Capra hircus (Goat)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32615 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A452EBZ2|A0A452EBZ2_CAPHI Olfactory receptor OS=Capra hircus OX=9925 GN=LOC102188399 PE=3 SV=1
MM1 pKa = 8.24 DD2 pKa = 4.75 FQQLADD8 pKa = 4.22 VAEE11 pKa = 4.71 KK12 pKa = 9.75 WCSNTPFEE20 pKa = 5.73 LIATEE25 pKa = 3.96 EE26 pKa = 4.32 TEE28 pKa = 4.33 RR29 pKa = 11.84 RR30 pKa = 11.84 MDD32 pKa = 4.29 FYY34 pKa = 11.47 ADD36 pKa = 3.74 PGVSFYY42 pKa = 10.98 VLCPDD47 pKa = 4.64 NGCGDD52 pKa = 3.96 NFHH55 pKa = 6.3 VWSEE59 pKa = 4.48 SEE61 pKa = 3.97 DD62 pKa = 3.63 CLPFLQLAQDD72 pKa = 4.45 YY73 pKa = 10.71 ISSCGKK79 pKa = 8.56 KK80 pKa = 7.94 TLHH83 pKa = 6.31 EE84 pKa = 4.11 VLEE87 pKa = 4.47 KK88 pKa = 10.61 VFKK91 pKa = 10.63 SFRR94 pKa = 11.84 PLLGLPDD101 pKa = 4.83 ADD103 pKa = 3.93 DD104 pKa = 4.59 DD105 pKa = 4.62 AFEE108 pKa = 4.77 EE109 pKa = 4.52 YY110 pKa = 10.51 SADD113 pKa = 3.68 VEE115 pKa = 4.38 EE116 pKa = 5.09 EE117 pKa = 4.2 EE118 pKa = 5.29 PEE120 pKa = 4.13 ADD122 pKa = 3.66 HH123 pKa = 6.65 PQMGVSQQQ131 pKa = 3.0
Molecular weight: 14.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.706
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.617
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.164
Thurlkill 3.745
EMBOSS 3.795
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A452G0F3|A0A452G0F3_CAPHI HEAT repeat containing 5B OS=Capra hircus OX=9925 GN=HEATR5B PE=3 SV=1
MM1 pKa = 7.67 RR2 pKa = 11.84 FHH4 pKa = 7.61 RR5 pKa = 11.84 FFLLFVMFMMSLLLIHH21 pKa = 6.58 GQRR24 pKa = 11.84 KK25 pKa = 9.45 ANLAMRR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 9.24 LHH35 pKa = 5.8 RR36 pKa = 11.84 HH37 pKa = 5.41 NGLKK41 pKa = 9.94 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 IPLHH48 pKa = 5.91 SRR50 pKa = 11.84 VPFPP54 pKa = 4.82
Molecular weight: 6.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.574
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21154
11461
32615
19787655
12
8801
606.7
67.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.074 ± 0.015
2.219 ± 0.012
4.861 ± 0.01
7.106 ± 0.018
3.662 ± 0.01
6.538 ± 0.018
2.556 ± 0.007
4.327 ± 0.011
5.699 ± 0.016
9.987 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.101 ± 0.006
3.561 ± 0.009
6.294 ± 0.02
4.738 ± 0.014
5.723 ± 0.012
8.372 ± 0.017
5.245 ± 0.009
6.082 ± 0.011
1.199 ± 0.005
2.654 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here