Choanephora cucurbitarum
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11924 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C7LQE7|A0A1C7LQE7_9FUNG Uncharacterized protein (Fragment) OS=Choanephora cucurbitarum OX=101091 GN=A0J61_11950 PE=4 SV=1
MM1 pKa = 8.25 DD2 pKa = 4.39 SLFYY6 pKa = 11.24 LLFIDD11 pKa = 4.31 EE12 pKa = 5.72 FIVSSGITLQSIASSLALQANIPGEE37 pKa = 4.85 DD38 pKa = 3.57 IANMSDD44 pKa = 2.59 WSLPTTFEE52 pKa = 3.44 NCYY55 pKa = 10.38 QRR57 pKa = 11.84 DD58 pKa = 3.67 HH59 pKa = 6.77 LSCFKK64 pKa = 10.36 FANILITPSFKK75 pKa = 9.79 IDD77 pKa = 3.21 IDD79 pKa = 3.52 IHH81 pKa = 7.93 LDD83 pKa = 3.67 DD84 pKa = 4.15 YY85 pKa = 11.18 TDD87 pKa = 3.71 VYY89 pKa = 10.89 FKK91 pKa = 11.21 SLDD94 pKa = 3.35
Molecular weight: 10.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.63
ProMoST 3.973
Dawson 3.872
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.279
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 0.846
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|A0A1C7NMY3|A0A1C7NMY3_9FUNG Papain inhibitor OS=Choanephora cucurbitarum OX=101091 GN=pi_0 PE=4 SV=1
MM1 pKa = 7.61 GNNLSSTNRR10 pKa = 11.84 SSKK13 pKa = 9.77 QRR15 pKa = 11.84 NTRR18 pKa = 11.84 KK19 pKa = 10.03 VKK21 pKa = 10.25 ASNRR25 pKa = 11.84 AKK27 pKa = 9.65 TPKK30 pKa = 9.83 KK31 pKa = 10.14 RR32 pKa = 11.84 FSLTPQKK39 pKa = 10.65 QVGTAPSTSNGLRR52 pKa = 11.84 RR53 pKa = 11.84 LSLGSSKK60 pKa = 10.49 KK61 pKa = 10.27 GKK63 pKa = 9.95 QPAKK67 pKa = 10.04 SNKK70 pKa = 9.52 FGLSMNNKK78 pKa = 7.81 RR79 pKa = 11.84 DD80 pKa = 3.46 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 SSVASNGSFGKK94 pKa = 9.87 LKK96 pKa = 11.31 AMMTPSGTATKK107 pKa = 9.86 KK108 pKa = 9.35 QKK110 pKa = 9.62 KK111 pKa = 7.92 SKK113 pKa = 9.86 RR114 pKa = 11.84 RR115 pKa = 11.84 SSTADD120 pKa = 2.81 RR121 pKa = 11.84 VMDD124 pKa = 3.96 KK125 pKa = 11.08 LNPNVNTAQTSKK137 pKa = 11.05 AKK139 pKa = 10.22 RR140 pKa = 11.84 NSVSNPFKK148 pKa = 10.68 KK149 pKa = 10.32 QPTKK153 pKa = 10.77 QRR155 pKa = 11.84 GFMSKK160 pKa = 9.34 VAAA163 pKa = 4.33
Molecular weight: 17.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.818
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.486
Grimsley 12.559
Solomon 12.983
Lehninger 12.896
Nozaki 12.515
DTASelect 12.486
Thurlkill 12.515
EMBOSS 12.998
Sillero 12.515
Patrickios 12.193
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.97
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11924
0
11924
4748132
9
5983
398.2
45.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.565 ± 0.021
1.402 ± 0.009
5.686 ± 0.015
6.557 ± 0.025
4.026 ± 0.015
4.774 ± 0.023
2.719 ± 0.011
5.909 ± 0.016
6.336 ± 0.024
9.364 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.01
4.681 ± 0.015
4.835 ± 0.02
4.948 ± 0.018
5.036 ± 0.017
8.295 ± 0.029
6.026 ± 0.016
5.862 ± 0.019
1.138 ± 0.007
3.359 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here