Amino acid dipepetide frequency for Streptococcus phage phi3396

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.737AlaAla: 3.737 ± 1.206
0.332AlaCys: 0.332 ± 0.172
3.571AlaAsp: 3.571 ± 0.445
5.315AlaGlu: 5.315 ± 0.929
2.159AlaPhe: 2.159 ± 0.539
3.405AlaGly: 3.405 ± 0.741
0.83AlaHis: 0.83 ± 0.292
4.401AlaIle: 4.401 ± 0.473
7.391AlaLys: 7.391 ± 0.999
5.73AlaLeu: 5.73 ± 1.225
1.827AlaMet: 1.827 ± 0.625
4.9AlaAsn: 4.9 ± 0.829
1.329AlaPro: 1.329 ± 0.399
3.903AlaGln: 3.903 ± 0.81
2.906AlaArg: 2.906 ± 0.424
4.733AlaSer: 4.733 ± 1.307
3.073AlaThr: 3.073 ± 0.527
3.82AlaVal: 3.82 ± 0.624
0.581AlaTrp: 0.581 ± 0.211
2.491AlaTyr: 2.491 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
0.166CysAla: 0.166 ± 0.118
0.083CysCys: 0.083 ± 0.082
0.249CysAsp: 0.249 ± 0.156
0.913CysGlu: 0.913 ± 0.344
0.083CysPhe: 0.083 ± 0.084
0.664CysGly: 0.664 ± 0.291
0.083CysHis: 0.083 ± 0.085
1.08CysIle: 1.08 ± 0.348
0.581CysLys: 0.581 ± 0.245
0.166CysLeu: 0.166 ± 0.101
0.249CysMet: 0.249 ± 0.152
0.249CysAsn: 0.249 ± 0.159
0.249CysPro: 0.249 ± 0.148
0.083CysGln: 0.083 ± 0.095
0.332CysArg: 0.332 ± 0.174
0.415CysSer: 0.415 ± 0.237
0.083CysThr: 0.083 ± 0.09
0.249CysVal: 0.249 ± 0.159
0.0CysTrp: 0.0 ± 0.0
0.498CysTyr: 0.498 ± 0.245
0.0CysXaa: 0.0 ± 0.0
Asp
4.069AspAla: 4.069 ± 0.595
0.83AspCys: 0.83 ± 0.292
3.903AspAsp: 3.903 ± 0.645
4.816AspGlu: 4.816 ± 0.505
2.823AspPhe: 2.823 ± 0.524
4.733AspGly: 4.733 ± 0.912
0.415AspHis: 0.415 ± 0.153
4.318AspIle: 4.318 ± 0.672
6.893AspLys: 6.893 ± 0.651
5.979AspLeu: 5.979 ± 0.731
2.408AspMet: 2.408 ± 0.431
3.488AspAsn: 3.488 ± 0.482
1.246AspPro: 1.246 ± 0.286
2.325AspGln: 2.325 ± 0.348
1.91AspArg: 1.91 ± 0.354
3.571AspSer: 3.571 ± 0.451
3.405AspThr: 3.405 ± 0.529
3.073AspVal: 3.073 ± 0.466
1.412AspTrp: 1.412 ± 0.346
3.488AspTyr: 3.488 ± 0.661
0.0AspXaa: 0.0 ± 0.0
Glu
5.398GluAla: 5.398 ± 0.987
0.332GluCys: 0.332 ± 0.2
3.156GluAsp: 3.156 ± 0.52
6.976GluGlu: 6.976 ± 1.026
3.405GluPhe: 3.405 ± 0.592
3.405GluGly: 3.405 ± 0.607
0.498GluHis: 0.498 ± 0.184
7.142GluIle: 7.142 ± 0.841
7.723GluLys: 7.723 ± 0.74
9.301GluLeu: 9.301 ± 0.771
1.661GluMet: 1.661 ± 0.387
2.491GluAsn: 2.491 ± 0.403
1.993GluPro: 1.993 ± 0.438
3.073GluGln: 3.073 ± 0.671
3.322GluArg: 3.322 ± 0.458
5.066GluSer: 5.066 ± 0.506
4.235GluThr: 4.235 ± 0.592
5.398GluVal: 5.398 ± 0.707
0.997GluTrp: 0.997 ± 0.276
2.159GluTyr: 2.159 ± 0.582
0.0GluXaa: 0.0 ± 0.0
Phe
1.91PheAla: 1.91 ± 0.402
0.166PheCys: 0.166 ± 0.126
3.322PheAsp: 3.322 ± 0.515
3.488PheGlu: 3.488 ± 0.625
1.993PhePhe: 1.993 ± 0.562
2.906PheGly: 2.906 ± 0.502
0.913PheHis: 0.913 ± 0.307
2.574PheIle: 2.574 ± 0.492
3.737PheLys: 3.737 ± 0.564
2.491PheLeu: 2.491 ± 0.467
1.08PheMet: 1.08 ± 0.237
2.325PheAsn: 2.325 ± 0.488
0.664PhePro: 0.664 ± 0.254
0.913PheGln: 0.913 ± 0.305
2.076PheArg: 2.076 ± 0.414
2.159PheSer: 2.159 ± 0.478
2.325PheThr: 2.325 ± 0.389
2.906PheVal: 2.906 ± 0.517
0.415PheTrp: 0.415 ± 0.19
1.246PheTyr: 1.246 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
2.74GlyAla: 2.74 ± 0.573
0.332GlyCys: 0.332 ± 0.161
3.737GlyAsp: 3.737 ± 0.743
4.152GlyGlu: 4.152 ± 0.592
2.242GlyPhe: 2.242 ± 0.482
2.99GlyGly: 2.99 ± 0.473
0.997GlyHis: 0.997 ± 0.235
4.484GlyIle: 4.484 ± 0.536
5.979GlyLys: 5.979 ± 0.662
6.145GlyLeu: 6.145 ± 0.947
1.827GlyMet: 1.827 ± 0.325
3.322GlyAsn: 3.322 ± 0.534
1.163GlyPro: 1.163 ± 0.273
2.906GlyGln: 2.906 ± 0.556
3.156GlyArg: 3.156 ± 0.454
2.408GlySer: 2.408 ± 0.535
3.239GlyThr: 3.239 ± 0.664
3.073GlyVal: 3.073 ± 0.619
0.913GlyTrp: 0.913 ± 0.311
2.491GlyTyr: 2.491 ± 0.571
0.0GlyXaa: 0.0 ± 0.0
His
0.249HisAla: 0.249 ± 0.17
0.0HisCys: 0.0 ± 0.0
0.997HisAsp: 0.997 ± 0.361
1.246HisGlu: 1.246 ± 0.368
0.664HisPhe: 0.664 ± 0.209
1.08HisGly: 1.08 ± 0.205
0.332HisHis: 0.332 ± 0.177
0.581HisIle: 0.581 ± 0.235
0.747HisLys: 0.747 ± 0.233
0.83HisLeu: 0.83 ± 0.216
0.581HisMet: 0.581 ± 0.206
0.83HisAsn: 0.83 ± 0.251
0.581HisPro: 0.581 ± 0.222
1.08HisGln: 1.08 ± 0.311
0.498HisArg: 0.498 ± 0.198
0.581HisSer: 0.581 ± 0.217
0.664HisThr: 0.664 ± 0.235
0.997HisVal: 0.997 ± 0.321
0.166HisTrp: 0.166 ± 0.109
0.415HisTyr: 0.415 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.398IleAla: 5.398 ± 0.517
0.498IleCys: 0.498 ± 0.191
5.315IleAsp: 5.315 ± 0.773
6.81IleGlu: 6.81 ± 0.681
2.823IlePhe: 2.823 ± 0.597
3.903IleGly: 3.903 ± 0.525
0.913IleHis: 0.913 ± 0.306
3.571IleIle: 3.571 ± 0.528
8.221IleLys: 8.221 ± 0.978
4.235IleLeu: 4.235 ± 0.791
1.08IleMet: 1.08 ± 0.303
3.986IleAsn: 3.986 ± 0.427
2.242IlePro: 2.242 ± 0.457
1.08IleGln: 1.08 ± 0.265
2.823IleArg: 2.823 ± 0.418
3.986IleSer: 3.986 ± 0.517
5.066IleThr: 5.066 ± 0.64
3.654IleVal: 3.654 ± 0.435
0.415IleTrp: 0.415 ± 0.203
2.906IleTyr: 2.906 ± 0.496
0.0IleXaa: 0.0 ± 0.0
Lys
7.142LysAla: 7.142 ± 1.042
0.664LysCys: 0.664 ± 0.237
7.059LysAsp: 7.059 ± 0.698
8.055LysGlu: 8.055 ± 0.754
2.99LysPhe: 2.99 ± 0.549
5.73LysGly: 5.73 ± 0.872
0.913LysHis: 0.913 ± 0.251
7.225LysIle: 7.225 ± 0.794
7.474LysLys: 7.474 ± 0.837
8.387LysLeu: 8.387 ± 0.787
3.073LysMet: 3.073 ± 0.429
6.311LysAsn: 6.311 ± 0.678
2.076LysPro: 2.076 ± 0.555
4.235LysGln: 4.235 ± 0.862
4.484LysArg: 4.484 ± 0.671
5.149LysSer: 5.149 ± 0.69
5.398LysThr: 5.398 ± 0.679
6.311LysVal: 6.311 ± 0.631
1.08LysTrp: 1.08 ± 0.311
3.322LysTyr: 3.322 ± 0.504
0.0LysXaa: 0.0 ± 0.0
Leu
6.893LeuAla: 6.893 ± 1.179
0.415LeuCys: 0.415 ± 0.17
6.56LeuAsp: 6.56 ± 0.675
7.723LeuGlu: 7.723 ± 1.018
2.657LeuPhe: 2.657 ± 0.48
5.066LeuGly: 5.066 ± 0.909
0.83LeuHis: 0.83 ± 0.284
4.484LeuIle: 4.484 ± 0.469
9.467LeuLys: 9.467 ± 0.799
6.976LeuLeu: 6.976 ± 0.792
1.91LeuMet: 1.91 ± 0.469
4.9LeuAsn: 4.9 ± 0.847
2.657LeuPro: 2.657 ± 0.445
3.571LeuGln: 3.571 ± 0.561
3.073LeuArg: 3.073 ± 0.559
6.56LeuSer: 6.56 ± 1.006
6.643LeuThr: 6.643 ± 0.761
4.069LeuVal: 4.069 ± 0.543
0.664LeuTrp: 0.664 ± 0.207
2.491LeuTyr: 2.491 ± 0.543
0.0LeuXaa: 0.0 ± 0.0
Met
1.163MetAla: 1.163 ± 0.352
0.083MetCys: 0.083 ± 0.076
1.246MetAsp: 1.246 ± 0.363
2.325MetGlu: 2.325 ± 0.474
1.163MetPhe: 1.163 ± 0.274
1.163MetGly: 1.163 ± 0.224
0.083MetHis: 0.083 ± 0.095
2.823MetIle: 2.823 ± 0.497
1.827MetLys: 1.827 ± 0.484
2.74MetLeu: 2.74 ± 0.551
1.246MetMet: 1.246 ± 0.369
0.83MetAsn: 0.83 ± 0.281
0.581MetPro: 0.581 ± 0.228
0.747MetGln: 0.747 ± 0.269
1.661MetArg: 1.661 ± 0.458
1.827MetSer: 1.827 ± 0.35
2.242MetThr: 2.242 ± 0.437
1.495MetVal: 1.495 ± 0.304
0.415MetTrp: 0.415 ± 0.19
0.747MetTyr: 0.747 ± 0.295
0.0MetXaa: 0.0 ± 0.0
Asn
3.654AsnAla: 3.654 ± 1.245
0.581AsnCys: 0.581 ± 0.212
3.488AsnAsp: 3.488 ± 0.583
2.491AsnGlu: 2.491 ± 0.466
2.242AsnPhe: 2.242 ± 0.491
3.82AsnGly: 3.82 ± 0.557
1.08AsnHis: 1.08 ± 0.292
2.823AsnIle: 2.823 ± 0.579
4.816AsnLys: 4.816 ± 0.656
5.149AsnLeu: 5.149 ± 0.787
2.242AsnMet: 2.242 ± 0.433
3.322AsnAsn: 3.322 ± 0.691
2.159AsnPro: 2.159 ± 0.431
2.823AsnGln: 2.823 ± 0.631
2.574AsnArg: 2.574 ± 0.481
2.823AsnSer: 2.823 ± 0.4
3.322AsnThr: 3.322 ± 0.469
2.906AsnVal: 2.906 ± 0.568
0.913AsnTrp: 0.913 ± 0.296
3.073AsnTyr: 3.073 ± 0.631
0.0AsnXaa: 0.0 ± 0.0
Pro
1.412ProAla: 1.412 ± 0.286
0.083ProCys: 0.083 ± 0.086
1.744ProAsp: 1.744 ± 0.367
2.159ProGlu: 2.159 ± 0.383
1.08ProPhe: 1.08 ± 0.25
0.913ProGly: 0.913 ± 0.265
0.332ProHis: 0.332 ± 0.162
1.744ProIle: 1.744 ± 0.355
2.657ProLys: 2.657 ± 0.472
1.91ProLeu: 1.91 ± 0.412
0.581ProMet: 0.581 ± 0.264
1.329ProAsn: 1.329 ± 0.374
0.83ProPro: 0.83 ± 0.328
0.997ProGln: 0.997 ± 0.251
1.246ProArg: 1.246 ± 0.361
1.578ProSer: 1.578 ± 0.412
1.163ProThr: 1.163 ± 0.282
1.412ProVal: 1.412 ± 0.363
0.166ProTrp: 0.166 ± 0.123
0.664ProTyr: 0.664 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
2.99GlnAla: 2.99 ± 0.702
0.249GlnCys: 0.249 ± 0.153
1.91GlnAsp: 1.91 ± 0.402
2.408GlnGlu: 2.408 ± 0.393
1.412GlnPhe: 1.412 ± 0.433
1.91GlnGly: 1.91 ± 0.504
0.249GlnHis: 0.249 ± 0.123
2.906GlnIle: 2.906 ± 0.53
5.232GlnLys: 5.232 ± 0.81
4.484GlnLeu: 4.484 ± 0.681
0.83GlnMet: 0.83 ± 0.324
2.408GlnAsn: 2.408 ± 0.426
0.581GlnPro: 0.581 ± 0.262
1.578GlnGln: 1.578 ± 0.298
1.744GlnArg: 1.744 ± 0.388
3.654GlnSer: 3.654 ± 0.542
2.74GlnThr: 2.74 ± 0.428
1.91GlnVal: 1.91 ± 0.433
0.166GlnTrp: 0.166 ± 0.133
1.661GlnTyr: 1.661 ± 0.46
0.0GlnXaa: 0.0 ± 0.0
Arg
2.99ArgAla: 2.99 ± 0.501
0.498ArgCys: 0.498 ± 0.205
2.99ArgAsp: 2.99 ± 0.502
2.99ArgGlu: 2.99 ± 0.625
1.412ArgPhe: 1.412 ± 0.225
2.491ArgGly: 2.491 ± 0.471
0.997ArgHis: 0.997 ± 0.263
3.488ArgIle: 3.488 ± 0.553
3.488ArgLys: 3.488 ± 0.548
4.484ArgLeu: 4.484 ± 0.584
1.08ArgMet: 1.08 ± 0.294
2.408ArgAsn: 2.408 ± 0.403
0.747ArgPro: 0.747 ± 0.255
2.076ArgGln: 2.076 ± 0.388
2.906ArgArg: 2.906 ± 0.639
1.495ArgSer: 1.495 ± 0.312
1.827ArgThr: 1.827 ± 0.441
2.242ArgVal: 2.242 ± 0.337
1.08ArgTrp: 1.08 ± 0.298
2.657ArgTyr: 2.657 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
4.401SerAla: 4.401 ± 0.765
0.332SerCys: 0.332 ± 0.2
4.484SerAsp: 4.484 ± 0.699
4.318SerGlu: 4.318 ± 0.667
2.491SerPhe: 2.491 ± 0.434
3.737SerGly: 3.737 ± 0.731
0.747SerHis: 0.747 ± 0.236
4.152SerIle: 4.152 ± 0.618
4.816SerLys: 4.816 ± 0.803
5.232SerLeu: 5.232 ± 0.843
1.163SerMet: 1.163 ± 0.299
3.654SerAsn: 3.654 ± 0.598
1.661SerPro: 1.661 ± 0.435
2.408SerGln: 2.408 ± 0.454
3.073SerArg: 3.073 ± 0.561
4.152SerSer: 4.152 ± 1.216
3.571SerThr: 3.571 ± 0.508
4.069SerVal: 4.069 ± 0.608
0.415SerTrp: 0.415 ± 0.233
1.661SerTyr: 1.661 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.65ThrAla: 4.65 ± 1.075
0.249ThrCys: 0.249 ± 0.168
2.906ThrAsp: 2.906 ± 0.403
3.405ThrGlu: 3.405 ± 0.577
2.657ThrPhe: 2.657 ± 0.583
4.567ThrGly: 4.567 ± 0.704
0.747ThrHis: 0.747 ± 0.206
3.737ThrIle: 3.737 ± 0.539
5.73ThrLys: 5.73 ± 0.771
4.567ThrLeu: 4.567 ± 0.473
0.83ThrMet: 0.83 ± 0.302
3.156ThrAsn: 3.156 ± 0.544
1.163ThrPro: 1.163 ± 0.272
2.906ThrGln: 2.906 ± 0.481
1.495ThrArg: 1.495 ± 0.355
4.484ThrSer: 4.484 ± 0.593
3.405ThrThr: 3.405 ± 0.561
3.654ThrVal: 3.654 ± 0.529
0.747ThrTrp: 0.747 ± 0.204
2.408ThrTyr: 2.408 ± 0.503
0.0ThrXaa: 0.0 ± 0.0
Val
4.816ValAla: 4.816 ± 0.652
0.332ValCys: 0.332 ± 0.208
4.983ValAsp: 4.983 ± 0.532
4.401ValGlu: 4.401 ± 0.602
2.574ValPhe: 2.574 ± 0.438
2.657ValGly: 2.657 ± 0.392
0.747ValHis: 0.747 ± 0.239
3.82ValIle: 3.82 ± 0.561
5.647ValLys: 5.647 ± 0.565
3.654ValLeu: 3.654 ± 0.501
1.412ValMet: 1.412 ± 0.327
2.906ValAsn: 2.906 ± 0.522
1.163ValPro: 1.163 ± 0.293
1.993ValGln: 1.993 ± 0.416
2.906ValArg: 2.906 ± 0.438
3.571ValSer: 3.571 ± 0.447
3.737ValThr: 3.737 ± 0.673
4.069ValVal: 4.069 ± 0.528
0.747ValTrp: 0.747 ± 0.224
2.408ValTyr: 2.408 ± 0.588
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.179
0.166TrpCys: 0.166 ± 0.106
0.83TrpAsp: 0.83 ± 0.237
0.997TrpGlu: 0.997 ± 0.223
0.664TrpPhe: 0.664 ± 0.248
0.747TrpGly: 0.747 ± 0.214
0.332TrpHis: 0.332 ± 0.151
0.997TrpIle: 0.997 ± 0.293
1.329TrpLys: 1.329 ± 0.376
1.661TrpLeu: 1.661 ± 0.512
0.249TrpMet: 0.249 ± 0.131
0.913TrpAsn: 0.913 ± 0.385
0.0TrpPro: 0.0 ± 0.0
0.581TrpGln: 0.581 ± 0.231
0.332TrpArg: 0.332 ± 0.172
0.498TrpSer: 0.498 ± 0.163
0.249TrpThr: 0.249 ± 0.175
0.415TrpVal: 0.415 ± 0.206
0.083TrpTrp: 0.083 ± 0.086
0.415TrpTyr: 0.415 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.159TyrAla: 2.159 ± 0.39
0.249TyrCys: 0.249 ± 0.142
2.657TyrAsp: 2.657 ± 0.357
2.74TyrGlu: 2.74 ± 0.466
2.076TyrPhe: 2.076 ± 0.498
2.408TyrGly: 2.408 ± 0.565
1.08TyrHis: 1.08 ± 0.342
2.574TyrIle: 2.574 ± 0.626
3.405TyrLys: 3.405 ± 0.56
3.488TyrLeu: 3.488 ± 0.612
0.997TyrMet: 0.997 ± 0.299
2.574TyrAsn: 2.574 ± 0.527
0.913TyrPro: 0.913 ± 0.269
1.744TyrGln: 1.744 ± 0.434
1.827TyrArg: 1.827 ± 0.419
1.827TyrSer: 1.827 ± 0.332
1.08TyrThr: 1.08 ± 0.321
2.823TyrVal: 2.823 ± 0.592
0.581TyrTrp: 0.581 ± 0.203
1.827TyrTyr: 1.827 ± 0.373
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12043 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski