Brettanomyces bruxellensis AWRI1499

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Pichiaceae; Brettanomyces; Brettanomyces bruxellensis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2JTY5|I2JTY5_DEKBR Basic leucine zipper transcription factor of the atf creb activates transcription of OS=Brettanomyces bruxellensis AWRI1499 OX=1124627 GN=AWRI1499_3678 PE=4 SV=2
MM1 pKa = 7.8RR2 pKa = 11.84FGALSLAALAGVALADD18 pKa = 3.7LDD20 pKa = 3.89ARR22 pKa = 11.84QVEE25 pKa = 4.48SLEE28 pKa = 3.99VLFLDD33 pKa = 5.11IEE35 pKa = 4.75DD36 pKa = 4.95NINGYY41 pKa = 8.12IQYY44 pKa = 11.35LMLNPGVSFPDD55 pKa = 3.53GLIALFTQIATYY67 pKa = 10.29TDD69 pKa = 4.14DD70 pKa = 3.64SFSTLFVTMSEE81 pKa = 4.22SEE83 pKa = 3.97YY84 pKa = 11.44DD85 pKa = 3.78EE86 pKa = 4.44IQSLEE91 pKa = 4.23TALPWYY97 pKa = 7.39STRR100 pKa = 11.84ILPKK104 pKa = 8.68ITIEE108 pKa = 4.31VTSSTSSTSTPVTSSSSTTVPTTVKK133 pKa = 9.67STTSTSATTSSTSTTTTSTTTTPSSTSSTTTPSTTSSTSSNPSTTTTPSTTSSTTSSTSSSEE195 pKa = 4.07STTSSVASSTSSSEE209 pKa = 3.98STTEE213 pKa = 3.66ATTSSSSSSSEE224 pKa = 3.59ATTEE228 pKa = 3.94ATTSLSTSSSEE239 pKa = 4.0SSSALEE245 pKa = 3.93TSAVSSSATSSDD257 pKa = 3.06AVTSADD263 pKa = 3.38SSLSSTLYY271 pKa = 8.75FTSAANSSAAAVTSVASSSTDD292 pKa = 3.29LAATXPVNASSSYY305 pKa = 10.61SDD307 pKa = 2.98SXLFSNSTSISATSXAXTAAASGSADD333 pKa = 3.3VVTTIVTITSCSDD346 pKa = 3.3HH347 pKa = 6.83VCTKK351 pKa = 9.8TAVTTGVKK359 pKa = 10.42SFTTTEE365 pKa = 4.08NGTKK369 pKa = 10.03TILTTYY375 pKa = 9.89CPLSSVSKK383 pKa = 9.51TSAIQATYY391 pKa = 10.87SNTPATEE398 pKa = 3.76TTAAVPXXSTSXSSEE413 pKa = 3.87STASEE418 pKa = 3.89AAXVSTYY425 pKa = 10.66EE426 pKa = 3.77GXAVKK431 pKa = 10.48FGYY434 pKa = 10.1SSNLMAAAAALLLLL448 pKa = 4.61

Molecular weight: 44.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2JWI0|I2JWI0_DEKBR Cbc2p OS=Brettanomyces bruxellensis AWRI1499 OX=1124627 GN=AWRI1499_2782 PE=3 SV=2
MM1 pKa = 6.82XCSXLXIQLAXLPLTRR17 pKa = 11.84SYY19 pKa = 12.0ANGPLKK25 pKa = 10.31ILSALPQRR33 pKa = 11.84PLKK36 pKa = 10.17KK37 pKa = 9.89IRR39 pKa = 11.84IGKK42 pKa = 8.89ARR44 pKa = 11.84PAIYY48 pKa = 10.55YY49 pKa = 9.71KK50 pKa = 10.75FNTFIEE56 pKa = 4.49LSDD59 pKa = 3.53GSVIRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84SQYY69 pKa = 9.53PRR71 pKa = 11.84EE72 pKa = 4.27EE73 pKa = 3.89LRR75 pKa = 11.84MITDD79 pKa = 3.28QRR81 pKa = 11.84NNPLWNPSKK90 pKa = 10.8AGCC93 pKa = 4.18

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4857

0

4857

1731709

62

2760

356.5

39.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.182 ± 0.037

1.279 ± 0.013

6.173 ± 0.027

6.622 ± 0.041

4.155 ± 0.027

5.761 ± 0.032

1.995 ± 0.015

5.889 ± 0.026

7.233 ± 0.039

8.911 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.015

4.934 ± 0.025

4.154 ± 0.025

3.767 ± 0.029

4.88 ± 0.029

8.715 ± 0.052

5.3 ± 0.029

5.822 ± 0.026

0.999 ± 0.012

3.322 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski