Tenacibaculum mesophilum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5GT67|A0A1M5GT67_9FLAO Ribosomal large subunit pseudouridine synthase D OS=Tenacibaculum mesophilum OX=104268 GN=SAMN05444344_2568 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.06NKK4 pKa = 9.64HH5 pKa = 5.23PHH7 pKa = 5.01YY8 pKa = 10.37SVYY11 pKa = 10.83KK12 pKa = 9.19SILYY16 pKa = 10.07IIFLTFTISLSSQVIIKK33 pKa = 10.47EE34 pKa = 3.85EE35 pKa = 4.04LKK37 pKa = 10.7RR38 pKa = 11.84SNLNKK43 pKa = 9.15TLSYY47 pKa = 10.59KK48 pKa = 10.45PPQDD52 pKa = 3.44TKK54 pKa = 11.23LSSSNNLVNPISSSFLTKK72 pKa = 10.53KK73 pKa = 8.59ITNSNLNQRR82 pKa = 11.84SFSSTFSMMMMATPTLDD99 pKa = 3.95LNSITPGNNYY109 pKa = 9.99DD110 pKa = 3.84FQLQPTTSNIFPVTVDD126 pKa = 3.52PKK128 pKa = 9.35VTTDD132 pKa = 3.28TGVLSATISFSGIVDD147 pKa = 3.76TPFGEE152 pKa = 4.69LLAINNGGGFDD163 pKa = 4.82LLWLQLPVSGPFTYY177 pKa = 10.07TIGSSTIQITQLTNTTFNIVEE198 pKa = 4.26TSGNPISNSEE208 pKa = 4.13FEE210 pKa = 4.7TFLSTLFYY218 pKa = 11.37GDD220 pKa = 3.94LQSPYY225 pKa = 8.63TDD227 pKa = 3.25GVRR230 pKa = 11.84TMDD233 pKa = 3.18VTIFDD238 pKa = 4.35TNGDD242 pKa = 3.77STSAQTIIRR251 pKa = 11.84IYY253 pKa = 6.69TTPPTVVDD261 pKa = 3.74EE262 pKa = 4.86ADD264 pKa = 3.91SILANSTGTITGNVLTNDD282 pKa = 3.66SGSTAISVTEE292 pKa = 3.72VDD294 pKa = 4.23VYY296 pKa = 10.95PSAVNNTYY304 pKa = 7.33TTLYY308 pKa = 11.05GDD310 pKa = 3.46ITIQANGTYY319 pKa = 9.49TYY321 pKa = 11.28DD322 pKa = 3.38VDD324 pKa = 3.71EE325 pKa = 4.87TNASVTGLRR334 pKa = 11.84NGEE337 pKa = 4.18SLDD340 pKa = 4.84DD341 pKa = 4.48IISYY345 pKa = 8.56TVADD349 pKa = 4.74AIGIKK354 pKa = 10.21DD355 pKa = 3.82YY356 pKa = 11.58GILTITINGVDD367 pKa = 4.23EE368 pKa = 5.11APDD371 pKa = 4.97AIDD374 pKa = 3.59NTDD377 pKa = 3.39SLTAFIDD384 pKa = 3.97ASATGNVITDD394 pKa = 4.2PGTGGSDD401 pKa = 3.1TVDD404 pKa = 3.12RR405 pKa = 11.84GLSTLVWEE413 pKa = 4.93SNFTNGEE420 pKa = 4.34TVNGKK425 pKa = 7.69TKK427 pKa = 10.21TIGGVGLSFTTLDD440 pKa = 3.39PGGFGTSFNQTVDD453 pKa = 3.26YY454 pKa = 7.14TTNGGHH460 pKa = 6.07TGYY463 pKa = 10.79LIYY466 pKa = 11.05NIDD469 pKa = 3.73GTTNPTDD476 pKa = 3.52DD477 pKa = 3.81TVLVIDD483 pKa = 4.49FDD485 pKa = 4.1QPVFNLGFLLTDD497 pKa = 3.35IDD499 pKa = 4.78FSQGSSWQDD508 pKa = 3.14QIKK511 pKa = 10.47IEE513 pKa = 4.24GNLDD517 pKa = 4.21GINSTYY523 pKa = 11.06KK524 pKa = 10.13FVTTGGVINSGSNTFYY540 pKa = 11.14GIGSAIPSDD549 pKa = 3.26ATGNINVFFEE559 pKa = 4.1QPINQLRR566 pKa = 11.84LSYY569 pKa = 10.96NYY571 pKa = 9.82GPNVTDD577 pKa = 5.05PDD579 pKa = 4.24PLGQIAGVSDD589 pKa = 4.53IYY591 pKa = 10.36WQGSGSVTTIRR602 pKa = 11.84LGTTLGNLNAGNLNILYY619 pKa = 8.94PGTYY623 pKa = 10.17GSIIMNPDD631 pKa = 2.44GSYY634 pKa = 10.7EE635 pKa = 4.26YY636 pKa = 10.35IVDD639 pKa = 3.91TSNPAVAGLLVGQTLTDD656 pKa = 3.35TFFYY660 pKa = 10.28EE661 pKa = 3.86ISDD664 pKa = 3.86GVSSDD669 pKa = 3.22TANLVITINGSGTDD683 pKa = 3.64PDD685 pKa = 4.21GDD687 pKa = 4.36GIADD691 pKa = 4.81RR692 pKa = 11.84LDD694 pKa = 4.55LDD696 pKa = 4.95DD697 pKa = 6.5DD698 pKa = 4.28NDD700 pKa = 5.04GILDD704 pKa = 3.96TEE706 pKa = 4.45EE707 pKa = 4.54SLACAGTPLDD717 pKa = 3.83LTGIGTTLTSGSYY730 pKa = 10.45FDD732 pKa = 4.74QDD734 pKa = 3.39TEE736 pKa = 4.55KK737 pKa = 10.68IIDD740 pKa = 3.66VTITTTGTVDD750 pKa = 2.93EE751 pKa = 5.33GNANGDD757 pKa = 3.49ILITQGGTATFTFSSPVTVSLKK779 pKa = 10.7HH780 pKa = 6.14EE781 pKa = 4.2IGITGNFDD789 pKa = 3.71TGDD792 pKa = 3.46TWEE795 pKa = 4.3LTSTGEE801 pKa = 4.13FTVADD806 pKa = 4.12PNGDD810 pKa = 3.6LMINSNSGGILNFDD824 pKa = 3.6ALGAISASEE833 pKa = 3.57AWEE836 pKa = 3.96IKK838 pKa = 7.81TTTTSLKK845 pKa = 10.65LDD847 pKa = 4.01LKK849 pKa = 10.96SGNTKK854 pKa = 10.53SPINLALICGGFLDD868 pKa = 4.82SDD870 pKa = 3.64NDD872 pKa = 4.73GIPNHH877 pKa = 7.09LDD879 pKa = 3.34LDD881 pKa = 4.22SDD883 pKa = 4.06NDD885 pKa = 3.96GCFDD889 pKa = 3.85AVEE892 pKa = 4.11ASEE895 pKa = 4.36NVSSGQLDD903 pKa = 3.71GG904 pKa = 4.71

Molecular weight: 95.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5H9J5|A0A1M5H9J5_9FLAO Uncharacterized protein OS=Tenacibaculum mesophilum OX=104268 GN=SAMN05444344_2819 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.52FIDD5 pKa = 3.93FVRR8 pKa = 11.84LFFEE12 pKa = 3.75RR13 pKa = 11.84HH14 pKa = 4.78GFNVSSRR21 pKa = 11.84FADD24 pKa = 3.65RR25 pKa = 11.84LGMRR29 pKa = 11.84ATNVRR34 pKa = 11.84LFFIYY39 pKa = 10.25ISFVTVGLSFGLYY52 pKa = 8.77LTMAFLLRR60 pKa = 11.84LKK62 pKa = 11.1DD63 pKa = 3.36MIYY66 pKa = 10.24TKK68 pKa = 9.83RR69 pKa = 11.84TSVFDD74 pKa = 3.66LL75 pKa = 4.18

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2966

0

2966

989990

31

6017

333.8

37.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.0 ± 0.049

0.719 ± 0.016

5.294 ± 0.036

6.859 ± 0.05

5.2 ± 0.039

6.173 ± 0.051

1.694 ± 0.023

7.995 ± 0.045

8.621 ± 0.067

9.132 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.008 ± 0.022

6.557 ± 0.051

3.205 ± 0.028

3.359 ± 0.027

3.214 ± 0.029

6.431 ± 0.038

6.089 ± 0.064

6.329 ± 0.038

0.989 ± 0.017

4.13 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski