Anabaena phage A-4L

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kozyakovvirus; Anabaena virus A4L

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059PYA9|A0A059PYA9_9CAUD Portal protein OS=Anabaena phage A-4L OX=1357732 GN=A4L_35 PE=4 SV=1
MM1 pKa = 7.5SFLPYY6 pKa = 10.33TLTKK10 pKa = 9.57GVNYY14 pKa = 10.45NVLYY18 pKa = 10.64DD19 pKa = 3.75NQFMFTGVFRR29 pKa = 11.84GYY31 pKa = 10.7DD32 pKa = 3.47YY33 pKa = 11.64SGDD36 pKa = 3.72RR37 pKa = 11.84ACLLRR42 pKa = 11.84EE43 pKa = 4.24GWSSLTYY50 pKa = 10.14IPNIPEE56 pKa = 3.84WTLVDD61 pKa = 3.46ATEE64 pKa = 4.24TPIEE68 pKa = 4.16EE69 pKa = 4.62EE70 pKa = 4.09EE71 pKa = 4.27VQPFF75 pKa = 3.26

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059PY20|A0A059PY20_9CAUD Uncharacterized protein OS=Anabaena phage A-4L OX=1357732 GN=A4L_07 PE=4 SV=1
MM1 pKa = 6.82VQRR4 pKa = 11.84TTPTNDD10 pKa = 2.53KK11 pKa = 11.12RR12 pKa = 11.84MTQLIASLITDD23 pKa = 3.75YY24 pKa = 11.67NDD26 pKa = 3.74GVITASEE33 pKa = 4.54LLRR36 pKa = 11.84LTQPYY41 pKa = 8.7IQARR45 pKa = 11.84SRR47 pKa = 11.84RR48 pKa = 11.84NLALPDD54 pKa = 3.63QLTEE58 pKa = 4.12ALRR61 pKa = 11.84HH62 pKa = 5.42AFNSIHH68 pKa = 6.48

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

12639

46

1717

332.6

37.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.58 ± 0.493

0.791 ± 0.166

5.705 ± 0.231

6.037 ± 0.404

3.212 ± 0.251

6.124 ± 0.347

1.582 ± 0.194

5.554 ± 0.235

4.89 ± 0.352

8.094 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.218

6.187 ± 0.24

4.716 ± 0.489

4.7 ± 0.456

5.902 ± 0.249

6.599 ± 0.32

7.69 ± 0.389

7.058 ± 0.272

1.361 ± 0.149

4.114 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski