Amino acid dipepetide frequency for Anabaena phage A-4L

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.042AlaAla: 7.042 ± 0.925
0.475AlaCys: 0.475 ± 0.216
3.956AlaAsp: 3.956 ± 0.37
4.51AlaGlu: 4.51 ± 0.679
2.532AlaPhe: 2.532 ± 0.48
3.561AlaGly: 3.561 ± 0.49
0.791AlaHis: 0.791 ± 0.236
5.381AlaIle: 5.381 ± 0.724
4.985AlaLys: 4.985 ± 0.692
6.726AlaLeu: 6.726 ± 0.848
1.503AlaMet: 1.503 ± 0.42
5.539AlaAsn: 5.539 ± 0.622
2.374AlaPro: 2.374 ± 0.708
3.323AlaGln: 3.323 ± 0.7
4.431AlaArg: 4.431 ± 0.596
6.014AlaSer: 6.014 ± 0.673
4.589AlaThr: 4.589 ± 1.055
5.301AlaVal: 5.301 ± 0.74
0.791AlaTrp: 0.791 ± 0.201
1.978AlaTyr: 1.978 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
0.475CysAla: 0.475 ± 0.179
0.158CysCys: 0.158 ± 0.104
0.317CysAsp: 0.317 ± 0.153
0.633CysGlu: 0.633 ± 0.32
0.079CysPhe: 0.079 ± 0.076
0.633CysGly: 0.633 ± 0.211
0.158CysHis: 0.158 ± 0.108
0.396CysIle: 0.396 ± 0.161
0.237CysLys: 0.237 ± 0.115
0.87CysLeu: 0.87 ± 0.241
0.237CysMet: 0.237 ± 0.126
0.317CysAsn: 0.317 ± 0.156
0.237CysPro: 0.237 ± 0.132
0.396CysGln: 0.396 ± 0.155
0.475CysArg: 0.475 ± 0.29
0.317CysSer: 0.317 ± 0.138
0.87CysThr: 0.87 ± 0.284
0.396CysVal: 0.396 ± 0.195
0.0CysTrp: 0.0 ± 0.0
0.712CysTyr: 0.712 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
5.539AspAla: 5.539 ± 0.866
0.317AspCys: 0.317 ± 0.169
4.352AspAsp: 4.352 ± 0.577
5.143AspGlu: 5.143 ± 0.79
1.899AspPhe: 1.899 ± 0.321
4.115AspGly: 4.115 ± 0.625
0.554AspHis: 0.554 ± 0.231
4.51AspIle: 4.51 ± 0.629
2.769AspLys: 2.769 ± 0.428
3.402AspLeu: 3.402 ± 0.338
0.791AspMet: 0.791 ± 0.233
3.323AspAsn: 3.323 ± 0.532
2.374AspPro: 2.374 ± 0.401
1.583AspGln: 1.583 ± 0.256
2.611AspArg: 2.611 ± 0.434
2.849AspSer: 2.849 ± 0.412
3.482AspThr: 3.482 ± 0.593
4.431AspVal: 4.431 ± 0.436
0.712AspTrp: 0.712 ± 0.144
2.295AspTyr: 2.295 ± 0.428
0.0AspXaa: 0.0 ± 0.0
Glu
5.776GluAla: 5.776 ± 0.851
0.396GluCys: 0.396 ± 0.228
2.136GluAsp: 2.136 ± 0.362
2.611GluGlu: 2.611 ± 0.489
2.453GluPhe: 2.453 ± 0.427
2.216GluGly: 2.216 ± 0.4
0.95GluHis: 0.95 ± 0.304
3.323GluIle: 3.323 ± 0.598
2.374GluLys: 2.374 ± 0.457
6.172GluLeu: 6.172 ± 0.857
1.424GluMet: 1.424 ± 0.395
2.057GluAsn: 2.057 ± 0.384
3.086GluPro: 3.086 ± 0.691
3.165GluGln: 3.165 ± 0.536
4.352GluArg: 4.352 ± 0.769
3.007GluSer: 3.007 ± 0.423
4.51GluThr: 4.51 ± 0.688
5.222GluVal: 5.222 ± 0.642
1.345GluTrp: 1.345 ± 0.218
3.798GluTyr: 3.798 ± 0.644
0.0GluXaa: 0.0 ± 0.0
Phe
2.611PheAla: 2.611 ± 0.492
0.317PheCys: 0.317 ± 0.158
2.928PheAsp: 2.928 ± 0.453
1.583PheGlu: 1.583 ± 0.323
0.633PhePhe: 0.633 ± 0.283
1.978PheGly: 1.978 ± 0.438
0.237PheHis: 0.237 ± 0.138
1.503PheIle: 1.503 ± 0.415
2.136PheLys: 2.136 ± 0.353
2.136PheLeu: 2.136 ± 0.425
1.266PheMet: 1.266 ± 0.355
3.165PheAsn: 3.165 ± 0.38
0.633PhePro: 0.633 ± 0.188
1.029PheGln: 1.029 ± 0.317
1.503PheArg: 1.503 ± 0.249
1.899PheSer: 1.899 ± 0.46
3.165PheThr: 3.165 ± 0.356
2.057PheVal: 2.057 ± 0.489
0.554PheTrp: 0.554 ± 0.19
0.791PheTyr: 0.791 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
4.589GlyAla: 4.589 ± 0.679
0.791GlyCys: 0.791 ± 0.248
3.798GlyAsp: 3.798 ± 0.415
3.007GlyGlu: 3.007 ± 0.529
2.057GlyPhe: 2.057 ± 0.331
4.906GlyGly: 4.906 ± 0.964
0.712GlyHis: 0.712 ± 0.286
3.165GlyIle: 3.165 ± 0.449
2.216GlyLys: 2.216 ± 0.425
6.172GlyLeu: 6.172 ± 0.712
1.108GlyMet: 1.108 ± 0.298
3.007GlyAsn: 3.007 ± 0.532
0.0GlyPro: 0.0 ± 0.0
2.69GlyGln: 2.69 ± 0.435
3.561GlyArg: 3.561 ± 0.429
4.985GlySer: 4.985 ± 0.838
4.827GlyThr: 4.827 ± 0.574
5.301GlyVal: 5.301 ± 0.663
0.87GlyTrp: 0.87 ± 0.236
3.482GlyTyr: 3.482 ± 0.528
0.0GlyXaa: 0.0 ± 0.0
His
1.266HisAla: 1.266 ± 0.285
0.396HisCys: 0.396 ± 0.189
0.87HisAsp: 0.87 ± 0.334
0.554HisGlu: 0.554 ± 0.181
0.554HisPhe: 0.554 ± 0.198
0.475HisGly: 0.475 ± 0.189
0.158HisHis: 0.158 ± 0.106
1.424HisIle: 1.424 ± 0.394
0.237HisLys: 0.237 ± 0.126
1.345HisLeu: 1.345 ± 0.265
0.158HisMet: 0.158 ± 0.106
0.87HisAsn: 0.87 ± 0.261
1.108HisPro: 1.108 ± 0.29
0.396HisGln: 0.396 ± 0.208
1.503HisArg: 1.503 ± 0.336
1.108HisSer: 1.108 ± 0.313
1.187HisThr: 1.187 ± 0.236
1.345HisVal: 1.345 ± 0.351
0.158HisTrp: 0.158 ± 0.1
0.712HisTyr: 0.712 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
3.64IleAla: 3.64 ± 0.476
0.317IleCys: 0.317 ± 0.141
4.827IleAsp: 4.827 ± 0.647
4.985IleGlu: 4.985 ± 0.578
1.108IlePhe: 1.108 ± 0.331
3.244IleGly: 3.244 ± 0.391
0.95IleHis: 0.95 ± 0.237
2.532IleIle: 2.532 ± 0.441
3.165IleLys: 3.165 ± 0.577
4.115IleLeu: 4.115 ± 0.622
0.95IleMet: 0.95 ± 0.187
4.51IleAsn: 4.51 ± 0.844
3.402IlePro: 3.402 ± 0.776
2.374IleGln: 2.374 ± 0.467
3.165IleArg: 3.165 ± 0.419
2.374IleSer: 2.374 ± 0.461
4.985IleThr: 4.985 ± 0.739
2.295IleVal: 2.295 ± 0.451
0.475IleTrp: 0.475 ± 0.201
2.136IleTyr: 2.136 ± 0.396
0.0IleXaa: 0.0 ± 0.0
Lys
3.877LysAla: 3.877 ± 0.467
0.317LysCys: 0.317 ± 0.156
1.899LysAsp: 1.899 ± 0.429
2.532LysGlu: 2.532 ± 0.426
2.136LysPhe: 2.136 ± 0.327
2.611LysGly: 2.611 ± 0.515
1.503LysHis: 1.503 ± 0.377
1.82LysIle: 1.82 ± 0.4
2.057LysLys: 2.057 ± 0.443
5.143LysLeu: 5.143 ± 0.883
0.95LysMet: 0.95 ± 0.358
1.583LysAsn: 1.583 ± 0.322
3.956LysPro: 3.956 ± 1.003
2.136LysGln: 2.136 ± 0.401
4.51LysArg: 4.51 ± 0.756
2.453LysSer: 2.453 ± 0.429
2.849LysThr: 2.849 ± 0.407
3.007LysVal: 3.007 ± 0.575
0.633LysTrp: 0.633 ± 0.252
2.928LysTyr: 2.928 ± 0.541
0.0LysXaa: 0.0 ± 0.0
Leu
6.172LeuAla: 6.172 ± 0.649
0.554LeuCys: 0.554 ± 0.227
5.222LeuAsp: 5.222 ± 0.749
4.748LeuGlu: 4.748 ± 0.758
3.086LeuPhe: 3.086 ± 0.751
5.46LeuGly: 5.46 ± 0.759
0.791LeuHis: 0.791 ± 0.286
4.194LeuIle: 4.194 ± 0.55
3.244LeuLys: 3.244 ± 0.457
7.438LeuLeu: 7.438 ± 0.557
2.216LeuMet: 2.216 ± 0.498
4.115LeuAsn: 4.115 ± 0.888
4.827LeuPro: 4.827 ± 0.715
3.086LeuGln: 3.086 ± 0.59
4.352LeuArg: 4.352 ± 0.592
5.143LeuSer: 5.143 ± 0.593
7.359LeuThr: 7.359 ± 0.768
5.222LeuVal: 5.222 ± 0.635
1.108LeuTrp: 1.108 ± 0.412
2.611LeuTyr: 2.611 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
1.503MetAla: 1.503 ± 0.399
0.158MetCys: 0.158 ± 0.139
1.108MetAsp: 1.108 ± 0.317
1.345MetGlu: 1.345 ± 0.351
0.554MetPhe: 0.554 ± 0.239
1.266MetGly: 1.266 ± 0.405
0.079MetHis: 0.079 ± 0.075
0.554MetIle: 0.554 ± 0.252
1.108MetLys: 1.108 ± 0.298
1.662MetLeu: 1.662 ± 0.537
0.396MetMet: 0.396 ± 0.147
0.712MetAsn: 0.712 ± 0.25
0.87MetPro: 0.87 ± 0.301
0.712MetGln: 0.712 ± 0.221
1.029MetArg: 1.029 ± 0.194
2.849MetSer: 2.849 ± 0.493
2.057MetThr: 2.057 ± 0.42
1.345MetVal: 1.345 ± 0.304
0.317MetTrp: 0.317 ± 0.14
1.424MetTyr: 1.424 ± 0.305
0.0MetXaa: 0.0 ± 0.0
Asn
4.985AsnAla: 4.985 ± 0.908
0.317AsnCys: 0.317 ± 0.131
4.51AsnAsp: 4.51 ± 0.573
3.719AsnGlu: 3.719 ± 0.585
1.82AsnPhe: 1.82 ± 0.347
4.352AsnGly: 4.352 ± 0.759
1.583AsnHis: 1.583 ± 0.371
3.561AsnIle: 3.561 ± 0.458
2.928AsnLys: 2.928 ± 0.649
3.323AsnLeu: 3.323 ± 0.375
0.712AsnMet: 0.712 ± 0.273
4.035AsnAsn: 4.035 ± 0.48
3.086AsnPro: 3.086 ± 0.623
2.69AsnGln: 2.69 ± 0.386
3.086AsnArg: 3.086 ± 0.417
4.352AsnSer: 4.352 ± 0.523
5.222AsnThr: 5.222 ± 0.745
4.035AsnVal: 4.035 ± 0.488
1.187AsnTrp: 1.187 ± 0.309
2.374AsnTyr: 2.374 ± 0.412
0.0AsnXaa: 0.0 ± 0.0
Pro
2.295ProAla: 2.295 ± 0.428
0.475ProCys: 0.475 ± 0.152
3.165ProAsp: 3.165 ± 0.626
4.194ProGlu: 4.194 ± 0.763
1.187ProPhe: 1.187 ± 0.4
2.611ProGly: 2.611 ± 0.48
1.345ProHis: 1.345 ± 0.277
2.136ProIle: 2.136 ± 0.513
2.453ProLys: 2.453 ± 0.57
3.165ProLeu: 3.165 ± 0.762
0.633ProMet: 0.633 ± 0.188
3.798ProAsn: 3.798 ± 0.671
1.82ProPro: 1.82 ± 0.41
2.136ProGln: 2.136 ± 0.467
1.662ProArg: 1.662 ± 0.401
3.561ProSer: 3.561 ± 0.838
4.985ProThr: 4.985 ± 0.972
3.165ProVal: 3.165 ± 0.611
0.317ProTrp: 0.317 ± 0.131
2.057ProTyr: 2.057 ± 0.475
0.0ProXaa: 0.0 ± 0.0
Gln
4.035GlnAla: 4.035 ± 0.874
0.237GlnCys: 0.237 ± 0.123
1.187GlnAsp: 1.187 ± 0.266
1.503GlnGlu: 1.503 ± 0.343
1.741GlnPhe: 1.741 ± 0.319
2.057GlnGly: 2.057 ± 0.289
0.317GlnHis: 0.317 ± 0.125
1.583GlnIle: 1.583 ± 0.33
2.374GlnLys: 2.374 ± 0.489
5.618GlnLeu: 5.618 ± 0.725
1.266GlnMet: 1.266 ± 0.409
2.136GlnAsn: 2.136 ± 0.577
3.165GlnPro: 3.165 ± 0.908
5.064GlnGln: 5.064 ± 1.563
3.482GlnArg: 3.482 ± 0.471
2.374GlnSer: 2.374 ± 0.426
3.482GlnThr: 3.482 ± 0.597
2.769GlnVal: 2.769 ± 0.484
0.633GlnTrp: 0.633 ± 0.199
1.978GlnTyr: 1.978 ± 0.427
0.0GlnXaa: 0.0 ± 0.0
Arg
4.431ArgAla: 4.431 ± 0.63
0.475ArgCys: 0.475 ± 0.171
2.769ArgAsp: 2.769 ± 0.324
4.748ArgGlu: 4.748 ± 0.619
1.583ArgPhe: 1.583 ± 0.289
2.928ArgGly: 2.928 ± 0.412
1.108ArgHis: 1.108 ± 0.321
2.769ArgIle: 2.769 ± 0.432
3.244ArgLys: 3.244 ± 0.496
5.143ArgLeu: 5.143 ± 0.473
1.662ArgMet: 1.662 ± 0.281
3.086ArgAsn: 3.086 ± 0.37
2.136ArgPro: 2.136 ± 0.37
3.561ArgGln: 3.561 ± 0.541
4.589ArgArg: 4.589 ± 0.57
2.769ArgSer: 2.769 ± 0.433
3.877ArgThr: 3.877 ± 0.512
5.301ArgVal: 5.301 ± 0.847
0.633ArgTrp: 0.633 ± 0.21
2.136ArgTyr: 2.136 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
4.115SerAla: 4.115 ± 0.573
0.158SerCys: 0.158 ± 0.121
4.035SerAsp: 4.035 ± 0.527
4.273SerGlu: 4.273 ± 0.684
2.295SerPhe: 2.295 ± 0.37
4.51SerGly: 4.51 ± 0.697
1.583SerHis: 1.583 ± 0.329
3.877SerIle: 3.877 ± 0.671
3.482SerLys: 3.482 ± 0.614
4.827SerLeu: 4.827 ± 0.636
1.662SerMet: 1.662 ± 0.434
3.086SerAsn: 3.086 ± 0.707
3.086SerPro: 3.086 ± 0.535
3.64SerGln: 3.64 ± 0.737
4.273SerArg: 4.273 ± 0.375
5.539SerSer: 5.539 ± 0.838
4.273SerThr: 4.273 ± 0.696
4.352SerVal: 4.352 ± 0.693
0.633SerTrp: 0.633 ± 0.266
2.295SerTyr: 2.295 ± 0.414
0.0SerXaa: 0.0 ± 0.0
Thr
5.46ThrAla: 5.46 ± 0.963
0.396ThrCys: 0.396 ± 0.2
3.244ThrAsp: 3.244 ± 0.436
4.035ThrGlu: 4.035 ± 0.638
2.216ThrPhe: 2.216 ± 0.488
6.093ThrGly: 6.093 ± 0.828
1.266ThrHis: 1.266 ± 0.252
3.798ThrIle: 3.798 ± 0.456
3.086ThrLys: 3.086 ± 0.338
4.985ThrLeu: 4.985 ± 0.806
1.187ThrMet: 1.187 ± 0.295
5.776ThrAsn: 5.776 ± 0.665
6.251ThrPro: 6.251 ± 1.069
3.956ThrGln: 3.956 ± 0.834
3.719ThrArg: 3.719 ± 0.569
5.222ThrSer: 5.222 ± 0.796
6.963ThrThr: 6.963 ± 1.468
5.222ThrVal: 5.222 ± 0.53
1.503ThrTrp: 1.503 ± 0.327
2.532ThrTyr: 2.532 ± 0.655
0.0ThrXaa: 0.0 ± 0.0
Val
4.115ValAla: 4.115 ± 0.429
0.712ValCys: 0.712 ± 0.266
3.561ValAsp: 3.561 ± 0.556
3.323ValGlu: 3.323 ± 0.4
2.453ValPhe: 2.453 ± 0.427
4.194ValGly: 4.194 ± 0.457
0.791ValHis: 0.791 ± 0.187
5.934ValIle: 5.934 ± 1.061
3.719ValLys: 3.719 ± 0.546
4.589ValLeu: 4.589 ± 0.802
1.503ValMet: 1.503 ± 0.362
6.33ValAsn: 6.33 ± 1.055
3.086ValPro: 3.086 ± 0.39
2.69ValGln: 2.69 ± 0.75
3.719ValArg: 3.719 ± 0.566
5.618ValSer: 5.618 ± 0.689
4.589ValThr: 4.589 ± 0.837
5.934ValVal: 5.934 ± 0.771
0.87ValTrp: 0.87 ± 0.272
2.849ValTyr: 2.849 ± 0.624
0.0ValXaa: 0.0 ± 0.0
Trp
0.95TrpAla: 0.95 ± 0.225
0.554TrpCys: 0.554 ± 0.22
0.633TrpAsp: 0.633 ± 0.203
0.791TrpGlu: 0.791 ± 0.239
0.554TrpPhe: 0.554 ± 0.221
0.633TrpGly: 0.633 ± 0.165
0.079TrpHis: 0.079 ± 0.071
0.158TrpIle: 0.158 ± 0.107
0.712TrpLys: 0.712 ± 0.186
1.503TrpLeu: 1.503 ± 0.433
0.554TrpMet: 0.554 ± 0.222
1.266TrpAsn: 1.266 ± 0.379
0.0TrpPro: 0.0 ± 0.0
0.554TrpGln: 0.554 ± 0.212
0.87TrpArg: 0.87 ± 0.24
1.345TrpSer: 1.345 ± 0.282
0.87TrpThr: 0.87 ± 0.215
0.633TrpVal: 0.633 ± 0.191
0.237TrpTrp: 0.237 ± 0.139
0.712TrpTyr: 0.712 ± 0.288
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.928TyrAla: 2.928 ± 0.648
0.396TyrCys: 0.396 ± 0.18
2.532TyrAsp: 2.532 ± 0.36
2.136TyrGlu: 2.136 ± 0.386
1.187TyrPhe: 1.187 ± 0.261
3.402TyrGly: 3.402 ± 0.567
0.87TyrHis: 0.87 ± 0.29
2.928TyrIle: 2.928 ± 0.462
2.374TyrLys: 2.374 ± 0.512
2.611TyrLeu: 2.611 ± 0.472
0.791TyrMet: 0.791 ± 0.251
3.165TyrAsn: 3.165 ± 0.624
1.662TyrPro: 1.662 ± 0.268
1.82TyrGln: 1.82 ± 0.488
2.136TyrArg: 2.136 ± 0.452
2.216TyrSer: 2.216 ± 0.471
2.769TyrThr: 2.769 ± 0.552
3.244TyrVal: 3.244 ± 0.462
0.633TyrTrp: 0.633 ± 0.202
1.345TyrTyr: 1.345 ± 0.384
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (12639 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski