Amino acid dipepetide frequency for Salmonella phage FSLSP004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.933AlaAla: 10.933 ± 2.137
0.765AlaCys: 0.765 ± 0.294
6.341AlaAsp: 6.341 ± 1.01
5.794AlaGlu: 5.794 ± 0.981
2.952AlaPhe: 2.952 ± 0.52
8.199AlaGly: 8.199 ± 1.26
1.64AlaHis: 1.64 ± 0.37
4.701AlaIle: 4.701 ± 0.722
4.592AlaLys: 4.592 ± 0.735
10.714AlaLeu: 10.714 ± 1.012
1.859AlaMet: 1.859 ± 0.38
2.187AlaAsn: 2.187 ± 0.594
5.685AlaPro: 5.685 ± 0.908
4.92AlaGln: 4.92 ± 0.759
5.576AlaArg: 5.576 ± 1.033
7.653AlaSer: 7.653 ± 1.013
6.669AlaThr: 6.669 ± 1.082
6.997AlaVal: 6.997 ± 0.806
1.968AlaTrp: 1.968 ± 0.453
3.061AlaTyr: 3.061 ± 0.526
0.0AlaXaa: 0.0 ± 0.0
Cys
0.984CysAla: 0.984 ± 0.439
0.0CysCys: 0.0 ± 0.0
0.765CysAsp: 0.765 ± 0.292
0.328CysGlu: 0.328 ± 0.195
0.109CysPhe: 0.109 ± 0.106
0.656CysGly: 0.656 ± 0.248
0.219CysHis: 0.219 ± 0.155
0.547CysIle: 0.547 ± 0.224
0.328CysLys: 0.328 ± 0.176
0.547CysLeu: 0.547 ± 0.229
0.219CysMet: 0.219 ± 0.162
0.328CysAsn: 0.328 ± 0.173
0.437CysPro: 0.437 ± 0.312
0.656CysGln: 0.656 ± 0.295
0.765CysArg: 0.765 ± 0.271
0.437CysSer: 0.437 ± 0.214
0.875CysThr: 0.875 ± 0.298
0.547CysVal: 0.547 ± 0.229
0.109CysTrp: 0.109 ± 0.107
0.328CysTyr: 0.328 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
6.013AspAla: 6.013 ± 0.778
0.437AspCys: 0.437 ± 0.225
4.373AspAsp: 4.373 ± 0.809
4.154AspGlu: 4.154 ± 0.755
2.842AspPhe: 2.842 ± 0.655
5.904AspGly: 5.904 ± 0.534
0.547AspHis: 0.547 ± 0.225
4.482AspIle: 4.482 ± 0.761
3.498AspLys: 3.498 ± 0.685
3.936AspLeu: 3.936 ± 0.505
1.312AspMet: 1.312 ± 0.345
2.296AspAsn: 2.296 ± 0.641
1.64AspPro: 1.64 ± 0.471
1.421AspGln: 1.421 ± 0.472
2.187AspArg: 2.187 ± 0.546
2.296AspSer: 2.296 ± 0.407
3.936AspThr: 3.936 ± 0.756
3.826AspVal: 3.826 ± 0.678
0.765AspTrp: 0.765 ± 0.291
2.187AspTyr: 2.187 ± 0.499
0.0AspXaa: 0.0 ± 0.0
Glu
5.685GluAla: 5.685 ± 0.853
0.437GluCys: 0.437 ± 0.2
1.421GluAsp: 1.421 ± 0.474
2.733GluGlu: 2.733 ± 0.437
2.187GluPhe: 2.187 ± 0.55
2.952GluGly: 2.952 ± 0.529
1.64GluHis: 1.64 ± 0.519
3.826GluIle: 3.826 ± 0.723
4.045GluLys: 4.045 ± 0.676
7.653GluLeu: 7.653 ± 0.753
2.514GluMet: 2.514 ± 0.386
3.498GluAsn: 3.498 ± 0.73
2.624GluPro: 2.624 ± 0.436
2.187GluGln: 2.187 ± 0.539
4.701GluArg: 4.701 ± 0.976
4.264GluSer: 4.264 ± 0.566
2.624GluThr: 2.624 ± 0.583
2.952GluVal: 2.952 ± 0.537
0.656GluTrp: 0.656 ± 0.29
2.077GluTyr: 2.077 ± 0.555
0.0GluXaa: 0.0 ± 0.0
Phe
2.842PheAla: 2.842 ± 0.5
0.547PheCys: 0.547 ± 0.199
1.093PheAsp: 1.093 ± 0.313
2.514PheGlu: 2.514 ± 0.478
0.984PhePhe: 0.984 ± 0.39
1.749PheGly: 1.749 ± 0.409
1.203PheHis: 1.203 ± 0.311
1.312PheIle: 1.312 ± 0.366
2.405PheLys: 2.405 ± 0.526
2.733PheLeu: 2.733 ± 0.545
0.984PheMet: 0.984 ± 0.324
1.859PheAsn: 1.859 ± 0.4
1.203PhePro: 1.203 ± 0.345
1.64PheGln: 1.64 ± 0.337
1.421PheArg: 1.421 ± 0.388
2.952PheSer: 2.952 ± 0.595
2.405PheThr: 2.405 ± 0.52
1.421PheVal: 1.421 ± 0.373
0.765PheTrp: 0.765 ± 0.325
0.656PheTyr: 0.656 ± 0.316
0.0PheXaa: 0.0 ± 0.0
Gly
5.794GlyAla: 5.794 ± 0.89
0.875GlyCys: 0.875 ± 0.45
5.357GlyAsp: 5.357 ± 0.519
4.592GlyGlu: 4.592 ± 0.682
1.968GlyPhe: 1.968 ± 0.469
6.232GlyGly: 6.232 ± 1.367
0.875GlyHis: 0.875 ± 0.222
4.482GlyIle: 4.482 ± 0.812
4.482GlyLys: 4.482 ± 0.623
4.81GlyLeu: 4.81 ± 0.655
2.514GlyMet: 2.514 ± 0.563
2.733GlyAsn: 2.733 ± 0.522
0.547GlyPro: 0.547 ± 0.288
2.514GlyGln: 2.514 ± 0.522
4.482GlyArg: 4.482 ± 0.576
3.389GlySer: 3.389 ± 0.708
4.482GlyThr: 4.482 ± 0.821
5.685GlyVal: 5.685 ± 0.952
1.203GlyTrp: 1.203 ± 0.289
2.077GlyTyr: 2.077 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.531HisAla: 1.531 ± 0.472
0.437HisCys: 0.437 ± 0.23
1.203HisAsp: 1.203 ± 0.321
0.984HisGlu: 0.984 ± 0.299
0.437HisPhe: 0.437 ± 0.224
1.64HisGly: 1.64 ± 0.485
0.437HisHis: 0.437 ± 0.185
1.312HisIle: 1.312 ± 0.403
1.093HisLys: 1.093 ± 0.452
1.968HisLeu: 1.968 ± 0.361
0.765HisMet: 0.765 ± 0.296
0.875HisAsn: 0.875 ± 0.397
1.421HisPro: 1.421 ± 0.392
0.984HisGln: 0.984 ± 0.289
0.765HisArg: 0.765 ± 0.26
1.093HisSer: 1.093 ± 0.336
1.203HisThr: 1.203 ± 0.328
0.656HisVal: 0.656 ± 0.24
0.109HisTrp: 0.109 ± 0.101
0.656HisTyr: 0.656 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
5.138IleAla: 5.138 ± 0.698
0.109IleCys: 0.109 ± 0.132
4.373IleAsp: 4.373 ± 0.745
2.842IleGlu: 2.842 ± 0.408
1.859IlePhe: 1.859 ± 0.539
4.045IleGly: 4.045 ± 0.64
0.656IleHis: 0.656 ± 0.288
3.061IleIle: 3.061 ± 0.723
1.968IleLys: 1.968 ± 0.707
2.296IleLeu: 2.296 ± 0.535
0.875IleMet: 0.875 ± 0.292
2.296IleAsn: 2.296 ± 0.467
1.859IlePro: 1.859 ± 0.4
2.405IleGln: 2.405 ± 0.505
4.592IleArg: 4.592 ± 0.683
5.466IleSer: 5.466 ± 0.808
4.373IleThr: 4.373 ± 0.723
3.717IleVal: 3.717 ± 0.606
0.656IleTrp: 0.656 ± 0.234
1.093IleTyr: 1.093 ± 0.336
0.0IleXaa: 0.0 ± 0.0
Lys
6.669LysAla: 6.669 ± 0.976
0.109LysCys: 0.109 ± 0.114
1.64LysAsp: 1.64 ± 0.397
3.28LysGlu: 3.28 ± 0.647
1.421LysPhe: 1.421 ± 0.349
3.061LysGly: 3.061 ± 0.499
1.64LysHis: 1.64 ± 0.442
2.077LysIle: 2.077 ± 0.634
3.389LysLys: 3.389 ± 0.591
5.357LysLeu: 5.357 ± 0.849
1.093LysMet: 1.093 ± 0.426
2.952LysAsn: 2.952 ± 0.641
3.498LysPro: 3.498 ± 0.706
1.749LysGln: 1.749 ± 0.397
4.045LysArg: 4.045 ± 0.792
2.952LysSer: 2.952 ± 0.604
3.826LysThr: 3.826 ± 0.535
3.936LysVal: 3.936 ± 0.749
0.875LysTrp: 0.875 ± 0.351
2.624LysTyr: 2.624 ± 0.513
0.0LysXaa: 0.0 ± 0.0
Leu
9.074LeuAla: 9.074 ± 0.805
1.093LeuCys: 1.093 ± 0.464
4.92LeuAsp: 4.92 ± 0.801
4.482LeuGlu: 4.482 ± 0.712
3.826LeuPhe: 3.826 ± 0.878
5.248LeuGly: 5.248 ± 0.853
1.312LeuHis: 1.312 ± 0.299
3.826LeuIle: 3.826 ± 0.475
5.685LeuLys: 5.685 ± 0.747
7.106LeuLeu: 7.106 ± 0.975
2.733LeuMet: 2.733 ± 0.493
5.466LeuAsn: 5.466 ± 0.679
4.154LeuPro: 4.154 ± 0.714
3.498LeuGln: 3.498 ± 0.477
5.794LeuArg: 5.794 ± 0.87
8.09LeuSer: 8.09 ± 1.169
6.669LeuThr: 6.669 ± 1.292
3.826LeuVal: 3.826 ± 0.516
1.749LeuTrp: 1.749 ± 0.473
2.077LeuTyr: 2.077 ± 0.549
0.0LeuXaa: 0.0 ± 0.0
Met
3.061MetAla: 3.061 ± 0.556
0.109MetCys: 0.109 ± 0.12
0.765MetAsp: 0.765 ± 0.265
1.859MetGlu: 1.859 ± 0.363
1.421MetPhe: 1.421 ± 0.436
1.312MetGly: 1.312 ± 0.376
0.765MetHis: 0.765 ± 0.357
0.437MetIle: 0.437 ± 0.245
1.312MetLys: 1.312 ± 0.397
2.077MetLeu: 2.077 ± 0.494
0.765MetMet: 0.765 ± 0.315
1.859MetAsn: 1.859 ± 0.413
0.656MetPro: 0.656 ± 0.252
0.984MetGln: 0.984 ± 0.398
2.187MetArg: 2.187 ± 0.574
1.531MetSer: 1.531 ± 0.341
2.405MetThr: 2.405 ± 0.591
1.749MetVal: 1.749 ± 0.369
0.328MetTrp: 0.328 ± 0.198
0.547MetTyr: 0.547 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.264AsnAla: 4.264 ± 0.841
0.328AsnCys: 0.328 ± 0.167
2.187AsnAsp: 2.187 ± 0.556
2.624AsnGlu: 2.624 ± 0.55
1.203AsnPhe: 1.203 ± 0.363
3.826AsnGly: 3.826 ± 0.774
0.875AsnHis: 0.875 ± 0.318
2.624AsnIle: 2.624 ± 0.497
2.952AsnLys: 2.952 ± 0.463
3.17AsnLeu: 3.17 ± 0.525
0.547AsnMet: 0.547 ± 0.256
1.859AsnAsn: 1.859 ± 0.443
1.64AsnPro: 1.64 ± 0.398
1.312AsnGln: 1.312 ± 0.381
2.733AsnArg: 2.733 ± 0.604
2.624AsnSer: 2.624 ± 0.566
2.624AsnThr: 2.624 ± 0.504
2.187AsnVal: 2.187 ± 0.359
0.328AsnTrp: 0.328 ± 0.264
0.875AsnTyr: 0.875 ± 0.336
0.0AsnXaa: 0.0 ± 0.0
Pro
3.717ProAla: 3.717 ± 0.802
0.109ProCys: 0.109 ± 0.117
3.717ProAsp: 3.717 ± 0.811
3.717ProGlu: 3.717 ± 0.614
1.203ProPhe: 1.203 ± 0.561
2.296ProGly: 2.296 ± 0.547
1.203ProHis: 1.203 ± 0.422
2.077ProIle: 2.077 ± 0.49
2.296ProLys: 2.296 ± 0.562
4.373ProLeu: 4.373 ± 0.661
0.437ProMet: 0.437 ± 0.246
0.984ProAsn: 0.984 ± 0.37
1.531ProPro: 1.531 ± 0.321
1.312ProGln: 1.312 ± 0.418
2.733ProArg: 2.733 ± 0.856
1.968ProSer: 1.968 ± 0.579
1.531ProThr: 1.531 ± 0.298
5.029ProVal: 5.029 ± 0.698
0.328ProTrp: 0.328 ± 0.189
0.984ProTyr: 0.984 ± 0.396
0.0ProXaa: 0.0 ± 0.0
Gln
4.592GlnAla: 4.592 ± 1.199
0.328GlnCys: 0.328 ± 0.225
2.187GlnAsp: 2.187 ± 0.586
1.968GlnGlu: 1.968 ± 0.449
1.093GlnPhe: 1.093 ± 0.337
1.203GlnGly: 1.203 ± 0.283
0.656GlnHis: 0.656 ± 0.232
2.077GlnIle: 2.077 ± 0.613
2.624GlnLys: 2.624 ± 0.654
5.138GlnLeu: 5.138 ± 0.738
0.656GlnMet: 0.656 ± 0.302
0.984GlnAsn: 0.984 ± 0.314
1.312GlnPro: 1.312 ± 0.366
1.968GlnGln: 1.968 ± 0.537
4.264GlnArg: 4.264 ± 0.707
2.405GlnSer: 2.405 ± 0.607
2.952GlnThr: 2.952 ± 0.519
1.968GlnVal: 1.968 ± 0.536
0.547GlnTrp: 0.547 ± 0.346
0.984GlnTyr: 0.984 ± 0.342
0.0GlnXaa: 0.0 ± 0.0
Arg
5.904ArgAla: 5.904 ± 0.678
0.765ArgCys: 0.765 ± 0.303
3.28ArgAsp: 3.28 ± 0.618
4.373ArgGlu: 4.373 ± 0.653
1.749ArgPhe: 1.749 ± 0.447
3.498ArgGly: 3.498 ± 0.869
1.968ArgHis: 1.968 ± 0.398
3.389ArgIle: 3.389 ± 0.811
4.045ArgLys: 4.045 ± 0.56
6.232ArgLeu: 6.232 ± 0.903
1.312ArgMet: 1.312 ± 0.346
2.624ArgAsn: 2.624 ± 0.422
2.405ArgPro: 2.405 ± 0.483
2.952ArgGln: 2.952 ± 0.726
5.138ArgArg: 5.138 ± 0.915
3.28ArgSer: 3.28 ± 0.537
3.608ArgThr: 3.608 ± 0.465
4.92ArgVal: 4.92 ± 0.603
0.875ArgTrp: 0.875 ± 0.338
2.077ArgTyr: 2.077 ± 0.567
0.0ArgXaa: 0.0 ± 0.0
Ser
8.09SerAla: 8.09 ± 1.2
0.656SerCys: 0.656 ± 0.221
4.154SerAsp: 4.154 ± 0.65
4.045SerGlu: 4.045 ± 0.678
1.968SerPhe: 1.968 ± 0.508
5.357SerGly: 5.357 ± 0.651
0.984SerHis: 0.984 ± 0.468
2.733SerIle: 2.733 ± 0.59
3.28SerLys: 3.28 ± 0.521
5.357SerLeu: 5.357 ± 0.829
1.859SerMet: 1.859 ± 0.562
2.405SerAsn: 2.405 ± 0.516
2.514SerPro: 2.514 ± 0.594
2.733SerGln: 2.733 ± 0.361
3.936SerArg: 3.936 ± 0.723
1.859SerSer: 1.859 ± 0.411
2.952SerThr: 2.952 ± 0.541
4.701SerVal: 4.701 ± 0.683
0.547SerTrp: 0.547 ± 0.205
1.531SerTyr: 1.531 ± 0.443
0.0SerXaa: 0.0 ± 0.0
Thr
7.981ThrAla: 7.981 ± 1.25
0.765ThrCys: 0.765 ± 0.245
4.373ThrAsp: 4.373 ± 0.647
2.296ThrGlu: 2.296 ± 0.528
1.749ThrPhe: 1.749 ± 0.515
5.576ThrGly: 5.576 ± 0.714
0.984ThrHis: 0.984 ± 0.395
3.389ThrIle: 3.389 ± 0.542
2.733ThrLys: 2.733 ± 0.551
8.418ThrLeu: 8.418 ± 1.162
1.968ThrMet: 1.968 ± 0.397
1.312ThrAsn: 1.312 ± 0.33
2.733ThrPro: 2.733 ± 0.459
1.64ThrGln: 1.64 ± 0.494
3.28ThrArg: 3.28 ± 0.636
3.061ThrSer: 3.061 ± 0.568
3.608ThrThr: 3.608 ± 0.593
5.466ThrVal: 5.466 ± 0.868
0.765ThrTrp: 0.765 ± 0.219
1.531ThrTyr: 1.531 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
6.013ValAla: 6.013 ± 0.678
1.093ValCys: 1.093 ± 0.424
3.826ValAsp: 3.826 ± 0.575
5.248ValGlu: 5.248 ± 0.853
2.077ValPhe: 2.077 ± 0.529
4.154ValGly: 4.154 ± 0.807
0.656ValHis: 0.656 ± 0.228
4.482ValIle: 4.482 ± 0.831
4.154ValLys: 4.154 ± 0.563
5.029ValLeu: 5.029 ± 0.585
2.296ValMet: 2.296 ± 0.439
2.952ValAsn: 2.952 ± 0.574
3.389ValPro: 3.389 ± 0.613
2.624ValGln: 2.624 ± 0.601
2.624ValArg: 2.624 ± 0.478
4.482ValSer: 4.482 ± 0.653
4.92ValThr: 4.92 ± 0.655
5.029ValVal: 5.029 ± 0.833
0.656ValTrp: 0.656 ± 0.247
1.093ValTyr: 1.093 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
1.531TrpAla: 1.531 ± 0.323
0.0TrpCys: 0.0 ± 0.0
0.765TrpAsp: 0.765 ± 0.31
0.875TrpGlu: 0.875 ± 0.229
0.437TrpPhe: 0.437 ± 0.179
0.765TrpGly: 0.765 ± 0.331
0.765TrpHis: 0.765 ± 0.305
0.656TrpIle: 0.656 ± 0.253
0.547TrpLys: 0.547 ± 0.273
1.859TrpLeu: 1.859 ± 0.524
0.437TrpMet: 0.437 ± 0.218
0.547TrpAsn: 0.547 ± 0.314
0.984TrpPro: 0.984 ± 0.348
0.656TrpGln: 0.656 ± 0.224
1.312TrpArg: 1.312 ± 0.407
0.437TrpSer: 0.437 ± 0.207
0.328TrpThr: 0.328 ± 0.198
0.547TrpVal: 0.547 ± 0.262
0.547TrpTrp: 0.547 ± 0.22
0.437TrpTyr: 0.437 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.389TyrAla: 3.389 ± 0.699
0.219TyrCys: 0.219 ± 0.127
1.312TyrAsp: 1.312 ± 0.323
1.968TyrGlu: 1.968 ± 0.448
1.203TyrPhe: 1.203 ± 0.401
1.421TyrGly: 1.421 ± 0.357
0.547TyrHis: 0.547 ± 0.203
2.187TyrIle: 2.187 ± 0.479
0.765TyrLys: 0.765 ± 0.234
1.859TyrLeu: 1.859 ± 0.374
0.875TyrMet: 0.875 ± 0.254
0.765TyrAsn: 0.765 ± 0.258
1.312TyrPro: 1.312 ± 0.397
1.749TyrGln: 1.749 ± 0.418
1.859TyrArg: 1.859 ± 0.404
1.531TyrSer: 1.531 ± 0.389
1.64TyrThr: 1.64 ± 0.411
1.64TyrVal: 1.64 ± 0.442
0.656TyrTrp: 0.656 ± 0.265
0.875TyrTyr: 0.875 ± 0.352
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (9148 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski