Populus alba (White poplar)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U5QPE4|A0A4U5QPE4_POPAL Phenolic glucoside malonyltransferase 1-like OS=Populus alba OX=43335 GN=D5086_0000061100 PE=3 SV=1
MM1 pKa = 7.48SFLFIFSGAHH11 pKa = 4.1TFGRR15 pKa = 11.84AKK17 pKa = 10.6CSTFDD22 pKa = 3.62FRR24 pKa = 11.84LYY26 pKa = 11.02DD27 pKa = 3.96FNSTGAPDD35 pKa = 4.2PSLDD39 pKa = 3.7PTLLAALQEE48 pKa = 4.27LCPQGGNGSVLTDD61 pKa = 4.62LDD63 pKa = 4.21LTTPDD68 pKa = 4.55AFDD71 pKa = 3.55SNYY74 pKa = 10.44YY75 pKa = 11.04SNLQGNQGLLQTDD88 pKa = 3.46QVLFSTPGADD98 pKa = 5.42DD99 pKa = 4.88IIALVNAFSANQTAFFEE116 pKa = 4.71SFAEE120 pKa = 4.14SMIRR124 pKa = 11.84MGNLSPLTGTEE135 pKa = 3.86GEE137 pKa = 4.02IRR139 pKa = 11.84LNCSVVNANLAGPDD153 pKa = 3.48SMLVSSII160 pKa = 3.83

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U5QCJ3|A0A4U5QCJ3_POPAL LysM domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000121300 PE=4 SV=1
MM1 pKa = 7.65IFLDD5 pKa = 5.04LGALGLWGSGFRR17 pKa = 11.84GFGVSGLGFRR27 pKa = 11.84VSGFRR32 pKa = 11.84VQGSGFRR39 pKa = 11.84VQGLRR44 pKa = 11.84VQDD47 pKa = 3.28LGFRR51 pKa = 11.84VSGFRR56 pKa = 11.84VSGFRR61 pKa = 11.84VRR63 pKa = 11.84GSGFRR68 pKa = 11.84VQGSGFRR75 pKa = 11.84VQGSGFRR82 pKa = 11.84VLGFRR87 pKa = 11.84VQGFGGRR94 pKa = 11.84GLGFRR99 pKa = 11.84GFGVWGFGFGVWGFRR114 pKa = 11.84VWGLGFGIWGFGVWDD129 pKa = 4.34LGFGVWGLGFGVWGFGVSGFRR150 pKa = 11.84GLGFRR155 pKa = 11.84VV156 pKa = 3.28

Molecular weight: 16.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32718

0

32718

13899524

54

6213

424.8

47.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.765 ± 0.014

1.855 ± 0.007

5.286 ± 0.009

6.462 ± 0.015

4.167 ± 0.009

6.687 ± 0.015

2.379 ± 0.005

5.202 ± 0.01

6.081 ± 0.013

9.714 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.005

4.489 ± 0.009

5.019 ± 0.016

3.759 ± 0.01

5.267 ± 0.011

9.161 ± 0.018

4.893 ± 0.008

6.432 ± 0.009

1.266 ± 0.005

2.713 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski