Pseudogymnoascus sp. WSF 3629

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8CQI2|A0A1B8CQI2_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. WSF 3629 OX=1622147 GN=VE00_00111 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84GLLFLTLAANLGSAFTQVVDD22 pKa = 4.5LGLAASYY29 pKa = 10.83GVLAHH34 pKa = 7.05ASISNSGPTTVNGDD48 pKa = 2.81IGTTGTSIVGFPPGVYY64 pKa = 9.06TGNRR68 pKa = 11.84NVGLLATTAFNNAEE82 pKa = 3.94AAYY85 pKa = 6.44TTLGQLPSIILVGNLGGRR103 pKa = 11.84LLRR106 pKa = 11.84PGVYY110 pKa = 9.67RR111 pKa = 11.84FSTSAVLSGTLILAGQGSPCDD132 pKa = 3.0SWVFLIGSTLITSVGSSVLVTGGGNPGNVFWRR164 pKa = 11.84VGSSATIQIGSQFSGNILAGVAVTLNSGASIQGSVYY200 pKa = 10.73ALGSSVVLNSNEE212 pKa = 3.56VAAQEE217 pKa = 4.12NSCPLGASTSTSVSSTSTTASSDD240 pKa = 3.19SSTSTDD246 pKa = 2.67VSTTTDD252 pKa = 2.87SSTSTTTDD260 pKa = 2.7VTTTTSSDD268 pKa = 3.09SSTSTDD274 pKa = 2.69VSTTSTSTDD283 pKa = 2.96VPTTTDD289 pKa = 2.79SSTSTDD295 pKa = 2.9VSTTTSTSTDD305 pKa = 3.07VPTTTDD311 pKa = 2.79SSTSTDD317 pKa = 3.11VTTTTSTSTDD327 pKa = 3.07VPTTTDD333 pKa = 2.79SSTSTDD339 pKa = 3.11VTTTTSTSTDD349 pKa = 2.83VSTTDD354 pKa = 2.9VSTTTDD360 pKa = 2.62ITTATSTTTDD370 pKa = 2.97VTTATSTSTDD380 pKa = 2.74VSTTTDD386 pKa = 2.56ITTATDD392 pKa = 2.97VTTATDD398 pKa = 2.92ITTATNITTAIDD410 pKa = 3.05ITTATDD416 pKa = 2.65ITTATDD422 pKa = 2.72ITTATDD428 pKa = 2.97VTTATDD434 pKa = 3.04ITTATSTSTDD444 pKa = 3.08VTTTTSTSTDD454 pKa = 3.07VPTTTDD460 pKa = 3.03SSSSSSSVTSASSSLTSTSMASLPSLTSSTIISVTNSSAVSSPPASTTSTVYY512 pKa = 9.67ATTVYY517 pKa = 10.51TITKK521 pKa = 10.05CPATVTNCPIGSTTTEE537 pKa = 4.72LISLYY542 pKa = 8.45TTVCPVAASATKK554 pKa = 10.05QPPSGYY560 pKa = 8.25TVSTVFTTIVYY571 pKa = 8.07TITKK575 pKa = 9.52CPKK578 pKa = 9.13TVTNCPVGSVTTEE591 pKa = 3.17IRR593 pKa = 11.84SLYY596 pKa = 7.48TTTCPVTQSFVNSVLPPQSSIPARR620 pKa = 11.84PASSQLPAAVLPSRR634 pKa = 11.84ISSIAIASPVSTVSPALGLSGAATVSSGAAPVSSVSPASGPSGVPGSVPLIGAPPSSNLVISTVTASKK702 pKa = 9.29GTANVSQTSQPSAAYY717 pKa = 7.41TGPIASSGVMNKK729 pKa = 8.04GTSASLLVLAIGALLFLL746 pKa = 5.21

Molecular weight: 73.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8CG56|A0A1B8CG56_9PEZI Glucanase OS=Pseudogymnoascus sp. WSF 3629 OX=1622147 GN=VE00_03807 PE=3 SV=1
MM1 pKa = 7.4FSLRR5 pKa = 11.84SSRR8 pKa = 11.84GVTAALKK15 pKa = 10.29PVMQARR21 pKa = 11.84AVKK24 pKa = 10.25SVAPQASRR32 pKa = 11.84TFSILTPLRR41 pKa = 11.84PSLTSSFAPRR51 pKa = 11.84ASAALDD57 pKa = 3.58TTTTTSTTTSAAGTTTILDD76 pKa = 4.59LLPKK80 pKa = 10.19ISTHH84 pKa = 6.55PSLAGIQVRR93 pKa = 11.84CGPRR97 pKa = 11.84NTFSPSHH104 pKa = 6.08FVRR107 pKa = 11.84KK108 pKa = 9.24RR109 pKa = 11.84RR110 pKa = 11.84HH111 pKa = 5.18GFLSRR116 pKa = 11.84VRR118 pKa = 11.84TRR120 pKa = 11.84KK121 pKa = 9.59GRR123 pKa = 11.84ATLQRR128 pKa = 11.84RR129 pKa = 11.84KK130 pKa = 10.21SKK132 pKa = 10.49NRR134 pKa = 11.84STLSHH139 pKa = 6.67

Molecular weight: 15.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11000

0

11000

5350055

51

5659

486.4

53.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.936 ± 0.022

1.161 ± 0.01

5.608 ± 0.014

6.355 ± 0.023

3.64 ± 0.013

7.306 ± 0.023

2.213 ± 0.01

5.02 ± 0.017

5.013 ± 0.021

8.769 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.008

3.709 ± 0.014

5.95 ± 0.022

3.825 ± 0.018

5.819 ± 0.022

8.072 ± 0.023

6.075 ± 0.016

6.187 ± 0.02

1.412 ± 0.008

2.748 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski