Amino acid dipepetide frequency for Alkalihalobacillus macyae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.471AlaAla: 5.471 ± 0.083
0.56AlaCys: 0.56 ± 0.025
3.295AlaAsp: 3.295 ± 0.064
4.463AlaGlu: 4.463 ± 0.067
3.286AlaPhe: 3.286 ± 0.061
5.36AlaGly: 5.36 ± 0.089
1.28AlaHis: 1.28 ± 0.039
5.822AlaIle: 5.822 ± 0.069
4.153AlaLys: 4.153 ± 0.065
7.098AlaLeu: 7.098 ± 0.082
2.112AlaMet: 2.112 ± 0.045
2.764AlaAsn: 2.764 ± 0.059
2.122AlaPro: 2.122 ± 0.043
1.945AlaGln: 1.945 ± 0.039
2.769AlaArg: 2.769 ± 0.056
4.569AlaSer: 4.569 ± 0.061
3.7AlaThr: 3.7 ± 0.067
5.415AlaVal: 5.415 ± 0.071
0.602AlaTrp: 0.602 ± 0.027
2.316AlaTyr: 2.316 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.428CysAla: 0.428 ± 0.019
0.093CysCys: 0.093 ± 0.01
0.404CysAsp: 0.404 ± 0.018
0.461CysGlu: 0.461 ± 0.02
0.305CysPhe: 0.305 ± 0.015
0.603CysGly: 0.603 ± 0.023
0.198CysHis: 0.198 ± 0.014
0.452CysIle: 0.452 ± 0.021
0.33CysLys: 0.33 ± 0.018
0.599CysLeu: 0.599 ± 0.024
0.176CysMet: 0.176 ± 0.013
0.24CysAsn: 0.24 ± 0.014
0.345CysPro: 0.345 ± 0.019
0.203CysGln: 0.203 ± 0.014
0.289CysArg: 0.289 ± 0.017
0.484CysSer: 0.484 ± 0.019
0.355CysThr: 0.355 ± 0.017
0.384CysVal: 0.384 ± 0.022
0.061CysTrp: 0.061 ± 0.006
0.239CysTyr: 0.239 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.359AspAla: 3.359 ± 0.062
0.375AspCys: 0.375 ± 0.02
2.647AspAsp: 2.647 ± 0.048
4.71AspGlu: 4.71 ± 0.069
2.301AspPhe: 2.301 ± 0.051
3.639AspGly: 3.639 ± 0.061
1.353AspHis: 1.353 ± 0.039
3.705AspIle: 3.705 ± 0.062
2.921AspLys: 2.921 ± 0.053
5.361AspLeu: 5.361 ± 0.073
1.334AspMet: 1.334 ± 0.034
1.803AspAsn: 1.803 ± 0.041
2.123AspPro: 2.123 ± 0.048
2.408AspGln: 2.408 ± 0.043
2.594AspArg: 2.594 ± 0.047
2.74AspSer: 2.74 ± 0.046
2.47AspThr: 2.47 ± 0.047
4.054AspVal: 4.054 ± 0.064
0.669AspTrp: 0.669 ± 0.026
2.243AspTyr: 2.243 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
5.696GluAla: 5.696 ± 0.074
0.396GluCys: 0.396 ± 0.02
4.173GluAsp: 4.173 ± 0.065
7.876GluGlu: 7.876 ± 0.119
2.567GluPhe: 2.567 ± 0.052
4.885GluGly: 4.885 ± 0.064
1.475GluHis: 1.475 ± 0.038
5.046GluIle: 5.046 ± 0.075
5.964GluLys: 5.964 ± 0.079
7.446GluLeu: 7.446 ± 0.089
2.369GluMet: 2.369 ± 0.048
3.571GluAsn: 3.571 ± 0.064
2.11GluPro: 2.11 ± 0.046
3.196GluGln: 3.196 ± 0.056
3.993GluArg: 3.993 ± 0.071
4.036GluSer: 4.036 ± 0.063
4.063GluThr: 4.063 ± 0.062
5.46GluVal: 5.46 ± 0.068
0.944GluTrp: 0.944 ± 0.029
2.214GluTyr: 2.214 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
2.849PheAla: 2.849 ± 0.051
0.316PheCys: 0.316 ± 0.015
2.359PheAsp: 2.359 ± 0.051
2.921PheGlu: 2.921 ± 0.056
2.268PhePhe: 2.268 ± 0.048
3.342PheGly: 3.342 ± 0.061
1.029PheHis: 1.029 ± 0.031
3.541PheIle: 3.541 ± 0.066
2.164PheLys: 2.164 ± 0.046
4.486PheLeu: 4.486 ± 0.067
1.124PheMet: 1.124 ± 0.033
1.83PheAsn: 1.83 ± 0.036
1.701PhePro: 1.701 ± 0.038
1.647PheGln: 1.647 ± 0.038
1.565PheArg: 1.565 ± 0.035
3.183PheSer: 3.183 ± 0.053
2.538PheThr: 2.538 ± 0.048
3.175PheVal: 3.175 ± 0.056
0.469PheTrp: 0.469 ± 0.022
1.713PheTyr: 1.713 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
5.059GlyAla: 5.059 ± 0.084
0.57GlyCys: 0.57 ± 0.023
3.467GlyAsp: 3.467 ± 0.068
4.881GlyGlu: 4.881 ± 0.073
3.457GlyPhe: 3.457 ± 0.052
5.147GlyGly: 5.147 ± 0.081
1.358GlyHis: 1.358 ± 0.034
5.929GlyIle: 5.929 ± 0.08
4.735GlyLys: 4.735 ± 0.064
6.827GlyLeu: 6.827 ± 0.091
2.249GlyMet: 2.249 ± 0.043
2.786GlyAsn: 2.786 ± 0.053
1.81GlyPro: 1.81 ± 0.045
2.149GlyGln: 2.149 ± 0.043
2.708GlyArg: 2.708 ± 0.046
4.443GlySer: 4.443 ± 0.071
4.212GlyThr: 4.212 ± 0.066
5.418GlyVal: 5.418 ± 0.075
0.801GlyTrp: 0.801 ± 0.031
2.85GlyTyr: 2.85 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.354HisAla: 1.354 ± 0.037
0.177HisCys: 0.177 ± 0.011
1.088HisAsp: 1.088 ± 0.033
1.477HisGlu: 1.477 ± 0.037
1.08HisPhe: 1.08 ± 0.028
1.389HisGly: 1.389 ± 0.036
0.769HisHis: 0.769 ± 0.026
1.41HisIle: 1.41 ± 0.035
1.021HisLys: 1.021 ± 0.032
2.129HisLeu: 2.129 ± 0.049
0.512HisMet: 0.512 ± 0.026
0.815HisAsn: 0.815 ± 0.025
1.144HisPro: 1.144 ± 0.033
0.847HisGln: 0.847 ± 0.027
0.931HisArg: 0.931 ± 0.03
1.375HisSer: 1.375 ± 0.038
1.092HisThr: 1.092 ± 0.03
1.481HisVal: 1.481 ± 0.036
0.202HisTrp: 0.202 ± 0.013
0.976HisTyr: 0.976 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.719IleAla: 5.719 ± 0.086
0.52IleCys: 0.52 ± 0.024
4.102IleAsp: 4.102 ± 0.059
5.671IleGlu: 5.671 ± 0.067
2.807IlePhe: 2.807 ± 0.051
6.161IleGly: 6.161 ± 0.091
1.697IleHis: 1.697 ± 0.038
5.119IleIle: 5.119 ± 0.099
4.001IleLys: 4.001 ± 0.061
6.809IleLeu: 6.809 ± 0.086
1.784IleMet: 1.784 ± 0.046
2.88IleAsn: 2.88 ± 0.048
3.216IlePro: 3.216 ± 0.054
2.831IleGln: 2.831 ± 0.054
2.996IleArg: 2.996 ± 0.055
4.984IleSer: 4.984 ± 0.069
4.201IleThr: 4.201 ± 0.074
5.445IleVal: 5.445 ± 0.076
0.624IleTrp: 0.624 ± 0.025
2.25IleTyr: 2.25 ± 0.045
0.0IleXaa: 0.0 ± 0.0
Lys
4.457LysAla: 4.457 ± 0.068
0.279LysCys: 0.279 ± 0.017
3.57LysAsp: 3.57 ± 0.066
6.611LysGlu: 6.611 ± 0.087
1.708LysPhe: 1.708 ± 0.042
4.351LysGly: 4.351 ± 0.065
1.312LysHis: 1.312 ± 0.035
3.536LysIle: 3.536 ± 0.059
5.078LysLys: 5.078 ± 0.091
5.451LysLeu: 5.451 ± 0.071
1.962LysMet: 1.962 ± 0.04
2.753LysAsn: 2.753 ± 0.055
2.235LysPro: 2.235 ± 0.046
2.914LysGln: 2.914 ± 0.06
3.389LysArg: 3.389 ± 0.059
3.542LysSer: 3.542 ± 0.062
3.199LysThr: 3.199 ± 0.055
4.413LysVal: 4.413 ± 0.068
0.842LysTrp: 0.842 ± 0.032
1.752LysTyr: 1.752 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
7.04LeuAla: 7.04 ± 0.082
0.591LeuCys: 0.591 ± 0.025
4.962LeuAsp: 4.962 ± 0.072
6.6LeuGlu: 6.6 ± 0.069
4.947LeuPhe: 4.947 ± 0.084
6.414LeuGly: 6.414 ± 0.087
2.028LeuHis: 2.028 ± 0.042
7.193LeuIle: 7.193 ± 0.094
5.91LeuLys: 5.91 ± 0.084
10.357LeuLeu: 10.357 ± 0.129
2.553LeuMet: 2.553 ± 0.051
4.287LeuAsn: 4.287 ± 0.061
4.041LeuPro: 4.041 ± 0.067
3.385LeuGln: 3.385 ± 0.059
3.697LeuArg: 3.697 ± 0.069
7.516LeuSer: 7.516 ± 0.091
5.912LeuThr: 5.912 ± 0.079
6.579LeuVal: 6.579 ± 0.084
0.823LeuTrp: 0.823 ± 0.03
3.159LeuTyr: 3.159 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
1.994MetAla: 1.994 ± 0.044
0.144MetCys: 0.144 ± 0.011
1.446MetAsp: 1.446 ± 0.035
1.944MetGlu: 1.944 ± 0.043
0.993MetPhe: 0.993 ± 0.028
1.889MetGly: 1.889 ± 0.039
0.425MetHis: 0.425 ± 0.018
2.315MetIle: 2.315 ± 0.05
2.614MetLys: 2.614 ± 0.044
2.574MetLeu: 2.574 ± 0.05
0.953MetMet: 0.953 ± 0.034
1.713MetAsn: 1.713 ± 0.035
1.019MetPro: 1.019 ± 0.033
0.867MetGln: 0.867 ± 0.029
1.133MetArg: 1.133 ± 0.029
1.682MetSer: 1.682 ± 0.045
1.706MetThr: 1.706 ± 0.035
1.821MetVal: 1.821 ± 0.041
0.234MetTrp: 0.234 ± 0.013
0.726MetTyr: 0.726 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.798AsnAla: 2.798 ± 0.059
0.283AsnCys: 0.283 ± 0.018
2.377AsnAsp: 2.377 ± 0.047
3.905AsnGlu: 3.905 ± 0.058
1.459AsnPhe: 1.459 ± 0.037
3.405AsnGly: 3.405 ± 0.066
1.068AsnHis: 1.068 ± 0.029
2.684AsnIle: 2.684 ± 0.054
2.59AsnLys: 2.59 ± 0.054
3.789AsnLeu: 3.789 ± 0.057
1.079AsnMet: 1.079 ± 0.029
1.81AsnAsn: 1.81 ± 0.047
2.113AsnPro: 2.113 ± 0.05
1.882AsnGln: 1.882 ± 0.044
2.163AsnArg: 2.163 ± 0.045
2.352AsnSer: 2.352 ± 0.049
2.132AsnThr: 2.132 ± 0.04
3.216AsnVal: 3.216 ± 0.058
0.568AsnTrp: 0.568 ± 0.024
1.49AsnTyr: 1.49 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
2.278ProAla: 2.278 ± 0.042
0.188ProCys: 0.188 ± 0.013
2.279ProAsp: 2.279 ± 0.046
3.234ProGlu: 3.234 ± 0.057
1.987ProPhe: 1.987 ± 0.045
2.576ProGly: 2.576 ± 0.063
0.781ProHis: 0.781 ± 0.03
2.83ProIle: 2.83 ± 0.056
1.908ProLys: 1.908 ± 0.042
3.572ProLeu: 3.572 ± 0.066
0.895ProMet: 0.895 ± 0.027
1.656ProAsn: 1.656 ± 0.038
1.039ProPro: 1.039 ± 0.03
0.969ProGln: 0.969 ± 0.029
1.076ProArg: 1.076 ± 0.033
2.447ProSer: 2.447 ± 0.045
2.031ProThr: 2.031 ± 0.044
2.906ProVal: 2.906 ± 0.051
0.384ProTrp: 0.384 ± 0.018
1.451ProTyr: 1.451 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.461GlnAla: 2.461 ± 0.054
0.191GlnCys: 0.191 ± 0.014
1.788GlnAsp: 1.788 ± 0.042
2.733GlnGlu: 2.733 ± 0.055
1.571GlnPhe: 1.571 ± 0.034
2.069GlnGly: 2.069 ± 0.045
0.768GlnHis: 0.768 ± 0.025
2.272GlnIle: 2.272 ± 0.042
2.585GlnLys: 2.585 ± 0.053
3.908GlnLeu: 3.908 ± 0.067
1.122GlnMet: 1.122 ± 0.033
1.728GlnAsn: 1.728 ± 0.041
1.178GlnPro: 1.178 ± 0.037
1.572GlnGln: 1.572 ± 0.045
1.449GlnArg: 1.449 ± 0.04
2.388GlnSer: 2.388 ± 0.049
1.955GlnThr: 1.955 ± 0.042
2.398GlnVal: 2.398 ± 0.043
0.367GlnTrp: 0.367 ± 0.019
1.258GlnTyr: 1.258 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.499ArgAla: 2.499 ± 0.049
0.274ArgCys: 0.274 ± 0.017
2.25ArgAsp: 2.25 ± 0.044
3.375ArgGlu: 3.375 ± 0.065
1.895ArgPhe: 1.895 ± 0.04
2.429ArgGly: 2.429 ± 0.049
0.775ArgHis: 0.775 ± 0.029
3.179ArgIle: 3.179 ± 0.055
3.18ArgLys: 3.18 ± 0.052
4.053ArgLeu: 4.053 ± 0.062
1.42ArgMet: 1.42 ± 0.038
1.998ArgAsn: 1.998 ± 0.042
1.308ArgPro: 1.308 ± 0.034
1.353ArgGln: 1.353 ± 0.039
1.934ArgArg: 1.934 ± 0.045
2.696ArgSer: 2.696 ± 0.051
2.276ArgThr: 2.276 ± 0.049
2.824ArgVal: 2.824 ± 0.053
0.418ArgTrp: 0.418 ± 0.02
1.678ArgTyr: 1.678 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
3.866SerAla: 3.866 ± 0.066
0.429SerCys: 0.429 ± 0.021
3.308SerAsp: 3.308 ± 0.055
4.579SerGlu: 4.579 ± 0.06
3.424SerPhe: 3.424 ± 0.064
4.82SerGly: 4.82 ± 0.085
1.285SerHis: 1.285 ± 0.036
5.326SerIle: 5.326 ± 0.073
3.815SerLys: 3.815 ± 0.059
6.538SerLeu: 6.538 ± 0.088
1.856SerMet: 1.856 ± 0.042
2.831SerAsn: 2.831 ± 0.056
2.24SerPro: 2.24 ± 0.044
1.922SerGln: 1.922 ± 0.038
2.453SerArg: 2.453 ± 0.051
4.686SerSer: 4.686 ± 0.072
3.473SerThr: 3.473 ± 0.05
4.517SerVal: 4.517 ± 0.068
0.68SerTrp: 0.68 ± 0.029
2.431SerTyr: 2.431 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.018ThrAla: 4.018 ± 0.077
0.37ThrCys: 0.37 ± 0.018
2.848ThrAsp: 2.848 ± 0.058
3.593ThrGlu: 3.593 ± 0.062
2.701ThrPhe: 2.701 ± 0.041
4.272ThrGly: 4.272 ± 0.063
1.045ThrHis: 1.045 ± 0.026
4.694ThrIle: 4.694 ± 0.069
3.248ThrLys: 3.248 ± 0.057
5.317ThrLeu: 5.317 ± 0.062
1.401ThrMet: 1.401 ± 0.038
2.543ThrAsn: 2.543 ± 0.06
2.236ThrPro: 2.236 ± 0.051
1.416ThrGln: 1.416 ± 0.035
1.901ThrArg: 1.901 ± 0.043
3.54ThrSer: 3.54 ± 0.061
3.142ThrThr: 3.142 ± 0.074
4.287ThrVal: 4.287 ± 0.083
0.522ThrTrp: 0.522 ± 0.023
2.108ThrTyr: 2.108 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
5.014ValAla: 5.014 ± 0.071
0.534ValCys: 0.534 ± 0.025
3.89ValAsp: 3.89 ± 0.057
5.129ValGlu: 5.129 ± 0.064
3.197ValPhe: 3.197 ± 0.054
4.908ValGly: 4.908 ± 0.077
1.488ValHis: 1.488 ± 0.035
5.713ValIle: 5.713 ± 0.087
4.487ValLys: 4.487 ± 0.061
7.013ValLeu: 7.013 ± 0.076
2.047ValMet: 2.047 ± 0.042
3.191ValAsn: 3.191 ± 0.06
2.766ValPro: 2.766 ± 0.043
2.407ValGln: 2.407 ± 0.048
2.717ValArg: 2.717 ± 0.045
4.917ValSer: 4.917 ± 0.067
4.401ValThr: 4.401 ± 0.074
5.3ValVal: 5.3 ± 0.081
0.618ValTrp: 0.618 ± 0.022
2.308ValTyr: 2.308 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.614TrpAla: 0.614 ± 0.025
0.074TrpCys: 0.074 ± 0.008
0.547TrpAsp: 0.547 ± 0.023
0.687TrpGlu: 0.687 ± 0.025
0.524TrpPhe: 0.524 ± 0.024
0.707TrpGly: 0.707 ± 0.027
0.203TrpHis: 0.203 ± 0.013
0.856TrpIle: 0.856 ± 0.03
0.729TrpLys: 0.729 ± 0.027
1.091TrpLeu: 1.091 ± 0.035
0.363TrpMet: 0.363 ± 0.019
0.561TrpAsn: 0.561 ± 0.024
0.259TrpPro: 0.259 ± 0.014
0.385TrpGln: 0.385 ± 0.02
0.392TrpArg: 0.392 ± 0.018
0.686TrpSer: 0.686 ± 0.024
0.511TrpThr: 0.511 ± 0.024
0.689TrpVal: 0.689 ± 0.026
0.147TrpTrp: 0.147 ± 0.012
0.361TrpTyr: 0.361 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.128TyrAla: 2.128 ± 0.038
0.29TyrCys: 0.29 ± 0.017
2.01TyrAsp: 2.01 ± 0.047
2.63TyrGlu: 2.63 ± 0.05
1.742TyrPhe: 1.742 ± 0.038
2.488TyrGly: 2.488 ± 0.051
0.928TyrHis: 0.928 ± 0.029
2.196TyrIle: 2.196 ± 0.052
1.92TyrLys: 1.92 ± 0.038
3.573TyrLeu: 3.573 ± 0.065
0.882TyrMet: 0.882 ± 0.026
1.404TyrAsn: 1.404 ± 0.037
1.483TyrPro: 1.483 ± 0.039
1.462TyrGln: 1.462 ± 0.034
1.665TyrArg: 1.665 ± 0.039
2.212TyrSer: 2.212 ± 0.043
1.833TyrThr: 1.833 ± 0.046
2.263TyrVal: 2.263 ± 0.047
0.412TyrTrp: 0.412 ± 0.019
1.406TyrTyr: 1.406 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3973 proteins (1154936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski