.051
1.602TyrPhe: 1.602 ± 0.057
2.229TyrGly: 2.229 ± 0.061
0.76TyrHis: 0.76 ± 0.036
1.865TyrIle: 1.865 ± 0.062
1.49TyrLys: 1.49 ± 0.048
3.43TyrLeu: 3.43 ± 0.079
0.585TyrMet: 0.585 ± 0.032
1.343TyrAsn: 1.343 ± 0.05
1.43TyrPro: 1.43 ± 0.049
1.923TyrGln: 1.923 ± 0.064
1.691TyrArg: 1.691 ± 0.054
1.919TyrSer: 1.919 ± 0.054
1.536TyrThr: 1.536 ± 0.05
1.85TyrVal: 1.85 ± 0.049
0.412TyrTrp: 0.412 ± 0.023
1.016TyrTyr: 1.016 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2108 proteins (667106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski