Thalassotalea mangrovi 
Average proteome isoelectric point is 6.03 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A4U1B4Z6|A0A4U1B4Z6_9GAMM Uncharacterized protein OS=Thalassotalea mangrovi OX=2572245 GN=E8M12_09540 PE=4 SV=1MM1 pKa = 7.9  EE2 pKa = 5.15  YY3 pKa = 8.91  NTSEE7 pKa = 4.19  LCNLYY12 pKa = 9.93  TDD14 pKa = 4.41  LVDD17 pKa = 3.64  VLEE20 pKa = 4.91  PMLSNYY26 pKa = 9.79  GGRR29 pKa = 11.84  SSFGGQVVTIKK40 pKa = 10.68  CFEE43 pKa = 4.5  ANGLISQMVEE53 pKa = 3.22  NDD55 pKa = 3.28  GTGKK59 pKa = 10.37  VLVIDD64 pKa = 4.47  GGGSARR70 pKa = 11.84  KK71 pKa = 9.52  ALVDD75 pKa = 3.61  SYY77 pKa = 11.0  IAEE80 pKa = 4.18  AAAKK84 pKa = 9.8  NGWEE88 pKa = 4.48  GIICYY93 pKa = 10.4  GSVRR97 pKa = 11.84  DD98 pKa = 3.69  VDD100 pKa = 3.85  ALEE103 pKa = 5.06  EE104 pKa = 4.08  IDD106 pKa = 4.71  IGIQAMVSIPVGAVDD121 pKa = 4.76  NDD123 pKa = 3.76  DD124 pKa = 4.53  GEE126 pKa = 4.96  IDD128 pKa = 3.77  VAVNFAGVTILPEE141 pKa = 3.69  DD142 pKa = 4.7  HH143 pKa = 7.24  IYY145 pKa = 11.29  ADD147 pKa = 3.46  NTGVVLSPDD156 pKa = 3.74  PLDD159 pKa = 4.11  IEE161 pKa = 4.46  
 17.02 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.715 
IPC2_protein 3.694 
IPC_protein 3.681 
Toseland    3.465 
ProMoST     3.821 
Dawson      3.668 
Bjellqvist  3.872 
Wikipedia   3.605 
Rodwell     3.503 
Grimsley    3.376 
Solomon     3.656 
Lehninger   3.617 
Nozaki      3.795 
DTASelect   4.012 
Thurlkill   3.528 
EMBOSS      3.617 
Sillero     3.795 
Patrickios  0.947 
IPC_peptide 3.656 
IPC2_peptide  3.77 
IPC2.peptide.svr19  3.752 
 Protein with the highest isoelectric point: 
>tr|A0A4U1B7S3|A0A4U1B7S3_9GAMM Hsp20 family protein OS=Thalassotalea mangrovi OX=2572245 GN=E8M12_05225 PE=3 SV=1MM1 pKa = 7.45  KK2 pKa = 9.51  RR3 pKa = 11.84  TFQPSVLKK11 pKa = 10.46  RR12 pKa = 11.84  KK13 pKa = 9.37  RR14 pKa = 11.84  NHH16 pKa = 5.37  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATKK25 pKa = 10.33  NGRR28 pKa = 11.84  AVIARR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 10.46  GRR39 pKa = 11.84  AKK41 pKa = 10.69  LSAA44 pKa = 3.92  
 5.08 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.517 
IPC2_protein 11.213 
IPC_protein 12.793 
Toseland    12.969 
ProMoST     13.451 
Dawson      12.969 
Bjellqvist  12.954 
Wikipedia   13.437 
Rodwell     12.676 
Grimsley    12.998 
Solomon     13.451 
Lehninger   13.364 
Nozaki      12.969 
DTASelect   12.954 
Thurlkill   12.969 
EMBOSS      13.466 
Sillero     12.969 
Patrickios  12.398 
IPC_peptide 13.466 
IPC2_peptide  12.442 
IPC2.peptide.svr19  9.148 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        3157 
0
3157 
1056890
25
1762
334.8
37.27
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.657 ± 0.046
1.011 ± 0.015
5.873 ± 0.036
6.013 ± 0.035
4.298 ± 0.03
6.852 ± 0.038
2.224 ± 0.023
6.445 ± 0.033
5.079 ± 0.037
10.212 ± 0.053
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.535 ± 0.018
4.446 ± 0.026
3.931 ± 0.027
5.135 ± 0.046
4.536 ± 0.031
6.379 ± 0.029
5.141 ± 0.028
6.772 ± 0.038
1.252 ± 0.015
3.208 ± 0.027
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here