Amino acid dipepetide frequency for Botrimarina hoheduenensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.821AlaAla: 16.821 ± 0.165	1.332AlaCys: 1.332 ± 0.035	7.194AlaAsp: 7.194 ± 0.091	8.297AlaGlu: 8.297 ± 0.087	3.516AlaPhe: 3.516 ± 0.059	10.187AlaGly: 10.187 ± 0.124	1.785AlaHis: 1.785 ± 0.041	5.777AlaIle: 5.777 ± 0.075	3.481AlaLys: 3.481 ± 0.088	11.106AlaLeu: 11.106 ± 0.128	2.533AlaMet: 2.533 ± 0.049	3.044AlaAsn: 3.044 ± 0.055	5.97AlaPro: 5.97 ± 0.088	3.998AlaGln: 3.998 ± 0.053	6.961AlaArg: 6.961 ± 0.089	7.102AlaSer: 7.102 ± 0.096	7.494AlaThr: 7.494 ± 0.094	8.374AlaVal: 8.374 ± 0.081	1.754AlaTrp: 1.754 ± 0.043	2.537AlaTyr: 2.537 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.873CysAla: 0.873 ± 0.03	0.28CysCys: 0.28 ± 0.033	0.679CysAsp: 0.679 ± 0.025	0.692CysGlu: 0.692 ± 0.031	0.343CysPhe: 0.343 ± 0.018	1.131CysGly: 1.131 ± 0.047	0.308CysHis: 0.308 ± 0.018	0.27CysIle: 0.27 ± 0.015	0.253CysLys: 0.253 ± 0.02	1.161CysLeu: 1.161 ± 0.03	0.13CysMet: 0.13 ± 0.009	0.233CysAsn: 0.233 ± 0.013	0.604CysPro: 0.604 ± 0.026	0.365CysGln: 0.365 ± 0.019	0.89CysArg: 0.89 ± 0.03	0.537CysSer: 0.537 ± 0.019	0.417CysThr: 0.417 ± 0.021	0.86CysVal: 0.86 ± 0.03	0.18CysTrp: 0.18 ± 0.013	0.276CysTyr: 0.276 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	6.68AspAla: 6.68 ± 0.081	0.545AspCys: 0.545 ± 0.023	3.583AspAsp: 3.583 ± 0.085	3.956AspGlu: 3.956 ± 0.071	2.331AspPhe: 2.331 ± 0.058	5.197AspGly: 5.197 ± 0.106	1.227AspHis: 1.227 ± 0.033	1.976AspIle: 1.976 ± 0.045	1.429AspLys: 1.429 ± 0.039	6.154AspLeu: 6.154 ± 0.094	0.777AspMet: 0.777 ± 0.026	1.663AspAsn: 1.663 ± 0.044	3.985AspPro: 3.985 ± 0.059	2.188AspGln: 2.188 ± 0.046	4.724AspArg: 4.724 ± 0.063	3.046AspSer: 3.046 ± 0.054	2.976AspThr: 2.976 ± 0.072	3.469AspVal: 3.469 ± 0.067	1.056AspTrp: 1.056 ± 0.028	1.853AspTyr: 1.853 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	7.691GluAla: 7.691 ± 0.103	0.48GluCys: 0.48 ± 0.025	2.699GluAsp: 2.699 ± 0.053	3.682GluGlu: 3.682 ± 0.079	1.885GluPhe: 1.885 ± 0.038	4.453GluGly: 4.453 ± 0.071	1.306GluHis: 1.306 ± 0.035	2.812GluIle: 2.812 ± 0.051	1.817GluLys: 1.817 ± 0.047	6.803GluLeu: 6.803 ± 0.077	1.2GluMet: 1.2 ± 0.031	1.494GluAsn: 1.494 ± 0.038	3.538GluPro: 3.538 ± 0.065	2.785GluGln: 2.785 ± 0.055	5.215GluArg: 5.215 ± 0.086	3.533GluSer: 3.533 ± 0.052	3.402GluThr: 3.402 ± 0.053	4.373GluVal: 4.373 ± 0.067	0.847GluTrp: 0.847 ± 0.025	1.263GluTyr: 1.263 ± 0.032	0.001GluXaa: 0.001 ± 0.001
Phe	3.763PheAla: 3.763 ± 0.066	0.437PheCys: 0.437 ± 0.022	2.675PheAsp: 2.675 ± 0.054	1.998PheGlu: 1.998 ± 0.034	1.281PhePhe: 1.281 ± 0.04	3.283PheGly: 3.283 ± 0.06	0.584PheHis: 0.584 ± 0.023	1.089PheIle: 1.089 ± 0.036	0.704PheLys: 0.704 ± 0.025	2.936PheLeu: 2.936 ± 0.055	0.515PheMet: 0.515 ± 0.021	1.145PheAsn: 1.145 ± 0.038	1.416PhePro: 1.416 ± 0.037	0.99PheGln: 0.99 ± 0.026	2.226PheArg: 2.226 ± 0.041	2.152PheSer: 2.152 ± 0.044	1.963PheThr: 1.963 ± 0.049	2.647PheVal: 2.647 ± 0.051	0.514PheTrp: 0.514 ± 0.02	0.911PheTyr: 0.911 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.599GlyAla: 8.599 ± 0.115	1.018GlyCys: 1.018 ± 0.042	5.188GlyAsp: 5.188 ± 0.118	5.022GlyGlu: 5.022 ± 0.065	3.141GlyPhe: 3.141 ± 0.054	7.756GlyGly: 7.756 ± 0.174	1.54GlyHis: 1.54 ± 0.042	3.304GlyIle: 3.304 ± 0.055	2.956GlyLys: 2.956 ± 0.066	8.069GlyLeu: 8.069 ± 0.089	1.886GlyMet: 1.886 ± 0.041	2.192GlyAsn: 2.192 ± 0.058	4.047GlyPro: 4.047 ± 0.063	3.036GlyGln: 3.036 ± 0.058	5.514GlyArg: 5.514 ± 0.081	5.321GlySer: 5.321 ± 0.103	4.335GlyThr: 4.335 ± 0.087	7.689GlyVal: 7.689 ± 0.09	1.505GlyTrp: 1.505 ± 0.039	2.287GlyTyr: 2.287 ± 0.046	0.004GlyXaa: 0.004 ± 0.002
His	2.29HisAla: 2.29 ± 0.045	0.245HisCys: 0.245 ± 0.017	1.172HisAsp: 1.172 ± 0.036	1.241HisGlu: 1.241 ± 0.035	0.702HisPhe: 0.702 ± 0.023	1.635HisGly: 1.635 ± 0.044	0.592HisHis: 0.592 ± 0.023	0.598HisIle: 0.598 ± 0.023	0.45HisLys: 0.45 ± 0.021	1.93HisLeu: 1.93 ± 0.046	0.246HisMet: 0.246 ± 0.014	0.546HisAsn: 0.546 ± 0.02	1.265HisPro: 1.265 ± 0.034	0.715HisGln: 0.715 ± 0.023	1.578HisArg: 1.578 ± 0.039	0.949HisSer: 0.949 ± 0.029	1.044HisThr: 1.044 ± 0.033	1.3HisVal: 1.3 ± 0.03	0.319HisTrp: 0.319 ± 0.018	0.56HisTyr: 0.56 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.095IleAla: 6.095 ± 0.074	0.356IleCys: 0.356 ± 0.018	4.015IleAsp: 4.015 ± 0.069	3.541IleGlu: 3.541 ± 0.064	1.051IlePhe: 1.051 ± 0.031	4.18IleGly: 4.18 ± 0.07	0.719IleHis: 0.719 ± 0.027	1.255IleIle: 1.255 ± 0.031	1.055IleLys: 1.055 ± 0.034	2.821IleLeu: 2.821 ± 0.053	0.483IleMet: 0.483 ± 0.022	1.401IleAsn: 1.401 ± 0.04	1.84IlePro: 1.84 ± 0.044	1.101IleGln: 1.101 ± 0.032	2.629IleArg: 2.629 ± 0.047	2.099IleSer: 2.099 ± 0.04	2.401IleThr: 2.401 ± 0.052	3.379IleVal: 3.379 ± 0.058	0.381IleTrp: 0.381 ± 0.02	0.881IleTyr: 0.881 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	2.66LysAla: 2.66 ± 0.061	0.215LysCys: 0.215 ± 0.016	1.145LysAsp: 1.145 ± 0.037	1.414LysGlu: 1.414 ± 0.043	0.71LysPhe: 0.71 ± 0.026	1.741LysGly: 1.741 ± 0.048	0.637LysHis: 0.637 ± 0.025	1.306LysIle: 1.306 ± 0.035	1.246LysLys: 1.246 ± 0.056	3.107LysLeu: 3.107 ± 0.064	0.516LysMet: 0.516 ± 0.017	0.785LysAsn: 0.785 ± 0.029	2.06LysPro: 2.06 ± 0.048	1.297LysGln: 1.297 ± 0.038	2.229LysArg: 2.229 ± 0.057	1.635LysSer: 1.635 ± 0.048	1.668LysThr: 1.668 ± 0.041	1.731LysVal: 1.731 ± 0.042	0.341LysTrp: 0.341 ± 0.015	0.662LysTyr: 0.662 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	14.051LeuAla: 14.051 ± 0.131	1.124LeuCys: 1.124 ± 0.033	6.0LeuAsp: 6.0 ± 0.069	5.8LeuGlu: 5.8 ± 0.083	3.149LeuPhe: 3.149 ± 0.06	8.428LeuGly: 8.428 ± 0.093	1.861LeuHis: 1.861 ± 0.04	4.176LeuIle: 4.176 ± 0.063	2.68LeuLys: 2.68 ± 0.057	11.185LeuLeu: 11.185 ± 0.137	1.955LeuMet: 1.955 ± 0.042	2.619LeuAsn: 2.619 ± 0.048	5.828LeuPro: 5.828 ± 0.087	3.134LeuGln: 3.134 ± 0.054	7.721LeuArg: 7.721 ± 0.093	6.45LeuSer: 6.45 ± 0.069	5.991LeuThr: 5.991 ± 0.062	8.064LeuVal: 8.064 ± 0.099	1.296LeuTrp: 1.296 ± 0.036	2.044LeuTyr: 2.044 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	2.144MetAla: 2.144 ± 0.049	0.144MetCys: 0.144 ± 0.009	0.741MetAsp: 0.741 ± 0.026	0.785MetGlu: 0.785 ± 0.027	0.496MetPhe: 0.496 ± 0.022	1.301MetGly: 1.301 ± 0.037	0.393MetHis: 0.393 ± 0.017	0.955MetIle: 0.955 ± 0.03	0.676MetLys: 0.676 ± 0.024	2.09MetLeu: 2.09 ± 0.043	0.413MetMet: 0.413 ± 0.019	0.632MetAsn: 0.632 ± 0.021	1.261MetPro: 1.261 ± 0.034	0.682MetGln: 0.682 ± 0.025	1.335MetArg: 1.335 ± 0.034	1.355MetSer: 1.355 ± 0.035	1.303MetThr: 1.303 ± 0.03	1.317MetVal: 1.317 ± 0.036	0.158MetTrp: 0.158 ± 0.011	0.329MetTyr: 0.329 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.165AsnAla: 3.165 ± 0.063	0.264AsnCys: 0.264 ± 0.013	1.768AsnAsp: 1.768 ± 0.049	1.582AsnGlu: 1.582 ± 0.038	0.98AsnPhe: 0.98 ± 0.034	2.585AsnGly: 2.585 ± 0.084	0.576AsnHis: 0.576 ± 0.021	0.787AsnIle: 0.787 ± 0.029	0.63AsnLys: 0.63 ± 0.023	2.862AsnLeu: 2.862 ± 0.053	0.36AsnMet: 0.36 ± 0.019	0.961AsnAsn: 0.961 ± 0.042	1.949AsnPro: 1.949 ± 0.047	1.038AsnGln: 1.038 ± 0.031	2.082AsnArg: 2.082 ± 0.042	1.428AsnSer: 1.428 ± 0.039	1.514AsnThr: 1.514 ± 0.046	1.797AsnVal: 1.797 ± 0.042	0.446AsnTrp: 0.446 ± 0.021	0.763AsnTyr: 0.763 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.16ProAla: 7.16 ± 0.093	0.455ProCys: 0.455 ± 0.022	3.195ProAsp: 3.195 ± 0.05	3.789ProGlu: 3.789 ± 0.054	1.744ProPhe: 1.744 ± 0.038	4.605ProGly: 4.605 ± 0.069	1.157ProHis: 1.157 ± 0.033	2.433ProIle: 2.433 ± 0.04	1.344ProLys: 1.344 ± 0.04	5.759ProLeu: 5.759 ± 0.092	1.036ProMet: 1.036 ± 0.032	1.688ProAsn: 1.688 ± 0.041	3.431ProPro: 3.431 ± 0.076	1.986ProGln: 1.986 ± 0.05	3.502ProArg: 3.502 ± 0.063	3.622ProSer: 3.622 ± 0.067	3.763ProThr: 3.763 ± 0.062	3.88ProVal: 3.88 ± 0.055	0.759ProTrp: 0.759 ± 0.025	1.13ProTyr: 1.13 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.333GlnAla: 4.333 ± 0.061	0.287GlnCys: 0.287 ± 0.015	1.38GlnAsp: 1.38 ± 0.038	1.765GlnGlu: 1.765 ± 0.04	1.042GlnPhe: 1.042 ± 0.026	2.505GlnGly: 2.505 ± 0.046	0.789GlnHis: 0.789 ± 0.023	1.789GlnIle: 1.789 ± 0.045	0.922GlnLys: 0.922 ± 0.026	4.157GlnLeu: 4.157 ± 0.064	0.796GlnMet: 0.796 ± 0.023	0.818GlnAsn: 0.818 ± 0.026	2.291GlnPro: 2.291 ± 0.046	1.962GlnGln: 1.962 ± 0.047	3.25GlnArg: 3.25 ± 0.057	2.087GlnSer: 2.087 ± 0.041	2.185GlnThr: 2.185 ± 0.049	2.44GlnVal: 2.44 ± 0.052	0.473GlnTrp: 0.473 ± 0.02	0.736GlnTyr: 0.736 ± 0.023	0.001GlnXaa: 0.001 ± 0.001
Arg	6.595ArgAla: 6.595 ± 0.09	0.853ArgCys: 0.853 ± 0.031	3.915ArgAsp: 3.915 ± 0.059	4.362ArgGlu: 4.362 ± 0.071	2.971ArgPhe: 2.971 ± 0.053	5.198ArgGly: 5.198 ± 0.064	1.541ArgHis: 1.541 ± 0.037	3.264ArgIle: 3.264 ± 0.052	1.908ArgLys: 1.908 ± 0.042	8.913ArgLeu: 8.913 ± 0.105	1.675ArgMet: 1.675 ± 0.033	1.732ArgAsn: 1.732 ± 0.038	3.51ArgPro: 3.51 ± 0.056	2.841ArgGln: 2.841 ± 0.061	6.464ArgArg: 6.464 ± 0.097	4.234ArgSer: 4.234 ± 0.065	3.532ArgThr: 3.532 ± 0.06	5.67ArgVal: 5.67 ± 0.067	1.358ArgTrp: 1.358 ± 0.036	2.033ArgTyr: 2.033 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.733SerAla: 6.733 ± 0.082	0.582SerCys: 0.582 ± 0.037	3.45SerAsp: 3.45 ± 0.06	3.344SerGlu: 3.344 ± 0.056	2.029SerPhe: 2.029 ± 0.045	5.626SerGly: 5.626 ± 0.082	1.105SerHis: 1.105 ± 0.032	2.502SerIle: 2.502 ± 0.044	1.475SerLys: 1.475 ± 0.039	6.216SerLeu: 6.216 ± 0.078	1.077SerMet: 1.077 ± 0.03	1.592SerAsn: 1.592 ± 0.043	3.711SerPro: 3.711 ± 0.059	2.088SerGln: 2.088 ± 0.041	3.995SerArg: 3.995 ± 0.059	3.696SerSer: 3.696 ± 0.063	3.42SerThr: 3.42 ± 0.064	4.377SerVal: 4.377 ± 0.068	0.829SerTrp: 0.829 ± 0.025	1.395SerTyr: 1.395 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.507ThrAla: 6.507 ± 0.08	0.506ThrCys: 0.506 ± 0.024	3.323ThrAsp: 3.323 ± 0.062	2.924ThrGlu: 2.924 ± 0.051	1.851ThrPhe: 1.851 ± 0.048	5.238ThrGly: 5.238 ± 0.093	1.147ThrHis: 1.147 ± 0.031	2.85ThrIle: 2.85 ± 0.063	1.398ThrLys: 1.398 ± 0.038	6.68ThrLeu: 6.68 ± 0.093	0.882ThrMet: 0.882 ± 0.028	1.641ThrAsn: 1.641 ± 0.042	4.463ThrPro: 4.463 ± 0.064	1.914ThrGln: 1.914 ± 0.039	3.256ThrArg: 3.256 ± 0.056	3.25ThrSer: 3.25 ± 0.056	3.604ThrThr: 3.604 ± 0.065	4.268ThrVal: 4.268 ± 0.083	0.696ThrTrp: 0.696 ± 0.025	1.203ThrTyr: 1.203 ± 0.032	0.002ThrXaa: 0.002 ± 0.001
Val	9.271ValAla: 9.271 ± 0.08	0.839ValCys: 0.839 ± 0.033	4.282ValAsp: 4.282 ± 0.063	4.843ValGlu: 4.843 ± 0.081	2.525ValPhe: 2.525 ± 0.05	6.223ValGly: 6.223 ± 0.076	1.264ValHis: 1.264 ± 0.033	3.261ValIle: 3.261 ± 0.063	1.755ValLys: 1.755 ± 0.04	7.409ValLeu: 7.409 ± 0.092	1.426ValMet: 1.426 ± 0.04	2.089ValAsn: 2.089 ± 0.055	3.438ValPro: 3.438 ± 0.058	2.24ValGln: 2.24 ± 0.037	5.421ValArg: 5.421 ± 0.064	4.513ValSer: 4.513 ± 0.065	4.548ValThr: 4.548 ± 0.069	6.378ValVal: 6.378 ± 0.082	1.157ValTrp: 1.157 ± 0.037	1.677ValTyr: 1.677 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	1.234TrpAla: 1.234 ± 0.034	0.216TrpCys: 0.216 ± 0.012	0.772TrpAsp: 0.772 ± 0.027	0.678TrpGlu: 0.678 ± 0.029	0.545TrpPhe: 0.545 ± 0.023	1.037TrpGly: 1.037 ± 0.035	0.342TrpHis: 0.342 ± 0.017	0.711TrpIle: 0.711 ± 0.022	0.409TrpLys: 0.409 ± 0.018	1.78TrpLeu: 1.78 ± 0.051	0.31TrpMet: 0.31 ± 0.015	0.5TrpAsn: 0.5 ± 0.023	0.757TrpPro: 0.757 ± 0.026	0.678TrpGln: 0.678 ± 0.026	1.525TrpArg: 1.525 ± 0.038	0.972TrpSer: 0.972 ± 0.034	0.773TrpThr: 0.773 ± 0.028	0.904TrpVal: 0.904 ± 0.028	0.305TrpTrp: 0.305 ± 0.015	0.308TrpTyr: 0.308 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.598TyrAla: 2.598 ± 0.042	0.304TyrCys: 0.304 ± 0.018	1.643TyrAsp: 1.643 ± 0.044	1.564TyrGlu: 1.564 ± 0.041	0.936TyrPhe: 0.936 ± 0.029	2.098TyrGly: 2.098 ± 0.046	0.505TyrHis: 0.505 ± 0.024	0.568TyrIle: 0.568 ± 0.021	0.515TyrLys: 0.515 ± 0.022	2.415TyrLeu: 2.415 ± 0.045	0.293TyrMet: 0.293 ± 0.016	0.762TyrAsn: 0.762 ± 0.024	1.07TyrPro: 1.07 ± 0.031	0.969TyrGln: 0.969 ± 0.028	2.052TyrArg: 2.052 ± 0.045	1.261TyrSer: 1.261 ± 0.037	1.269TyrThr: 1.269 ± 0.037	1.644TyrVal: 1.644 ± 0.034	0.382TyrTrp: 0.382 ± 0.017	0.723TyrTyr: 0.723 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.007XaaXaa: 0.007 ± 0.006

Statistics based on 3424 proteins (1258017 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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